• Parasites, Hosts and Diseases
• /
• 제60권4호
• /
• pp.281-288
• /
• 2022

Malaria continues to be one of the most crucial infectious burdens in endemic areas worldwide, as well as for travelers visiting malaria transmission regions. It has been reported that 8-aminoquinolines are effective against the Plasmodium species, particularly primaquine, for anti-hypnozoite therapy in P. vivax malaria. However, primaquine causes acute hemolytic anemia in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Therefore, G6PD deficiency testing should precede hypnozoite elimination with 8-aminoquinoline. Several point-of-care devices have been developed to detect G6PD deficiency. The aim of the present study was to evaluate the performance of a novel, quantitative G6PD diagnostics based on a metagenomic blue fluorescent protein (mBFP). We comparatively evaluated the sensitivity and specificity of the G6PD diagnostic modality with standard methods using 120 human whole blood samples. The G6PD deficiency was spectrophotometrically confirmed. The performance of the G6PD quantitative test kit was compared with that of a licensed control medical device, the G6PD strip. The G6PD quantitative test kit had a sensitivity of 95% (95% confidence interval (CI): 89.3-100%) and a specificity of 100% (95% CI: 94.3-100%). This study shows that the novel diagnostic G6PD quantitative test kit could be a cost-effective and time-efficient, and universally mandated screening tool for G6PD deficiency.

• 대한핵의학회지
• /
• 제34권4호
• /
• pp.353-359
• /
• 2000

목적: 최근 국내에서 방사면역측정법용 ${\alpha}$-FP 검사시약(Riakey AFP IRMA $CT^R$, Sin-Jin Medics, Seoul, Korea)이 개발되어 그 성능을 평가해 보고자 본 연구를 시행하였다. 대상 및 방법: 검사방법은 시약 내 설명서의 권고사항을 준수하여 실시하였으며, 정밀도, 회수율, 평행성, 민감도에 대한 검사를 시행하였고, 다른 상업용 방사면역측정법용 a-FP 검사시약에 의한 측정값과의 상관관계를 조사하였다. 결과: 측정 내 정밀도는 세 준위의 혈청을 각각 10개의 튜브에 분주한 후 한 배치에서 검사하였는데, 평균 18.9 ng/ml에서 변이계수 5.3%, 평균 133 ng/ml에서 3.4%, 평균 332 ng/ml에서 변이계수 1.6%였다. 측정간 정밀도는 세 준위의 혈청을 하루 간격으로 5회 측정하여, 평균 20.9 ng/ml에서 변이계수 9.7%, 평균 137 ng/ml에서 3.2%, 평균 330 ng/ml에서 변이계수 4.1%였다. 회수율은 저, 중, 고 세 가지 농도의 혈청에 각각 시약 내 표준용액 10, 70, 250 ng/ml을 1:1로 섞은 후 검사하여, 17.6 ng/ml에서 각각 102%, 105%, 101%, 105 ng/ml에서 각각 103%, 95%, 105%였고, 245 ng/ml에서 각각 97.3%, 92.1%, 98.6%이었다. 평행성 검사는 세 개의 고농도 검체를 각각 2, 4, 8, 10, 20, 100 배 희석하여 한번에 검사하였으며, 평행성 검사에서 계산한 수식은 각각 y=0.98x-10.3, y=0.99x-8.32, y=0.98x-11.3이었다. 민감도는 측정가능한 최소농도가 0.63 ng/ml이었다. 175명의 환자검체의 측정결과를 다른 시약들로 측정한 것과 비교하였을 때 강한 상관관계를 보였다(r=0.98). 결론: 이상의 결과로 ${\alpha}$-FP 검사시약인 Riakey AFP IRMA $CT^R$은 임상에 적용할 수 있는 유용한 시약으로 판단된다.

