• Title/Summary/Keyword: Measured Frequency

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Comparison of Ultrasound Histogram in Liver, Kidney and Spleen in Beagle Dogs (비글견에 있어서 간, 신장 및 비장의 초음파 히스토그램 비교)

  • Lee Kichang;Jung Joohyun;Oh Sunkyoung;Jeong Yucheol;Lim Changyun;Yoon Junghee;Choi Mincheol
    • Journal of Veterinary Clinics
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    • v.22 no.3
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    • pp.186-189
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    • 2005
  • For the assessment of the clinical application of histogram on internal parenchymal organs, ultrasonography with a multi-frequency transducer was taken. We scanned in the region of right cranial abdomen for both liver and right kidney, and left cranial abdomen for liver, spleen and left kidney in 9 normal Beagle dogs. The data from histogram examined in a region of interest centered on each picture element of B-mode images at the same depth were compared among liver, renal cortex, spleen, cortex and medulla of each kidney. The right renal cortex showed significantly lower echogenicity than parenchyma of liver by $15{\%}$. Spleen was more echogenic than the cortex of the left kidney by $23{\%}$, and liver was more echogenic than the left renal cortex by $30{\%}$. Renal cortex was more echogenic than medulla by $47{\%}$ and $65{\%}$ on the right and left side, respectively (p<0.05). The mean (${\pm}SD$) values calculated echogenicity were $46.2{\pm}12.3\;(95\%$ confidential interval (CI), 41.0 to 55.0) and $53.4{\pm}12.1\;(95\%$ CI, 47.0 to 55.1) in in the right renal cortex and liver parenchyma, $65.0{\pm}11.8\;(95\%$ CI, 57.9 to 71.0) and $51.0{\pm}16.9\;(95\%$ CI, 42.8 to 54.1) in splenic parenchyma and renal cortex. And the mean values calculated echogenicity were $65.0{\pm}10.15\;(95\%$ CI, 60.1 to 71.5) and $52.0{\pm}9.4\;(95\$ CI, 43.8 to 60.3) in liver parenchyma and the left renal cortex, $54.5{\pm}18.3\;(95\%$ CI, 40.1 to 62.8) and $35.0{\pm}16.2\;(95\%$ CI, 24.2 to 43.6) in the left renal cortex and medulla. And the mean values calculated echogenicity were $55.0{\pm}14.4\;(95\%$ CI, 47.3 to 61.7) and $40.0{\pm}13.2\;(95\%$ CI, 34.3 to 46.7) in the right renal cortex and medulla, respectively. In addition, the echogenicity ratios were $0.86{\pm}0.11$ between the right renal cortex and liver parenchyma, $1.37{\pm}0.47$ between spleenic parenchyma and the left renal cortex, $1.30{\pm}0.19$ between liver parenchyma and the left renal cortex. All the values measured showed significant different (p<0.05). Ultrasound histogram is simple, useful and feasible to evaluate the sonographic architecture of the internal organs such as liver, spleen and kidney, quantitatively.

Studies on- Electrocardiogram of the Normal Korean Native Goat 1. Standard Limb Leads and Unipolar Limb Leads (정상적인 한국 흑염소의 심전도에 관한 연구 1. 표전지유도와 단극지유도)

