• Maxillofacial Plastic and Reconstructive Surgery
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• 제20권4호
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• pp.334-340
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• 1998

Stimulatory effects of epidermal growth factor (EGF) and transforming growth $factor-{\alpha}$($TGF-{\alpha}$) on the growth of squamous cancer cell lines established from human oral cancer tissue with moderate differentiation were studied in vitro. After culturing in serum-free media for 24 hours, growth factors-EGF only, $TGF-{\alpha}$ only and EGF, $TGF-{\alpha}$ together-were added to the media and numbers of cells were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and compared with the control at 96, 144 hours. Each of EGF and $TGF-{\alpha}$ showed statistically significant stimulatory effects on the growth of cells respectively. Dose-dependent relationship of the stimulatory effects were not clearly demonstrated. The effects of EGF were higher than those of $TGF-{\alpha}$ and combinative administration showed higher effects than those of single uses. In conclusion, EGF may play an important and major role in differentiation and growth of human oral squamous cancer cells. $TGF-{\alpha}$, produced from cells activated by EGF, also can stimulate the cell growth and could be an alternative ligand for EGF receptor.

• 대한기계학회논문집
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• 제13권4호
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• pp.563-571
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• 1989

본 연구에서는 이들 무시된 영향을 고려하여 하중작용점에서의 하중분포계수 를 구하여, 이 하중분포계수에 의해 위치에 따라 실제와 가까운 하중을 가하므로서 보다 엄밀한 판의 해석을 할 수 있도록 제시하였다.

• 한국가축번식학회지
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• 제21권2호
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• pp.177-183
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• 1997

The present study examined the effects of epidermal growth factor(EGF) and transforming growth factor-$\alpha$(TGF-$\alpha$) on in vitro maturation of porcine follicular oocytes. Basic medium used TCM-HEPES, and oocytes cultured for 42 hours in vitro. The results obtained are as follows; 1. The nuclear maturation rates of EGF-treated groups(10ng/ml, 75.9% ; 30ng/ml, 69.2% ; 50ng/ml, 67.2% ; 100ng/ml, 71.0%) on the porcine oocytes cultured in medium without pFF in vitro were significantly(P<0.01) higher than those of non-treated group(57.1%). When the oocytes were cultured in media with 10%(v/v) pFF, the nuclear maturation rates of 30ng EGF/ml(77.1%) treated group were significantly(P<0.01) higher than those of non-(59.2%) and EGF-treated groups(10ng/ml, 65.4% ; 50ng/ml, 65.5% ; 100ng/ml, 70.4%). 2. The nuclear maturation rates of 30ng TGF-$\alpha$/ml treated group(71.9%) in media without pFF in vitro were significatnly(P<0.01) higher than those of non-(57.1%) and TGF-$\alpha$ treated groups(10ng/ml, 60.4% ; 50ng/ml, 65.4% ; 100ng/ml, 60.0%). When the oocytes were cultured in media with 10%(v/v) pFF, the nuclear maturation rates of 30 and 50ng TGF-$\alpha$/ml(77.4% and 79.6%) treated groups(10ng/ml, 64.2% ; 100ng/ml, 61.6%). 3. On the effect of EGF(30ng/ml) and/or TGF-$\alpha$(30ng/ml) treated groups in medium without pFF in vitro, the nuclear maturation rates indicated 57.3, 60.4, 75.9 and 79.7% in media with no EGF & TFG-$\alpha$, TGF-$\alpha$ only, EGF only nad EGF＋TGF-$\alpha$ treated groups, respectively. The nuclear maturation rates in medium with EGF only or EGF＋TGF-$\alpha$ were significantly(P<0.01) higher than those non- and TGF-$\alpha$ treated groups. When the oocytes were cultured in media with 10%(v/v) pFF, the nuclear maturation ratesof EGF＋TGF-$\alpha$ treated group(75.9%) were significantly(P<0.01) higher than those of non-(59.2%), TGF-$\alpha$ only (64.2%) and EGF only(69.4%) treated groups.

