• BMB Reports
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• 제47권4호
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• pp.221-226
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• 2014

Drug-resistance and imbalance of apoptotic regulation limit chemotherapy clinical application for the human hepatocellular carcinoma (HCC) treatment. The reactivation of p53 is an attractive therapeutic strategy in cancer with disrupted-p53 function. Nutlin-3, a MDM2 antagonist, has antitumor activity in various cancers. The post-translational modifications of p53 are a hot topic, but there are some controversy ideas about the function of phospho-$Ser^{392}$-p53 protein in cancer cell lines in response to Nutlin-3. Therefore, we investigated the relationship between Nutlin-3 and phospho-$Ser^{392}$-p53 protein expression levels in SMMC-7721 (wild-type TP53) and HuH-7 cells (mutant TP53). We demonstrated that Nutlin-3 induced apoptosis through down-regulation phospho-$Ser^{392}$-p53 in two HCC cells. The result suggests that inhibition of p53 phosphorylation on $Ser^{392}$ presents an alternative for HCC chemotherapy.

• 한국화재소방학회논문지
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• 제27권6호
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• pp.122-128
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• 2013

본 연구는 2010년 1월 1일부터 2011년 12월 31일까지 2년간 119구급대로 이송된 병원 전 제세동 가능한 심정지 환자를 대상으로 연구를 진행하였으며 연구대상자들의 구급활동일지와 구급지령서를 분석하였다. 119구급상황관리센터 상황요원(Dispatcher)이 가장 많이 예측한 지령은 실신으로 74건(33.3%)이었다. 다양하게 예측된 지령 중 실신, 흉통, 전신쇠약, 기타와 같은 지령은 심정지로 예측 불가능한 지령으로 112건(50.5%)을 차지하였으며, 심정지로 예측 가능한 의식장애, 호흡곤란, 무호흡, 심정지, 경련과 같은 지령은 110건(49.5%)을 차지하였다. 이 때 심정지로 예측 가능한 지령에서 목격자의 심폐소생술이 유의하게 높았다. 이와 같이 상황요원은 환자의 의식평가와 호흡평가를 구체적으로 질문하여 심정지를 정확하게 분류해야하며, 이렇게 분류된 심정지 환자는 목격자가 심폐소생술을 할 수 있도록 지도해야 한다. 또한 상황요원에 의해 심정지로 예측 가능한 지령(49.5%)을 받은 구급대원뿐만 아니라 심정지로 예측 불가능한 지령(50.5%)에서도 심정지가 아니라고 정확하게 판단되지 않았다면 현장 구급대원은 심정지 상황에 대비하여 현장출동 준비를 해야 한다.

• 한국치위생학회지
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• 제18권4호
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• pp.477-488
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• 2018

Objectives: The purpose of this study was to investigate career competency, tasks, and job satisfaction of public servants, public institutions, and researchers. Methods: The survey was conducted about career competency, job satisfaction, and satisfaction on work life. Next, they interviewed on the characteristics of each job by two or three dimensions. The following conclusions were obtained from July to August 2017. Results: Career competencies were GPA with 3.87, 818 points of TOEIC score, and ITQ certification. Public servants required the information on literacy skills for employment and job performance, while civil servants need more than one year of clinical experience in the dental hospital. The non-commissioned officer needed a written test and fitness training. The health insurance review and assessment center required more than one year of experience from general hospital or medical institutions. Researchers required a research career, language skill, and professors required research and teaching experiences with clinical experience more than three years. The main job tasks were as follows; for public servants, they were official document processing and community projects. For the civilian workers and military/noncommissioned officers, they were medical assistant and administrative works. The employees of the health insurance review and assessment service are examining the medical expenses and the medical examination, the researchers are experimenting, researching and writing articles, and the teaching staff are lecturing and conducting individual research. Conclusions: The results of job satisfaction survey showed that occupational satisfaction was the highest in civil servants, researchers, and teaching professions. Job security was the highest in health workers and health inspectors' evaluation centers, and time vacancy was the highest in civilian workers and military/noncommissioned officers. If you want to work in such an institution, you should prepare elements that match your basic literacy and job specific characteristics. And we should try to increase the satisfaction of work even after work.