  • 최인혁;김선기;김추철;최인방;김남수
    • Journal of Veterinary Clinics
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    • v.14 no.2
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    • pp.319-337
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    • 1997
  • The electrocardiographic (ECG) parameters in the normal Korean black goat have been measured with a 3-channel Electrocardiograph and computed, analyzed. ECG in 243 black goats were made with the limb leads (I, II, III, aVR, aVL, aVF), that were recorded conduction parameters of wave and interval, and were analyzed as to shape and amplitude of the P and T waves and the components of the QRS complex. Heart rate were recorded by the ECG which were a mean of 106.1$\pm $21.8 beats/min. Average conduction times in the PR, the QRS complex and the QTc interval were recorded 103.9$\pm $34.9 msec., 58.3$\pm $23.2 msec. and 302.6$\pm $67.8 msec., in the P and T wave duration recorded 24.8$\pm $6.4 msec. and 51.7$\pm $10.8 msec. respectively. The shape of wave in each leads were observed various types, and any spacial wave type appeared the highest frequency in each lead that ware shown less than 60%, and these frequent rate and average amplitudes as fallow: 1. In P waver the frequent rate and average amplitudes of the positive type showed in leads I, II and aVL that were 54.8% (93.0$\pm $ 33.2 $\mu $V), 50.5% (90.1$\pm $30.5 $\mu $V) and 41.7% (58.5$\pm $ 31.1 $\mu $V). Average amplitude of the negative type showed the frequent rate of 49.8% in lead aVE which was -77.6$\pm$ 25.2 $\mu $V. Biphasic type in leads III and aVF were 46.1% (108.4 $\mu $V, -90.2 $\mu $V.) and 45.7% (137.4 $\mu $V, -105.4 $\mu $V.), and amplitudes between positive and negative of it were significant difference. 2. The highest amplitudes of the QRS complex in all leads were 534.8$\pm $ 232.3 $\mu $V of lead II. The frequent rate and amplitudes of the R wave type in the I, II, III and aVF were 30.2% (277.8 $\pm $131.3 $\mu $V), 45.1% (393.1$\pm $114.2 $\mu $V), 48.5% (349.3$\pm $178.3 $\mu $V) and 54.9% (334.4$\pm $129.7 $\mu $V), and QS ways type in the lead aVL was 49.5% (359.2$\pm $195.5$\mu $V), and RS and QS wave types in the lead a VR were 43.3% (312.4 $\mu $V, -212.7 $\mu $V.) and 41.1% (399.2$\pm $92.2 $\mu $V), respectively. 3. In T wave, the frequent rata and amplitudes of the positive type in the leads I, II, III and aVF were 44.6% (207.9$\pm $ 97.1 $\mu $V), 41.6% (245.1$\pm $92.1 $\mu $V), 46.9% (189.8$\pm $ 82.7 $\mu $V) and 53.0% (195.4197.8 $\mu $V), and the negative in the lead aVR was 41.2% (-230.7$\pm$ 103.1 $\mu $V), respectively. The positive and negative types in the lead aVe appeared with same frequent rate of 43.2%.4. Frontal plane vectors for P, QRS, and T were found to lie at 38.1$\pm $ 21.5, 142.0$\pm $ 57.2, and 117.2$\pm $ 63.9 degrees, respectively. These results in ECG of goats may be served to the limited purposes as to conduction parameters, arrhythmias except abnormal ECG because of waveforms, amplitudes and electrical axis of it were variability.

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Changes of Lens Morphology and TBUT by Dehydration of Soft Contact Lens (소프트콘택트렌즈 건조로 인한 렌즈 형태 및 TBUT의 변화)

  • Park, Mijung;Lee, Yu-Na;Kang, Kyu Eun;Lee, Min Ha
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.2
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    • pp.1-7
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    • 2008
  • Purpose: This study was performed to evaluate the changes of lens morphology and tear stability during wearing soft contact lenses (SCLs) which were kept in drying condition like dry eye or became to be dried due to heedless care. Method: SCLs having different water content, thickness or material were rehydrated after being dehydrated artificially 2 or 4 times, and estimated their diameter and radius. Furthermore, the changes of tear film break-up time (TBUT) during SCL wearing were also measured. Result: Due to the dryness, the diameter of both 70% water content SCL and 59% water content SCL decreased, but the decrement was larger in 59% water content SCL. The more 59% water content SCL was dehydrated, the more its radius changed. However, the radius of 70% water content SCL did not change by 2 times dehydration and increased greatly by 4 times dehydration. The reduction of diameter of -1.00 D SCL was greater than that of -9.00 D SCL. Moreover, the radius of -1.00 D SCL increased depending on the frequency of dehydration but that of -9.00 D SCL did not changed. The diameter and radius changes of lotrafilcon B, silicone hydrogel lens, were less than those of hilafilcon B, copolymer of HEMA and N-vinyl pyrrolidone. TBUT during wearing SCLs decreased by wearing dehydrated SCLs. Conclusion: The diameter and radius of dehydrated SCLs as well as TBUT during wearing them were changed in spite of rehydration, which would be the important cause of uncomfortable feeling when people wore dehydrated SCL. The changes of SCL morphology and TBUT differed according to the water content, lens thickness and material.

