• KSBB Journal
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• v.16 no.6
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• pp.603-608
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• 2001

In this study, we studied on the remeval of toluene vapors in a lab-scale biofilter. Biofiltration was performed in a column fed downflow with contaminated air at ambient conditions. The column was packed with mixture of Peat and Calstene(5:3 vol. Ratio), Synthesized media, Bark and Wood chip, which were inoculated with microbial population of selected stains(Pseudomonas. putida, KCCM 11343, ATCC 12633). The microorganisms were immobilized on the bed medium and then biofilm were formed. The biofilter was operated under the conditions of various inlet toluene concentrations for 180 days and treated up to the elimination capacity of maximum 40 g/㎥hr at the inlet load of 30 g/㎥ hr with percentage removals of 20∼90% and gas retention times between 1 and 2 min. The pressure drop was very negligible through the biofilter columps because its value of 0.054 cmH$_2$O/m was much less than others. The effect of operating conditions such as flow rate, inlet toluene concentration and moisture contents on the performance of the biofilter was sequentially investigated in this study.

• Korean Journal of Microbiology
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• v.43 no.2
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• pp.106-110
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• 2007

The detection and distribution of Aeromonas in water supplies were investigated by using the USEPA Method 1605. Water samples were collected from the Han River, finished waters and tap waters supplied from Water Treatment Plants in Seoul monthly from July 2002 to December 2003. Aeromonas species in each water sample were quantified based on the development of yellow colonies on the surface of membrane filter using a selective medium (Ampicillin-Dextrin Agar with Vancomycin). The Quality Control (QC) for this study met the acceptance criteria of Method 1605. The concentrations of Aeromonas species in surface water samples ranged from $1.0{\times}10^{0}\;to\;9.8{\times}10^{3}\;CFU/ml$. Aeromonas species were found only in one tap water sample with concentration of 1 CFU/500 ml. No Aeromonas species were found in any finished water samples. Aeromonas species detected here were identified as A. salmonicida(51%), A. caviae(4.7%), A. schubertti(3.4%), A. sobria(3.8%), A. hydrophila(2.1%), and A. ichithiosmia(0.4%). A. salmonicida was the dominant species, which is of no significance to human health. Chlorine resistance of A. salmonicida was evaluated and as a result, 99.99% of A. salmonicida decreased after 30 seconds exposure at residual free chlorine 0.2 mg/L. These suggest that the waters supplied in Seoul may be safe against the pathogenic agent Aeromonas.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.852-858
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• 1998

We have isolated a bacterial strain that tends to kill P. micans from the mixed culture of p. minns plus seawater filtrate (poresize, 0.8 $\mu$m) collected at Masan bay in July 1996, in which the mixed culture grown in the f/2 medium. According to the experimental results of the isolated bacterium such as fatty acids analysis, morphological and biochemical characteristic tests, the strain was supposed to be a Pseudomonas and then it was named as Pseudomonas sp. LG-2. The killing effect of Pseudomonas sp. LG-2 against P. micans was proportionally increased with the concentrations of culture filtrate (pore size, 0.8 $\mu$m) is well as with the number of bacterium inoculated. In the mixed culture inoculated with $1.3\times10^6$ cells/ml of Pseudomonas sp. LG-2, the number of P. micans (2,000 cells/ml) was gradually decreased and then killed below 100 cells/ml within 7 days. In addition, the culture filtrate with $30\%$ of final concentration revealed a significant killing effect against P. micans around 3 days after culture. In the relationship between killing effects and growth stage of Pseudomonas sp. LG-2, the culture filtrate at lag phase has little effects on P. micans. In constant, the culture filtrate at mid-log phase showed the killing effect by decreasing P. micans to 112 in number within 5 days. In particular, the culture filtrate at stationary phase showed a significant killing effect against P. micans in which the majority of it was killed after 3 day culture. The species specificity of killing effects of Pseudomonas sp. LG-2 against 5 species of dinoflagellate was only found in P. micans and Scrippsiella trochoidea.