• Korean Journal of Acupuncture
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• 제34권3호
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• pp.109-115
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• 2017

Objectives : Many researches have studied warm needling technique to standardize its treatment by temperature measurement and material differences in the effectiveness. The purpose of this study is to compare the temperature changes of the acupuncture needle shaft during the combustion process of the moxa stick to determine the heat transfer pattern of the warn needling. Methods : A moxa stick($7{\times}8mm$) was connected to one side of the needle shaft using a stainless steel needle(ø 0.3 mm, ø 0.5 mm, ø 0.8 mm, shaft length 40 mm) with the needle handle removed. During the warm needling, temperature changes of the needle shaft were observed with an infrared camera(Flir E30) and an infrared thermometer(TESTO 845). Results : In the normal condition, heat transmit of needle shaft increased at spots 10 mm and 25 mm below the moxa stick. The amount of heat transmit increased with the diameter of needle shaft. However, when the heat shield was installed to exclude radiant heat from the moxa stick, heat transfer was less at 10 mm below the moxa stick and no temperature change was observed at 25 mm below the moxa stick. Heat transfer by warm needling does not reach the end of needle shaft even in ø 0.8 mm needle. Conclusions : It is suggested that the radiant heat of moxa stick results in the heat transmit of acupuncture needle shaft. Thus, radiant heat transmit must be considered as one of the heat transfer characteristics of the warm needling.

• 한국보건간호학회지
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• 제32권2호
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• pp.288-303
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• 2018

Purpose: This study was a descriptive research to provide basic data for the development of a customized emergency education program to strengthen the capacity of first aid for nursing caregivers. Method: The number of caregivers in region G was 193, and data collection was conducted from April 1 to April 30, 2018. Data were analyzed using a t-test, one-way ANOVA, Pearson's correlation coefficients, and Scheffe test. Results: In the study group 47.2% experienced an emergency situation. The first aid knowledge was 10.21; the correct answer rate was 56.0%; the basic CPR knowledge was 3.99; the correct answer rate was 49.7%. After asking for help, first aid was the most difficult. In general first aid knowledge was 6.21 points and the correct answer rate was 62.2%. They understood well about strokes, injuries and trauma, but had difficulty in consciousness, depression, and burns. For first aid knowledge, there were significant differences according to age, working hours, place of work, working experience, emergency situation, and education. Of the study group, 59.6% experienced emergency training, 96.9% wanted it, and 72.0% had low emergency response skills. Moreover, 93.3% answered for having emergency education. Conclusion: The results suggest that a personalized emergency education program for nursing caregivers should be developed and applied.

• Korean Journal of Acupuncture
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• 제33권4호
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• pp.194-203
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• 2016

Objectives : The aim of this study was to observe the dose-dependent effects of bee venom (BV) pharmacopuncture on the serious ankle sprain in rats. Methods : The grade III ankle sprain was produced by surgically damaging the lateral ligaments complex of Sprague-Dawley rats. BV pharmacopuncture with the different doses($5{\mu}g/kg$, $10{\mu}g/kg$, $50{\mu}g/kg$) were treated on the different acupoints(GB34, GB39 and GB42) of the affected hind limb, respectively. By measuring foot weight bearing force ratio(FWBFR), the pain levels by ankle sprain and the pain recovery for 7 days were observed under BV pharmacopuncture on each acupoint. Results : In the normal and ankle sprained rat, the BV single administration decreased FWBFR in a dose - dependent pattern. The higher the BV dose, the higher the pain resulted in the normal and the sprained ankles. Especially, the dose - dependent effects of BV resulted in the most pronounced decrease in FWBFR in GB34. The recovery of FWBFR was shown at the low dose($5{\mu}g/kg$) BV and the effect was most remarkable in the BV of GB34, but the others showed no recovery effect compared with the control group. Conclusions : BV pharmacopuncture does not exhibit analgesic effects in acute phase of ankle sprain. However, the recovery of ankle sprain was more effective than the natural recovery in the case of low dose of BV repeated over time. Considering this, it is presumed that it would be important to select appropriate clinic guidelines for acute phase of ankle sprain.

• Korean Journal of Acupuncture
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• 제35권1호
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• pp.27-35
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• 2018

Objectives : The purpose of this study was to investigate the analgesic effect of electroacupuncture(EA) and radio-frequency warm needling(RFWN) stimulation in acupoint combination on ankle sprained pain in rats. Methods : The lateral ligaments of the Sprague-Dawley rats ankle were injured surgically resulting in sprain, of which was divided into EA, RFWN treatment groups and control group without treatment. The level of pain was measured through foot weight bearing force ratio followed by calculating pain relief. To stimulate proximal or distal area in ankle sprain, combination of proximal acupoints(GB34-GB39) and distal acupoints(GB39-GB42) from sprain area were applied, respectively, to either EA or RFWN stimulation. In addition, naltrexone or phentolamine was injected intraperitoneally before the stimulation to observe the pathway of analgesic effects. Results : In the proximal combination of GB34-GB39, EA and RFWN significantly increased pain relief compared to the control group (p<0.05). However, in distal combination with GB39-GB42, both EA and RFWN stimulation did not relieve pain due to ankle sprains. In the combination of GB34-GB39, the analgesia of EA was inhibited by blockade of the ${\alpha}$-adrenoceptor receptor. The analgesia of RFWN was inhibited by blockade of the ${\alpha}$-adrenoceptor receptor as well as ${\mu}$-opioid receptor. Conclusions : We observed that the proximal combination was effective in relieving pain when the treatment by acupoint combination was applied to the ankle sprain pain. Also, it was confirmed that this analgesia was also related to the pathways of ${\mu}$-opioid receptors and/or ${\alpha}$-adrenoceptors.

