• KSBB Journal
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• 제8권5호
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• pp.483-487
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• 1993

기존의 무혈청 배지에 첨가하던 BSA/acid를 acid/Pluronic F-68 emulsion으로 대치하여도 세포성장에는 전혀 지장이 없었다. BSA를 제외함으로써 무혈청 배지에 첨가하는 단백질 함량을 최소하할 수 있었고, 이로 인한 세포농도 감소는 없었다. SFLSM은 0.2micron 필터로 여과 멸균하여도 SFLSM의 세포성장효과는 영향을 받지 않는다.

• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.208-213
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• 1998

The effect of the addition of wheat bran to the growth medium on the production of cellulolytic enzymes of Trichoderma reesei Rut C30 was studied in batch culture using shake flasks. The activity of cellulase was enhanced by the addition of wheat bran to the cellulase production medium. $KH_2PO_4$-$K_2HPO_4$ buffer was used for pH control during cellulase production. As a result, high cellulase activities were obtained in shake flask culture; a CMC (carboxymethyl cellulose) activity of 125.78 U/ml was obtained from 2% Avicel- and 3% wheat bran-containing medium and an FP (filter paper) activity of 12.85U/ml was obtained from 1% Avicel- and 5% wheat bran-containing medium after 6 days of cultivation.

• 미생물학회지
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• 제52권1호
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• pp.25-31
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• 2016

분변오염의 지표가 되는 장구균을 검출하기 위하여 장구균 선택 배지를 개발하였다. 기존에 알려진 여러 장구균 검출배지 중 총 9종류(Enterococci Confirmatory agar, Azide dextrose agar, Bromocresol-purple azide agar, Esculin bile agar, Citrate azide tween carbonate agar, KF Streptococcus agar, BROLACIN agar, Kanamycin esculin azide agar, Membrane filter Enterococcus selective agar)의 배지를 선별하였고, 배지 성분을 적절히 조합하여 새로운 배지 Enterococcus Mixed medium을 개발하였다. 배지의 효과를 검증하기 위하여 장구균 6종류(Enterococcus avium KACC 10788, Enterococcus faecium KACC 11954, Enterococcus saccharolyticus KACC 10783, Enterococcus durans KACC 10787, Enterococcus faecalis KACC 11304, Enterococcus hirae KACC 10779)와 비장구균 3종류(Escherichia coli KACC 10005, Staphylococcus aureus subsp. aureus KACC 10768, Bacillus subtilis KACC 10111)를 사용하였다. 그 결과 새롭게 개발된 장구균 검출배지에서 비장구균의 생장이 억제된 반면에 장구균 6종류는 모두 생장하는 것을 확인하였다. 또 환경시료에서 장구균을 분리할 수 있는지 확인하기 위해 하천과 바다에서 채취해온 시료를 이용하여 실험한 결과 장구균의 생장을 확인하였다. 따라서 이 배지를 이용하면 수질 분변오염의 지표가 되는 장구균을 효과적으로 검출할 수 있음을 확인하였다.

• 한국안광학회지
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• 제5권1호
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• pp.83-87
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• 2000

본 연구는 각막상피가 저절로 탈락해 나가는 과정을 이해하기 위해 사람의 각막상피 세포를 배양하여 배지의 영양이 고갈될 때까지 약 7일 정도 배지를 교환하지 않고 계속 배양하여 세포고사를 조사하였다. 영양이 고갈된 배지인 Exhausted Medium을 여과하여 새로운 각막 상피세포에 넣어 2일 정도 배양을 계속하였다. Exhausted Medium에서 배양된 세포를 수확하여 세포고사를 검정하기 위해 Agarose gel electrophoresis로 DNA 단편을 확인하고 세포고사를 초기에 감지할 수 있는 방법인 M30 $CytoDEATH^*$. Fluorescein으로 확인하였다. 또한 Exhausted Medium에 의한 세포고사의 유발 경로를 조사하기 위해 사이토카인에 의한 유도인자 중 가장 많이 알려진 FAS나 FAS Ligand에 의한 유발여부를 soluble FAS and FAS Ligand에 대한 sandwich ELISA로 조사하였다. 그 결과 전형적인 DNA Ladder패턴을 보였으며 M30 $CytoDEATH^*$. Fluorescein에도 선명하게 염색되어 세포고사를 확인할 수 있었다. 또한 soluble FAS and FAS Ligand ELISA Kit로 Exhausted Medium이 FAS와 관련이 있는지를 조사한 결과 거의 무관한 것으로 나타났다. 본 연구의 결과는 영양 부족상태의 배지는 각막상피 세포가 세포고사를 거쳐 소모되는데 FAS and FAS Ligand system과는 무관한 것으로 나타났다.

