• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.363-370
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• 2014

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.

• Korean Journal of Poultry Science
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• v.21 no.4
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• pp.239-247
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• 1994

A study was conducted to investigate the effects of the addition of varied levels of NaCi and phosphates on the physicochernical properties of the breast meat of the spent layers(2 \pm 0.2 kg) which were stabilized for over 24 h before slaughter. Within 1 h after slaughter, breast meats were removed and treated with NaGl(0, 1, 2, 3%) and phosphates(0.25% and 0.5%) using a hot-salted method. The breast meat was stored at 4 \pm $1^{\circ}C$ for 3 d. The results obtained were summarized as follows. 1. The pH values of salt-treated groups were significantly higher than that of the control(P<0.05) ; the higher the salt level, the higher the pH. The pH values were significantly increased in both control and treatment groups during storage(P<0.05). Among salt-treated groups, the 0.5% phosphates level showed significantly high pH(P<0.05) compared to other levels of salt groups. 2. The moisture contents were significantly lower in all salt4reated groups than the control(P<0.05), and showed a negative relationship with the levels of salt. It decreased in control group gradually as the storage period extended, but not significantly changed in salt-treated groups. 3. The shear force values in salt-treated groups were lower than that of the control and showed a negative relationship with salt levels. At a constant level of NaCI, the shear force value was higher in 0.25% phosphates level than in 0.5% level. It decreased in both control and salt-treated groups during storage. 4. The salt treatments tended to increase the sodium content proportionately. The sodium content decreased in both control and salt treatment groups during storage(P<0.05). In addition, the combination of high levels of NaCl and phosphates rather than those of low levels of NaCI and phosphates resulted in elevated levels of sodium. 5. The phosphorus contents in salt-treated groups were higher than that of control. Between 0.5% and 0.25% phosphates levels this value showed significant difference(P<0.05). Its contents in both control and treatment groups were significantly decreased during storage (P<0.05).

• Food Science of Animal Resources
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• v.30 no.2
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• pp.298-304
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• 2010

This study was performed to evaluate functional and textural properties of low-salt pork meat products treated with transglutaminase (TG) using sodium polyphosphate (STPP) and sodium pyrophosphate (SPP). In experiment 1, lightness and yellowness decreased (p>0.05), but moisture content and cooking yield (%) increased with increased salt level (p<0.05). Based on these results, at least 1.0% salt was required to manufacture comminuted pork meat without quality defects. The effect of STPP and SPP with TG in low-salt (1%) pork comminuted shoulder meat products was evaluated in experiment 2. pH values increased with the addition of phosphate (p<0.05), with pH values in treatments containing TG and PP alone or in combination being higher than those with STPP alone (p<0.05). Cooking yield of treatments with TG and phosphates was higher than those without phosphates, but lower than CTL (1.5% salt and 0.4% STPP; p<0.05). Springiness of pork comminuted meat containing SPP was higher than those of CTL (p<0.05). These results indicate that low-salt meat products can be produced by the combination of TG and phosphate either alone or in combination to maintain cooking yield and textural properties.

• Food Science of Animal Resources
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• v.33 no.1
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• pp.96-102
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• 2013

The possibility of instrumental differentiation of duck breast meat treated with different processing and storage conditions was investigated for industrial application. Duck breast meats, which were 1) refrigerated (fresh) after slaughter, 2) fresh but applied the torching process for the removal of remaining feathers (fresh-torched), and 3) frozen and thawed (frozen-thawed), were prepared and the torrymeter value and other quality factors were assessed. The torrymeter values of both duck breast meat and skin showed the lowest in frozen-thawed sample during the whole storage period. The drip loss of frozen-thawed sample was higher than those of fresh or fresh-torched ones. The number of total aerobic bacteria was lower in fresh-torched than fresh but both were not different from frozen-thawed at day 1 while no difference found thereafter. Sensory score of frozen-thawed sample was the lowest. The correlation analysis among the torrymeter value and quality factors of duck breast meat revealed that the torrymeter value is closely related with the total aerobic bacterial number, lipid oxidation, drip loss, and storage period but not with color. The results indicate that once the duck breast meat was frozen then thawed, drip loss and sensory quality can be affected and the torrymeter value can differentiate frozen-thawed from fresh and freshtorched duck breast meat.

