• JSTS:Journal of Semiconductor Technology and Science
• /
• 제11권4호
• /
• pp.229-237
• /
• 2011

In this paper, a 60 GHz LTCC SiP with low-power CMOS OOK modulator and demodulator is presented. The 60 GHz modulator is designed in a 90-nm CMOS process. The modulator uses a current reuse technique and only consumes 14.4-mW of DC power in the on-state. The measured data rate is up to 2 Gb/s. The 60 GHz OOK demodulator is designed in a 130nm CMOS process. The demodulator consists of a gain boosting detector and a baseband amplifier, and it recovers up to 5 Gb/s while consuming low DC power of 14.7 mW. The fabricated 60 GHz modulator and demodulator are fully integrated in an LTCC SiP with 1 by 2 patch antenna. With the LTCC SiP, 648 Mb/s wireless video transmission was successfully demonstrated at wireless distance of 20-cm.

• Journal of Microbiology and Biotechnology
• /
• 제33권11호
• /
• pp.1437-1447
• /
• 2023

A recently bioinformatic analysis of genomic sequences of fungi indicated that fungi are able to produce more secondary metabolites than expected. Despite their potency, many biosynthetic pathways are silent in the absence of specific culture conditions or chemical cues. To access cryptic metabolism, 108 fungal strains isolated from various sites were cultured with or without Streptomyces sp. 13F051 which mainly produces trichostatin analogues, followed by comparison of metabolic profiles using LC-MS. Among the 108 fungal strains, 14 produced secondary metabolites that were not recognized or were scarcely produced in mono-cultivation. Of these two fungal strains, Myrmecridium schulzeri 15F098 and Scleroconidioma sphagnicola 15S058 produced four new compounds (1-4) along with a known compound (5), demonstrating that all four compounds were produced by physical interaction with Streptomyces sp. 13F051. Bioactivity evaluation indicated that compounds 3-5 impede migration of MDA-MB-231 breast cancer cells.

• 한국식품저장유통학회지
• /
• 제19권3호
• /
• pp.420-427
• /
• 2012

본 연구는 옻나무 추출물(Rhus verniciflua Stokes extract, RVSE)의 침지가 가스조절포장(modified-atmosphere packaging, MAP)으로 저장한 한우고기 스테이크의 총환원력, 단백질산화 및 육색소산화에 미치는 영향을 구명하고자 실시하였다. 시료(Hanwoo longissimus muscle)는 옻나무 추출물의 침지 여부에 따라 처리한 후 high oxygen-MAP(HOMAP; 75% $O_2$+20% $CO_2$+5% $N_2$)와 low oxygen-MAP (LOMAP; 0% $O_2$+20% $CO_2$+80% $N_2$)으로 포장하여 $4^{\circ}C$에서 9일 동안 저장하였다. 총환원력은 RVSE의 침지에 의해 증가되었으며(p<0.05), LOMAP에 의해 저장기간 동안 높게 유지되었다. Carbonyl 함량은 RVSE의 침지와 LOMAP에 의해 억제되었다. Metmyoglobin의 축적은 RVSE의 침지에 의해 증가되었으나, HOMAP에 의해 낮게 유지되었다. 이와 반대로 oxymyoglobin에 의한 적색 강도의 지표인 R630-R580은 RVSE의 침지에 의해 감소되었던 반면, HOMAP에 의해 높게 유지되었다. 명도($L^*$), 적색도($a^*$) 및 chroma($C^*$)는 RVSE의 침지에 의해 감소되었으나, HOMAP에 의해 높게 유지되었다. 반면에 hue-angle($H^{\circ}$)은 RVSE의 침지에 의해 증가되었으나, HOMAP에 의해 낮게 유지되었다. 따라서 RVSE의 침지는 한우고기 스테이크의 총환원력과 단백질산화 안정성을 증가시켰으나, 육색소산화 안정성 감소시켰다. 또한 HOMAP는 총환원력과 단백질산화 안정성을 감소시켰으나, 육색소산화 안정성은 증가시켰다.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2015년도 춘계학술대회 및 임시총회
• /
• pp.41-41
• /
• 2015

