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Mating System in Seed Orchard of Japanese Red Pines Revealed by DNA Markers (DNA 표지에 의한 채종원내 소나무 교배양식 구명)

  • Hong, Yong-Pyo;Kim, Young-Mi;Ahn, Ji-Young;Park, Jae-In
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.344-352
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    • 2010
  • To investigate the mating system of clones in the seed orchard of Japanese red pine, parameters of mating system, such as outcrossing rates, number of potential pollen contributors, and degree of pollen contamination, were estimated in the seed orchard of Japanese red pines on the basis of DNA data including 4 nSSR and 6 cpSSR markers. Estimates of outcrossing rates were ranged from 94.9 to 100% with an average of 98.9% on the basis of the analysis of cpSSR haplotypes. They were ranged from 90.3% to 100% with an average of 95.9% on the basis of the analysis of nSSR genotypes. However, cross checking of both DNA markers revealed that the seeds presumed to be products of self pollination were turned out to be generated by pollination between mother tree and other tree (i.e., 100% of cumulative outcrossing rate). Estimates of pollen contamination ranged from 43.6% (Gangwon-10) to 56.4% (Gangwon-12) with the average of 48.9%. On the basis of pooled cpSSR haplotype of each seed, maximum number of 21 pollen contributors were verified from the seeds reproduced by Kyungbuk-38. Minimum number of 13 pollen contributors were verified in Gangwon-10. Mean of 16.2 pollen contributors were verified from a total of 5 mother trees. In conclusion, considering pretty high outcrossing rates between clones within a seed orchard, it may be expected that a fairly good genetic potential of the seeds, produced in '77 plot of the seed orchard of Japanese red pines at Anmyeon island, may be guaranteed. Observed results from the analysis of mating system of Japanese red pines in a '77 plot of the seed orchard may also provide useful information for the establishment and management of the seed orchard of the progressive generation.

Accumulation of Crude Lipids, Phenolic Compounds and Iron in Rusty Ginseng Root Epidermis (적변삼 외피에서 지질, 페놀성물질 및 철 성분의 축적에 관한 연구)

  • Lee, Tae-Su;Mok, Sung-Kyun;Cheon, Seong-Ki;Yoon, Jong-Hyuk;Baek, Nam-In;Choe, Jyung
    • Journal of Ginseng Research
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    • v.28 no.3
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    • pp.157-164
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    • 2004
  • The study was conducted to investigate the tissue and chemical characteristics of rusty root epidermal cells. In histological study, the rusty symptoms were frequently observed in the epidermis of ginseng root and to be yellow under microscopic observation. Disks of the epidermal cell tissue of the rusty root were usually 2 and 3 times greater in the number of cell layer and thickness of cell wall than the healthy root, respectively. The color degree of methanol extracts from the rusty root epidermis was 5.5 times higher than that of the healthy root. And the extracts of rust matter in the root epidermis were easily dissolved in polar solvents compared to nonpolar solvents. UV-absorption spectra of methanol extracts in various fractions of phenolics showed a maximum peak between 275∼280 nm. The crude lipids and phenolic compounds such as acid insoluble bound phenolics, acid insoluble esterified phenolics, acid insoluble condensed phenolics, insoluble bound phenolics and free phenolics were also more in the rusty root epidermis than in the healthy one. Fe content in the rusty root epidermis was 2.7 times higher than that of healthy one. It was presumed that the phenolic compounds(precursor of the rusty) in association with lipid and iron in the root epidermis might defence the root when ginseng root was depressed by the unfavorable conditions in soil and/or portions of a root system were subjected to anoxic conditions.

Quality Characteristics of Bread with Persimmon Peel Powder (감 과피 분말을 첨가한 식빵의 품질특성)

  • Shin, Dong-Sun;Park, Hye-Young;Kim, Myung-Hee;Han, Gwi-Jung
    • Korean journal of food and cookery science
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    • v.27 no.5
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    • pp.589-597
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    • 2011
  • This study was performed to evaluate the physiological and sensory properties of breads with different ratios of persimmon peel. Moisture activation of the bread loaf decreased as storage period increased with a smaller range of decrease in the persimmon peel added group. The amount of weight increased, and volume fell to some degree. Adding persimmon peel increased the RVA temperature, whereas maximum decreased. The color change during storage was due to the addition of control and 4% in group a values except there was no significant difference. As the result of measurements using a texture analyzer, hardness, springiness, cohesiveness, gumminess, and chewiness properties decreased. The sensory test results revealed that the 4% and 6% added persimmon peel bread was the best.