• Tuberculosis and Respiratory Diseases
• /
• 제55권4호
• /
• pp.388-394
• /
• 2003

배 경 : 지금까지 폐암의 종양표지자로 알려진 것들 중에 최근에는 CK 19뿐만 아니라 폐암에서 많이 발견되는 CK 8, 18에 대해 특이적인 세가지 단클론항체를 이용한 면역방사선계수법(MonoTotal$^{TM}$)이 개발되어 폐암의 진단에 이용할 수 있는 것으로 보고되었다. 따라서 저자들은 Cyfra 21-1에 비교하여 CK 8, 18, 19의 폐암표지자로서의 진단적 가치에 대해 연구하였다. 대상 및 방법 : 2002년 5월 l일부터 2002년 9월 30일까지 서울대학교병원 내과에 입원하여 기관지내시경 또는 객담도말세포검사, 경피적 조직생검으로 확진된 원발성 폐암환자 129명을 폐암군으로 하고 폐암이 배제된 비악성 호흡기 질환자 160명을 환자 대조군으로 연구에 동의한 건강한 성인 57명을 정상 대조군으로 하여 본 연구를 시행하였다. Cyfra 21-1의 측정은 Fujibebio Diagnostics사(USA)의 immnoradiometric assay kit인 Cyfra$^{TM}$ 21-1을 사용하였고 CK 8, 18, 19의 측정은 IDL biotech사의 2-hour immunoradiometric sandwich test kit인 Monototal$^{TM}$을 사용하였다. 결 과 : 폐암의 조직학적 분류에 따른 Monototal의 혈중평균치(mean${\pm}$SD)는 비소세포폐암이 $412.47{\pm}455.45$ U/L, 소세포폐암이 $237.08{\pm}145.15$ U/L로 환자 대조군 $126.54{\pm}95.72$ U/L, 정상 대조군 $63.68{\pm}31.66$ U/L에 비해 유의하게 증가되어 있었다(p<0.01). Monotoal의 정상범위를 정상 대조군의 가장 높은 수치인 188U/L 이내로 하였을때 비소세포폐암의 민감도는 66.4%, 특이도는 81.9%였다. 병기를 I,II군(32명)과 III,IV군(84명)의 두 군으로 나누어 Monototal의 평균을 비교해 보았을 때 각각 $227.6{\pm}185.2$ U/L, $482.9{\pm}506.4$ U/L로 두 군간에 통계적으로 유의한 차이가 있었다(p<0.001). 환자대조군 세부질환별로 Monototal의 평균치를 보면 미만성폐질환 환자군(11명)에서 $298.7{\pm}210.0$ U/L로 환자 대조군 평균치인 $126.54{\pm}95.72$ U/L에 비해 통계적으로 유의하게 높게 측정되었다(p<0.001)(Table 4). 결 론 : cytokeratin 8, 18, 19의 측정은 폐암의 대표적인 종양 표지자중의 하나인 Cyfra 21-1과 비슷한 민감도와 특이도를 보이고 있어 효과적인 종양표지자로 앞으로 사용될 수 있다.

• 핵의학기술
• /
• 제19권2호
• /
• pp.102-107
• /
• 2015

Purpose Serum Thyroglobulin measurement is a major tool for the follow-up of differentiated thyroid cancer (DTC) patients. Thyroglobulin is Normal thyroid tissue, or thyroid cancer tissue produced only. Thyroid hormone to a halt without Tg differentiation of thyroid cancer recurrence just by measuring how to decide whether there was increasing expectations if I can do it instead. Therefore, in excellent sensitivity Tg new inspection of the functional sensitivity by measuring the looked to evaluate the usefulness of reagents. Thyroidectomy is measuring the numbers Tg (total thyroidectomy) remaining thyroid ablation and radioactive iodine (radioactive iodine remnant ablation, RRA) DTC in patients being diagnosed with or help predict the remaining early detection of thyroid cancer recurrence. Materials and Methods Agent that I'm currently using Tg of the measurements of low clinical specimen for a second drainage of the three (0.08 0.17, 0.98, ng/mL) within the scope of the dilute magnification (2, 4, 8, 16, 32 times) by dilute Intraassy (n=10) and Interassay (n=10) out in no time. Concentration value according to the coefficient of variation and the mean and standard deviation of each measurement (Coefficient of variation, CV) the absolute value of the measured values that corresponds to 20 percent target a coefficient of variation of CV Find the value of the concentration of the functional sensitivity measurement did. Also, analytical sensitivity with recovery rates, Dilution test inspections, and interrelationship, compared. Results Sensitivity is an excellent analytical sensitivity within the prosecutor kit Tg 0.006 ng/mL, and core analytical sensitivity, conducted by the 0.006 ng/mL to same conclusion. Be rather high to 142 percent recovery rate was 60 to measurement and functional sensitivity, 0.01766 ng/mL(Intraassay n=10) was measured at. CBC is relatively good correlation as ($R^2=0.949$) the correlation. Conclusion Recently ultrasensitive thyroglobulin this clinically important indicators of the previous kit and demands are lower than sensitivity to the measurement results. Therefore, ultrasensitive thyroglobulin test is correlated that there would be useful in value in nuclear medicine the thyroid gland.