BSA-seq technologies, combined Bulked Segregant Analysis (BSA) and Next-Generation Sequencing (NGS), are making it faster and more efficient to establish the association of agronomic traits with molecular markers or candidate genes, which is the requirement for marker-assisted selection in molecular breeding. Clubroot disease, caused by Plasmodiophora brassicae, is a serious threat to Brassica crops. Even we have breed new clubroot resistant varieties of Chinese cabbage (B. rapa ssp. pekinesis), the underlying genetic mechanism is unclear. In this study, an $F_2$ population of 340 plants were inoculated with P. brassicae from Xinye (Pathotype 2 on the differentials of Williams). Resistance phenotype segregation ratio for the populations fit a 3:1 (R:S) segregation model, consistent with a single dominant gene model. Super-BSA, using re-sequencing the parents, extremely R and S DNA pools with each 50 plants, revealed 3 potential candidate regions on the chromosome A03, with the most significant region falling between 24.30 Mb and 24.75 Mb. A linkage map with 31 markers in this region was constructed with several closely linked markers identified. A Major QTL for clubroot resistance, CRq, which was identified with the peak LOD score at 169.3, explaining 89.9% of the phenotypic variation. And we developed a new co-segregated InDel marker BrQ-2. Joint BSA-seq and traditional QTL analysis delimited CRq to an 250 kb genomic region, where four TIR-NBS-LRR genes (Bra019409, Bra019410, Bra019412 and Bra019413) clustered. The CR gene CRq and closely linked markers will be highly useful for breeding new resistant Chinese cabbage cultivars.

• 한국환경성돌연변이발암원학회지
• /
• 제26권1호
• /
• pp.12-19
• /
• 2006

Cadmium, a human carcinogen, can induce toxicity in various cell lines and organs. Despite extensive research, the mechanisms of cadmium-induced cell toxicity and estrogenic potential in human are not clear. This study was performed to investigate cadmium-induced toxicity on human breast cancer cells: MCF-7 cells, an estrogen receptor (ER) positive breast cancer cells, and MDA-MB-231 cells, an ER negative breast cancer cells. MCF-7 cells was proved to be more sensitive than the other cell lines (IC50 = $50\;{\mu}M$ at MCF-7 cells and $120{\mu}M$ at MDA-MB-231). The expression of JNK and AP-1 transcription factors such as c-Jun and c-Fos dependent transcription were increased by cadmium treatment. Inhibition of ER activation by ER antagonist (tamoxifen or ICI 182,780) significantly recovered the viablity and inhibited apoptotic cell death. This suggested that cadmium-induced cell death in ER (+) cells was mediated by JNK/AP-1 pathway and this pathway was more stimulated by ER activated by cadmium. Co-treatment of antioxidants such as selenium (Se), butylated hydroxyanisole (BHA), glutathione (GSH), or N-acetyl-L-cysteine (NAC) recovered the cadmium-induced cell death in MCF-7 cells. Cadmium-induced lipid peroxidation was decreased by GSH, NAC, or BHA in MCF-7 cells. The expression of SOD protein was decreased by cadmium ($100{\mu}M$) but recovered by GSH, NAC, BHA, or Se. Our data showed that the cadmium-induced cell toxicity in human breast cancer cells could be protected by the antioxidants (Se, BHA, NAC, GSH, or NAC) and ER antagonist (tamoxifen or ICI 182,780). Therefore, toxicity of cadmium in breast cancer were mediated by oxidative stress and $ER{\alpha}$.

• 대한임상독성학회지
• /
• 제3권1호
• /
• pp.27-32
• /
• 2005

Purpose: The frequency of carbon monoxide poisoning has been decreased in the interior of the Korea. But occasionally it is occurred and the risk of exposure is high in working place so far. Because of the characteristics of gas, the detection of exposure and poisoning could be delayed and fatality is high. We should apprehend of carbon monoxide poisoning. So we would report analysis of patients with carbon monoxide poisoning. Methods: A retrospective review of CO poisoned patients visited emergency department from January 2000 to December 2004 was conducted. Results: 24 patients were enrolled. Their average of age was $37.6\pm20.9$ years old and COHb was $19.4\pm13.32\%$. The blood level of initial COHb and mental status on arrival were not correlated each other. The blood level of initial COHb and loss of consciousness were not correlated, too. Initial electrocardiography (EKG) was not correlated with cardiac enzymes such as CK-MB and troponin I. But base excess was correlated with mental status on arrival and complication such as rhabdomyolysis. Hyperbaric oxygen therapy was correlated with base excess and mental status on arrival. Conclusion: The clinical features of carbon monoxide poisoning are nonspecific. For proper diagnosis, it is important that we should consider patient's environment and take patient's history carefully. The blood level of initial COHb does not reflect severity of poisoning accurately. So We should determine the treatment of choice depending on patient's status.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권8호
• /
• pp.1078-1087
• /
• 2018