• The Journal of Internal Korean Medicine
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• v.31 no.2
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• pp.189-200
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• 2010

Objectives : Regulatory T cells can reduce inflammation and allergic reactions through their inhibitory functions. Gardeniae Fructus(GF) is a Heat-clearing herb used in traditional Korean medicine, and a wide range of studies on its antiinflammatory effects are being carried out. The authors investigated the effect that Gardeniae Fructus has on regulatory T cells. Methods : The authors screened 14 herbs for their effects on regulatory T cells. 100mg of each herb were separately dissolved in 1ml of sterile saline and the supernatant was harvested after 10 minutes of centrifuge at 15,000 rpm. The supernatant was filtered through a 0.2 ${\mu}m$ syringe filter, and the resulting stock was refrigerated at $4^{\circ}C$. The stock was diluted before testing and used at a final concentration of $0.01{\mu}g/ml$. CD4+CD25+ T cells from healthy BALB/c spleens were used as natural regulatory T cells (nTreg), and CD4+CD25- T cells were used as reactive T cells. CD4+CD25+ and CD4+CD25- T cells were activated with anti-CD3e ($10{\mu}g/m{\ell}$)/anti-CD28 ($1{\mu}g/m{\ell}$) and cultured. IL-10 from supernatant of the culture medium was measured by IL-10 cytokine ELISA. The percentages, cell numbers, phenotype and function of CD4+CD25+ Treg cells were determined by flow cytometry. Results : Gardeniae Fructus was shown to be the most potent herb among the 14 herbs tested for suppressing CD4+CD25- reactive T cell proliferation by stimulating CD4+CD25+ natural regulatory T cells. Gardeniae Fructus induces IL-10 secretion increase by stimulating CD4+CD25+ natural regulatory T cells, and indirectly suppresses CD4+CD25- reactive T cell proliferation through increasing CD25 (IL-2 receptor $\alpha$) expression and thus promoting bonding with IL-2. Gardeniae Fructus did not directly affect CD4+CD25- reactive T cell proliferation. Conclusions : Gardeniae Fructus suppressed reactive T cell proliferation through inducing increases in IL-10 secretion and CD25 (IL-2 receptor $\alpha$) expression.

• Journal of Korean Society on Water Environment
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• v.20 no.5
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• pp.500-505
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• 2004

While more attention has been paid in recent years to urban point source pollution control, no considerable measures have been taken to control urban non-point source pollution. Contaminants from non-point sources deposited on urban impermeable surfaces such as road and highway are easily released to the public waters by rainfall, leading to the deterioration in water quality in urban area. The present study investigated a system using filter media to reduce the level of contamination in urban road runoff. Three different media, designated as J-P (polypropylene, blowing type), B-P (polypropylene, fiber type), and P-F (Polyvinyl alcohol, cube and sheet type) were evaluated on the basis of pollutant removal efficiency as well as hydraulic property. Experiments were peformed using a 1/4-scale treatment unit at various pollutant roading and rainfall intensity with real rainwater runoff collected from road surfaces. The results at different rainfall intensities shows that the medium J-P is superior to the other media for water permeability. J-P was not blocked at 10 mm/hr rainfall and its permeability was stable. On the other hand, B-P and P-F resulted in substantial overflow ratio even at I mm/hr rainfall intensity. Comparing treatment efficiencies for suspended solids, all media showed similar removal ranged from 91 % to 95%. However, J-P seems to be better than the other media considering its higher water permeability. J-P and P-F, both hydrophobic, yielded over 76% of heavy metal removal. But, the hydrophilic media B-P showed much smaller removal ranging from 26% to 47%. This indicates that J-P and P-F are beneficial to remove heavy metal due to their hydrophobic property. The treatment efficiencies over PAHs (poly aromatic hydrocarbons) showed the similar removal efficiency ranging from 66% to 97% for all three media.

• Journal of the Korean association of regional geographers
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• v.2 no.2
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• pp.197-204
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• 1996

We attempt to classifing method of micro-landform on the alluvial plain, such as natural-levee, backmarsh and alluvial fan, using false color composite of Landsat Thematic Mapper image. The study area is Kumho River Basin on the southeastern part of Korea peninsula. The most effective image for micro-landform classification is the false color composite of band 2, 3 and 4 with blue, green and red filtering. The most favorable time is the middle third of November, because of the density differentiation of green vegetation in most great. In this time the paddy field on the back-marsh is bare by rice harvesting. But on the natural levee the green vegetation, such as vegetables and lower herbs under fruit tree, remain relatively more. On the alluvial fan, the green vegetation condition is medium. For the verification of the micro-landform classification, we employed the field survey and grain size analysis of the deposition of each micro-landform on the sample area. It is clarified that the classification method of micro-landform on the alluvial plain using the Landsat TM image is relatively useful.

