• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.75-75
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• 2018

본 연구는 발효 옻나무와 구절초를 복합소재로 활용하여 화장품 소재로서의 개발 가능성을 확인하고자 항산화, 항주름 및 미백활성을 검증하였다. 구절초와 발효옻나무를 각각 및 각 비율별로 혼합하여 항산화 활성, 항주름 활성, 미백활성을 확인하였다. 구절초와 발효옻나무의 최적 혼합비율을 설정하기 위해 다양한 혼합비에서 선별하였으며, 구절초 1: 옻나무 9의 활성이 가장 효과적이었다. 혼합물의 DPPH, ABTS 라디칼 소거활성은 $1,000{\mu}g/ml$에서 각각 $95.78{\pm}3.24%$, $99.01{\pm}1.80%$로 나타났고, reducing power은 $1,000{\mu}g/ml$에서 $81.48{\pm}1.47%$로 확인되었다. Tyrosinase 저해활성은 $1,000{\mu}g/ml$에서 $16.74{\pm}1.85%$로, Collagenase 저해활성은 $20{\mu}g/ml$에서 $112.40{\pm}7.75%$로 나타났다. 미백 활성을 확인하기 위한 대조군으로 arbutin을 사용하였으며, B16 F10 세포를 통한 미백 활성은 혼합물 처리에 의해 tyrosinase, TRP-1, TRP-2, MITF protein 및 mRNA 발현을 유의성 있게 저해하였으며, arbutin과 유사한 효과를 나타냈다. 또한 wound healing assay를 통한 피부 장벽 손실 억제 효과를 확인하였다. 따라서 본 연구에서는 구절초와 발효옻나무의 혼합물의 높은 항산화, 항주름 및 미백활성을 통해 천연화장품의 소재로서의 높은 가치를 확인하였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.76-76
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• 2018

화학적 살충제는 빠르고 정확하게 해충을 방제하는 효과를 나타내지만, 인간과 다른 유기체에 대한 살충제 잔류의 위험성, 화학적 살충에 대한 저항성을 가진 해충의 출현, 생태계의 교란 등 다양한 부작용이 있다. 이 때문에 생물적 방제를 통한 해충 방제는 효과적인 수단이 될 수 있다. 특히 채소 작물 등에 주로 발생하는 응애는 생산량 저하 및 품질 저하 등과 같은 다양한 문제를 일으킬 수 있다. 특히 진딧물이나 점박이응애는 화학적 살충제에 대해 높은 저항성이 발달되어 문제가 되고 있다. 본 연구에서는 아이비(Hedera helix), 란타나(Lantana camara), 예루살렘체리(Solanum pseudocapsicum)를 통한 천연 식물 추출물의 응애에 대한 생물적 방제 효과를 확인하였다. 각 추출물은 0.5%, 0.05% 주정 및 메탄올로 추출하였으며, 응애 방제 효과(%)는 아이비>란타나>예루살렘체리로 나타났다. 각 추출물은 GC/MS 분석을 통해 생리활성 후보 물질을 확인하였다. 이를 통해 농업 이익 증대, 생산량 유지와 더불어 환경 및 인간과 다른 유기체에 피해를 주지 않으며 효과적으로 응애를 방제 할 수 있는 천연 생물적 방제제로의 가치를 확인하였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.88-88
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• 2018

Coffee is one of the most popular drinks in the world. Roasting process of coffee bean is one of major steps to make coffee, however, there are few studies which analyzed chemical compounds in intermediate state of roasting coffee beans due to technical limitations to get coffee beans with the same roasting condition. We utilized Stronghold S7 pro roasting machine which guarantees the saming roasting conditions repletively with the aid of precise computer to control heat sources to get 20 steps (every 30 seconds) of roasted coffee beans during roasting process (10 min in total). Along with roasting process, phenolic compounds were decreased, which can be explained that roasting process cause phenolic compounds degradation. Caffeine is almost constant during roasting, reflecting that caffeine is not affected in roasting process. These samples presents that organic acids significantly increase along with the roasting process by HPLC analysis. With additional analysis of coffee beans, such as moisture contents, pH, as well as coffee tastes, our analysis will show detailed process of chemical compounds of coffee beans during roasting process.

• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.578-585
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• 2020

This study was designed to assess the possibility of using medicinal plant extracts as β-lactamase inhibitors to control antibiotic-resistant Staphylococcus aureus. The susceptibilities of S. aureus ATCC 15564 (SA^WT), ciprofloxacininduced S. aureus ATCC 15564 (SA^CIP), oxacillin-induced S. aureus ATCC 15564 (SA^OXA), and clinically-isolated S. aureus CCARM 3008 (SA^CLI) to ampicillin were determined in the absence and presence of medicinal plant extracts, including Cleyera japonica (CJ), Carpinus laxiflora (CL), Euphorbia helioscopia (EH), Euscaphis japonica (EJ), Oenothera erythrosepala (OE), and Rosa multiflora (RM). The phenotypic change in the clear inhibition zones around ampicillin disc was observed for SA^WT, SA^CIP, and SA^OXA, indicating the production of ampicillinase. Compared to the controls, the MICs of ampicillin against SA^WT, SA^CIP, and SA^OXA were decreased from 4 to 0.5 ㎍/mL in the presence of CL, 16 to 4 ㎍/mL in the presence of RM, and 32 to 2 ㎍/mL in the presence of CL, EH, and RM, respectively. The medicinal plant extracts, OE, EJ, and CL, effectively inhibited the β-lactamase activities of SA^WT (78%), SA^CIP (57%), and SA^OXA (76%) when compared to the control. This results suggest that the medicinal plant extracts can be used as BLIs to control the antibiotic-resistant S. aureus.

• Korean Journal of Plant Taxonomy
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• v.51 no.2
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• pp.115-132
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• 2021

Seven species and two varieties belonging to the genus Boehmeria Jacq. (Urticaceae) are known to be distributed in Korea. Among them, B. spicata, B. tricuspis, and B. tricuspis var. unicuspis were subjected to an external morphological study. Among the individuals believed to exhibit variations in the leaf shape, B. gracilis and B. silvestrii were newly recognized. Unlike related taxa, B. gracilis has middle leaves with an elliptic, broadly elliptic, ovate, or broadly depressed ovate shape, a regular and serrulate-dentate margin, and an unlobed and short caudate or cuspidate apex. Boehmeria silvestrii has middle leaves with 5-angled ovate, orbicular ovate or broadly ovate shapes, and 3- or 5-lobed and caudate apices. Therefore, we assigned the corresponding names 'Top-geo-buk-kko-ri' and 'Cham-geo-buk-kko-ri'. Meanwhile, the B. spicata complex (B. gracilis, B. silvestrii, B. spicata, and B. tricuspis var. unicuspis) is very closely related in terms of the morphological characters, whereas B. tricuspis exhibits no relationship. Furthermore, because the scientific name and type specimen of B. tricuspis var. unicuspis (Pul-geo-buk-kko-ri) are problematic, the correct name B. paraspicata Nakai and corresponding holotype are presented. Additionally, lectotypes of B. gracilis and B. silvestrii are newly designated here. A taxonomic treatment, descriptions, a key, photographs, type specimens, and leaf variation images of the B. spicata complex are provided in this study.

• Korean Journal of Plant Resources
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• v.35 no.2
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• pp.385-389
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• 2022

In this study, we measured the inhibitory activity of Pinus densiflora leaf (PDL) against excessive lipid accumulation in mouse preadipocyte, 3T3-L1 cells to investigate whether PDL exerts anti-obesity activity. Lipid accumulation and the protein level were measured using Oil red O staining assay and Western blot analysis, respectively. We observed that PDL inhibited excessive lipid accumulation and decreased the expression of CEBPα, PPARγ and perilipin-1 related to lipid accumulation in 3T3-L1 cells. Therefore, considering these results, PDL can be used as a potential agent for anti-obesity.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.44-44
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• 2005

• Korean Journal of Plant Resources
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• v.31 no.3
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• pp.218-227
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• 2018