Characteristics of Heavy Metals in the Industrial Complex Area of Pocheon City (포천시 공단지역 미세먼지 중 중금속농도 특성)

  • Shin, Hyung-Soon;Jung, Yeon-Hoon;Kim, Jin-gil;Jung, Jong-Pil;Lee, Sang-Soo;You, Han-Jo;Oh, Jo-Kyo
    • Journal of Environmental Health Sciences
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    • v.45 no.6
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    • pp.577-582
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    • 2019
  • Objectives: The purpose of this survey was to assess the concentrations of heavy metals in the atmosphere of Pocheon City by measuring heavy metals in the industrial complex area and at the city air measuring station, and also to assess the degree of impact that the industrial area has on urban air quality. Methods: Sampling was carried out between February 2018 and November 2018 at two sites in the industrial complex and in the city air monitoring stations. Results: At the industrial complex in Pocheon City, air pollutant emitting businesses were emitting concentrations of fine dust (PM10) between 45 and 60 ㎍/㎥ higher than in the city air. The daily maximum concentrations of lead (Pb), manganese (Mn), and cadmium (Cd) in the industrial complex are below the WHO recommendation standard (annual average), and the impact on the urban atmosphere is judged to be insignificant. Three to five percent of fine dust (PM10) consists of metallic materials, and as the fine dust increased, metals were detected proportionally. Although cadmium (Cd) and beryllium (Be) were not detected in the city air in Pocheon and chromium (Cr), copper (Cu), and arsenic (As) were found to be 50 percent or less, it is deemed that copper (Cu) was detected at unusually high levels due to unknown air pollutants, which requires regular heavy metal measurement and cause verification. Conclusions: An analysis of the heavy metals in the industrial zone and the urban atmosphere in Pocheon City in this study showed that the linear relationship of heavy metals in the industrial zone, or the direct impact relationship, on the heavy metals in the urban atmosphere could not be estimated. The sampling device for equivalent assessment of particulate matter installed at air pollution monitoring stations is highly likely to be used for analysis of fine dust and heavy metals.

Water Absorption, Cooking Properties and Cell Structure of Gamma Irradiated Soybeans (감마선조사 대두의 수분 흡수와 조리특성 및 세포구조)

  • 강일준;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.796-803
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    • 1996
  • Gamma irradiation was applied to soybean(Glycine max.), Hwangkeum, at dose levels of 0, 5, 10 and 20 kGy to improve the physical properties of soybeans. The time to reach a fixed moisture content was reduced depending on the increment of soaking temperatures and applied irradiation dose levels. Irradiation at 5~20 kGy resulted in reduction in soaking time of the soybeans by about 3~6 hrs at soaking temperature of $20^{\circ}C.$ The degree of cooking of soybeans in boiling water was determined by measuring the maximum cutting force of cotyledon. The cutting force to reach complete cooking was about 145g/g. Irradiation at 5~20 kGy resulted in a reduction of cooking time of soybeans by 55~75% as compared to the nonirradiated soybean. In electron microscopic observation of seed coat inner, the parenchyma of nonirradiated soybean showed tight fibrillar structure, whereas that of irradiated soybeans showed loosened and deformed structure. The microstructure of compressed cells and cotyledon epidermis was also deformed by gamma irradiation. In subcellular structure of cotyledon, the roundness of protein body was deformed and changed to spike shape at 20 kGy. Also, the size of lipid body decreased as the irradiation dose levels increased.

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The study for fabrication and characteristic of Li$_2$O-2SiO$_2$conduction glass system using conventional and microwave energies (마이크로파와 재래식 열원을 이용한 고체 전지용 Li$_2$O-2SiO$_2$계 전도성 유리의 제조 및 특성에 관한 연구)

