• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권3호
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• pp.250-265
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• 2008

Distraction osteogenesis is a well-established clinical treatment for limb length discrepancy and skeletal deformities. Appropriate mechanical tension-stress is believed not to break the callus but rather to stimulate osteogenesis. In contrast to fracture healing, the mode of bone formation in distraction osteogenesis is primarily intramembranous ossification. Although the biomechanical, histological, and ultrastructural changes associated with distraction osteogenesis have been widely described, the basic biology of the process is still not well known. Moreover, the molecular mechanisms in distraction osteogenesis remain largely unclear. Recent studies have implicated the growth factor cascade is likely to play an important role in distraction. And current reserch suggested that mechanical tension-stress modulates cell shape and phenotype, and stimulates the expression of the mRNA for bone matrix proteins. The purpose of this study is to examine the pattern of expression of growth factors($TGF-{\beta}1$, IGF-I, bFGF) and extracellular matrix proteins(osteoclacin, osteonectin) related to osteogenesis by osteodistraction of the mandible in rabbits. 24 rabbits is used for this experiment. Experimental group are gradual distraction(0.7mm, twice/day), acute distraction(1.4mm, twice/day) and control group is only osteotomized. After 5 days latency, osteotomic site is distracted for each 7 days and 3.5 days. Consolidation period is 28 days. The animal is sacrificed at the 3th, 7th, 14th, 28th. The distracted bone is examined by immunohistochemical analysis and RT-PCR analysis. The results obtained from this study were as follow : No significant difference was found on clinical examination according to distraction rate, but gradual distraction was shown to improve regenerate bone formation on radiographic and histologic examination. Growth factors and extracelluar matrix proteins expression increased in distraction group than control group. From these results, it could be stated that graudal distraction is shown to improve and accelerate bone formation and mechanical stress like distraction has considerable effects on osteogenesis related factors. And rabbit is the most appropriate animal model for further reseach on the molecular mechanisms that mediate osteodistraction. It is believed that understanding the biomolecular mechanisms that mediate distraction osteogenesis may guide the development of targeted strategies designed to improve distraction osteogenesis and accelerate bone healing.

• 한국산학기술학회논문지
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• 제15권4호
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• pp.1885-1894
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• 2014

본 연구는 다양한 문화콘텐츠 산업과 관련된 조직(기업, 정부 등)이 문화콘텐츠 관련 활동을 수행함에 있어 현재 자국의 수준을 파악하고, 미래 지속 성장을 위한 경영전략을 수립하는데 가이드가 되는 경영전략 수립 모델을 개발하는데 목적이 있다. 이를 위해 본 연구에서는 사용이 편리한 매트릭스 모델을 개발하고자 하였으며, 전문가 대상 FGI를 실시한 결과 '산업의 매력도'와 '기술 강점'의 2개의 축이 기본 측정 요인으로 도출되었으며, 이를 측정하는 세부 구성요인으로는 총 29개의 요인이 도출되었다. 이 세부요인 도출은 선행연구와 문화콘텐츠 산업 전문가를 대상으로 한 2차 설문에 의해 도출되었다. 이 후 도출된 요인들 간의 중요도를 파악하기 위해 AHP분석을 실시, 가중치를 산정하여 현재의 수준을 측정할 수 있는 모델을 제시하였다. 또한, 미래의 지속 성장을 위한 경영전략 가이드 제시를 위해 매트릭스 분면마다 해당 특성을 파악, 이를 정의하고 정부와 기업이 행하여야할 활동을 제시하였다. 본 연구를 통해 우리는 문화콘텐츠 산업만의 특성을 파악할 수 있으며, 지금까지 존재하지 않던 문화콘텐츠 산업의 경영전략 수립 모델을 제시하여, 관련 조직의 경영 활동에 이바지하고자 한다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제28권6호
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• pp.456-463
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• 2002

