• 제목/요약/키워드: Medium test

검색결과 2,375건 처리시간 0.034초

폐 영구자석으로부터 회수한 염화희토류용액에서 PC88A를 이용한 경희토류(Pr, Nd)/중희토류(Tb, Dy) 용매추출 (Solvent Extraction of Light (Pr, Nd) and Medium (Tb, Dy) Rare Earth Elements with PC88A of Rare Earth Chloride Solution from Waste Permanent Magnet)

  • 전수병;손인준;임병철;김정모;김연진;하태규;윤호성;김철주;정경우
    • 자원리싸이클링
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    • 제27권3호
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    • pp.8-15
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    • 2018
  • Nd-Fe-B 폐 영구자석으로부터 회수한 염화희토류용액을 PC88A에 의한 경희토류(Pr, Nd)/중희토류(Tb, Dy) 분리정제에 대한 용매추출 연구를 진행하였다. 회분식 실험 단계에서 추출제(PC88A) 농도 0.5 M, 추출 후 평형 pH는 0.8~1.0(feed의 초기 pH 1.3), 세정액(HCl)의 농도는 0.1 M, 탈거액(HCl)의 농도는 2 M 이상일 때 분리 효율이 좋은 것을 확인하였다. 이 결과값을 혼합침강조에 적용하여 초기 조건에서 운용 조건을 변화시키면서 180시간 운용하여 3N급의 경희토류(Pr, Nd)/중희토류(Tb, Dy)금속 이온으로 분리정제 후 회수하였다.

Bacillus amyloliquefaciens ISP-5 균주의 배지 최적화 및 상추를 이용한 식물 생장 촉진 평가 (Medium optimization for growth of Bacillus amyloliquefaciens ISP-5 strain and evaluation of plant growth promotion using lettuce)

  • 최강현;서선일;박해성;임지환;김평일
    • Journal of Plant Biotechnology
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    • 제49권4호
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    • pp.356-361
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    • 2022
  • 본 연구에서는 Bacillus amyloliquefaciens ISP-5 균주의 최적배지조성을 확인하기 위해 5종의 탄소원(glucose, fructose, sucrose, maltose, corn starch)과 5종의 질소원(yeast extract, peptone, tryptone, soytone, soy bean flour)을 이용하여 각각의 생육을 측정하였다. 그 결과 glucose와 soy bean flour를 각각 최적 탄소원과 질소원으로 확립하였으며 최적 배지를 이용하였을 때 상업용 배지(TSB)에 비해 약 2500배의 생육을 확인할 수 있었다. B. amyloliquefaciens ISP-5 배양액을 상추에 엽면 살포하였을 때 상추의 엽폭과 엽장은 대조군에 비해 8.6%, 12.9%의 증가하였으며, 생체중과 건물중은 대조군에 비해 각각 24.2%, 23.9% 증가하였다. 이러한 결과들을 종합해보았을 때 B. amyloloiquefaciens ISP-5 균주를 이용한 미생물 제제는 상추용 식물생장촉진제로써 활용할 수 있을 것이라 기대된다.

NONTHERMAL RADIATION FROM RELATIVISTIC ELECTRONS ACCELERATED AT SPHERICALLY EXPANDING SHOCKS

  • Kang, Hyesung
    • 천문학회지
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    • 제48권1호
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    • pp.9-20
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    • 2015
  • We study the evolution of the energy spectrum of cosmic-ray electrons accelerated at spherically expanding shocks with low Mach numbers and the ensuing spectral signatures imprinted in radio synchrotron emission. Time-dependent simulations of diffusive shock acceleration (DSA) of electrons in the test-particle limit have been performed for spherical shocks with parameters relevant for typical shocks in the intracluster medium. The electron and radiation spectra at the shock location can be described properly by the test-particle DSA predictions with instantaneous shock parameters. However, the volume integrated spectra of both electrons and radiation deviate significantly from the test-particle power-laws, because the shock compression ratio and the flux of injected electrons at the shock gradually decrease as the shock slows down in time. So one needs to be cautious about interpreting observed radio spectra of evolving shocks based on simple DSA models in the test-particle regime.

Effects of Defect Size on Crush Test Load of Butt Fusion Welded MDPE Pipes

  • Tun, Nwe Ni;Lai, Huan Sheng;Jeon, Gyu Min;Yoon, Kee Bong;Kil, Seong Hee
    • 에너지공학
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    • 제24권4호
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    • pp.55-62
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    • 2015
  • It is expected that the size of welding defect affects the mechanical performance of welded medium density polyethylene (MDPE) pipe joints. In this study, butt fusion welded MDPE pipe joints with a single spherical or planar defect of various sizes were studied using experimental crush testing and also by finite element method. The crush test showed that the mechanical performance of crush was not affected by the size and geometry of a single welding defect when the defect size was increased to 45% of the pipe's wall thickness. The simulation results indicated that the effect of the single welding defect on the Von Mises stress distribution near the defect explained the reason of the test results.

