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The complete chloroplast genome of Scrophularia kakudensis and a comparative analysis of S. kakudensis and S. cephalantha

  • Ogyeong SON;KyoungSu CHOI
    • Korean Journal of Plant Taxonomy
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    • v.53 no.3
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    • pp.237-241
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    • 2023
  • The genus Scrophularia L. (Scrophulariaceae) comprises 200-270 species worldwide and is a taxonomically challenging lineage, displaying morphological diversity and hybridization. S. kakudensis is morphologically similar to the closely related taxa S. kakudensis var. microphylla, S. pilosa, and S. cephalantha. Therefore, the purpose of this study was to sequence the chloroplast (cp) genome of S. kakudensis using next-generation sequencing and compare it to those of related taxa. The complete cp genome sequence of Scrophularia kakudensis was found to be 152,355 bp long, consisting of a pair of inverted repeats of 25,485 bp that separate a large single-copy (LSC) of 83,479 bp from small single-copy regions of 17,909 bp. The cp genome contained 78 protein-coding genes, 30 tRNAs, and four rRNAs. A phylogenetic analysis based on 78 protein-coding genes from six Scrophularia species showed S. kakudensis and S. cephalantha formed with 100% bootstrap values. We compared the complete cp genomes of S. kakudensis and S. cephalantha and identified seven sequence divergence regions: matK/rps16, rps16/trnQ, trnS/trnG, rpoB/trnC, trnS/trnG, rpl32/trnL, and ndhD/psaC. These regions may be useful for determining the phylogenetic relationships among S. kakudensis-related species.

Synthesis of PSZ-seeding Mullite Composite from Metal Alkoxides and Its Characteristics of Sintered Body (금속 알콕사이드로부터 PSZ-seeding Mullite 복합체의 합성 및 소결체의 특성)

  • Yim, Going;Yim, Chai-Suk;Kim, Young-Ho
    • Korean Journal of Materials Research
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    • v.17 no.1
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    • pp.18-24
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    • 2007
  • Mullite-PSZ composite was prepared by sol-gel method using $Al(sec-OC_4H_9)_3,\;Si(OC_2H_5)_4,\;ZrOCl_2\;8H_2O\;and\;Y_2O_3$. The sinterability ana mechanical properties of powder compacts sintered at $1,650^{\circ}C$ for 4 hrs were investigated for various PSZ contents. In result Al-Si spinel formed at $980^{\circ}C$ from amorphous dried gel, and zirconia as well as mullite crystal formed above $1,200^{\circ}C$. The sintered body was densified to $97{\sim}98%$ except the specimen containing 25vol% PSZ which showed the relative density of about 95% obtained by sintering at $1,650^{\circ}C$ for 4 h. The flexural strength of the sintered body was a maximum value of 290 MPa in 20 vol% PSZ, which was also considerably larger than the value of 200 MPa without PSZ. The value of the fracture toughness increased linearly with increase of PSZ content and showed a maximum value of $4.3MPam^{1/2}$ in 25 vol% PSZ, Namely this value was remarkably larger than the $value(2.6MPam^{1/2})$ of pure mullite without PSZ.

Ergonomic Evaluation of Trunk-Forearm Support Type Chair

  • Lim, Seung Yeop;Won, Byeong Hee
    • Journal of the Ergonomics Society of Korea
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    • v.33 no.2
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    • pp.143-153
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    • 2014
  • Objective: The aim of this study is to investigate the effects of trunk-forearm supported sitting on trunk flexion angle, trunk extensor fatigue and seat contact pressure. Background: The relationship between sitting posture and musculoskeletal disorders of the trunk extensor fatigue and seat contact pressure has been documented. The trunk-forearm support type ergonomic chair was devised from the fact that trunk-forearm support has been reported to reduce trunk extensor activity and discomfort. Method: Using three different sitting postures, upright ($P_1$), trunk-forearm supported ($P_2$) and normal sitting ($P_3$), six healthy subjects participated in the study. Motion capture system was used to collect head and trunk flexion angle, and surface electromyography (sEMG) was used to collect myoelectric signal of upper trapezius, lower trapezius, erector spinae, multifidus, and pressure mat system was used to measure seat contact pressure. Results: When trunk and forearm were supported by the ergonomic chair, higher head flexion angle showed upright > trunk-forearm supported > normal in order, and muscle fatigue showed less than upright and normal sitting. Mean seat contact pressure decreased 19% than upright sitting. But muscle fatigue was not affected by each condition. Conclusion: Trunk-forearm supported sitting of the ergonomic chair showed positive effect in respect of trunk and head flexion angle, trunk extensor fatigue, seat contact pressure. To acquire comprehensive understanding of the effectiveness of the ergonomic chair, further studies such as anatomical effects from measurement of external applied loading effect to the body from interface pressure analysis are required. Application: The results of the publishing trend analysis might help physiological effects of trunk-forearm support type chair.

