• Title/Summary/Keyword: Media System

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Effect of Callus Type and Antioxidants on Plant Regeneration and Transformation of Tall Fescue (캘러스의 형태와 항산화물질 첨가가 톨 페스큐의 식물체 재분화와 형질전환효율에 미치는 영향)

  • Lee Ki-Won;Lee Sang-Hoon;Kim Do-Hyun;Lee Dong-Gi;Won Sung-Hye;Lee Hyo-Shin;Lee Byung-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.26 no.2
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    • pp.77-82
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    • 2006
  • An efficient transformation system for the production of transgenic plants has been developed for tall fescue (Festuca arundinacea Schreb.) via Agrobacterium-mediated transformation of seed-derived callus. From the point of morphogenetic capacity, three types of callus were selected. High frequency of plant regeneration was obtained by selection of type II callus, and the plant regeneration frequency was 52.6% when embryogenic callus were cultured on the regeneration medium. Supplementation of the media with 10 mg/L $AgNO_3$ and 40 mg/L cysteine enhanced frequencies of plant regeneration up to 65.3%. The highest transformation efficiency was also obtained when type II callus were inoculated with Agrobacterium. Southern blot analysis of PCR products of transgenic plants demonstrated that transgenes were successfully integrated into the genome of tall fescue. Efficient regeneration system and transformation established in this study will be useful for molecular breeding of tall fescue through genetic transformation.

A study of next generation OpenCable systems for Ultra-High Definition television broadcasting (초 고화질 텔레비전 방송을 위한 차세대 오픈 케이블 방식에 대한 연구)

  • Cho, Chang-Yeon;Heo, Jun;Kim, Joon-Tae
    • Journal of Broadcast Engineering
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    • v.14 no.2
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    • pp.228-237
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    • 2009
  • This paper examines the potential of Ultra-High Definition TV (UD-TV) broadcasting transmission systems beyond HD-TV over cable channel. Firstly, we analyze the trend of TOV(Threshold of Visibility) by extending the OpenCable (J.83 Annex B) system 256QAM which is the standard of Korean and American cable television transmission to 1024QAM, and realize that the OpenCable 1024QAM has nearly 30% higher data rate than 256QAM at the expense of impractically higher TOV (Threshold of Visibility). To achieve practical TOV, we control code rates of inner convolutional coder and replace turbo coder in forward error correction (FEC) part, thereby analyzing the best performance of the OpenCable systems having conventional FEC. In that result, it is necessary to modify conventional FEC of the OpenCable system to achieve under 31.5dB TOV. Moreover we study the potential of UD-TV transmission via two or more TV channels, so called channel bonding, through the Shannon capacity in 6MHz channel and the relationship with next generation A/V codec technologies.

An Analysis of Optimal Sequences for the Detection of Wake-up Signal in Disaster-preventing Broadcast (재난방송용 대기모드 해제신호 검출을 위한 최적 부호 성능 분석)

  • Park, Hae Yong;Jo, Bonggyun;Kim, Heung Mook;Han, Dong Seog
    • Journal of Broadcast Engineering
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    • v.19 no.4
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    • pp.491-501
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    • 2014
  • Recently, the need for disaster-preventing broadcast has increased gradually to cope with natural disaster like earthquake and tsunami causing enormous losses of both life and property. In disaster-preventing broadcast system, the wake-up signal is used to alert user terminal and switch the current state of channel to the emergency channel, which is for the fast and efficient delivery of emergency information. In this paper, we propose the detection method of wake-up signal for disaster-preventing broadcast systems. The wake-up signals for disaster-preventing broadcast should have a good auto-correlation property in low power and narrow-band conditions that does not affect the existing digital television (DTV) system. The suitability of the m-sequence and complementary code (CC) is analyzed for wake-up signals according to signal to noise ratio. A wake-up signal is proposed by combining the direct sequence spread spectrum (DSSS) technique and pseudo noise (PN) sequences such as Barker and Walsh-Hadamard codes. By using the proposed method, a higher detecting performance can be achieved by the spreading gain compared to the single long m-sequence and the Golay code.

Optimum Configuration of Single Frequency Network DMB to enhance the QoS and Service coverage (QoS 개선과 서비스 커버리지 확장을 위한 단일 주파수망 지상파 DMB 최적화 배치)

  • Cho, Young-Hun;Won, Chung-Ho;Seo, Jong-Soo
    • Journal of Broadcast Engineering
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    • v.19 no.4
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    • pp.439-452
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    • 2014
  • This paper suggests the method to extend service area by using the transmit offset delay in T-DMB single frequency network (SFN). In general, synchronization of the transmit time of all site can be done by using the reference clock of GPS, which dose not reflect the details geographical characteristics and transmit specifications of each site. Applying the site-specific transmit offset delay, we could extend the service area of SFN T-DMB. Applying the transmit offset delay, it is found that the signal quality in the region of weak receive field strength was improved and upto 4~8 km service area expansion was achieved by satisfying the minimum field strength ($45dB{\mu}V/m$) recommended by the Korea Communications Commission (KCC). Site-specific offset delay was calculated considering the geographic service area characteristics, distribution of electric field strength between neighboring sites and site-specific service target area. Experiments were carried out in order to analyze impact of calculated offset delay on the T-DMB SFN and also to confirm that the offset delay extends T-DMB service coverage. The experiment was done in metropolitan T-DMB service areas.

