• Korean Journal of Environmental Biology
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• v.40 no.3
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• pp.247-254
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• 2022

To investigate the tolerance limit and critical thermal maximum (CTM), behavioral responses of wild goldeye rockfish Sebastes thompsoni according to exposure to high water temperature were observed using a continuous behavior tracking system. As a result, behavioral index (BI) of S. thompsoni in each temperature (20.0, 25.0, and 30.0℃) showed a significant difference (p<0.05) when compared with the value measured in a stable condition of 15.0℃. The activity level of S. thompsoni exposed to 25.0℃ decreased sharply after 20 hours. Their rest time at the bottom of experiment chamber increased, and their normal swimming and metabolic activities were disturbed. In addition, at a high water temperature of 30.0℃, S. thompsoni reached the limit of resistance and showed a sub-lethal reaction of swimming behavior, with energy consumption in the body increased and all test organisms died. In conclusion, the eco-physiological response of S. thompsoni to water temperature varied greatly depending on the fluctuation range of the exposed temperature and the exposure time. In addition, the tolerance limit of S. thompsoni to high water temperature was predicted to be 25.0-30.0℃. The maximum critical thermal that had a great influence on the survival of this species was found to be around 30.0℃.

• ALGAE
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• v.35 no.4
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• pp.375-388
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• 2020

Ocean acidification and warming, identified as environmental concerns likely to be affected by climate change, are crucial determinants of algal growth. The ichthyotoxic raphidophytes Chattonella species are responsible for huge economic losses and environmental impact worldwide. In this study, we investigated the impact of CO2 on the thermal performance curves (TPCs) of Chattonella marina and Chattonella ovata grown under temperatures ranging from 13 to 34℃ under ambient pCO2 (350 μatm) and elevated pCO2 (950 μatm). TPCs were comparable between the species or even between pCO2 levels. With the exception of the critical thermal minimum (CTmin) for C. ovata, CTmin for C. marina and the thermal optimum (Topt) and critical thermal maximum (CTmax) for both species did not change with elevation of pCO2 levels. While CO2 enrichment increased the maximum photosynthetic rates (Pmax) up to 125% at the Totp of 30℃, specific growth rates were not significantly different under elevated pCO2 for the two species. Overall, C. ovata is likely to benefit from climate change, potentially widening its range of thermal tolerance limit in highly acidic waters and contributing to prolonged phenology of future phytoplankton assemblages in coastal waters.

• Journal of Korean Society on Water Environment
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• v.27 no.3
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• pp.314-321
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• 2011

The potential effects of increased water temperature on fish assemblages and their habitats were studied in the streams of the Han River watershed when the water temperature was supposed to increase by $1^{\circ}C$, $2^{\circ}C$, and $3^{\circ}C$ in each sampling site. The percent changes in suitable habitats for each species and in species number within a site were determined, based on the estimated maximum thermal tolerances of 51 fish species whose habitats were classified into four reaches: upstream, up-/midstream, midstream, and mid-/downstream. The maximum thermal tolerance ranged between $25.0^{\circ}C$ and $31.0^{\circ}C$ and significantly increased as the habitat reaches move from upstream to mid-/downstream. With the increases in water temperature, the average suitable habitats for all 51 species were decreased by 31% ($+1^{\circ}C$), 46% ($+2^{\circ}C$), and 60% ($+3^{\circ}C$). The increased water temperature, however, did not induce significant differences in the changes in suitable habitats among four reaches within each level of temperature increase. The relative frequencies of the sampling sites classified into six levels according to the percent change in the number of species in each site were significantly different among three levels of water temperature increase, with relatively greater changes in the sites where 0~20% and 61~100% of species were affected.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.39 no.4
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• pp.423-428
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• 2015