  • Park, Seong-Soo;Kim, Kyoung-Tae;Kim, Byoung-Chan;Park, Jin;Park, Hee-Chan
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.10 no.1
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    • pp.66-72
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    • 2000
  • The behavior of nucleation and crystallization in the $Li_2O_3-SiO_2$ glass heat-treated at different condition under the conventional and microwave processing was studied by differential thermal analysis (DTA), X-ray diffractometry (XRD), optical microscopy (OM), and electrical conductivity measurement. Nucleation temperature and temperature of maximum nucleation rate in both conventionally and microwave heat-treated samples were 460~$500^{\circ}C$ and $580^{\circ}C$, respectively. It was expected that the probability for bulk crystallization increased in microwave heat-treated sample, compared to conventionally heat-treated one. Degree of crystallization increased with increasing crystallization temperature in both conventionally and microwave heat-treated samples. However, pattern of crystallization growth under microwave processing appeared to be quite different from that under the conventional one due to its internal or volumetric heating. Electrical conductivity of conventionally and microwave heat-treated samples were 1.337~2.299, 0.281~~$0.911{\times}10^{-7}\Omega {\textrm}{cm}^{-1}$, respectively.

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Interaction between Flexible Buried Pipe and Surface Load

  • Yoo, Chung-Sik;Chung, Suk-Won;Lee, Kwang-Myung;Kim, Joo-Suk
    • Journal of the Korean Geotechnical Society
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    • v.15 no.3
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    • pp.83-97
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    • 1999
  • This paper presents the results of a parametric study on the interaction between buried pipes and surface load using the finite element method of analysis. A series of laboratory model tests were also performed in order to validate the adopted finite element model and to capture essential features of the physical behavior of buried pipes subjected to surface load. In the parametric study, a wide range of boundary conditions were analyzed with emphasis on the response of the buried pipes to surface load. The results of analysis such as contact stress distribution at the soil/pipe interface and axial thrust of the pipe were thoroughly analyzed, and a database on the response of buried pipe under surface load was established for future development of a semi-empirical design/analysis method. The results indicated that the degree of interaction between buried pipes and surface load significantly varies with the vertical and lateral location of pipe with respect to surface load, and that the current design method, which does not consider soil-structure interaction, cannot correctly capture the pipe response to surface loading. Furthermore, based on the results of analysis, a semi-empirical equation was suggested, which estimates the maximum pipe thrust due to surface load for flexible buried pipes.

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Synthesis and Characterization of Epoxy Silane-modified Silica/Polyurethane-urea Nanocomposite Films (에폭시 변성 실리카 나노입자/폴리우레탄-우레아 나노복합체 필름의 제조 및 특성 연구)

  • Joo, Jin;Kim, Hyeon Seok;Kim, Jin Tae;Yoo, Hye Jin;Lee, Jae Ryung;Cheong, In Woo
    • Korean Chemical Engineering Research
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    • v.50 no.2
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    • pp.371-378
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    • 2012
  • Hydrophilic silica nanoparticles (SNPs) were treated by using 3-glycidoxypropyltrimethoxy silane (GPTMS) and then they were blended with polyurethane-urea (PUU) emulsions to obtain SNPs/PUU nanocomposite films. Thermo-mechanical properties of the nanocomposite films were investigated by varying the grafted amount of GPTMS onto SNPs and the contents of SNPs in the PUU matrix. The thermo-mechanical properties of the nanocomposite films were also compared in terms of the dispersibility of SNPs in the PUU matrix and thermal curing of the GPTMS-grafted SNPs. The maximum amount of grafted GPTMS was $1.99{\times}10^{-6}\;mol/m^2$, and which covered ca. 53% of the total SNP surface area. $^{29}Si$ CP/MAS NMR analyses with the deconvolution of peaks revealed the details of polycondensation degree and patterns of GPTMS in the surface modification of SNPs. The surface modification did not significantly affect colloidal stability of the SNPs in aqueous medium; however, the hydrophobic modification of SNPs offered a favorable effect on the dispersibility of SNPs in the PUU matrix as well as better thermal stability. XRD patterns revealed that GPTMS-grafted SNPs broadened the reduced the characteristic peak of polyol in PUU matrix. The composite films became rigid and less flexible as the SNP content increased from 5 wt.% to 20 wt.%. Particularly, Young's modulus and tensile modulus significantly increased after the thermal curing reaction of the epoxy groups in the SNPs.

