• Title/Summary/Keyword: Medium reactor

Search Result 208, Processing Time 0.027 seconds

Performance of a Novel Bioreactor Equipped with Moving Membrane Tube-Aeration System (회전하는 산소전달장치가 부착된 동물세포 배양기의 조업 성능에 관한 고찰)

  • Kim, Young-Nam;Jeon, Byung-Cheol;Lee, Jin-Ha;Lee, Hyeon-Yong
    • Microbiology and Biotechnology Letters
    • /
    • v.21 no.4
    • /
    • pp.348-353
    • /
    • 1993
  • The optimal conditions for operating a moving-aeration bioreactor were determined as 30rpm and 150 (ml/min) of air flow rate, which can yield ca. 7.3 (l/h)of maximum mass transfer coefficient. It was also found that the agitation speed played much much important role than air input rate in oxgen transfer into the medium. $2.6{\times}10^6$ (cells/ml) and 0.6 (ml/l) of maximum cell denisty and IL-2 production were observed in batch cultivation of IL-2 producing BHK cell line. 0.53 (mM/l/h) of oxygen uptake rate was also estimated. The performance of a moving-aeration bioreactor (specific growth rate and oxygen uptake rate, etc.) was superior to other culture systems, such as cell-life and static membrane aeration bioreactors. Ii must be useful to apply this reactor to many culture processes by improving structural limitations in scaling-up the system.

  • PDF

Cell-Recycled Continuous Ethanol Fermentation of Molasses (당밀의 균체순환식 Ethanol 연속발효)

  • 김익환;김병홍;민태익
    • Microbiology and Biotechnology Letters
    • /
    • v.10 no.2
    • /
    • pp.155-162
    • /
    • 1982
  • A cell-recycled continuous fermentation process was studied to produce ethanol from molasses using Sacchoromyces uvarum ATCC26602 at 35 $^{\circ}C$. The fermentation system was divided into two stages in order to reduce the inhibitions of ethanol and substrate for cell growth and fermentation rate. The first reactor was aerated at the rate of 0.12 vvm whilst the second was kept anaerobic. In medium composition studies, it was revealed that inorganic nutrient supplement to the diluted molasses with 14% fermentable sugar was not needed for the fermentation, however, phosphate limitation was observed when cell propagation was contemplated. By using the cell-recycled continuous fermentation system, 14.5 hour was required to produce 8.4-9.0% (v/v) of ethanol from diluted molasses containing 14% of fermentable sugar. The ethanol productivity was 6.2g/$\ell$hr with the yield of 88.1-94.4% to the theoretical value.

  • PDF

Removal of Toluene Vapor by Porous Ceramic Biofilter (다공성 세라믹 Biofilter를 이용한 Toluene 가스 제거)

  • Im, Jae-Sin;Gu, Ja-Gong;Park, Sang-Jin
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.14 no.6
    • /
    • pp.599-606
    • /
    • 1998
  • Removal of toluene vapor from airstreams was studied in a biological reactor known as a biofilter. The biofilter was packed porous ceramic inoculated with thickened activated sludge (MLVSS 17,683 mg/L). The lab-scale biofilter was operated for 42 days under various experimental conditions including inlet toluene concentrations and flow rates of the contaminated air streams. Removal efficiency reached up 96.6% after 4 days from start up. Nutrient limitation was proposed as a reason for the decrease in biofilter performence. Biofilter performance decreased substantially, coincident with the buildup of back pressure due to accumulation of excess VSS within the medium bed. Practically, the bed needs to be backwashed when the overall pressure drop is greater than 460.6 Pa at SV (Space Velocity) 100 h-1. Periodic backwashing of the biofilter was necessary for removing excess biomass and attaining stable long -term high removal efficiency The removal efficiency of toluene in the biofilter decreased as the gas velocity and toluene concentration in the inlet gas increased. The maximum elimination capacity of ceramic biofilter could reach up to 444.85 g/m3. hr. When the loading of toluene exceed this critical value, substrate inhibition occurred.

