• Journal of Ginseng Research
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• 제11권2호
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• pp.111-117
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• 1987

저년생 인삼의 잠아 및 화기형성시기와 양상은 고년생 인삼의 이차적인 양상과 상이하기 때문에 1년생 묘삼의 잠아발달 및 다식된 2년생 인삼의 화기형성과정을 조사관찰하였던 바 그 결과를 요약하면 다음과 같다. 1. 2년생 이상의 인삼 잠아조직은 4월중순경 분제조직의 조기포분제로 시작하는데 1년생 묘삼에서는 유근발근시인 3월 하순경 시작하는 것으로 추측되었으며 잠아의 위치는 자엽간 줄기하단 문부위에 생성되었다. 2. 3년생 이상의 인삼 화기분화는 전년도 형성된 뇌두조직내에서 형성되지만 2년생 인삼의 화기는 줄기선단 엽병사이의 경단분제조직에서 분제세포의 분열과 함께 분화, 형성되었다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.213-237
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• 1987

In vitro flowering system may minimize the confounded influence of non-floral meristem parts of plants in studying the relationship of a given treatment and flowering responses. We have induced flower buds from plantlets regenerated from zygotic embryo-derived somatic embryos of ginseng, which circumvented the normal 2-year juvenile period before flowering. The result suggests that the adulthood of ginseng root explants in the experiment previously conducted by Chang and Hsing (1980; Nature 284: 341-342) is not prerequired to flowering of plantlets regenerated through somatic embryogenesis. We have also induced flower buds from elongated axillary brandches from cotyledonary nodes by culturing ginseng zygotic embryos, seedlings, and excised cotyledonary nodes. It was found that 6-benzyladenine (BA) supplemented to the medium was essential for flowering, whereas abscisic acid (ABA) was inhibitory. Gibberellic acid(GA3) was also required for flowering when ABA was present with BA in the medium. The results suggest that cytokinins, gibberellins, and inhibitors play primary, permissive, and preventive roles, respective-ly, in the induction of flowering of ginseng. Tran Thanh Van (1980; Int. Rev. Cytol., Suppl. IIA: 175-194) has developed the "thin cell layer system" in which the induction of shoots, roots, or flower buds from epidermal layer explants were controlled by culture conditions and exogenous growth regulators in the medium, Utilizing the thin cell layer system, Meeks-Wagner et al. (1989; The Plant Cell 1: 25-35) have cloned genes specifically expressed during floral evocation. However, the system is too tedious for obtaining a sufficient amount of plant materials for biochmical and molecular biological studies of flowering. We have developed a garlic callus culture system and one obvious advantaging over the thin cell layer system is that an abundant cells committed to develope into flower buds proliferate. When the above cells were compared by two-dimensional gel electrophoresis with those which have just lost the competence for developing into flower buds, a few putative proteins specific to floral evocation were detected. The garlic callus culture system can be further explored for elucidation of the molecular biological mechanism of floral evocation and morphogenesis.hogenesis.

• 농업생명과학연구
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• 제45권1호
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• pp.49-58
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• 2011

본 연구는 밤나무 품종개량을 위한 기초자료를 제공하기 위하여 남부지방에서 재배되고 있는 31품종의 개엽 및 개화특성을 조사하였다. 개엽시기는 품종 간에 약간의 차이가 있었으며, 2008년과 2009년에 비해 2010년에 늦게 개엽되었다. 개엽 1단계부터 4단계까지 소요되는 기간은 2008년과 2009년은 약 8일 정도였지만, 2010년에는 약 14일 정도가 소요되었다. 개화특성에 있어서 2008년과 2009년의 경우, 암꽃의 개화개시와 개화완료는 각각 5월 초순과 6월 초순이었으나 2010년에는 늦은 개화개시와 개화완료를 나타내었다. 개화특성에 있어서는 수꽃도 암꽃과 동일한 경향이었으나, 개화개시는 수꽃이 암꽃보다 20일 정도 빠르게 나타났다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.77-77
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• 2017

