• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.875-880
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• 2015

Mediator 19 (Med19) is a component of the mediator complex which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II. Accumulating evidence has shown that Med19 plays important roles in cancer cell proliferation and tumorigenesis. The involvement of Med19 in sensitivity to the chemotherapeutic agent cisplatin was here investigated. We employed RNA interference to reduce Med19 expression in human non-small cell lung cancer (NSCLC) cell lines and analyzed their phenotypic changes. The results showed that after Med19 siRNA transfection, expression of Med19 mRNA and protein was dramatically reduced (p<0.05). Meanwhile, impaired growth potential, arrested cell cycle at G0/G1 phase and enhanced sensitivity to cisplatin were exhibited. Apoptosis and caspase-3 activity were increased when cells were exposed to Med19 siRNA and/or cisplatin. The present findings suggest that Med19 facilitates tumorigenic properties of NSCLC cells and knockdown of Med19 may be a rational therapeutic tool for lung cancer cisplatin sensitization.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.3695-3700
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• 2012

Mammalian mediator (MED) is a multi-protein coactivator that has been identified by several research goups. The involvement of the MED complex subunit 19 (MED 19) in the metastasis of lung adenocarcinoma cell line (H1299), which expresses the MED 19 subunit, was here investigated. When MED 19 expression was decreased by RNA interference H1299 cells demonstrated reduced clone formation, arrest in the S phase of the cell cycle, and lowered metastatic capacity. Thus, MED 19 appears to play important roles in the biological behavior of non-small cell lung carcinoma cells. These findings may be important for the development of novel lung carcinoma treatments.

• BMB Reports
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• v.44 no.8
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• pp.547-552
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• 2011

MED19 is a member of the Mediator that plays a key role in the activation and repression of signal transduction or the regulation of transcription in carcinomas. To tested the functional role of MED19 in human prostate cancer, we downregulated MED19 expression in prostate cancer cells (PC-3 and DU145) by lentivirus-mediated short hairpin (shRNA), and analyzed the effect of inhibition of MED19 on prostate cancer cell proliferation and tumorigenesis. The in vitro prostate cancer cell proliferation, colony formation, and in vivo tumor growth in nude mice xenografts was significantly reduced after the downregulation of MED19. Knockdown of MED19 caused S-phase arrest and induced apoptosis via modulation of Bid and Caspase 7. It was suggested that MED19 serves as a novel proliferation regulator that promotes growth of prostate cancer cells.

• Proceedings of the Ginseng society Conference
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• 2005.05a
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• pp.19-19
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• 2005

• 대한임상약리학회:학술대회논문집
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• 1998.12a
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• pp.64-64
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• 1998

• 대한임상약리학회:학술대회논문집
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• 1998.12a
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• pp.62-62
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• 1998

• Communications for Statistical Applications and Methods
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• v.19 no.1
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• pp.33-45
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• 2012

The primary interest of drug development studies is to estimate the smallest dose that shows a significant difference from the zero-dose control. The smallest dose is called the Minimum Effective dose(MED). In this paper, we suggest a nonparametric procedure to simultaneously find the MED of each group based on placements. The Monte Carlo simulation is adapted to estimate the power and the family-wise error rate(FWE) of the new procedures with those of discussed nonparametric tests to find MED.

• Journal of Technologic Dentistry
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• v.35 no.4
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• pp.287-293
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• 2013

Purpose: This study is to provide basic data of the dental acrylic denture base resin in the mechanical property difference investigation according to the monomer composition weight ratio of the acrylic denture base resin. Methods: The monomer composition of the acrylic denture base resin and weight ratio makes the different specimen. It measured the mechanical property with the specimens through Hardness Test, Tensile Test, Flexural Test, Flexural Modulus, FT-IR Test. Results: The control group Vertex was 18.4 Hv and the experimental group MED was 14.46~19.07Hv in the hardness test. Vertex was 364N, MED-3 was lowest in the tensile strength test and the Head of a family cursor declination was big. The result declination of the experimental specimens showed. Vertex and MED-2 was the highestest in the flexural test and after coming MED-6, MED-5, MED-1, MED-3, MED-4. Vertex and MED-2, as to a spectrum for $500{\sim}1800cm^{-1}$ peak can show the excellent degree of polymerization in the FT-IR Test. Conclusion: The ideal weight ratio of the monomer of the acrylic denture base resin of which the mechanical property is the highestest was MMA 100g, EDGMA 5g, DMA 0.2g, of MED-2.

• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2003.10a
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• pp.41-41
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• 2003

White spot syndrome virus (WSSV) is the causative agent of a disease that has led to severe mortalities of cultured shrimps in Korea and many other countries. Since 1993, massive mortalities due to the viral infection have also occurred in the penaeid shrimps cultured in Korea. WSSV is a large, circular, double stranded (ds) DNA virus and an enveloped, ellipsoid virus with a rod-shaped nucleocapsid with flat ends. In order to identify the characteristics of this Korean isolate of WSSV, the genes for four virion proteins, VP15, VP19, VP28 and VP35 were cloned and their sequences were compared with the available pool of WSSV gene sequences in the GenBank/EMBL databases. From these comparisons, we confirm the occurrence of WSSV in Korea and deduce that, VP15, VP28 and VP35 genes are identically conserved among the Korean isolate and geographically different foreign isolates, but VP19 amino acid sequences of the Korean WSSV isolates changed valine of the foreign isolates into aspartate. (omitted)

• Archives of Plastic Surgery
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• v.46 no.5
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• pp.462-469
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• 2019

Background Incisional hernia is a common complication following visceral organ transplantation. Transplant patients are at increased risk of primary and recurrent hernias due to chronic immune suppression and large incisions. We conducted a retrospective review of patients with a history of liver or kidney transplantation who underwent hernia repair to analyze outcomes and hernia recurrence. Methods This is a single center, retrospective review of 19 patients who received kidney and/or liver transplantation prior to presenting with an incisional hernia from 2011 to 2017. All hernias were repaired with open component separation technique (CST) with biologic mesh underlay. Results The mean age of patients was $61.0{\pm}8.3years\;old$, with a mean body mass index of $28.4{\pm}4.8kg/m^2$, 15 males (78.9%), and four females (21.1%). There were seven kidney, 11 liver, and one combined liver and kidney transplant patients. The most common comorbidities were hypertension (16 patients, 84.2%), diabetes (9 patients, 47.4%), and tobacco use (8 patients, 42.1%). Complications occurred in six patients (31.6%) including hematoma (1/19), abscess (1/19), seroma (2/19), and hernia recurrence (3/19) at mean follow-up of $28.7{\pm}22.8months$. With the exception of two patients with incomplete follow-up, all patients healed at a median time of 27 days. Conclusions This small, retrospective series of complex open CST in transplant patients shows acceptable rates of long-term hernia recurrence and healing. By using a multidisciplinary approach for abdominal wall reconstruction, we believe that modified open CST with biologic mesh is a safe and effective technique in the transplant population with complex abdominal hernias.