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Comparative Study on Social Support and Perceived Health between Obese Women and Normal Weight Women (비만여성과 정상체중 여성의 사회적지지 및 건강지각의 비교)

  • Kim, Jeong-Ah;Wang, Myoung-Ja
    • Research in Community and Public Health Nursing
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    • v.15 no.4
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    • pp.587-599
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    • 2004
  • Purpose: The purpose of this study is to compare abdomen-fat rate, life style and social-support between normal weight women and obese women. Method: 304 women objectives from their 30 to 59 years of age were selected living in Je-chon city, Chung-Buck province and their height and weight were measured from April 1st to June 30th, 2003. Data were classified into low-weight group ($18.5kg/m^2$), normal-weight group ($18.5{\sim}22.9kg/m^2$), over weight group ($23{\sim}24.9kg/m^2$), and obese group ($25kg/m^2$) following the Korean Conference of Obesity, 2001. in which 119 people in the normal weight group and 91 people in the obese group, i.e. total 210 people were analyzed in sequence. Using SPSS Win 10.1 Program, frequency and percentile, and by ANOVA, $X^2-test$ and t-test were treated. Results: The average age of obese women was 46.68 distributing 40.7% of forties and 39.6% of fifties while normal-weight women were average 41.73-year old distributing 53.8% of forties and 34.5% of thirties, which revealed aged in obese women. The body fat rate of obese women averaged $37.52{\pm}4.17%$, in which 98.9% of obese women and 21.0% of normal weight women with a more than 30% of body-fat rate resulted in a higher body-fat rate in obese women. The waists of obese women averaged $88.37{\pm}8.22cm$, in which more than 85cm showed in obese women of 68.2% and normal weight women of 7.6% indicating a higher waist-fat rate in obese women. The abdomen-fat rate of more than 0.85 of waist vs hip-fat showed 74.7% in obese women and 58.4% in normal weight women, indicating a higher abdomen-fat rate in obese women. Obese women and normal weight women showed significant differences in education level, number of children, religion, menstrual status, and mother's weight. Especially, obese women ate hotter or saltier food than normal weight women preferring meat. However, no significant differences appeared in marital status, social economic status. occupation. eating habits. smoking. drinking and physical exercise. Social support levels showed a lower rate in obese women than in normal weight women, indicating a statistically significant difference (p<.05). Observing areas of social support, obese women showed lower rates in attachment/intimacy, social integrity, opportunity of foster and confidence in value except help and instruction, which indicated a statistically significant difference (p<.05). Social support for obese women showed significant differences in age, education level, social hierarchy, religion and menstrual status. Obese women were more negative than normal weight women in health recognition, indicating a statistically significant difference (p<.01). Normal weight women showed higher health recognition when provided high social support and significantly low (p<.01) health recognition when provided low social support. However, there was no significant difference in health recognition in obese women whether high or low social support was given. The health recognition of obese women showed significant differences in age, education level, social hierarchy, number of children, menstrual status, physical exercise, eating habits, eating taste and preference of food. Conclusion: Obese women showed elder than normal-weight women, higher body-fat rate and abdomen-fat rate, lower social support, and a tendency to more negative health recognition. Therefore, providing weight-control programs for the treatment of obesity and prevention of recurrence for obese women to prevent progressing to adult disease and promote a healthy life, we suggest that better eating habits and the encouragement of regular physical exercise should be included, as well as total approachment on change of health recognition and social support would be needed.

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Development of a Home-based Nursing Intervention, Mothering Program for Low-Birth-Weight Infants (저체중출생아를 위한 가정간호형 모성역할중재 프로그램 개발과 그 효과에 대한 연구)