• 대한임상검사과학회지
• /
• 제41권4호
• /
• pp.145-152
• /
• 2009

Novel influenza A virus, subtype H1N1 of swine-lineage, has been transmitted rapidly to many regions of the world. Rapid detection of the virus is essential to instigate appropriate patient care and public health management and for disease surveillance. The aim of this study is to determine the prevalence of novel influenza A (H1N1) virus in Korea using reverse-transcription real time polymerase chain reaction (rRT-PCR). Novel H1N1 virus was detected in a total of 8,948 nasopharyngeal samples from patients with influenza-like illness throughout Korea from August to September 2009. RNA was extracted from $300{\mu}l$of sample using an RNA extraction kit (Zymo Research, CA, USA). In the present study, Genekam kit (Genekam, Duisburg, Germany) was used to detect novel H1N1 virus. Novel H1N1 virus was found in 1,130 samples from a total of 8,948 samples (12.6%). The highest frequency was found in 10- to 19-year-olds (M: 29.3% vs. F: 16.4%), followed by 20- to 29-year-olds (M: 17.9% vs. F: 15.4%), 40- to 49-year-olds (M: 6.5% vs. F: 8.1%), 50- to 59-year-olds (M: 6.0% vs. F: 5.5%), and 30- to 39-year-olds (M: 4.6% vs. F: 3.8%). The mean positive rate was higher in men than in women (M: 14.7% vs. F: 7.4%). Novel H1N1 virus showed the lowest prevalence in patients over 60 years old. The positive rate increased daily and showed a significant high peak in mid-September 2009. In 19 provinces of Korea, Cheonan (41.1%), Busan (37.3%), Gangneung (33.3%), Jinju (32.1%), Ulsan (24.6%), Deajeon (23.7%) areas showed high frequencies and other provinces were found less than 10% of novel H1N1 virus. Since reverse-transcription real time PCR assay is rapid, accurate, and convenient, it may assist public health laboratories in detecting novel H1N1 virus. Moreover, these data could be useful for the management of patients with influenza-like illness.

• 한국임상수의학회지
• /
• 제38권4호
• /
• pp.184-188
• /
• 2021

A 10-year-old spayed female Maltese with a history of vomiting and lethargy was referred to the hospital. Physical examination revealed dehydration and severe pain following abdominal palpation. A large mass was observed in the cranial abdomen through radiography and ultrasonography. Laparotomy was performed to find the origin of the mass. The mass was about 8 cm originating from the cecum and subsequently removed. Histopathologic evaluation revealed that the cecal mass was suspected to be a mesenchymal-derived tumor. Through immunohistochemistry, the mass was diagnosed as a gastrointestinal stromal tumor (GIST) based on the c-kit expression. Given its recurrence, postoperative preventive therapy was initiated with masitinib mesylate, which is a tyrosine kinase inhibitor. The animal did not show any side effects during the medication period. After 6 months of therapy, it was well controlled without any recurrence. In this case, we introduced a novel postoperative management of GIST using masitinib mesylate.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권15호
• /
• pp.6099-6101
• /
• 2014

Purpose: To retrospectively analyze variability and clinical significance of serum ferritin levels in Chinese patients with hematologic malignancies. Materials and Methods: Serum ferritin were measured by radioimmunoassay, using a kit produced by the Beijing Institute of Atomic Energy. Patients with hematologic malignancies, and treated in the Department of Hematology in Nanjing First Hospital and fulfilled study criteria were recruited. Results: Of 473 patients with hematologic malignancies, 262 patients were diagnosed with acute leukemia, 131 with lymphoma and 80 with multiple myeloma. Serum ferritin levels of newly diagnosed and recurrent patients were significantly higher than those entering complete remission stage or in the control group (p<0.001). Conclusions: Serum ferritin lever in patients with hematologic malignancies at early stage and recurrent stage are significantly increased, so that detection and surveillance of changes of serum ferritin could be helpful in assessing conditions and prognosis of this patient cohort.