The sensitivities to photoperiod and temperature give guidance to choose an adaptable genotype for specific area in soybean production. However, there is insufficient information about the variation of sensitivities to photoperiod and temperature with wide genetic background. We investigated the sensitivities to photoperiod and temperature using 82 soybean mini core collection lines provided by NIAS gene bank of Japan. The seeds were sown on 28 May and 4 August in 2015, 24 May and 5 August in 2016 at field in Saga, Japan ($33^{\circ}$ 14' 32'' N, $130^{\circ}$ 17' 28'' E) for the early (average photoperiod and temperature: 15.2 h and $25.1^{\circ}C$) and late (13.6 h and $27.2^{\circ}C$) sowing respectively. The plants were also grown in the growth chamber under 12 h photoperiod with three temperature regimes (day/night temperature: $25/18^{\circ}C$, $28/22^{\circ}C$ and $33/28^{\circ}C$). Emergence date, days to first flower were recorded with 10 plants in the field and 2 plants in the growth chamber for each line. The data for daily average temperatures and photoperiodic hours were collected from weather station. The days from emergence to first flower open (DEF) were varied from 23-92 (2015 and 2016) in early sowing whereas 18-68 (2015) and 18-59 (2016) in late sowing. The shortened DEF in late sowing could be caused by both short photoperiod and high temperature in late sowing. However, the accumulated temperatures during emergence to first flower open (ATEF) were less variable in comparison with DEF, suggesting the ATEF is dependent mostly on the photoperiod. The ATEF were found same between early and late sowing in some early flowering lines (e.g. $686.7^{\circ}C$ and $687.6^{\circ}C$ in HEUKDAELIPS, $728.8^{\circ}C$ and $706.3^{\circ}C$ in WILLIAMS'82) which indicated that these would be insensitive to day length. In the growth chamber experiment, the variation in both DEF and ATEF was a little greater at low temperature ($25/18^{\circ}C$) but almost same at middle ($28/22^{\circ}C$) and high ($33/28^{\circ}C$) temperatures. Since the less differences in ATEF were found between the three temperatures, it is suggested that the temperature plays only a quantitative effect on the flower initiation, and the large ATEF in some lines may indicate the stronger photosensitivity even at 12 h or longer juvenile phase. Some lines with the lowest ATEF regardless of growth conditions, such as FISKEBY V, KE 32 (ATEF: 559.6-666.5, 587.7-709.5) might lack the sensitivities to both photoperiod and temperature. The results suggested that soybean genotypes has wider variation in sensitivity to photoperiod, whereas less variation to temperature.

• 한국식품저장유통학회지
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• 제13권6호
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• pp.691-696
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• 2006

감 품종별(도근조생, 고종시, 갑주백목, 차랑, 월하시, 상주둥시)로 감꽃을 개화기에 채취하여 동결건조 분말화한 감꽃분말에 함유된 유효성분을 분석, 비교하였다. 조지방 함량은 도근조생이 57.26%로 가장 높은 값을 보였으며, 유리당 조성은 fructose가 $95{\sim}310 mg%$, glucose는 $75{\sim}281mg%$, sucrose가 $7{\sim}28mg%$의 함유량을 나타내었고, 유기산 중 malic acid가 갑주백목(6월4일)에 225 mg%로 가장 많았고, tartaric acid도 189 mg%로 가장 많았다. 유리아미노산 중 hydroxy-L-proline은 월하시에 25.33 mg%, L-citrulline 은 상주둥시(5월28일)가 58.83 mg%, L-threonine은 상주둥시(5월28일)가 11.88 mg%로 가장 많았다. 페놀산으로 caffeic acid는 갑주백목(6월4일)이 $1,946{\mu}g/100g$, p-hydioxybenzoic acid는 상주둥시(5월29일)가 $417.8{\mu}g/100 g$, protocatechuic acid는 갑주백목(6월1일)이 $181.1{\mu}g/100g$으로 가장 많았다. 따라서 감꽃에도 감이나 감잎에 못지 않게 유용한 식품성분들과 기능성 성분들이 다량 함유되어 있어 새로운 식품 및 기능성 신소재로서의 가치가 매우 높다고 할 수 있다.

• Korean Chemical Engineering Research
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• 제54권1호
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• pp.75-80
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• 2016

Biologically stabilized gold nanoparticles were synthesized from the flower aqueous extract of T. divaricata. The synthesized nanoparticles were characterized by UV-Vis spectrophotometer, Zeta sizer, FTIR and TEM analysis. T. divaricata reduced gold nanoparticles having particle size and potential of 106.532 nm and -10.2 mV, respectively, with a characteristic peak of 550 nm in UV-visible spectrophotometer. FTIR graph after comparison between the crude flower extract and gold nanoparticles showed three major shifts in the functional groups. The morphology and size of the gold nanoparticles were examined by HRTEM analysis, which showed that most of the nanoparticles were nearly spherical with size of 100 nm. The gold nanoparticles synthesized demonstrated potent anticancer activity against MCF-7 cell line. The findings conclude that the antioxidant molecule present in T. divaricata may be responsible for both reduction and capping of gold nanoparticles which possess potential applications in medicine and pharmaceutical fields.