Innovative Geostationary Communication and Remote Sensing Mutli-purpose Satellite Program in Korea-COMS Program

  • Baek, Myung-Jin;Park, Jae-Woo
    • Journal of Satellite, Information and Communications
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    • v.2 no.2
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    • pp.29-35
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    • 2007
  • COMS satellite is a multipurpose satellite in the geostationary orbit, which accommodates multiple payloads of the Ka band Satellite Communication Payload, Meteorological Imager, and Geostationary Ocean Color Imager into a single spacecraft platform. In this paper, Korea's first innovative geostationary Communication, Ocean and Meteorological Satellite (COMS) program is introduced which is fully funded by Korean Government. The satellite platform is based on the Astrium EUROSTAR 3000 communication satellite, but creatively combined with MARS Express satellite platform to accommodate three different payloads efficiently for COMS. The goals of the Ka band satellite communication mission are to in-orbit verify the performances of advanced communication technologies and to experiment wide-band multi-media communication service. The Meteorological Imager mission is to continuously extract meteorological products with high resolution and multi-spectral imager, to detect special weather such as storm, flood, yellow sand, and to extract data on long-term change of sea surface temperature and cloud. The Geostationary Ocean Color Imager mission aims at monitoring of marine environments around Korean peninsula, production of fishery information (Chlorophyll, etc.), and monitoring of long-term/short-term change of marine ecosystem. The system design difficulties are in the different kinds of payload mission requirements of communication and remote sensing purposes and how to combine them into one to meet the overall satellite requirements. In this paper, Ka band communication payload system is more highlighted.

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Compensation Analysis of Cell Delay Variation for ATM Transmission in the TDMA Method (TDMA 방식에서 ATM 전송을 위한 셀 지연 변이의 보상 해석)

  • Kim, Jeong-Ho;Choe, Gyeong-Su
    • The Transactions of the Korea Information Processing Society
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    • v.3 no.2
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    • pp.295-304
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    • 1996
  • Toprovide economical BISDN service, with which integration process of many types of media is possible, it is necessary to construct a system with ground network and satellite network combined. The method for this type of transmission using satellite is TDMA that can provide services to many users in various area. However, the most difficult task to connect TDMA which uses synchronous method to ATM which used asynchronous transfer mode is the deterioration n of ATM transmission quality such as cell delay variation. Therefore, it is necessary to develop delay variation compensation method which can confront to the ATM. Efficient ways to use satellite links under the conditions such that maximum efficiency of the delay variation is limited under the required value, and the burst characteristic of transmission cell does not increase are being researched for translation between in ATM and TDMA. This paper points out the problems when time stamp method, reviewd in ground network, is applied to the satellite links to compensate the delay variation .To solve the problem, discrete cell count method is introduced along with the calculation of transmission capacity and error rate.Also, from the observation of stab-ility of the system and verification of reliability even when singal error occurred in the cell transmission timing information, the proposed compensation method appeared to be excellent.

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Design of MPEG-2 Transport Stream specification for stereoscopic video broadcasting service (스테레오스코픽 비디오 방송 서비스를 위한 MPEG-2 전송스트림 구성 방안)

  • Park, Heung-Sik;Kim, Kyu-Heon;Lee, Gun-Hee;Yun, Kug-Jin;Suh, Doug-Young;Park, Gwang-Hoon
    • Journal of Broadcast Engineering
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    • v.14 no.6
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    • pp.769-782
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    • 2009
  • Stereoscopic video service is one of the most widely adopted technologies for 3-Dimensional technology. However, the stereoscopic video service has not been penetrated into the market since there does not exist a proper transmission method even though the conventional MPEG-2 Transport Stream (TS) has support for broadcast service of mono-scopic video contents. This is partly because the MPEG-2 TS does not support displaying a stereoscopic video content which consists of several composition types widely used in the market. This paper proposes the new MPEG-2 TS specification suitable for transmitting and displaying stereoscopic video contents. In order to show feasibility of the proposal, this paper describes an end-to-end system which is implemented with consideration of the method how to multiplex and de-multiplex stereoscopic video contents as well as keeping backward compatibility with the traditional MPEG-2 TS.

A Study on Real-time Implementing of Time-Scale Modification (음성 신호 시간축 변환의 실시간 구현에 관한 연구)

  • Han, Dong-Chul;Lee, Ki-Seung;Cha, Il-Hawan;Youn, Dae-Hee
    • The Journal of the Acoustical Society of Korea
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    • v.14 no.2
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    • pp.50-61
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    • 1995
  • A time scale modification method yielding rate-modified speech while conserving the characteristic of speech was implemented in real-time using a goneral purpose digital signal processor. Time scale modification changed pronunciation speed only, producing a time difference between the input signal and the modified signal, making it impossible to implement it in real-time. In this thesis, a system was implemented to remove the time difference between the input and modified signals. Speech signals slowed down or speeded up by a physical time scale modification method, such as adjusting the motor speed of the cassett tape recorder, was used as the input signal. Physical modification that controled only the inter speed of the cassette tape player distorted the pitch period of the original speech. In this study, a real-time system was implemented so that the pitch-distorted speech was reconstructed back to the original by fractional sampling pitch shifting using an FIR filter, and this signal was time scale modified to match the cassette tape recorder motor speed using SOLA time-scale medification. In experiments using speech signals medifiedby the proposed method, results obtained using a 16-bit resolution ADSP2101 processor and using computer simulations employing floating point operations showed about the same average frame signal-to-noise ratio of about 20 dB.