To identify locations and causes of interference among parts of an indoor air-conditioning unit, a 3D tolerance analysis was performed and optimized with respect to assembly gaps and the tolerance of each part. The maximum value of the defect rate resulting from the tolerance analysis was found to be 72.6 at the assembly portion of the body and drain. The maximum displacement caused by the thermal deformation during a heating operation was calculated to be approximately 1 mm by using finite element analysis (FEA). Therefore, it is possible that an interference among the assembled parts occurs. The tolerance of the drain was modified by the results of the sensitivity analysis. As a result, the defect rate was greatly reduced to 0.03. Through the FEA results of the indoor air-conditioning unit, it was shown that the improved tolerance of the drain decreased the interference among the assembled parts even though thermal deformation occurs during operation.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.20 no.8
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• pp.534-540
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• 2008

In this paper, the optimal bang-bang control problem of an air-conditioning system with slab thermal storage was formulated by gradient method. Furthermore, the numeric solution obtained by gradient method algorithm was compared with the analytic solution obtained on the basis of maximum principle. The control variable is changed uncontinuously at the start time of thermal storage operation in an analytic solution. On the other hand, it is showed as a continuous solution in a numeric solution. The numeric solution reproduces the analytic solution when a tolerance for convergence is applied severely. It is conceivable that gradient method is effective in the analysis of the optimal bang-bang control of the large-scale system like an air-conditioning system with slab thermal storage.

• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1119-1125
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• 2024

Phytase increases the availability of phosphate and trace elements by hydrolyzing the phosphomonoester bond in phytate present in animal feed. It is also an important enzyme from an environmental perspective because it not only promotes the growth of livestocks but also prevents phosphorus contamination released into the environment. Here we present a novel phytase derived from Turicimonas muris, TmPhy, which has distinctive structure and properties compared to other previously known phytases. TmPhy gene expressed in the Pichia system was confirmed to be 41 kDa in size and was used in purified form to evaluate optimal conditions for maximum activity. TmPhy has a dual optimum pH at pH3 and pH6.8 and exhibited the highest activity at 70℃. However, the heat tolerance of the wildtype was not satisfactory for feed application. Therefore, random mutation, disulfide bond introduction, and N-terminal mutation were performed to improve the thermostability of the TmPhy. Random mutation resulted in TmPhyM with about 45% improvement in stability at 60℃. Through further improvements, a total of three mutants were screened and their heat tolerance was evaluated. As a result, we obtained TmPhyMD1 with 46.5% residual activity, TmPhyMD2 with 74.1%, and TmPhyMD3 with 66.8% at 80℃ heat treatment without significant loss of or with increased activity.

• Journal of Marine Life Science
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• v.2 no.2
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• pp.83-89
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• 2017

In abalones, interspecific hybridization has been suggested as a possible means to increase production and desired traits for the industry. In Korea, Haliotis gigantea is considered a species with a larger size and higher temperature tolerance than H. discus hannai. However, H. discus hannai is considered the most valuable and popular fishery resource due to its better acceptance and higher market prices. Thus, viable interspecific hybrids have been produced by artificial inseminating H. gigantea eggs with H. discus hannai sperm. However, the reciprocal hybrid cross was not successful. In this study, the hybridity and the growth and thermal tolerance performance of the interspecific hybrids were examined. A combination of various assays revealed maximum growth occurrence at 21℃ and the higher growth rate in the hybrids than that of H. discus hannai parent. In addition, the growth and survival at high-temperature (28℃) of the hybrids was equivalent to that of the highly tolerant H. gigantea parent, suggesting new possibilities to overcome the mass mortality in H. discus hannai during high temperature periods of summer season in Korea. Furthermore, the induced interspecific hybrid status was confirmed by the presence of species-specific bands for each parental species of the random amplified polymorphic DNA (RAPD) profiles using universal rice primer (URP), which could be used as speciesspecific markers to distinguish the hybrids and their parental species.