  • Han, Kyung-Ja
    • Journal of Korean Academic Society of Home Health Care Nursing
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    • v.8 no.1
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    • pp.5-24
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    • 2001
  • The purpose of this study was to develop a parenting intervention program and determine the efficacy of the program with low-birth weight infants and their mothers. Nine dyads for the experimental group and twelve dyads for the control group discharged from the Neonatal Intensive Care Unit of a University Hospital in Seoul were recruited for the study. For the intervention group, programmed education and support which focused on the maternal sensitivity of the infant's behavior. rearing environment. motherinfant interaction and infant care were given to each subject. Individual counseling and home visits were provided at discharge, one week after discharge. and one and three months of corrected age in every infant. Structured questionaires were administered and feeding interactions were videotaped and coded by a blinded certified observer. A Quasi-experimental design was conducted for this study. Postpartum depression, maternal self esteem. infant care burden, HOME. mother-infant interaction, and infant development were measured. Results were in favor of the intervention versus the control group. On the Beck depression inventory, intervention mothers showed decreasing trends in depressive symptom vs control mothers although, there were statistically no significant differences between the two groups at each time. The mean score of experimental group was 11.55(mild depression state) at discharge and became 8,6(normal state) at 1 month of corrected age. On the other hand, the mean score of the control group was 13.92(mild depression state) at discharge and became 14.0. Maternal self esteem in both groups improved over time. Infant care burden in both groups was also shown to increase over time. There was a significant difference between the two groups in HOME(p=.0340) at 3 months of corrected age. HOME scores of the experimental group and the control's were 31.10 and 25.58, respectively. Mothers' emotional and language responses were significantly high in the intervention group compared with the control group(p=.0155). Intervention group (53.33) showed a significantly high quality of motherinfant interaction compared with the in control group (42.80)(p =.0340). Intervention group mothers appeared have a better quality of mother-infant interaction behaviors. On the other hand, there was no statistical difference in the infant part between groups. Intervention group infants had higher trends in a general developmental quotient: although, there was no statistical difference between groups. The general developmental quotient of intervention infants was 102.56 and control's was 91.28. However, the developmental quotient of the domain of 'individuality-sociality' was higher in the intervention group infants compared with the control's(p=.0155). The concerns identified by parents revealed two domains of an infants' health management -knowledge and skills in caregiving of lowbirthweight-infants, characteristics of lowbirthweight infants, identifying a developmental milestone, coping with emergency situations and relaxation strategies of mothers from the infant care burden. Interview data with the mothers of low-birth weight infants can be used to develop intervention program contents. Limited intervention time and frequency due to time and cost limitations of this study should be modified. The intervention should be continuously implemented when low-birth weight infants become three years old. An NNNS demonstration appeared to be a very effective intervention for the mothers to improve the quality of mother-infant interactions. Therefore intervening in the mothers of low-birth weight infants as early after delivery as possible is desirable. This study has shown that home visit interventions are worthwhile for mothers only beyond the approach as an essential factor in ability of facilitating a growth fostering environment. In conclusion. the intervention program of this study was very effective in enhancing the parenting for the mothers of low-birth weight infants, resulting in health promotion of low-birth weight infants. The home-visit outreach intervention program of this study will contribute to the health delivery system in this country where there is a lack of continuous follow-up programs for low-birth weight infants after discharge from NICU, if it is activated as part of the home visit programs in community health systems.

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The Relationship between Alcohol Drinking Patterns and Blood Pressure in Some Rural Elderly Aged 60~64 (일부 60~64세 농촌노인에서 음주양상과 혈압과의 관련성)

  • Lee, Moo-Sik;Bae, Jang-Ho;Park, Ki-Rack;Lee, Choong-Won
    • Journal of agricultural medicine and community health
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    • v.26 no.1
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    • pp.1-17
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    • 2001
  • This study was conducted to investigate the role of drinking pattern in the relationship of alcohol consumption by face-to-face interview in rural elderly(n=994) aged 60~64 in Dalsung County, April to September in 1996. Pattern of alcohol drinking included drinking status, alcohol drinking at the morning without breakfast, average drinks per day, frequency of drinks per month, quantity of alcohol drinking, kind of preferred alcoholic beverage and duration of alcohol drinking. Blood pressure was measured once in each subject using a portable automatic sphygmomanometer. Difference in means of systolic blood pressure, alcohol drinking status, alcohol drinking at the morning without breakfast, and kind of alcoholic beverage were statistically significant before adjusting covariates, but alcohol drinking status and alcohol drinking at the morning without breakfast were statistically significant after adjusting covariates. And difference in means of diastolic blood pressure, kinds of alcoholic beverage was statistically significant before adjusting covariates, but no variables was significant after adjusting covariates. Model I multiple regression for systolic blood pressure that included average drinks per day as the variable of drink pattern, age, educational attainment and, previous history of cardiovascular disease were statistically significant, and multiple regression for diastolic blood pressure, educational attainment, BMI, and previous history of cardiovascular disease were statistically significant. Model II multiple regression for systolic blood pressure that included drinking patterns variables except average drinks per day, previous history of cardiovascular disease were statistically significant. However, multiple regression for diastolic blood pressure, no variables were significant. So, inconsistent with prior research, a positive relationship was not found between average drinks per day and diastolic and systolic blood pressure. The effect of alcohol drinking patterns on blood pressure has public health as well as clinical relevance. The study should be replicated to determine the reliability of our findings.