HMC05의 배양 Chinese Hamster Lung 세포를 이용한 염색체이상 시험 (A Chromosome Aberration Test of HMC05 on Cultured Chinese Hamster Lung Cells)

  • 신흥묵
    • 대한본초학회지
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    • 제25권1호
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    • pp.1-7
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    • 2010
  • Objectives : We investigated genetic toxicity of HMCO5 in relation to chromosome aberration test on Cultured Chinese Hamster Lung (CHL) in the presence and absence of S-9 mix. Methods : Experimental groups were divided into two groups: with S-9mix (+S) or without S-9 mix (-S). -S group was also divided 2 series by treatment hours (6 hr: 6-S; or 24 hr; 24-S). Each group treated with vehicle only (complete culture medium), HMCO5 (1,250, 2,500, $5,000\;{\mu}g/ml$), and cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS), respectively. Results : HMC05 did not show any aberrant metaphase. However, there were significant (p < 0.01) aberrant metaphases with CPA in S+ and with EMS in S-. Conclusions : These results indicate that HMC05 formula does not show any toxicity in chromosome aberration test.

반복삼축압축시험을 이용한 주문진 표준사의 동적변형특성 분석 (Dynamic Deformation Characteristics of Joomunjin Standard Sand Using Cyclic Triaxial Test)

  • 김유성;고형우;김재홍;이진광
    • 한국지반공학회논문집
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    • 제28권12호
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    • pp.53-64
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    • 2012
  • 본 논문에서는 주문진 표준사를 대상으로 개량된 반복삼축압축 시험장치를 이용하여 탄성계수와 감쇠비에 대한 변형특성을 분석하였다. 기존의 낮은 정밀도를 갖는 LVDT를 사용한 반복삼축시험기를 개량하여 정밀하고 넓은 영역의 동적변형특성을 얻기 위해서 전단변형률(${\gamma}$) $10^{-4}-10^{-1}%$ 범위의 측정을 제어하는 셀 외부 LDT와 내부의 시료에 LDT를 설치하여 사용하였다. 이러한 미소변형제어를 통하여 5가지의 재하속도 변화와 간극비를 구분하고 4가지의 구속응력 조건을 달리하여 반복삼축시험을 수행하였다. 시험결과를 바탕으로 전단탄성계수와 감쇠비를 여러 조건에 대하여 반복하중 및 변형률 크기의 영향을 비교하고 분석한 결과, 주문진 표준사의 기존(공진주 시험)의 동적변형특성 보다 넓은 구간에서 시험값들을 얻을 수 있었다. 또한, 공진주 시험에서 0.02% 이하의 미소변형률 구간에서 얻은 결과를 토대로 중간변형률 구간을 비선형 모델로 예측한 값들은 반복삼축시험으로 얻은 실험값들과 다른 변형특성을 나타내고 있음을 관찰할 수 있었다.

유한 요소 시뮬레이션을 이용한 이중 매질 심부 체온계의 정확도 평가 (Accuracy Evaluation of Bi-medium Deep Body Thermometer Based on Finite Element Simulation)

  • 심수영;유호석;김한별;정재훈;이상준;김성민;박광석
    • 대한의용생체공학회:의공학회지
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    • 제35권5호
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    • pp.160-168
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    • 2014
  • Continuous body temperature monitoring is useful and essential in diverse medical procedures such as infection onset detection, therapeutic hypothermia, circadian rhythm monitoring, sleep disorder assessment, and gynecological research. However, the existing thermometers are too invasive or intrusive to be applied to long-term body temperature monitoring. In our previous study, we invented the bi-medium deep body thermometer which can noninvasively and continuously monitor deep tissue temperature. And the ratio of thermal resistances expressed as K-value should be obtained to estimate body temperature with the thermometer and it can be different under various measurement environments. Although the device was proven to be useful through preliminary simulation test and small group of human study, the experimental environment was restrictive in our previous approach. In this study, a finite element simulation was executed to obtain the K-value and evaluate the accuracy of bi-medium thermometer under various measurement environments. In addition, K-value estimation equation was developed by analyzing the influence of 5 measurement environmental factors (medium length, medium height, tissue depth, blood perfusion rate, and ambient temperature) on K-value. The results revealed that the estimation accuracy of bi-medium deep body thermometer based on computer simulation was very high (RMSE < $0.003^{\circ}C$) in various measurement environments. Also, bi-medium deep body thermometer based on K-value estimation equation showed relatively accurate results (RMSE < $0.3^{\circ}C$) except for one case. Although the K-value estimation technology should be improved for more accurate body temperature estimation, the results of finite element simulation showed that bi-medium deep body thermometer could accurately measure various tissue temperatures under diverse environments.