Expression of Recombinant Korean Mistletoe(KM) Lectin and B genes in Saccharomyces cerevisiae (Saccharomyces cerevisae에서 한국산 겨우살이 유래 lectin A 및 B 유전자의 발현)

  • 최윤혁;김종배;양웅석;황철원
    • Journal of Life Science
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    • v.14 no.5
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    • pp.840-846
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    • 2004
  • A study for expression of Korean Mistletoe (KM) lectin gene (A,B) in Saccharomyces cerevisiae was done using transforming system of yeast. In order to overexpress the genes efficiently in yeast, two lectin genes (A,B) were re-cloned and modified including Kozak translation initiation sequence using PCR amplification. The constructed plasmids containing modified lectin A and B genes were transformed to S. cerevisea INVSc (MAT G, his3 $\Delta$1, leu2, trpl-289, ura3-52). The transformed cells were identified by DNA sequencing with ABI3700 system and induced with 2% of galactose for recombinant KM lectin (rKM lectin) protein. The rKM lectin A and B proteins were determinated about 29kDa size of protein by SOS-P AGE and western blotting analysis. The expressed recombinant lectin was determinated 1.24∼1.75 $\mu\textrm{g}$ per 1 mg of cytosolic soluble protein by sandwich ELISA method. Moreover the lectin genes were expressed as maximum level at 36 h after galactose induction and lectin A gene was were repressed after 48 h.

Changes in Breast-tumor Blood Flow in Response to Hypercapnia during Chemotherapy with Laser Speckle Flowmetry

  • Kim, Hoonsup;Lee, Youngjoo;Lee, Songhyun;Kim, Jae Gwan
    • Current Optics and Photonics
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    • v.3 no.6
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    • pp.555-565
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    • 2019
  • Development of a biomarker for predicting tumor-treatment efficacy is a matter of great concern, to reduce time, medical expense, and effort in oncology therapy. In a preclinical study, we hypothesized that the blood-flow parameter based on laser speckle flowmetry (LSF) could be a potential indicator to estimate the efficacy of breast-cancer treatment. To verify this hypothesis, a 13762-MAT-B-III rat breast tumor was grown in a dorsal skinfold window chamber applied to a nude mouse, and the change in blood flow rate (BFR) - or the speckle flow index (SFI) is used together as the same meaning in this manuscript - was longitudinally monitored during tumor growth and metronomic cyclophosphamide treatment. Based on the daily LSF angiogram, several BFR parameters (baseline SFI, normalized SFI, and △rBFR) were compared to tumor size in the normal, treated, and untreated tumor groups. Despite the incomplete tumor treatment, we found that the daily changes in all BFR parameters tended to have partially positive correlation with tumor size. Moreover, we observed that the changes in baseline SFI and normalized SFI responded one day earlier than the tumor shrinkage during chemotherapy. However, daily variations in the hypercapnia-induced △rBFR lagged tumor shrinkage by one day. This study would contribute not only to evaluating tumor vascular response to treatment, but also to monitoring blood-flow-mediated diseases (in brain, skin, and retina) by using LSF in preclinical settings.

Formation and Microstructure Characteristics of $\beta-Al_5FeSi$ Intermetallic Compound in the Al-Si-Cu Alloys with the Variation of Fe Content (Al-Si-Cu합금에서 Fe 함량에 따른 $\beta-Al_5FeSi$ 금속간화합물의 형성 및 응고미세조직 특성)

  • Kim, Bong-Hwan;Lee, Sang-Mok
    • Journal of Korea Foundry Society
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    • v.29 no.5
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    • pp.225-232
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    • 2009
  • For comprehensive understanding of the formation behavior of $\beta-Al_5FeSi$ phase in Al-Si-Cu alloys with the existence of Fe element, microstructure characterizations were performed using combined analysis of OM, SEM-EDS, XRD. Especially, experimental and predictive works on solidification events of $\beta-Al_5FeSi$ phase as well as other phases formed together with $\beta-Al_5FeSi$ have been carried out by using DSC analysis and Java-based Materials Properties software (J. Mat. Pro.). Primary and eutectic $\beta-Al_5FeSi$ phases were able to distinguish from each other on microstructures by their morphological features. Primary $\beta-Al_5FeSi$ phase was seen to have rough surface perpendicular to growth direction, indicating free attachment of solute atoms in liquid state. On the other hand, the eutectic $\beta-Al_5FeSi$ phase was formed with plain and straight surface during eutectic reaction together with $\alpha$-Al phase. The eutectic reaction of $\beta-Al_5FeSi$ and $\alpha$-Al phases was seen to be able to separate into each formation depending on cooling rate.

Analysis on the Scour Reduction Effect by Controlling Downflow (하강류 제어를 통한 교각세굴 감소 효과 분석)

  • Lee, Ho Jin;Oh, Hyoun Sik
    • Journal of Korean Society of Disaster and Security
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    • v.11 no.2
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    • pp.61-67
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    • 2018
  • Scour protection methods can be categorized as two types: The first is to reduce the horseshoe and wake vortices which are the main reasons for local scour. Either small cylindrical structures or separated vertical deflectors can be placed in front of the pier or the horizontal deflector (or collar) can be attached to the pier like the spoiler to reduce the dynamics of vortical structures. The second is to employ the protection layer to keep the bed material in place, which is a common method with a merit of immediate effect by using block mat or tetrapod. This study examined the effect of scour reduction using the former method. The relationship between the reflector interval and reduction of scour was not clear. It is assumed that the width of the reflector is somewhat correlated with the reduction of the scour. As the KC numbers increases, the Effect of Scour reduction rate is shown to decrease. Also, Scour reduction rate showed a rapid change at $U_R=25$ or KC = 8.