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Screw Joint Stability under Cyclic Loading of Zirconia Implant Abutments (지르코늄 임플란트 지대주의 나사결합부 안정성에 관한 연구)

  • Lee, Mi-Soon;Suh, Kyu-Won;Ryu, Jae-Jun
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.2
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    • pp.164-173
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    • 2009
  • Purpose: The purpose of this study was to evaluate the effect of abutment material on screw-loosening before and after cyclic loading. Among the different materials of abutments, zirconia and metal abutment were used. Material and methods: Two types of implant systems: external butt joint(US II, Osstem Implant, Korea) and internal conical joint(GS II, Osstem Implant, Korea) were used. In each type, specimens were divided into two different kinds of abutments: zirconia and metal(n=5). The implant was rigidly held in a special holding to device ensure fixation. Abutment was connected to 30 Ncm with digital torque gauge, and was retightened in 30 Ncm after 10 minutes. The initial removal torque values were measured. The same specimens were tightened in 30 Ncm again and held in the cycling loading simulator(Instron, USA) according to ISO/FPIS 1480. Cycling loading tests were performed at loads 10 to 250 N, for 1 million cycles, at 14 Hz,(by subjecting sinusoidal wave from 10 to 250 N at a frequency of 14 Hz for 1 million cycles,) and then postload removal torque values were evaluated. Results: 1. In all samples, the removal values of abutment screw were lower than tightening torque values(30 Ncm), but the phenomenon of the screw loosening was not observed. 2. In both of the implant systems, initial and postload removal torque of zirconia abutment were significantly higher than those of metal abutment(P<.05). 3. In both of the implant systems, the difference in removal torque ratio between zirconia abutment and metal abutment was not significant(P>.05). 4. In metal abutments, the removal torque ratio of GS II system(internal conical joint system) was lower than that of US II system(external butt joint system)(P<.05). 5. In zirconia abutments, the difference in removal torque ratio between the two implant systems was not significant(P>.05). Conclusion: Zirconia abutment had a good screw joint stability in the condition of one million cycling loading.

Tensile bond strength of chairside reline resin to denture bases fabricated by subtractive and additive manufacturing (적층가공과 절삭가공으로 제작한 의치상과 직접 첨상용 레진 간의 인장결합강도 비교)

  • Kim, Hyo-Seong;Jung, Ji-Hye;Bae, Ji-Myung;Kim, Jeong-Mi;Kim, Yu-Lee
    • The Journal of Korean Academy of Prosthodontics
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    • v.58 no.3
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    • pp.177-184
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    • 2020
  • Purpose: The purpose of this study was to compare and evaluate the tensile bond strength of chairside reline resin to denture base resin fabricated by different methods (subtractive manufacturing, additive manufacturing, and conventional heat-curing). Materials and methods: Denture base specimens were fabricated as cuboid specimens with a width of 25 mm × length 25 mm × height 3 mm by subtractive manufacturing (VITA VIONIC BASE), additive manufacturing (NextDent Base) and conventional heat-curing (Lucitone 199). After storing the specimens in distilled water at 37℃ for 30 days and drying them, they were relined with polyethyl methacrylate (PEMA) chairside reline resin (REBASE II Normal). The subtractive and additive manufacturing groups were set as the experimental group, and the heat-curing group was set as the control group. Ten specimens were prepared for each group. After storing all bound specimens in distilled water at 37℃ for 24 hours, the tensile bond strength between denture bases and chairside reline resin was measured by a universal testing machine at a crosshead speed of 10 mm/min. The fracture pattern of each specimen was analyzed and classified into adhesive failure, cohesive failure, and mixed failure. Tensile bond strength, according to the fabrication method, was analyzed by 1-way ANOVA and Bonferroni's method (α=.05). Results: Mean tensile bond strength of the heat-curing group (2.45 ± 0.39 MPa) and subtractive manufacturing group (2.33 ± 0.39 MPa) had no significant difference (P>.999). The additive manufacturing group showed significantly lower tensile bond strength (1.23 ± 0.36 MPa) compared to the other groups (P<.001). Most specimens of heat-curing and subtractive manufacturing groups had mixed failure, but mixed failure and adhesive failure showed the same frequency in additive manufacturing group. Conclusion: The mean tensile bond strength of the subtractive manufacturing group was not significantly different from the heat-curing group. The additive manufacturing group showed significantly lower mean tensile bond strength than the other two groups.