• Journal of the Korean Society of Food Culture
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• v.11 no.4
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• pp.439-454
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• 1996

The present study investigated Bul-Chun-Wi Sacrificial Ritual (sacrificial service which has been handed down from generation to generation to worship the family ancestors in the period of $1400{\sim}1800$) and foods for the sacrificial service among fourteen head families in Andong area. The findings are as follows; 1. In Bul-Chun-Wi Sacrificial Rituals, family shrine has been maintained in good shape, and the table, dishes, and foods used in the rituals have not been changed so much until these days. 2. While vegetable soup is widely used as soup, one family uses the seaweed soup, the other soup mixed with meat, fish, and vegetable. Specially soybean-powdered soup, which is the distinguishable food in Andong area, has been used. 3. As a basic Ddock, mainly Si-Ru-Ddock(a steamed rice cake), piled up to 13-15 stacks, is used. Additional 7-9 kinds of Ddock are placed on top of the basic Ddock. 4. For grilled-meat food(Geuck), eight families use the raw meat, and one family uses the half-cooked meat. Recently, five families have used the cooked meat. Mostly used ones are meat-Geuck, fish-Geuck, chicken-Geuck, and the Geuck are not served one by one. Instead the Geuck are stacked in one dish designed for Geuck in order of meats from poultry, animal, fish, and shell. As the sub-dishes for rice, raw and cooked Geuck are used. 5. The number of stew (Tang) are 3 to 6 and 5 stews is the most popular. Commonly used stews are meat stew, fish stew, chicken stew, vegetable stew, blood stew, and organs stew. For the vegetable stew, buckwheat gel can be used. 6. As the fruit, chinese date, pear, nut and dried persimmons are the basic ones. The even number of 6 or 8 colorful fruits are used, while the odd number of 7 or 9 colorful fruits are used in three head families. 7. As Sik-Hae which is a drink and made from fermented rice, rice Sik-Hae or fish Sik-Hae has been necessarily used. 8. As raw meat dish, the liver of cow or meat is used. As a wrapping materials, the reticulum of a ruminant, green seaweed or thinly fried egg can be used.

• Journal of the Korean Society of Food Culture
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• v.11 no.5
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• pp.699-710
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• 1996

The sacrificial foods using at 88 villages in Andong area where the village sacrificial rituals are performed, were investigated in 1994. The finding are as follows; 1. Since the village sacrificial rituals keep ancientry, white Baeksulki without any ingredients is used as Ddock (a steamed rice cake) in 74 villages, and in 5 villages the Baeksulki was served just in Siru (a tool used to prepare Ddock). The type of meat serving in rituals has been changing from large animal to small animal and the type of light from bowl-light to candle. 2. Though the ritual observes Confucianism style, memorial address was offered in 34 villages and only in 8 villages, liquor was served 3 times and the address was offered. In 60 villages, liquor was served only once, and calling village god, bowing twice, and burning memorial address paper were performed in order. Burning paper has been meaningful in rituals. 3. Tang (a stew) was served in 21 villages, and in 18 villages even Gook (a soup) was not used. The numbers of village which do not use cooked rice and Ddock are 14, respectively. 4. Fruits are essential in rituals foods. In 63 villages, 3 kinds of fruits are served and this is contrasted with even number of fruits in Bul-Chun-Wi Rituals. 5. In 25 villages, fish and meat are not served. As meat, chicken in 19 village are served. Meat Sikhae (a fermented rice beverage) prepared with meats or fish are served in 5 villages. Slice of dried meat is served in 73 villages commonly. 6. In gender of god for village shrine, woman in 43 villages, couple in 17 villages, man in 9 villages, mountain god in 2 villages, villages tree, etc were enshrined. In 52 villages liquor is served in rituals (liquor is used in 58 villages including serving god), in 28 villages sweet liquor (Gamju) is served for god (sweet liquor is used in 46 villages), and liquor, water, or sweet liquor is just displayed on table but not served in 8 villages. Incensing is found to be not common in village ritual, only in two villages it is performed. The most appropriate rituals food is thought to be pig for mountain god. Home dishes can not be used in village ritual and spoon is not used and chopstick is prepared from wild plant. Meat and fish are used in the raw.