Objective: The genetic diversity of the Landrace population, a representative maternal pig breed in Korea, is important for genetic improvement. Previously, the effective population size (Ne) has been used to infer the genetic diversity of a population of interest. In this study, we aimed to use single nucleotide polymorphism (SNP) data to characterize linkage disequilibrium (LD) and the Ne of the Korean Landrace population. Methods: We genotyped 1,128 Landrace individuals from three representative Korean major grand-grand-parent (GGP) farms using the Illumina PorcineSNP60 version2 BeadChip, which covers >61,565 SNPs located across all autosomes and mitochondrial and sex chromosomes. We estimated the expected LD and current Ne, as well as ancestral Ne. Results: In the Korean Landrace population, the mean LD ($r^2$) of 3.698 million SNP pairs was $0.135{\pm}0.204$. The mean $r^2$ decreased slowly with as the distance between SNPs increased, and remained constant beyond 3 Mb. According to the $r^2$ calculations, 8,085 of 3.698 million SNP pairs were in complete LD. The current Ne (${\pm}$standard deviation) of the Korean Landrace population is approximately 92.27 [79.46; 105.07] individuals. The ancestral Ne exhibited a slow and steady decline from 186.61 to 92.27 over the past 100 generations. Additionally, we observed more a rapid Ne decrease from the past 20 to 10 generations ago, compared with other intervals. Conclusion: We have presented an overview of LD and the current and ancestral Ne values in the Korean Landrace population. The mean LD and current Ne for the Korean Landrace population confirm the genetic diversity and reflect the history of this pig population in Korea.

• Bulletin of the Korean Chemical Society
• /
• 제30권7호
• /
• pp.1463-1469
• /
• 2009

Eleven novel oxiranyl and thiiranyl phenolic compounds were synthesized as potential antitumor agents using epichlorohydrin and epithiohydrin in the presence of $K_2CO_3$. Cytotoxicities were found in range of I$C_{50}$ values of 2.5-14.8 $\mu$M, which was partially attributed to topoisomerase II inhibition. Bis-thiiranyl anthraquinone analog, 19 showed more cytotoxicity against MDA-MB-231 (breast cancer cell) and PC3 (prostate cancer cell) after 24 and/or 48 h and more potent topoisomerase II inhibitory activity than etoposide.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제11권2호
• /
• pp.121-129
• /
• 2011

It is progressing as new advents and remarkable developments of mobile device every year. On the upper line reason, NAND FLASH large density memory demands which can be stored into portable devices have been dramatically increasing. Therefore, the cell size of the NAND Flash memory has been scaled down by merely 50% and has been doubling density each per year. [1] However, side effects have arisen the cell distribution and reliability characteristics related to coupling interference, channel disturbance, floating gate electron retention, write-erase cycling owing to shrinking around 20nm technology. Also, FLASH controller to manage shrink effect leads to speed and current issues. In this paper, It will be introduced to solve cycling, retention and fail bit problems of sub-deep micron shrink such as Virtual negative read used in moving read, randomization. The characteristics of retention, cycling and program performance have 3 K per 1 year and 12.7 MB/s respectively. And device size is 179.32 $mm^2$ (16.79 mm ${\times}$ 10.68 mm) in 3 metal 26 nm CMOS.

• Applied Biological Chemistry
• /
• 제51권2호
• /
• pp.142-147
• /
• 2008

Rhizopus oryzae KSD-815를 밀에 접종하여 만든 누룩(Rhizopus oryzae KSD-815)에서 4종의 지질 화합물을 분리하여 화학구조를 동종하였다. 건조된 누룩을 실온에서 80% MeOH 수용액으로 추출하고 이추출물을 EtOAc 분획, n-BuOH 분획, $H_2O$ 분획으로 나누었다. EtOAc분획은 다시 80% MeOH 과 n-hexane 으로 분획하였다. n-Hexane 분획에 대해 silica gel 및 ODS column chromatography를 반복 실시하여 4종의 지질 화합물을 분리, 정제하였다. NMR, IR, GC/MS 등을 통하여 화합물 1(linolenic acid methyl ester), 화합물 2(palmitic acid methyl ester), 화합물 3(linoleic acid), 화합물 4(palmitic acid)의 구조를 결정하였다. 이 지방산 화합물의 세포독성을 평가하기 위해 인체 유래 유방암(MDA-MB-231)과 간암(SK-HEP-1)세포주에 대해 MTT assay를 수행하였다. 두 암 세포주에서 화합물 1(linolenic acid methyl ester)과 화합물 3(linoleic acid)은 농도의존적인 세포독성을 확인하였다. 또 염증반응 매개체의 일종인 nitric oxice(NO)의 생성 억제 활성을 Griess 방법으로 평가한 결과, 화합물 3(linoleic acid)은 LPS와 IFN-${\gamma}$에 의해 유도 된 NO 생성도 저해하는 것을 RAW264.7 세포에서 확인하였다.