  • PDF

Continuous Ethanol Production Using immobilized Baker's Yeast (고정화 효모를 이용한 연속적 에탄올 생산)

  • 한면수;하상도;정동효
    • Microbiology and Biotechnology Letters
    • /
    • v.19 no.4
    • /
    • pp.398-404
    • /
    • 1991
  • - Ethanol production by calcium alginate-immobilized baker's yeast was studied in the continuous shaked-flask reactor (CSFR) using glucose medium as a feed. Immobilized cells were stable at 30~$37^{\circ}C$ and pH 4~8. Fermentation characteristics of immobilized baker's yeast were examined changing the initial glucose concentration employed were 50, 100 and 150 g/l, respectively. It was investigated that the influent glucose concentration and the dilution rate have an influence on the ethanol fermentation characteristics at steady state in continuous culture of immobilized baker's yeast. The optimum conditions for high ethanol productivity and low residual glucose output in ethanol prodution were shown to be 0.2 h ' for the dilution rate and 150 g/l for the influent glucose concentration. The maximum ethanol productivity, ethanol yield, specific growth rate and glucose conversion rate were around 7.12 g/$l\cdot h$, 0.23, 0.366 g/$l\cdot h$ and 78.43, respectively.

  • PDF

Efficiency of Nutrients Removal in a Microbial Contact Oxidation System Covered with Soil (현무암쇄석을 충진한 토양피복형 접촉산화공정의 오염물질제거효율에 관한 연구)

  • Choi, Chul-Ho;Lee, Seung-Mok;Yoon, Jong-Hwa;Hwang, Pil-Gi;Kim, Jung-Yong
    • Journal of Korean Society on Water Environment
    • /
    • v.21 no.1
    • /
    • pp.92-98
    • /
    • 2005
  • Nutrients removal performance of a contact oxidation reactor covered with soil was investigated when basalt rubble was used as a contact medium under various operating conditions. The objective of the experiment was to determine the effectiveness of the system by measuring the removal efficiencies of nutrients from a technical and economical viewpoint. Under the ranges of HRT(20 and 40 hrs) in the experiment, the removal rates of organic matter were as high as 97.5% by showing an effluent $BOD_5$ of less than 10 mg/L. The test of nitrogen removal when the turf was planted on the top soil showed that the average removal rate increased as much as 25% as compared to that without planting. It was suggested that the construction and maintenance cost could be reduced over 20% when the HRT of the system was decreased from 72 to 40 hrs.

Decomposition of volatile aromatic compounds by photochemical treatment (광화학적 방법을 이용한 휘발성 방향족 화합물의 분해)

  • Kim, Jong Hyang
    • Clean Technology
    • /
    • v.4 no.1
    • /
    • pp.35-44
    • /
    • 1998
  • Photodecomposition behaviors of volatile organic compounds (VOCs ; benzene, toluene, ethylbenzene and xylenes) over UV irradiation and UV irradiation with $TiO_2$ powder catalyst were studied and the extent of degradation were also investigated under various reaction conditions. The reactions were conducted in a quartz annular reactor equipped with a medium pressure mercury lamp. As a result, the extents of degradation were 92% for toluene and ethylbenzene, 83% for benzene, and 82% for xylenes under UV irradiation. And they were 92% for toluene, 82% for xylene and ethylbenzene, and 53% for benzene under UV irradiation with $TiO_2$ powder. Analyses of reacted samples by FID-gas chromatograph with Purge & Trap concentrator and GC-MS indicated that the aromatics formed many intermediates.

  • PDF

NUCLEAR HUMAN RESOURCE PROJECTION UP TO 2030 IN KOREA

  • Min, Byung-Joo;Lee, Man-Ki;Nam, Kee-Yung;Jeong, Ki-Ho
    • Nuclear Engineering and Technology
    • /
    • v.43 no.4
    • /
    • pp.375-382
    • /
    • 2011
  • The prospects for growth of the nuclear power industry in Korea have improved remarkably as the demand for energy increases in stride with economic development. Meanwhile, as nuclear energy development is enhanced, nuclear technology has also improved evolutionarily and innovatively in the areas of reactor design and safety measures. As nuclear technology development in Korea advances, more human resources are required. Accordingly, the need for a well-managed program of human resource development (HRD) aimed at assuring needed capacities, skills, and knowledge and maintaining valuable human resources through education and training in various nuclear-related fields has been recognized. A well-defined and object-oriented human resource development and management (HRD&M) is to be developed in order to balance between the dynamics of supply and demand of the workforce in the nuclear industry. The HRD&M schemes include a broad base of disciplines, education, sciences, and technologies within a framework of national sustainable development goals, which are generally considered to include economics, environment, and social concerns. In this study, the projection methodology considering a variety of economic, social, and environmental factors was developed. Using the developed methodology, medium- and long-term nuclear human resources projections up to 2030 were conducted in compliance with the national nuclear technology development programmes and plans.