• Korean Journal of Food Science and Technology
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• v.43 no.3
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• pp.348-360
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• 2011

To verify the volatile compounds contributing to coffee off-flavor, medium roasted Colombian, Sumatra, Ethiopian and light and dark roasted Colombian bean coffees were sequentially and studiously over extracted. Eight espresso samples and eight filter-dripped samples were analyzed by gas chromatography/mass spectrometry with the solidphase microextraction method and evaluated by sensory tests. In total, 67 compounds were detected, and desirable aroma decreased sharply, while off-flavors increased rapidly after 30 mL of espresso and 200 mL of drip coffee. Percent peak area of 4-ethylguaiacol revealed a linear increase during extraction and was highly correlated with increasing off-flavor, suggesting that it could be an indicator of over extraction. Considering the odor activity value, guaiacol and 4- vinylguaiacol were also contributory compounds to off-flavors.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.11
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• pp.3121-3129
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• 2009

Conventional marine radar systems utilize pulse radar which is capable of high-power transmissions and is effective for remote detection purposes. A pulse radar is most commonly used on medium or large vessels due to its expensive installation and maintenance costs. I propose the use of a Frequency Modulated Continuous Wave (FMCW) radar system operated at low-power and high-resolution instead of the conventional pulse-radar based system. The transmitted and received signals of the FMCW radar system were theoretically analyzed and radar signal processing design and simulation experiments were performed to detect the range and speed. Intermediate Frequency (IF) signal mixed with virtual transmit and receive signals were generated to perform FMCW radar signal processing simulations where the IF signal underwent noise reduction through a lowpass filter. The maximum frequency was derived through the sample interval of the FFT size instead of using A/D converter. This maximum frequency was used to get the frequency range and frequency speed which were in turn used to calculate the range and speed. The virtual beat frequency generated using MATLAB is utilized to analyze the beat frequency used in the actual FMCW radar system signal processing. The differences in the range and speed of the beat frequency signals are processed and analyzed.

• Journal of Life Science
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• v.21 no.11
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• pp.1579-1585
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• 2011

Natural anti-diabetic semipurified ethyl acetate fraction was isolated from the submerged-liquid culture of Agaricus blaze Murill (AB) in a medium containing soybean flakes. Hot-water extract of AB (HEAB) was prepared by extraction at $121^{\circ}C$ for 60 min, followed by filtering through a filter presser filled with diatomate. The ${\beta}$-glucan-free HEAB, which was a supernatant fraction from HEAB by precipitation in an 80% ethanol solution, was fractionated into hexane, chloroform, ethyl acetate, and butanol fractions. The inhibition of the ${\alpha}$-glucosidase activity by fractions was 59.0, 17.0, 61.6, and 37.9%, respectively, suggesting that ethyl acetate fraction was the most active. A subfraction having a strong ${\alpha}$-glucosidase inhibitory activity (80.4%) was isolated from the ethyl acetate fraction. This subfraction contained isoflavones (genistin and daidzin) and their conjugates with sugars as potent inhibiters of ${\alpha}$-glucosidase activity. These results suggest that the ethyl acetate fraction or HEAB containing isoflavones and their sugars conjugates could be useful sources for controlling blood sugar levels in humans.

• Progress in Medical Physics
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• v.14 no.2
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• pp.81-89
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• 2003

The dose distributions of designed Ir-192 micro-source were investigated by dose computations which were accomplished by employing shape of encapsule material and thickness of the source for self-absorption. The computation dose derived from air-kerma rate (S$_{k}$ ) and dose rate constant (Λ) includes the anisotropy of dose distribution around the source. We got the dose rate constants in a water medium is 1.154 cGy h$^{-1}$ U$^{-1}$ . The size of the source was 0.5 mm in diameter and 3.5 mm in length and it was encapsuled in 1.1 mm$\Phi$${\times}$5.5 mm of stainless steel sealed with 0.3 mm of filter thickness. The tissue dose of reference point at 1.0 cm radial distance of the source axis was delivered 1.154 Uh$^{-1}$ (1.3167${\times}$10$^{-3}$ cGy/mCi-sec) from the S$_{k}$ 4.108U/mCi of Ir-192 source. The filtration effect contributed to air-kerma strength as exponential filtering effect of 86.2% in total attenuation, but self-absorption was 88.4% from radial dose distributions. In particular, the dose attenuations showed a rapid anisotropic distributions as 56% of reference dose along to $\pm$10 degrees from the tip of source axis and 50% for of that to source-cable direction. We persist in use the large diameter of applicator will avoid the dose anisotropy by the filtered attenuation effects along the axis of Ir-192 micro-source.