• 상하수도학회지
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• 제21권6호
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• pp.755-761
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• 2007

Disinfection performance in clearwell is generally measured by CT, which is expressed by $C{\times}T$. C is represented by disinfection concentration, and T is represented by $T_{10}$ which means 90% contact time in clearwell. In order to improve Disinfection performance, augmentation of $T_{10}$ is required. Guide wall has been generally used to improve $T_{10}$ because $T_{10}$ changes according to flow field. In this study, porous media is proposed instead of guide wall, and disinfection performance between guide wall and porous media wall are compared. Flow field and $T_{10}$ in each clearwell are investigated as well. Improved Disinfection performance appear in case of porous media wall compare to guide wall, and best performance occur in porosity factor ${\beta}$ 1e+4.

• International Journal of Contents
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• 제5권3호
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• pp.33-39
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• 2009

This study tested a path model of agenda building examining the relationship among the media, the public and the presidential statements on the issue of drug abuse during the Bush administration's War on Drugs. The results showed that a dynamic and interactive relationship among aforementioned actors. First, the real-world conditions strongly increased both media coverage and presidential statements. Second, the news media and president influenced each other. Third, public concern, however, did not function as a significant factor in agenda-building process in this study. The result of this study indicates that Bush was not utterly a reactive or proactive president in terms of his relationship with the media. That is, news media and the president interacted regarding the drug issues; the relationship between President Bush and the media for drug issues was reciprocal.

• 한국언론정보학보
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• 제81권
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• pp.122-152
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• 2017

언론운동은 정치권력으로부터 독립함과 동시에 언론권력을 해체하고 시민들의 언론 주권을 찾으려는 사회적 운동이다. 언론의 공공성과 독립성, 책임성을 위협하는 가장 큰 요인은 공적 영역에서는 정치권력, 사적 영역에서는 소유주로부터 나오는 언론권력이다. 시민언론운동은 언론의 주권은 시민에게 있으며 이러한 주권을 침해할 우려가 있는 정치권력과 언론 권력을 감시하는 주체로서 시민의 운동성을 강조한다. 이명박ᐨ박근혜 정권에서 시민언론운동은 저항과 투쟁의 시기였다. 시민언론운동은 정치권과의 거버넌스가 완전히 붕괴되었고 언론 전문성을 가진 지식인이 정책 생산을 중심으로 참여하는 정책적 대안의 논의와 제시 방식의 언론시민운동은 설자리를 잃었다. 시민들의 직접 행동보다는 활동가들 중심으로 활동하는 시민언론 단체들의 영향력은 한계를 보였다. 시민언론운동이 언론개혁의 동력을 강화하려면 시민사회, 언론노조, 언론단체, 정치세력 등 사회적 힘의 재구성이 필요하다. 운동의 의제와 활동에 시민들이 주도적으로 참여할 수 있는 다양한 방안도 모색해야 한다. 아울러 언론운동에서 미디어운동으로 외연을 확장할 필요가 있다.

• Environmental Engineering Research
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• 제13권3호
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• pp.141-146
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• 2008

Transport of Escherichia coli ATCC 11105 through porous media was investigated in this study using two sets of column experiments to quantify the attachment-related parameters (sticking efficiency, attachment rate coefficient and filter factor). The first set of experiments was performed in quartz sand under different ionic strength conditions (1, 20, 100, 200 mM) while the second experiments were carried out in quartz sand mixed with metal oxyhydroxide-coated sand (0, 5, 10, 25%). The breakthrough curves of bacteria were obtained by monitoring effluent, and then bacterial mass recovery and attachment-related parameters were quantified from these curves. The first experiments showed that the mass recoveries were in the range of 13.3 to 64.7%, decreasing with increasing ionic strength. In the second experiments, the mass recoveries were in the range of 15.0 to 43.4%, decreasing with increasing coated sand content. The analysis indicated that the sticking efficiency, attachment rate coefficient and filter factor increased with increasing ionic strength and coated sand content. The value of filter factor in the first experiments ranged from 1.45 e-2 to 6.72 e-2 1/cm while in the second experiments it ranged from 2.78 e-2 to 6.32 e-2 1/cm. Our filter factor values are one order of magnitude lower than those from other studies. This discrepancy can be attributed to the size of sand used in the experiment. The analysis demonstrated that the travel distance of bacteria estimated using the filter factor can be varied greatly depending on the solution chemistry and charge heterogeneity of porous media.