Distraction osteogenesis is a well-established clinical treatment for limb length discrepancy and skeletal deformities. Appropriate mechanical tension-stress is believed not to break the callus but rather to stimulate osteogenesis. In contrast to fracture healing, the mode of bone formation in distraction osteogenesis is primarily intramembranous ossification. Although the biomechanical, histological, and ultrastructural changes associated with distraction osteogenesis have been widely described, the basic biology of the process is still not well known. Moreover, the molecular mechanisms in distraction osteogenesis remain largely unclear. Recent studies have implicated the growth factor cascade is likely to play an important role in distraction. And current reserch suggested that mechanical tension-stress modulates cell shape and phenotype, and stimulates the expression of the mRNA for bone matrix proteins. This article presents the hypotheses and current research that have furthered knowledge of the molecular biology that govern distraction osteogenesis. The gene regulation of growth factors and extracellular matrix proteins during distraction osteogenesis are discussed in this article. It is believed that understanding the biomolecular mechanisms that mediate distraction osteogenesis may guide the development of targeted strategies designed to improve distraction osteogenesis and accelerate bone healing.

• 생체재료학회지
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• 제22권4호
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• pp.337-345
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• 2018

Background: Human mesenchymal stem cells (hMSCs) are, due to their pluripotency, useful sources of cells for stem cell therapy and tissue regeneration. The phenotypes of hMSCs are strongly influenced by their microenvironment, in particular the extracellular matrix (ECM), the composition and structure of which are important in regulating stem cell fate. In reciprocal manner, the properties of ECM are remodeled by the hMSCs, but the mechanism involved in ECM remodeling by hMSCs under topographical stimulus is unclear. In this study, we therefore examined the effect of nanotopography on the expression of ECM proteins by hMSCs by analyzing the quantity and structure of the ECM on a nanogrooved surface. Methods: To develop the nanoengineered, hMSC-derived ECM, we fabricated the nanogrooves on a coverglass using a UV-curable polyurethane acrylate (PUA). Then, hMSCs were cultivated on the nanogrooves, and the cells at the full confluency were decellularized. To analyze the effect of nanotopography on the hMSCs, the hMSCs were re-seeded on the nanoengineered, hMSC-derived ECM. Results: hMSCs cultured within the nano-engineered hMSC-derived ECM sheet showed a different pattern of expression of ECM proteins from those cultured on ECM-free, nanogrooved surface. Moreover, hMSCs on the nano-engineered ECM sheet had a shorter vinculin length and were less well-aligned than those on the other surface. In addition, the expression pattern of ECM-related genes by hMSCs on the nanoengineered ECM sheet was altered. Interestingly, the expression of genes for osteogenesis-related ECM proteins was downregulated, while that of genes for chondrogenesis-related ECM proteins was upregulated, on the nanoengineered ECM sheet. Conclusions: The nanoengineered ECM influenced the phenotypic features of hMSCs, and that hMSCs can remodel their ECM microenvironment in the presence of a nanostructured ECM to guide differentiation into a specific lineage.

• 농업과학연구
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• 제39권1호
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• pp.125-132
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• 2012

The 2009 Agricultural Survey is one of the most important official statistics about agricultural sector, which is mostly used by experts. However users have kept claiming about the problems of the 2009 Agricultural Survey, which need to be discussed in the academic area. The purpose of this paper is to analyze the 2009 Agricultural Survey and the level of satisfaction of users and to evaluate the 2009 Agricultural Survey. Then, the paper presents some suggestions to upgrade the 2009 Agricultural Survey. The results of studies present a few findings: First, the 2009 Agricultural Survey shows overall excellence but should focus on adding more various topics in agricultural sector, Second, the 2009 Agricultural Survey need to consider offering micro level data for experts such as data in the city or county level, Third, the 2009 Agricultural Survey had better to offer more specific examination and guide line for general users.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2002년도 춘계학술대회 논문집
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• pp.480-485
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• 2002

The purpose of this paper is the development of guidance algorithm for a mobile robot which is used to acquire the position and state information of the pipe defects such as crack, damage and through hole. The data used for the algorithm is the range data obtained by the range sensor which is based on an optical triangulation method. The sensor, which consists of a laser slit beam and a CCD camera, measures the 3D profile of the pipe's inner surface. After setting the range sensor on the robot, the robot is put into a pipe. While the camera and the LSB sensor part is rotated about the robot axis, a laser slit beam (LSB) is projected onto the inner surface of the pipe and a CCD camera captures the image. From the images the range data is obtained with respect to the sensor coordinate through a series of image processing and applying the sensor matrix. After the data is transformed into the robot coordinate, the position and orientation of the robot should be obtained in order to guide the robot. In addition, analyzing the data, 3D shape of the pipe is constructed and the numerical data for the defects of the pipe can be found. These data will be used for pipe maintenance and service.