• Parasites, Hosts and Diseases
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• 제54권3호
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• pp.273-280
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• 2016

We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays. In particular, microfilarial release, worm motility, and viability were determined. M. cajuputi flower extracts were found to significantly reduce Wolbachia endosymbionts in Aa23 cells, Wolbachia surface protein, and microfilarial release, as well as the viability and motility of adult worms. Anti-Wolbachia activity was further confirmed by observation of degraded and phagocytized Wolbachia in worms treated with the flower extracts. The data provided in vitro and in vivo evidence that M. cajuputi flower extracts inhibit Wolbachia, an activity that may be exploited as an alternative strategy to treat human lymphatic filariasis.

• The Plant Pathology Journal
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• 제26권1호
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• pp.57-62
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• 2010

Low-molecular-weight secondary metabolites from plants play an important role in reproductive processes and in the defense against environmental stresses or pathogens. In the present study, we isolated various volatiles and phenolic compounds from white Rosa rugosa flowers, and evaluated the pharmaceutical activities of these natural products in addition to their ability to increase survival in response to environmental stress and pathogen invasion. The DPPH and hydroxyl radical-mediated oxidation assay revealed that the white rose flower extract (WRFE) strongly scavenged free radicals in a dose dependent manner. Moreover, WRFE inhibited the growth of E. coli and fatally attacked those cells at higher concentration (>0.5 mg/mL). FITC-conjugated Annexin V stain provided further evidence that WRFE had strong antimicrobial activity, which may have resulted from a cooperative synergism between volatiles (e.g. 1-butanol, dodecyl acrylate and cyclododecane) and phenolic compounds (e.g. gallic acid) retained in WRFE. In conclusion, secondary metabolites from white rose flower hold promise as a potential natural source for antimicrobial and non-chemical based antioxidant agents.

• Journal of Ginseng Research
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• 제29권2호
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• pp.71-79
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• 2005

In vitro studies using detached inflorescences with peduncles were conducted to investigate flower abscission agents in North American ginseng (Panax quinquefolius L.). Of the nine compounds studied only three, ammonium thiosulphate (ATS), abscisic acid (ABA) and ethephon induced abscission. Anilazine, benzyladenine, carbaryl, gibberellic acid, napthaleneacetic acid and thidiazuron did not induce abscission. ATS dip treatments did not induce abscission but the spray treatments induced $60.5\%$ abscission at $1500\;mg{\cdot}L^{-1}$ and $33.1\%$ at $3000\;mg{\cdot}L^{-1}$. Severe chlorophyll loss occurred on all inflorescences treated with ATS. Both ABA dip treatments and a $250\;{\mu}mol{\cdot}L^{-1}$ spray treatment caused abscission $(40\%)$ without adverse effects, and timing of ABA application was important. Because ABA was only significant in the dip treatments, ABA may not be a practical option for field use on ginseng. Ethephon sprays induced more abscission as the season progressed and as the concentration increased. As the dip concentrations of ethephon increased, the abscission rate decreased and the health of the inflorescences declined. The $1500\;mg{\cdot}L^{-1}$ spray of ethephon gave consistent abscission results over the glowing season with little phytotoxicity. Treatment with the competitive ethylene inhibitor 1-methylcy-clopropene (1-MCP) suggested that flower abscission was due to the liberation of ethylene from the breakdown of ethephon.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.69-77
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• 2003

The ornamental industry encompasses cut flower, pot plant, turfgrass and nursery stock production and is an important part of the agricultural sector. As internationally traded commodities, cut flowers and plants are an integral part of the economy of a number of developing countries in South America, the Caribbean and Africa. Genetic modification (GM) is a tool with great potential to the ornamental horticulture industry. The rapid progress in our knowledge of plant molecular biology can accelerate the breeding ornamental plants using recombinant DNA technology techniques. Not only is there the possibility of creating new, novel products the driver of the industry but also the potential to develop varieties requiring less chemical and energy inputs. As an important non-food agricultural sector the use of genetically modified (GM) ornamental crops may also be ideal for the intensive farming necessary to generate pharmaceuticals and other useful products in GM plants. To date, there are only a few ornamental GM products in development and only one, a carnation genetically modified for flower colour, in the marketplace. International Flower Developments, a joint venture between Florigene Ltd. in Australia and Suntory Ltd. of Japan, developed the GM carnations. These flowers are currently on sale in USA, Japan and Australia. The research, development and commercialization of these products are summarized. The long term prospects for ornamental GM products, like food crops, will be determined by the regulatory environment, and the acceptance of GM products in the marketplace. These critical factors will be analysed in the context of the current legislative environment, and likely public and industry opinion towards ornamental genetically modified organisms (GMO's).