Use of Flattening Filter Free Photon Beams for Off-axis Targets in Conformal Arc Stereotactic Body Radiation Therapy

  • Smith, Ashley;Kim, Siyong;Serago, Christopher;Hintenlang, Kathleen;Ko, Stephen;Vallow, Laura;Peterson, Jennifer;Hintenlang, David;Heckman, Michael;Buskirk, Steven
    • Progress in Medical Physics
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    • v.25 no.4
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    • pp.288-297
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    • 2014
  • Dynamic conformal arc therapy (DCAT) and flattening-filter-free (FFF) beams are commonly adopted for efficient conformal dose delivery in stereotactic body radiation therapy (SBRT). Off-axis geometry (OAG) may be necessary to obtain full gantry rotation without collision, which has been shown to be beneficial for peripheral targets using flattened beams. In this study dose distributions in OAG using FFF were evaluated and the effect of mechanical rotation induced uncertainty was investigated. For the lateral target, OAG evaluation, sphere targets (2, 4, and 6 cm diameter) were placed at three locations (central axis, 3 cm off-axis, and 6 cm off-axis) in a representative patient CT set. For each target, DCAT plans under the same objective were obtained for 6X, 6FFF, 10X, and 10FFF. The parameters used to evaluate the quality of the plans were homogeneity index (HI), conformality indices (CI), and beam on time (BOT). Next, the mechanical rotation induced uncertainty was evaluated using five SBRT patient plans that were randomly selected from a group of patients with laterally located tumors. For each of the five cases, a plan was generated using OAG and CAG with the same prescription and coverage. Each was replanned to account for one degree collimator/couch rotation errors during delivery. Prescription isodose coverage, CI, and lung dose were evaluated. HI and CI values for the lateral target, OAG evaluation were similar for flattened and unflattened beams; however, 6FFF provided slightly better values than 10FFF in OAG. For all plans the HI and CI were acceptable with the maximum difference between flattened and unflattend beams being 0.1. FFF beams showed better conformality than flattened beams for low doses and small targets. Variation due to rotational error for isodose coverage, CI, and lung dose was generally smaller for CAG compared to OAG, with some of these comparisons reaching statistical significance. However, the variations in dose distributions for either treatment technique were small and may not be clinically significant. FFF beams showed acceptable dose distributions in OAG. Although 10FFF provides more dramatic BOT reduction, it generally provides less favorable dosimetric indices compared to 6FFF in OAG. Mechanical uncertainty in collimator and couch rotation had an increased effect for OAG compared to CAG; however, the variations in dose distributions for either treatment technique were minimal.

Effect of Light Intensity and Temperature on the Photosynthesis and Respiration of Panax spp (광도와 온도가 인삼의 광합성 및 호흡에 미치는 영향)

  • 이종화
    • Journal of Ginseng Research
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    • v.12 no.1
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    • pp.11-29
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    • 1988
  • This study was conducted to investigate the effect of light intensity and temperature on the photosynthesis and respiration of ginseng plant. Highly significant, second degree curvilinear regressions were recognized among the photosynthesis of ginseng leaves, light intensity and temperature. And an interaction between the effects of light intensity and temperature on the photosynthesis of ginseng leaves was found to be highly significant. The increasing rate of photosynthesis with the increase of light intensity was markedly decreased with increasing temperature. The light compensation point of ginseng leaves was significantly varied with temperature, and the average point was approximately 600 lux. The light saturation point of Korean ginseng was 11,000 lux at $15^{\circ}C$ and $20^{\circ}C$ and around 9,500 lux at above $25^{\circ}C$. The decreasing rate of photosynthesis with the increase of temperature significantly increased with increasing light intensity. The optimum temperature for the photosynthesis of ginseng leaves was about 15 to $22^{\circ}C$ and markedly decreased with increasing light intensity. The highest photosynthesis occurred in ginseng leaves grown with the shade of 15% transmittance. The respiration of ginseng leaves increased with the shade of 5% and/or 30% transmittance. High temperature stimulated the respiration of ginseng leaves. Percent respiration to photosynthesis of ginseng leaves grown with the shade was increased at high temperature and decreased with increasing light Intensity. It was also increased with increasing transmittance. The maximum $CO_2$ absorption of ginseng leaves grown with the shade of 5Ps and ISVS transmittance accurred at 9 o'clock a.m., whereas that of 20% transmittance occurred at 7-9 o'clock a.m. The duration of $CO_2$ absorption was distinctively long with the shade of high transmittance. The $CO_2$ compensation point in the photosynthesis of ginseng leaves was 130 ppm.

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