Diagnosis and Effect of Maxillary Expansion in Pediatric Sleep-Disordered Breathing (소아 수면호흡장애의 진단과 상악확장술의 치료효과)

  • Kim, Doyoung;Baek, Kyounghee;Lee, Daewoo;Kim, Jaegon;Yang, Yeonmi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.46 no.4
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    • pp.369-381
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    • 2019
  • The aim of this study was to analyze the changes and improvements in symptoms of sleep-disordered breathing (SDB) using semi-rapid maxillary expansion (SRME) in children with narrow maxilla and SDB symptoms. Subjects were 15 patients with sleep disorder (apnea-hypopnea index, AHI ≥ 1) and narrow maxillary arch between 7 and 9 years of age. Before the SRME was applied, all subjects underwent pediatric sleep questionnaires (PSQ), lateral cephalometry, and portable sleep monitoring before expansion (T0). All subjects were treated with SRME for 2 months, followed by maintenance for the next 3 months. All subjects had undergone PSQ, lateral cephalometry, and portable sleep monitoring after expansion (T1). Adenoidal-nasopharyngeal ratio (ANR), upper airway width and hyoid bone position were measured by lateral cephalometry. The data before and after SRME were statistically analyzed with frequency analysis and Wilcoxon signed rank test. As reported by PSQ, the total PSQ scale was declined significantly from 0.45 (T0) to 0.18 (T1) (p = 0.001). Particularly, snoring, breathing, and inattention hyperactivity were significantly improved (p = 0.001). ANR significantly decreased from 0.63 (T0) to 0.51 (T1) (p = 0.003). After maxillary expansion, only palatopharyngeal airway width was significantly increased (p = 0.035). There was no statistically significant difference in position of hyoid bone after expansion (p = 0.333). From analysis of portable sleep monitoring, changes in sleep characteristics showed a statistically significant decrease in AHI and ODI, and the lowest oxygen desaturation was significantly increased after SRME (p = 0.001, 0.004, 0.023). In conclusion, early diagnosis with questionnaires and portable sleep monitoring is important. Treatment using SRME will improve breathing of children with SDB.

Multiplication of Escherichia coli DH5α::gfp on Strawberry Fruit Surface (딸기과실 표면에서 Eschercia coli DH5α::gfp 증식)

  • Yun, Hyejeong;Park, Kyeonghun;Ryu, Kyoung Yul;Yun, Jong-Chul;Kim, Byung Seok
    • Food Science and Preservation
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    • v.20 no.2
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    • pp.250-256
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    • 2013
  • To verify the multiplication of microorganisms on the surface of strawberries, the fate of E. coli $DH5{\alpha}::gfp$ at different temperatures, times and strawberry extract concentrations were measured. The population of E. coli $DH5{\alpha}::gfp$ rapidly increased by 7.36~7.78 log CFU/g at $25{\sim}30^{\circ}C$ for 24 hr and slowly increased by 6.49~8.49 log CFU/g at $10{\sim}20^{\circ}C$ for 48 hr. However, E. coli $DH5{\alpha}::gfp$ did not grow at $10{\sim}15^{\circ}C$ on the surface of the strawberries, regardless of the contact times with the bacterial suspension. E. coli $DH5{\alpha}::gfp$ reached 1.52~3.26 log CFU/g at $20^{\circ}C$ as the contact frequency increased from two to six times. The contact frequencies did not significantly differ. In the case of the six-time contact on the surface of the strawberry at 25 and $30^{\circ}C$, the E. coli $DH5{\alpha}::gfp$ increased by 5.17 and 5.01 log CFU/g. The effects of the strawberry extracts on the growth of E. coli $DH5{\alpha}::gfp$ showed that sterilization and non-sterilization do not affect the growth of microorganisms for 96 hr. In the minimal broth, the growth of E. coli $DH5{\alpha}::gfp$ increased by 1 log CFU/g for 96 hr. In less than 50 percent of the strawberry extracts, the growth rate of E. coli $DH5{\alpha}::gfp$ was higher than in the control and increased by 4 and 5 log CFU/g at 50 and 25 percent of strawberry extracts, respectively. Therefore, E. coli $DH5{\alpha}::gfp$ can multiply and survive on the surface of strawberries when it comes into contact with the fruit extract.