• Korean Journal of Community Nutrition
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• v.9 no.2
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• pp.161-172
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• 2004

The dietary habits of middle-aged obese, overweight and normal subjects were evaluated for the purpose of providing the background information of the degenerative disease control. A survey was conducted with 293 subjects (121 males and 172 females) between the ages 40-64 and they were divided into three groups of normal (BMI 18.5-22.9), overweight (BMI 23-24.9) and obese ($BMI\geq25$) based on the criteria of Korean Obesity Association (2000). The mean age of the subjects was $52.0\pm7.3(male)\;and\;51.9\pm6.9$ (female). Socioeconomic levels of subjects belong to the mid-upper class, since 71.9% of the males and 39.5% of the females received above a college education. More than 40% (44.6%) of males and 22.1% of females were obese, and educational background did not affect the obesity rate in males, whereas in females those who received lower education had a higher rate of obesity (p ＜ 0.001). The subjects had a similar degree of drinking to the national average value. but had a lower degree of smoking. The obese group had the higher rate of drinking (p ＜ 0.01) and smoking (p ＜ 0.01). The annual increase in weight, more than 4 kg, was 8.8% for normal group and 30.9% for obese group. However, there were no differences in the mean annual increase in weight among the three groups. In females there were greater tendencies of weight changes in obese group (p ＜ 0.05). About 44.9% of subjects responded that they were exercising regularly, and the obese group appeared to put into practice less than the other groups and to use more strenuous exercise. There was the greater rate of skipping dinner in the obese female group (p ＜ 0.05). The unbalanced dietary pattern was found more with normal group than the other groups (p ＜ 0.01). The food consumption frequency by food groups was not different among the groups. The obese group consumed less frequently meats, vegetables and fruits and had higher frequency in fish, legumes and their products, instant or fast food. In all subjects the higher rate of obesity was found with males than females and with those of heavier smoking and drinking, and in females the higher rate of obesity was found with lower education levels, skipping meals and having an unbalanced diet. In middle-aged subjects of this study with higher educational and socioeconomic background factors contributing to the effects of obesity may include smoking, drinking, educational background, skipping meals or unbalanced dietary pattern. The predictable characteristics for the development of obesity can be defined as sudden fluctuation of weights, exercise regularity and intakes of vegetables and fruits.

• Korean Journal of Community Nutrition
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• v.9 no.3
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• pp.263-273
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• 2004

The purpose of this study was to investigate the pattern of individual fatty acid intake and to compare serum lipid levels and total serum fatty acid composition of high school students in Seoul (total : 234 ; male : 91 ; female : 143) In serum lipid levels, total cholesterol (Chol.), HDL-Chol. and LDL-Chol. levels of female students were significantly higher than those of male students and there was no significant difference between High Fish ＆ Low Meat intake (HFLM) and Low Fish ＆ High Meat intake (LFHM) groups. The average fat intake was 22-25 energy % of total subjects and especially, that of LFHM group was 29%, which were over the recommendation level. Although the average P/M/S ratio of dietary fat was 1.1/1.2/1.0, the average range of $\omega6/\omega3$ fatty acid ratio of dietary fat was found to be 17.9-20.7, which was far beyond the suggested range, 4-10. The average intake of cholesterol of total subjects was 360mg. LFHM group had more meats and beverages such as carbonated drinks and tended to have less beans, vegetables and mushrooms. In addition, LFHM group had more energy and fat intake than those of HFLM group, the P/S ratio of dietary fat (0.73) was lower than the recommended ratio. Serum C16 : 0 composition of LFHM group was significantly higher than that of HFLM group, and EPA and DHA composition of HFLM was significantly lower than that of LFHM. Therefore, in HFLM group, the P/S ratio of serum fatty acids was significantly higher and the $\omega6/\omega3$ ratio was lower. Dietary C18 : 0 was negatively correlated with serum EPA and DHA composition. Individual PUFA intake was negatively correlated with serum C16 : 0 and sum of SFA, and positively correlated with serum C18 : $2\omega6(LA)\;,\;sum\;o;\omega6$ and sum of PUFA. Serum C18 : 1, C18 : $3\omega3\; and\; C20\;:\;4\omega6$ (AA) compositions were not correlated with dietary fatty acid. Only serum triglyceride (TG) levels were significantly correlated with serum fatty acid compositions. Sum of SFA, C14 : 0, C16 : 0, sum of MUFA and C18 : 1 compositions were positively correlated with serum TG levels, but LA, AA, sum of PUFA and P/S ratio were negatively correlated with it.