• Clinical and Experimental Reproductive Medicine
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• 제49권2호
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• pp.149-158
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• 2022

Objective: Optimizing culture media for the incubation of immature oocytes is a vital strategy to increase the oocyte maturation rate during in vitro maturation (IVM) programs. This study evaluated the IVM and fertilization rates of human germinal vesicle (GV) and metaphase I (MI) oocytes using two different maturation media (commercial and homemade) with or without growth differentiation factor 9-β (GDF9-β). supplementation. Methods: Immature oocytes from intracytoplasmic sperm injection (ICSI) cycles were collected and assigned to one of two IVM culture media (commercial or homemade; cleavage-stage base). After maturation, MII oocytes were examined under an inverted microscope for the presence of the polar body, zona pellucida (ZP) birefringence, and meiotic spindle (MS) visualization after maturation in four conditions (commercial or homemade medium, with or without GDF9-β. ICSI was done for matured oocytes, and fertilization was confirmed by the visualization of two distinct pronuclei and two polar bodies. Results: No significant differences were found between the two culture media in terms of the time and rate of oocyte maturation or the rate of fertilization (p>0.05). Growth factor supplementation increased the 24-hour maturation rate for both GV and MI oocytes only in homemade medium. The maturation rate after 24 hours was higher for MI oocytes (p<0.05). Similar results were observed for MS visualization and ZP structure in both types of media (p>0.05). Conclusion: Higher rates of oocyte maturation and fertilization were observed after application of homemade medium supplemented with GDF9-β. Therefore, this combination may be recommended as an alternative for clinical IVM programs.

• Toxicological Research
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• 제11권1호
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• pp.117-125
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• 1995

In order to examine the mechanism of asbestos clastogenicity, CHO cells were treated with chrysotile and crocidolite. Crocidolite and chrysotile were able to induce lipid peroxidation in a dose dependent manner. Ultrafiltrate of culture media from CHO cells treated with chrysotile/crocidolite induced sister chromatid exchange in CHO cells. Ultrafiltrate of culture media from CHO cells treated with chrysotile induced chromosome aberration but it was not statistically significant. Simultaneous treatment of 3-Aminobenzamide (3-AB) or cytosine arabinoside (Ara C) with crocidolite had no effect on the frequency of chromosome aberration by crocidolite whetease posttreatment of caffeine significantly increased the chromosomel aberration by crocidolite. This indicated that DNA damage by asbestos took place at late stage of cell cycle. The results suggested that the ultrafiltrate of media contained clastogenic factor (CF) and lipid peroxidation might be involved in the formation of CF.

• International journal of advanced smart convergence
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• 제13권1호
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• pp.129-139
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• 2024

In digital marketing, the strategic use of emojis in social media advertising, particularly on the Xiaohongshu app, significantly influences consumer acceptance and purchase behavior. This study examines the impact of emoji-laden advertisements and the role of brand metaphorical warmth on consumer perceptions. Employing a tailored questionnaire, the research explores how emojis affect brand advertisement reception, filling a gap in empirical research on emoji advertising effectiveness. Findings indicate that emojis, when used judiciously, enhance consumer acceptance and contribute to a positive brand perception. However, excessive use may undermine trust. Brand metaphorical warmth emerges as a crucial factor, suggesting that emojis can effectively convey warmth, fostering a deeper emotional connection with consumers. These insights offer practical implications for refining social media marketing strategies, advocating for a balanced approach to emoji usage in advertisements to optimize engagement and influence consumer behavior.