• 한국간호교육학회지
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• 제24권4호
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• pp.347-357
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• 2018

Purpose: The purpose of this study was to identify patient safety awareness and emergency response ability and affecting factors perceived by nursing homes and home visiting caregivers. Methods: This study was a descriptive study that conveniently extracts nursing caregivers who care for elderly patients in S and G provinces, Korea. Data collection was done by structural questionnaires from April to May 2018. A total of 204 responses consisting of 103 nursing homes and 101 home visiting caregivers were used for data analysis in SPSS Win 22.0. Results: Patient safety awareness and emergency response ability of nursing homes caregivers with each $4.24{\pm}0.50$, $74.26{\pm}09.57$ was each higher than that of the home visiting caregivers with $3.68{\pm}0.49$, $68.02{\pm}12.12$ (p<.001). The affecting factors of the patient safety awareness were working place, safety education, and daily average working hours with 12 or more (F = 27.30, p<.001) and that of emergency response ability were number of patients per personnel with 9 or more and emergency situation experience (F=14.00, p<.001). Conclusion: These results suggest that it is necessary to develop a safety education program that can share indirectly experience emergency situations that occur on the job site.

• 대한임상독성학회지
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• 제17권2호
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• pp.66-78
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• 2019

Purpose: Thallium (TI+) autometallography is often used for the imaging of neuronal metabolic activity in the rodent brain under various pathophysiologic conditions. The purpose of this study was to apply a thallium autometallographic technique to observe changes in neuronal activity in the forebrain of rats following acute carbon monoxide (CO) intoxication. Methods: In order to induce acute CO intoxication, adult Sprague-Dawley rats were exposed to 1100 ppm of CO for 40 minutes, followed by 3000 ppm of CO for 20 minutes. Animals were sacrificed at 30 minutes and 5 days after induction of acute CO intoxication for thallium autometallography. Immunohistochemical staining and toluidine blue staining were performed to observe cellular damage in the forebrain following intoxication. Results: Acute CO intoxication resulted in significant reduction of TI+ uptake in major forebrain structures, including the cortex, hippocampus, thalamus, and striatum. In the cortex and hippocampal CA1 area, marked reduction of TI+ uptake was observed in the cell bodies and dendrites of pyramidal neurons at 30 minutes following acute CO intoxication. There was also strong uptake of TI+ in astrocytes in the hippocampal CA3 area following acute CO intoxication. However, there were no significant histological findings of cell death and no reduction of NeuN (+) neuronal populations in the cortex and hippocampus at 5 days after acute CO intoxication. Conclusion: The results of this study suggest that thallium autometallography can be a new and useful technique for imaging functional changes in neural activity of the forebrain structure following mild to moderate CO intoxication.

• 한국산업융합학회 논문집
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• 제24권6_2호
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• pp.809-818
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• 2021

The purpose of this study was to identify the influence of oral health knowledge and awareness of caregivers in charge on the oral health-related quality of life of the elderly in nursing homes. Data were collected from 115 elderly without dementia and their 115 caregivers in nursing facilities in S and C cities. The data were analyzed using SPSS/WIN 22.0 program. The average score for oral health knowledge and awareness of the caregivers were 11.62, 39.22 points each and the oral health-related quality of life of the elderly was 40.62 points. Oral health knowledge, awareness of caregivers and oral health-related quality of life of the elderly showed a difference according to oral health education experience of the caregivers (𝜌<.001), the nursing facility evaluation grade (𝜌=.016), and the oral health education experience (𝜌=.008), working hours of 40 hours or less per week of caregivers (𝜌=.008) each in order. The influencing factors on the oral health-related quality of life of the elderly were the oral health education experience, the working hours per week of the caregivers and the facility evaluation grade. This findings imply that developing customized program and the work environment improvement for caregivers should be considered to improve the oral health-related quality of life of the elderly in nursing homes.