• 한국산업보건학회지
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• 제19권1호
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• pp.1-7
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• 2009

To establish the Fourier-Transform Infra-Red spectrophotometry(FTIR) Direct-On-Filter(DOF) technique as a useful analytical method for quartz in respirable dust samples, an influence of the kaolinite should be corrected. Respirable dust, created in a dust chamber containing the standard material of quartz and kaolinite were collected using a cyclone equipped with a 25 mm, $0.8\;{\mu}m$ pore size DM filter as a collection medium. This study was designed to compare three methods of correction for kaolinite when quantifying the content of quartz, including the least square, the optimum choice and the spectral subtraction methods. The content of quartz in the respirable dust samples was overestimated by 6.2% when mixed with kaolinite(35.5% by weight). The content of quartz containing kaolinite(72.8% by weight) were overestimated by 32%. The spectral subtraction method underestimated the quartz content by 1.5%, while the other two correction methods, the optimum choice and the least square method, overestimated the quartz content by 1.9% to 6.4% and 0.04 to 1.1%, respectively. The results of this study are suggested that, when correcting for effects of kaolinite on quantitative analysis of quartz in respirable dust by FTIR direct-on-filter method, the least square method produce the most unbiased results be compared with those of other correction methods.

• 제어로봇시스템학회논문지
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• 제22권2호
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• pp.133-138
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• 2016

This paper describes the track initiation and target-tracking filter for ship tracking in a marine environment by using Light Detection And Ranging (LiDAR). LiDAR with three-dimensional scanning capability is more useful for target tracking in the short to medium range compared to RADAR. LiDAR has rotating multi-beams that return point clouds reflected from targets. Through preprocessing the cluster of the point cloud, the center point can be obtained from the cloud. Target tracking is carried out by using the center points of targets. The track of the target is initiated by investigating the normalized distance between the center points and connecting the points. The regular track obtained from the track initiation can be maintained by the target-tracking filter, which is commonly used in radar target tracking. The target-tracking filter is constructed to track a maneuvering target in a cluttered environment. The target-tracking algorithm including track initiation is experimentally evaluated in a sea-trial test with several boats.

• 환경위생공학
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• 제24권3호
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• pp.16-27
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• 2009

In this paper, I examined the level of fine dust in medical institutions, educational institutions and multi-purposed facilities to grasp the exact state of the present, and decided the level of air-borne particulate(KSM ISO Standard and ISO Standard 14644-1). We compared new proposed cleaner equipped with HEPA Filter with general cleaner and analyzed the rate of removal according to height, air volume and the equipment with the compulsive air intake. Through this comparison, I reached the conclusion as follows: 1. According to the examination, the fine dust of medical institutions, educational institutions and multi-purposed facilities in Kwang Ju is class 9. 2. The filter used in general cleaner on the market is that of HEPA-type, and its removal efficiency for fine particles($0.3{\sim}0.5{\mu}m$) is very low. 3. In the removal efficiency of new proposed cleaner equipped with HEPA Filter, the higher it is, the better, especially more than 180cm in height. 4. In case it is operated for 5 minutes under the condition of the space of $9.4m^{3}$ and the maximum air volume equipped with two induction pipes, we can keep the air cleanness level of 5 ~ 6. 5. To maintain the air cleanness for a long time, if we first operate for 5 minutes at maximum air volume and then operate at medium maximum air volume, we can keep the air cleanness with low energy.

• ETRI Journal
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• 제38권2호
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• pp.356-365
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• 2016

This research proposes a modified particle filter to increase the accuracy of vehicle tracking in a noisy and occluded medium. In our proposed method for vehicle tracking, the direction angle of a target vehicle is calculated. The angular difference between the motion direction of the target vehicle and each particle of the particle filter is observed. Particles are filtered and weighted depending on their angular distance to the motion direction. Those particles moving in a direction similar to that of the target vehicle are assigned larger weights; this, in turn, increases their probability in a given likelihood function (part of the process of estimation of a target's state parameters). The proposed method is compared against a condensation algorithm. Our results show that the proposed method improves the stability of a particle filter tracker and decreases the particle consumption.

• 수산해양기술연구
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• 제54권1호
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• pp.73-80
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• 2018

Intermittent duty of emergency generator has problems emitting large quantities of PM and NOx in exhaust gas. The aim of this study is to propose DPF system which can be applied to medium-large emergency generators. The system is composed of soot dust collector, silencer and filter trap, which is designed to reduce PM emissions at the emergency generator start-up. The pneumatic system controls a flow direction of exhaust gas to pass through the soot collector and filter trap until the engine reaches complete combustion condition. An experiment is performed to measure PM content and concentration to analyze the performance and characteristics of the proposed system.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2006년도 하계학술발표대회 논문집
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• pp.1131-1136
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• 2006

In this study, an experimental and numerical analysis on the flow characteristics of heating and cooling unit installed within ceiling with functional modules was carried out. And the flow characteristics of the heating and cooling unit was investigated with the main parameters such as each filter and inside structure. The experimental results show the possibility of medium filter application. And the numerical results show that the effect of improved inside structure of heating-cooling and air-conditioning unit on flow distribution.