Nuclear Design Feasibility of the Soluble Boron Free PWR Core

  • Kim, Jong-Chae;Kim, Myung-Hyun;Lee, Un-Chul;Kim, Young-Jin
    • Nuclear Engineering and Technology
    • /
    • v.30 no.4
    • /
    • pp.342-352
    • /
    • 1998
  • A nuclear design feasibility of soluble boron free(SBF core for the medium-sized(600MWe) PWR was investigated. The result conformed that soluble boron free operation could be performed by using current PWR proven technologies. Westinghouse advanced reactor, AP-600 was chosen as a design prototype. Design modification was applied for the assembly design with burnable poison and control rod absorber material. In order to control excess reactivity, large amount of gadolinia integral burnable poison rods were used and B4C was used as a control rod absorber material. For control of bottom shift axial power shape due to high temperature feedback in SBF core, axial zoning of burnable poison was applied to the fuel assemblies design. The combination of enrichment and rod number zoning for burnable poison could make an excess reactivity swing flat within around 1% and these also led effective control on axial power offset and peak pin power, The safety assessment of the designed core was peformed by the calculation of MTC, FTC and shutdown margin. MTC in designed SBF core was greater around 6 times than one of Ulchin unit 3&4. Utilization of enriched BIO(up to 50w1o) in B4C shutdown control rods provided enough shutdown margin as well as subcriticality at cold refueling condition.

  • PDF

Enzymatic Production of Structured Lipids from Capric Acid and Conjugated Linoleic Acid in Soybean Oil

  • Shin, Jung-Ah;Lee, Ki-Teak
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
    • /
    • 2003.10a
    • /
    • pp.164.1-164
    • /
    • 2003
  • In this study, medium-chain fatty acid (MCFA) metabolized in the liver for quick energy and CLA exhibited biological activity were used for synthesis of structured lipids (SLs). SLs were synthesized by acidolysis of soybean oil, capric acid (C10:0) and CLA with Chirazyme L-2 lipase as biocatalysts. The effect of enzyme load (2, 4, 6, 8, 10% w/w substrates) was investigated. Production of SL (scale-up) was performed with a 1:2:2 molar ratio (oi1/C10:0/CLA) for 24 h at 55$^{\circ}C$ in a stirred batch reactor (420 rpm). The reaction was catalyzed by Chirazyme L-2 lipase (24.48g, 4% w/w substrates). The scale-up result showed that capric acid and total CLA were incorporated 4.9%, 4.1% (mole%), respectively, in soybean oil. Then, physio-chemical property and flavor characteristic of produced SL-soybean oil were analyzed. Therefore, SL-soybean oil containing C10:0 and CLA was successfully synthesized and may be beneficial in desirable food and nutritional applications.

  • PDF

Experimental Study on Design Verification of New Concept for Integral Reactor Safety System (일체형원자로의 신개념 안전계통 실증을 위한 실험적 연구)

  • Chung, Moon-Ki;Choi, Ki-Yong;Park, Hyun-Sik;Cho, Seok;Park, Choon-Kyung;Lee, Sung-Jae;Song, Chul-Hwa
    • Proceedings of the KSME Conference
    • /
    • 2004.04a
    • /
    • pp.2053-2058
    • /
    • 2004
  • The pressurized light water cooled, medium power (330 MWt) SMART (System-integrated Modular Advanced ReacTor) has been under development at KAERI for a dual purpose : seawater desalination and electricity generation. The SMART design verification phase was followed to conduct various separate effects tests and comprehensive integral effect tests. The high temperature / high pressure thermal-hydraulic test facility, VISTA(Experimental Verification by Integral Simulation of Transient and Accidents) has been constructed to simulate the SMART-P (the one fifth scaled pilot plant) by KAERI. Experimental tests have been performed to investigate the thermal-hydraulic dynamic characteristics of the primary and the secondary systems. Heat transfer characteristics and natural circulation performance of the PRHRS (Passive Residual Heat Removal System) of SMART-P were also investigated using the VISTA facility. The coolant flows steadily in the natural circulation loop which is composed of the steam generator (SG) primary side, the secondary system, and the PRHRS. The heat transfers through the PRHRS heat exchanger and ECT are sufficient enough to enable the natural circulation of the coolant.

  • PDF