• Journal of Nutrition and Health
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• v.39 no.2
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• pp.184-191
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• 2006

This study investigated parents' attitude about children's unbalanced diet. The subjects were 1,309 parents that their children attended at kindergarten or child care center in Kyoung-Ki province. The order of dislikes in 'vegetables and fruits' was as follows: all kinds of vegetables (56.6%), beans (17.6%), fruits (5.2%). In 'meat, fish, poultry', the order of dislikes was meats (38.2%), fishes (21.8%), milk (16.4%), eggs (10.0%). In 'cerelas', the order of dislikes was rice (31.0%), rice cakes (14.3%), noodles (11.9%). The several rationalizations for dislikes are 'taste' (19.1 %), 'food habit from younger age' (18.4%), 'lack of chances for trying new food' (16.0%) etc. To compared children's rationalizations for dislikes to their age group, the percentage of 'lack of chances for trying new food', 'strange food shape', 'lack of nutrition education' was significantly higher in 'below 5 years old' (<5) group than other groups (<6, <7) and 'sweety food' was significantly higher in 'below 7 years old' (<7) group than other groups (<6, <7)(p <0.05). In parents' attitude about children's unbalanced dietary habit, subjects answered that they try to make children understand to eat dislike food (73.9%), develop new recipe for children (14.0%), neglect children's food habit (6.2%). To compared parents' attitude for children's unbalanced diet by parents age, the percentage of 'try to make children understand to eat dislike food' was significantly higher in 'below 30 years old' (<30) group than other groups ($30{\sim}35,\;35{\sim}39,\;{\geq}40$) and 'developing new recipe' was significantly higher in 'over 40 years old' (${\geq}40$) group than other groups ($<30,\;30{\sim}35,\;35{\sim}39$)(p < 0.05). To compared parents' attitude for children's unbalanced diet by parents' occupation, the percentage of 'neglecting' was higher in employed group and 'developing new recipe' was higher in unemployed group than the other group. According to the results of the survey, it is necessary to make new educational materials for employed parents and young children and develop new recipes to use various kind of foods instead of forcing unpleasant foods on the children for the sake of unbalanced diet.

• Journal of Nutrition and Health
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• v.38 no.10
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• pp.856-863
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• 2005

Dietary Variety Score (DVS) or Nutrient Adequacy Ratio (NAR) offers a way of comparing eating habits across populations and across countries. This study compared nutrients intakes, food intakes, DVS, and NAR between Korean and US adults using the large national survey data. 4662 Korean adults (men: 2201, women: 2461) aged 20-49 years were selected from the 'Korean National Health and Nutrition Survey,2001' and 4199 US adults (men: 2127, women: 2072) aged 20-49 years were selected from the 'US National Health and Nutrition Examination Survey (NHANES IV,1999-2002)'. The total plant food intakes were high in Korean adults, but the total animal food intakes were high in US adults. Intakes of legumes and nuts, vegetables and mushrooms were higher in Korean than in US adults. On the other hand, intakes of meats, milk and dairy products of US adults were higher than Korean. Beverage intakes were 8 - 10 times higher in US adults than in Korean. The mean DVS of Korean men and women were higher than US men and women. Intakes of fat, calcium and vitamin $B\_{2}$ of US adults were 1.5 - 2 times higher than those of Korean, which could probably related to larger consumption by US adults on milk and dairy product. While the intakes of carbohydrate and vitamin C of the Korean were higher than the US adults, which could presumably related to higher grain and vegetable consumption. Percent energy intakes from carbohydrate, protein, and fat were $64.1\%$, $15.8\%$, and $20.1\%$ for Korean men, $52.4\%$, $15.0\%$, and $32.6\%$ for US men, $65.9\%$, $14.9\%$, $19.2\%$ for Korean women, and $52.1\%$, $14.7\%$, $33.2\%$ for US women respectively, which showed higher $\%$energy from carbohydrate in Korean and higher $\%$energy from fat in US adults. Most nutrient intakes except energy and vitamin C expressed as $\%$RDA were higher in US than in Korean adults. Mean Adequacy Ratio (MAR) of US men and Korean men were both 0.85, while the MAR was 0.81 for Korean women,0.79 for US women. The Korean women's diet showed more diverse and adequate than the US women's diet.