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The Effects of Elevated CO2 and Ammonium Levels in Seawater on the Physiology of Gracilariopsis chorda (Holmes) Ohmi

  • Kang, Jin Woo;Chung, Ik Kyo
    • Journal of Environmental Science International
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    • v.25 no.4
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    • pp.473-481
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    • 2016
  • We examined the effects of ocean acidification (OA) and eutrophication on the physiology of a red alga, Gracilariopsis chorda, using specimens collected at Wando Island, Korea, in July of 2015. The samples were transported to a laboratory and placed on growth media for treatments involving low or high levels of ammonium ($4{\mu}M$ or $60{\mu}M\;NH_4{^+}$) and low or high pH(7.5 or 8.2). The control treatment used filtered seawater (pH 8.2 and $4{\mu}M\;NH_4{^+}$). All experiments were conducted at $20^{\circ}C$ and under a lighting intensity of $80{\mu}mol\;photons\;m^{-2}\;s^{-1}$, with or without an injection of $CO_2$ (pH 7.5). In addition, we calculated rates of respiration under darkness, at a pH of 7.5 and $60{\mu}M\;NH_4{^+}$. Fluctuations in pH as well as the evolution of photosynthetic oxygen and $NH_4{^+}$ uptake rates were monitored for 6 h. The greatest increase in pH levels, from 7.50 to 8.65, occurred in response to $60{\mu}M\;NH_4{^+}$, whereas the largest decrease, from 7.50 to 7.42, was associated with elevated respiration rates. At a pH of 7.5, rates of oxygen evolution were higher (236% saturation) for samples treated with $60{\mu}M\;NH_4{^+}$ than for the control (121% saturation). Ammonium uptake was highest at pH 7.5 and $60{\mu}M\;NH_4{^+}$, with a rate of $0.526{\pm}0.002{\mu}mol\;g^{-1}\;FW\;h^{-1}$, followed in order by the treatments of $pH\;8.2/60{\mu}\;NH_4{^+}$, $pH\;7.5/4{\mu}M\;NH_4{^+}$, and the control ($pH\;8.2/4{\mu}M\;NH_4{^+}$). We speculated that the rates of photosynthesis and $NH_4{^+}$ uptake could be enhanced at a higher ammonium concentration and lower pH because $CO_2$ concentrations were increased through greater photosynthetic activity. Therefore, these findings suggest that the physiology of G. chorda populations can be improved by the interaction of optimized $CO_2$ concentrations and an adequate supply of essential nutrients such as ammonium.

Levels and Related Factors of Airborne Fungi in Microbial and Chemistry Laboratories in Universities (일부 대학교 미생물실험실 및 화학실험실에서의 진균 분포 및 관련인자)

  • Hwang, Sung-Ho;Jo, Hyun-Woo;Park, Dong-Uk;Yoon, Chung-Sik;Ryu, Kyong-Nam;Ha, Kwon-Chul
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.20 no.1
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    • pp.41-46
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    • 2010
  • The purpose of this study is to assess the level of fungi concentration in the university laboratories in Seoul, Korea, and to investigate factors contributing to these concentrations. The samples were taken from three spots in each laboratory; the top of sink, the center of laboratory, and the front of ventilation system, i.e fume hood at the chemical laboratory and clean bench/biosafety cabinet at the microbial laboratory. Air samples were collected using the single-stage Anderson sampler (Quick Take 30) at a flow rate of 28.3 l/min for 5 min on nutrient media in Petri-dishes located on the impactor. Fifty-two air samples were collected from 19 different laboratories (13 microbiology laboratories, 6 chemistry laboratories) in the university, and concentrations of airborne fungi showed no significant difference (p>0.05) between microbiology and chemistry laboratory, and also no significant difference at three locations (sink, center, front of ventilation system) in microbiology and chemistry laboratories. Average concentrations of fungi in 19 laboratories ranged from 7 to 459 cfu/$m^3$, with an overall Geometric Mean of 52 cfu/$m^3$. Airborne fungi concentrations of 6 samples (12 %) exceeded 150 cfu/$m^3$, the guideline of WHO. The ratios of Indoor/Outdoor for airborne fungi ranged from 0.2 to 4.8 (mean = 1.6). Related factors were measured such as relative humidity, temperature, and laboratory area. Temperature and laboratory area showed no significant relations to concentrations of airborne fungi except for relative humidity in the laboratory Concentrations of fungi were significant different (p<0.01) between rainy or cloudy and sunny. However, there was no significant difference between general ventilation and nongeneral ventilation.