• International Journal of Quality Innovation
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• 제9권2호
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• pp.79-89
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• 2008

Each amusement park has a wayfinding system, while symbols are important mediums to guide tourists to find their destinations. It is very important that whether the meanings of symbols recognized by tourists immediately. This paper mainly discusses the recognition of graphic symbols in amusement park, and proposes the improvement suggestions. Materials for this study were drawn from 20 different graphic symbols of a theme amusement park in Taiwan. The testees were required to evaluate the design of graphic symbols based on symbolic meaning and graphics recognition to summarize the confusion matrix. The results show that there are three groups of graphic symbols easy to be confused, and five symbols not meeting a criterion of 67% correct responses. The reasons were discussed, and improvement and relevant suggestions have been proposed, which may be helpful to redesign of symbols.

• 설비공학논문집
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• 제25권7호
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• pp.363-370
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• 2013

HVAC&R systems are the most energy consuming building services, representing approximately half of the final energy use in the building sector. Despite their significant energy use, there is a lack of a consistent and homogeneous framework to efficiently guide research. This research is about the energy consumptions of HVAC&R systems, with the aim of establishing a common idea for the analysis of building energy efficiency. Our approach deals with the concept of an HVAC&R set that is composed of subsystems. A matrix combination evaluation is examined, and a total of 960 sets can be implemented in a large scale office building. A method as a tool for energy evaluations of HVAC&R systems, and HEET as a energy simulation program, were developed.

• KSBB Journal
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• 제32권1호
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• pp.54-62
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• 2017

This study aimed to estimate the measurement uncertainty related to determination of hyaluronic acid used as a dietary supplement by high-performance liquid chromatography. According to the guidelines of the Association of Official Analytical Chemists, the analytical processes for determination of hyaluronic acid were performed. And the measurement uncertainty obtained during the analytical processes were expressed in accordance with mathematical/ statistical guidances of GUM (Guide to the Expression of Uncertainty in Measurement) & EURACHEM (Focus for Analytical Chemistry in Europe) for the analytical operations. For the uncertainty in measurement produced based on this analytical method, the expanded uncertainty was calculated by using the relative standard uncertainty between analytical results and sources of uncertainty in measurement (sample weight, final volume, extraction volume, standard solution, matrix and instrument etc). In the results of 95% confidence interval, it was calculated that the uncertainty in measurement was $57.75{\pm}8.76{\mu}g/kg$ (k=2.0). Therefore, it showed that the measurement uncertainty obtained by this analytical method influences on 15.2% of the contents of hyaluronic acid as the analytical results.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2009년도 춘계학술발표대회
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• pp.15.2-15.2
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• 2009

Manufacturing of printed electronics using printing technology has begun to get into the hot issue in many ways due to the low cost effectiveness to existing semi-conductor process. This technology with both low cost and high productivity, can be applied in the production of organic thin film transistor (OTFT), solar cell, radio frequency identification (RFID) tag, printed battery, E-paper, touch screen panel, black matrix for liquid crystal display (LCD), flexible display, and so forth. The emerging technology to manufacture the products in mass production is roll-to-roll printing technology which is a manufacturing method by printings of multi-layered patterns composed of semi-conductive, dielectric and conductive layers. In contrary to the conventional printing machines in which printing precision is about $50~100{\mu}m$, the printing machines for printed electronics should have a precision under $30{\mu}m$. In general, in order to implement printed electronics, narrow width and gap printing, register of multi-layer printing by several printing units, and printing accuracy of under $30{\mu}m$ are all required. We developed the roll-to-roll printing equipment used for printed electronics, which is composed of un-winder, re-winder, tension measurement system, feeding units, dancer systems, guide unit, printing unit, vision system, dryer units, and various auxiliary devices. The equipment is designed based on cantilever type in which all rollers except printing ones have cantilever types, which could give more accurate machine precision as well as convenience for changing rollers and observing the process.