• Biomolecules & Therapeutics
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• v.5 no.2
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• pp.150-157
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• 1997

Capsaicin cream has been used to attenuate the pain associated with diabetic neuropathy, rheum-atoid arthritis, osteoarthritis and postherpetic neuralgia. But its common side effect, local irritation, limits the use of it and there is still a need for a new analgesic devoid of this side effect. This study was conducted to compare the local irritant effect of DA-5018, a new capsaicin derivative, with that of capsaicin in various animal models and human beings. Capsaicin, applied topically to the mouse ear, produced dose-dependent increase of ear volume and the frequency of ear scratching behavior in mice. Neither ear volume nor scratching behavior was affected by DA-5018. In eye wiping test of rat, DA-5018 was 10 times less irritant than capsaicin. Capsaicin administered intradermally into the rat paw elicited paw lick/lift response with a potency which was three times that of DA-5018. Zostrix-HP (0.075% capsaicin cream), but not DA-50180.3% cream, increased ear volume of rat and induced thermal hyperalgesia in normal and carrageenan inflamed paws. Six day-treatment of Zostrix-HP failed to develop tolerance against this thermal hyperalgesia. In human beings, Zostrix-HP produced burning sensation and itching in more than 90% of volunteers involved and its maximum irritant effect was significantly higher than that of DA-5018 cream. These results suggest that local irritation and burning sensation produced by DA-5018 is much less than capsaicin.

• Journal of Korea Water Resources Association
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• v.46 no.1
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• pp.1-12
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• 2013

In this study, the potential effects of increased water temperature on fish habitat were analysed in the streams of Nakdong River watershed. The changes in suitable habitats for each fish species and in species number at a habitat site were predicted, based on the maximum thermal tolerances of 22 fish species. The estimated maximum thermal tolerance ranged between $27.7^{\circ}C$ and $33.1^{\circ}C$. Then, the increase of water temperature in 78-sites of Nakdong River watershed by 2100 was predicted by using the estimated air temperature data by 2100 in the literature and the regression analysis between air-temperature and water-temperature at each sites. The water temperature was estimated to have increased by $0.69^{\circ}C$, $1.76^{\circ}C$, and $2.32^{\circ}C$ in 2011~2040 (period S1), 2041~2070 (S2), and 2071~2100 (S3), respectively. With such increases in water temperature, the averaged suitable habitats for all 22 fish species would be influenced by 21.9%, 36.3%, and 51.4% in periods S1, S2, and S3, respectively.

• Korean Journal of Agricultural and Forest Meteorology
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• v.14 no.1
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• pp.11-18
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• 2012

Daily temperature data produced by harmonic analysis of monthly climate summary have been used as an input to plant phenology model. This study was carried out to evaluate the performance of the harmonic based daily temperature data in prediction of major phenological developments and to apply the results in improving decision support for agricultural production in relation to the climate change scenarios. Daily maximum and minimum temperature data for a climatological normal year (Jan. 1 to Dec. 31, 1971-2000) were produced by harmonic analysis of the monthly climate means for Seoul weather station. The data were used as inputs to a thermal time - based phenology model to predict dormancy, budburst, and flowering of Japanese cherry in Seoul. Daily temperature measurements at Seoul station from 1971 to 2000 were used to run the same model and the results were compared with the harmonic data case. Leaving no information on annual variation aside, the harmonic based simulation showed 25 days earlier release from endodormancy, 57 days longer period for maximum cold tolerance, delayed budburst and flowering by 14 and 13 days, respectively, compared with the simulation based on the observed data. As an alternative to the harmonic data, 30 years daily temperature data were generated by a stochastic process (SIMMETEO + WGEN) using climatic summary of Seoul station for 1971-2000. When these data were used to simulate major phenology of Japanese cherry for 30 years, deviations from the results using observed data were much less than the harmonic data case: 6 days earlier dormancy release, 10 days reduction in maximum cold tolerance period, only 3 and 2 days delay in budburst and flowering, respectively. Inter-annual variation in phenological developments was also in accordance with the observed data. If stochastically generated temperature data could be used in agroclimatic mapping and zoning, more reliable and practical aids will be available to climate change adaptation policy or decision makers.