D-Methionine and 2-hydroxy-4-methylthiobutanoic acid i alter beta-casein, proteins and metabolites linked in milk protein synthesis in bovine mammary epithelial cells

  • Seung-Woo, Jeon;Jay Ronel V., Conejos;Jae-Sung, Lee;Sang-Hoon, Keum;Hong-Gu, Lee
    • Journal of Animal Science and Technology
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    • 제64권3호
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    • pp.481-499
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    • 2022
  • This study aims to determine the effects of D-methionine (D-Met) isomer and the methionine precursor 2-hydroxy-4-methylthiobutanoic acid i (HMBi) supplementation on milk protein synthesis on immortalized bovine mammary epithelial cell (MAC-T). MAC-T cells were seeded using 10-cm dishes and cultured in Dulbecco's modified Eagle's medium/F12 (DMEM/F12) basic medium. The basic medium of DMEM/F12 was replaced with the lactogenic DMEM/ F12 differentiation medium when 90% of MAC-T cells reached confluency. The best dosage at 0.6 mM of D-Met and HMBi and incubation time at 72 h were used uniformly for all treatments. Each treatment was replicated six times wherein treatments were randomly assigned in a 6-well plate. Cell, medium, and total protein were determined using a bicinchoninic acid protein assay kit. Genes, proteomics and metabolomics analyses were also done to determine the mechanism of the milk protein synthesis pathway. Data were analyzed by two-way analysis of variance (ANOVA) with supplement type and plate as fixed effects. The least significant difference test was used to evaluate the differences among treatments. The HMBi treatment group had the highest beta-casein and S6 kinase beta-1 (S6K1) mRNA gene expression levels. HMBi and D-Met treatments have higher gene expressions compared to the control group. In terms of medium protein content, HMBi had a higher medium protein quantity than the control although not significantly different from the D-Met group. HMBi supplementation stimulated the production of eukaryotic translation initiation factor 3 subunit protein essential for protein translation initiation resulting in higher medium protein synthesis in the HMBi group than in the control group. The protein pathway analysis results showed that the D-Met group stimulated fructose-galactose metabolism, glycolysis pathway, phosphoinositide 3 kinase, and pyruvate metabolism. The HMBi group stimulated the pentose phosphate and glycolysis pathways. Metabolite analysis revealed that the D-Met treatment group increased seven metabolites and decreased uridine monophosphate (UMP) production. HMBi supplementation increased the production of three metabolites and decreased UMP and N-acetyl-L-glutamate production. Taken together, D-Met and HMBi supplementation are effective in stimulating milk protein synthesis in MAC-T cells by genes, proteins, and metabolites stimulation linked to milk protein synthesis.

RBS 철골모멘트접합부의 내진거동평가를 위한 반복재하 실물대 실험 (Cyclic Seismic Testing of Full-Scale RBS (Reduced Beam Section) Steel Moment Connections)

  • 이철호;전상우;김진호
    • 한국지진공학회:학술대회논문집
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    • 한국지진공학회 2002년도 춘계 학술발표회 논문집
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    • pp.306-316
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    • 2002
  • This paper summarizes the results of full-scale cyclic seismic testing on four RBS (reduced beam section) steel moment connections. Key test variables were web bolting vs. welding and strong vs. medium PZ (panel zone) strength. The specimen with medium PZ strength was specially designed to mobilize energy dissipation from both the PZ and RBS region in a balanced way; the aim was to reduce the requirement of expensive doubler plates. Both strong and medium PZ specimens with web-welding were able to provide sufficient connection rotation capacity required of special moment frames, whereas specimens with web-bolting showed inferior performance due to the premature brittle fracture of the beam flange across the weld access hole. In contrast to the case of web-welded specimens, the web-bolted specimens could not transfer the actual plastic moment of the original (or unreduced) beam section to the column. If a quality welding for the beam-to-column joint is made as in this study, the fracture-prone area tends to move into the beam flange base metal within the weld access hole. Analytical study was also conducted to understand the observed base metal fracture from the engineering mechanics point of view.

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중소병원 간호사의 감정노동과 간호전문직관이 직무만족에 미치는 영향 (Influence of Emotional Labor and Nursing Professional Values on Job Satisfaction in Small and Medium-sized Hospital Nurses)

  • 류영옥;고은
    • 기본간호학회지
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    • 제22권1호
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    • pp.7-15
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    • 2015
  • Purpose: This study was done to investigate the correlation among emotional labor, nursing professional values, and job satisfaction and identify factors that affect job satisfaction of nurses working in small and medium-sized hospitals. Methods: Data were collected from 214 nurses in S city. The following measurement tools were used: Emotional labor by Morris et al. (1996), Nursing Professional Values Scale by Yeun et al. (2005), and Index of Work Satisfaction by Stamps et al. (1978). Collected data were analyzed with SPSS/WIN 18.0 using descriptive statistics, t-test, ANOVA, Pearson correlation coefficient, and multiple regression. Results: Participants' scores for emotional labor, nursing professional values and job satisfaction were $3.11{\pm}0.48$, $3.46{\pm}0.39$, and $3.02{\pm}0.29$ respectively. Emotional labor showed a negative correlation with job satisfaction (r=-.33, p<.001), while nursing professional values showed a positive correlation with job satisfaction (r=.58, p<.001). Emotional labor and nursing professional values were identified as factors influencing job satisfaction. The model explained about 39% of the variance in job satisfaction (F=67.40, p<.001). Conclusion: The results of this study suggest that it is important to manage emotional labor as well as to improve professional values and job satisfaction of nurses working in small and medium-sized hospital.