• The Korean Journal of Pain
• /
• 제9권1호
• /
• pp.200-205
• /
• 1996

Radiofrequency thermocoagulation(RF) techniques are safe and effective methods as compared to neurodestructive procedure. Other advantages are: ability to perform RF lesions under local or sedative anesthesia, rapid recovery period, low incidence of morbidity and mortality, ability to repeat RF lesions, and leaves no significant scarring. We performed C2 ganglionotomy by RF lesion generator on a patient, suffering post-traumatic occipital neuralgia, as the patient did not respond to conservative therapies such as: trigger point injection, TENS, cryotherapy and stretch, occipital nerve block, C2 ganglion block. Prognostic nerve block was performed usng local anesthetics. Excellent effect was conformed before C2 ganglionotomy. This procedure was performed under fluoroscopy. Type RCK-2A Rosomoff Cordotomy kit was used to stabilize the head and neck. Postoperatively, the patient was free of occipital pain and head motions no longer triggered pain. To date, the patient remains symptom free except for some cervical discomfort.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권11호
• /
• pp.4663-4670
• /
• 2014

Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. We here investigated its effects on proliferation and apoptosis of the CNE2 carcinoma cell line, and attempted to establish genome-wide DNA methylation alteration due to differentially histone acetylation status. After cells were treated by TSA, the inhibitory rate of cell proliferation was examined with a CCK8 kit, and cell apoptosis was determined by flow cytometry. Compared to control, TSA inhibited CNE2 cell growth and induced apoptosis. Furthermore, TSA was found to induce genome-wide methylation alteration as assessed by genome-wide methylation array. Overall DNA methylation level of cells treated with TSA was higher than in controls. Function and pathway analysis revealed that many genes with methylation alteration were involved in key biological roles, such as apoptosis and cell proliferation. Three genes (DAP3, HSPB1 and CLDN) were independently confirmed by quantitative real-time PCR. Finally, we conclude that TSA inhibits CNE2 cell growth and induces apoptosis in vitro involving genome-wide DNA methylation alteration, so that it has promising application prospects in treatment of NPC in vivo. Although many unreported hypermethylated/hypomethylated genes should be further analyzed and validated, the pointers to new biomarkers and therapeutic strategies in the treatment of NPC should be stressed.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권16호
• /
• pp.6581-6586
• /
• 2014

Background: The istone deacetylase (HDAC) inhibitor trichostatin A (TSA) is known to mediate the regulation of gene expression and antiproliferation activity in cancer cells. Kr$\ddot{u}$ppel-like factor 4 (klf4) is a zinc finger-containing transcription factor of the SP/KLF family, that is expressed in a variety of tissues and regulates cell proliferation, differentiation, tumorigenesis, and apoptosis. It may either either function as a tumor suppressor or an oncogene depending on genetic context of tumors. Aims: In this study, we tested the possibility that TSA may increase klf4 expression and cancer cell growth inhibition and apoptosis in SKOV-3 and A549 cells. Materials and Methods: The cytotoxicity of TSA was determined using the MTT assay test, while klf4 gene expression was assessed by real time PCR andto ability of TSA to induce apoptosis using a Vybrant Apoptosis Assay kit. Results: Our results showed that TSA exerted dose and time dependent cytotoxicity effect on SKOV-3 and A549 cells. Moreover TSA up-regulated klf4 expression. Flow cytometric analysis demonstrated that apoptosis was increased after TSA treatment. Conclusions: Taken together, this study showed that TSA increased klf4 expression in SKOV3 and A549 cell lines, consequently, klf4 may played a tumor-suppressor role by increasing both cell growth inhibition and apoptosis. This study sheds light on the details of molecular mechanisms of HDACI-induced cell cycle arrest and apoptosis.