In this study, we investigated the effect of the extracts from Vaccinium oldhamii on cell proliferation and the regulatory mechanisms of cyclin D1 protein level in human cancer cells. The branch extracts from Vaccinium oldhamii (VOB) showed higher inhibitor effect against the cell growth than leave extracts (VOL) and fruit extracts (VOF) in human colorectal cancer, breast cancer, prostate cancer, non-small lung cancer, pancreatic cancer and liver cancer cells. In addition, VOB decreased cyclin D1 level at both protein and mRNA level. MG132 treatment attenuated VOB-mediated cyclin D1 downregulation. A point mutation of threonine-286 to alanine attenuated cyclin D1 degradation by VOB. In addition, the inhibition of nuclear export by leptomycin B (LMB) attenuated cyclin D1 degradation by VOB. But, the treatment of PD98059 (ERK1/2 inhibitor), SB203580 (p38 inhibitor), SP600125 (JNK inhibitor), LiCl ($GSK3{\beta}$ inhibitor), LY294002 (PI3K inhibitor) or BAY 11-7082 ($I{\kappa}K$ inhibitor) did not affect VOB-induced cyclin D1 degradation. In conclusion, VOB induced cyclin D1 degradation through redistribution of cyclin D1 from the nucleus to cytoplasm via T286 phosphorylation of cyclin D1, which resulted in the inhibition of cancer cell proliferation.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.9
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• pp.1289-1294
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• 2013

This study evaluated the in vitro effect of medicinal plant extracts on ruminal methanogenesis, four different groups of methanogens and ruminal fermentation characteristics. A fistulated Holstein cow was used as a donor of rumen fluid. Licorice and mugwort extracts (Glycyrrhiza uralensis and Artemisia capillaris, 0.5% and 1% of total substrate DM, respectively), previously used as folk remedies, were added to an in vitro fermentation incubated with buffered-rumen fluid. Total gas production in Glycyrrhiza uralensis extract treatment was not significantly different between treatments (p<0.05) while total gas production in the Artemisia capillaris extract treatment was lower than that of the control. Artemisia capillaris extract and Glycyrrhiza uralensis extract reduced $CH_4$ emission by 14% (p<0.05) and 8% (p<0.05), respectively. Ciliate-associated methanogens population decreased by 18% in the medicinal plant extracts treatments. Medicinal plant extracts also affected the order Methanobacteriales community. Methanobacteriales diversity decreased by 35% in the Glycyrrhiza uralensis extract treatment and 30% in the Artemisia capillaris extract treatment. The order Methanomicrobiales population decreased by 50% in the 0.5% of Glycyrrhiza uralensis extract treatment. These findings demonstrate that medicinal plant extracts have the potential to inhibit in vitro ruminal methanogenesis.

• The Korea Journal of Herbology
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• v.31 no.5
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• pp.55-62
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• 2016

Objective : TM-MC was constructed to provide information on the chemical compounds of medicinal plant materials which were listed in Korean, Chinese, and Japanese pharmacopoeias. The aim of this study was to compare of the chemical compounds of TM-MC with those of domestic pharmacopoeias.Methods : First of all, TM-MC was updated on medicinal materials and chemical compounds which were extracted from articles up to the PubMed identifier of 26 millions. After searching for medicinal plant materials with information on their major compounds in Korean pharmacopoeias, we identified that the information on medicinal materials and chemical compounds were also existed in TM-MC. Then, we figured out how much the compounds have been studied by counting the number of articles for those compounds in TM-MC.Results : Information on 9 medicinal materials and about 1,700 compounds was newly added to TM-MC after manually curating 645 articles. Korean pharmacopoeias contained a total of 91compounds from 53 medicinal plant materials, while TM-MC provided information on the 89 compounds from the 53 medicinal materials in Korean pharmacopoeias. The compounds stated in Korean pharmacopoeias has been much studied by comparison with other compounds of each medicinal material in TM-MC.Conclusion : Most of chemical compounds stated in Korean pharmacopoeias were also listed in TM-MC, excluding two compounds. Thus, we concluded that information in TM-MC is mostly consistent with that of Korean pharmacopoeias. In the future, we will periodically update TM-MC with articles added to the MEDLINE.