Brown Felt on Persimmon (Diospyros kaki) Caused by Septobasidium sp. in Korea (Septobasidium sp.에 의한 감 갈색고약병 발생)

  • Ha, Jeong-Seok;Song, In-Kyu;Kim, Seung-Han;Kim, Ji-Won
    • Research in Plant Disease
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    • v.27 no.2
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    • pp.66-69
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    • 2021
  • In 2020, a brown felt was observed on persimmon (Diospyros kaki) in persimmon orchards, Sangju, Korea. The symptom on persimmon was white to grey mycelial mats on some areas of the branches. Each mat progressively expanded until the mats coalesced to occupy larger areas and finally girdled the branches. The disease branches were covered with brown-colored mold, consisting of hyphal mats of the pathogen. Optimum temperature for mycelial growth was 30℃. On the basis of mycological characteristics, pathogenicity test, and molecular analysis with complete internal transcribed spacer rDNA region, the causal fungus was identified as Septobasidium sp. This is the first report of brown felt caused by Septobasidium sp. on persimmon in Korea.

A computational estimation model for the subgrade reaction modulus of soil improved with DCM columns

  • Dehghanbanadaki, Ali;Rashid, Ahmad Safuan A.;Ahmad, Kamarudin;Yunus, Nor Zurairahetty Mohd;Said, Khairun Nissa Mat
    • Geomechanics and Engineering
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    • v.28 no.4
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    • pp.385-396
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    • 2022
  • The accurate determination of the subgrade reaction modulus (Ks) of soil is an important factor for geotechnical engineers. This study estimated the Ks of soft soil improved with floating deep cement mixing (DCM) columns. A novel prediction model was developed that emphasizes the accuracy of identifying the most significant parameters of Ks. Several multi-layer perceptron (MLP) models that were trained using the Levenberg Marquardt (LM) backpropagation method were developed to estimate Ks. The models were trained using a reliable database containing the results of 36 physical modelling tests. The input parameters were the undrained shear strength of the DCM columns, undrained shear strength of soft soil, area improvement ratio and length-to-diameter ratio of the DCM columns. Grey wolf optimization (GWO) was coupled with the MLPs to improve the performance indices of the MLPs. Sensitivity tests were carried out to determine the importance of the input parameters for prediction of Ks. The results showed that both the MLP-LM and MLP-GWO methods showed high ability to predict Ks. However, it was shown that MLP-GWO (R = 0.9917, MSE = 0.28 (MN/m2/m)) performed better than MLP-LM (R =0.9126, MSE =6.1916 (MN/m2/m)). This proves the greater reliability of the proposed hybrid model of MLP-GWO in approximating the subgrade reaction modulus of soft soil improved with floating DCM columns. The results revealed that the undrained shear strength of the soil was the most effective factor for estimation of Ks.

Nouvelle Dramaturgie et limites du Théâtre postmoderne à travers les pièces de Michel Vinaver (포스트모던 연극의 새로운 극작술과 그 한계 : 미셀 비나베르(Michel Vinaver)의 작품을 중심으로)

  • Ha, Hyung-Ju
    • Journal of Korean Theatre Studies Association
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    • no.53
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    • pp.203-233
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    • 2014
  • Depuis les $ann{\acute{e}}es$ 1990, sous l'influence de la $pens{\acute{e}}e$ postmoderne, des ${\oe}uvres$ $d{\acute{e}}constructivistes$ ont fait leur apparition dans le $th{\acute{e}}{\hat{a}}tre$ $cor{\acute{e}}en$. Cependant, cette approche est plus remarquable dans le domaine du $th{\acute{e}}{\hat{a}}tre$ pratique que dans celui de $l^{\prime}{\acute{e}}criture$ dramatique. Par exemple, ce mouvement a $commenc{\acute{e}}$ avec les metteurs en $sc{\grave{e}}ne$ OH Tae-suk et GUI Gook-seo au $d{\acute{e}}but$ des $ann{\acute{e}}es$ 1990. Leur ont $succ{\acute{e}}d{\acute{e}}$ ensuite les metteurs en $sc{\grave{e}}ne$ CHOI Yong-hoon, de la troupe de $th{\acute{e}}{\hat{a}}tre$ Chak Eun Shin Hwa, et PARK Kun-hyung. De plus, actuellement, de jeunes metteurs en $sc{\grave{e}}ne$ travaillent avec $perspicacit{\acute{e}}$, tels que KO Sung-woong, KANG Lyang-won et YOUN Han-sol. A $l^{\prime}oppos{\acute{e}}$ de ce $th{\acute{e}}{\hat{a}}tre$ pratique, $l^{\prime}{\acute{e}}criture$ dramatique des $pi{\grave{e}}ces$ de $th{\acute{e}}{\hat{a}}tre$ demeure au niveau de $l^{\prime}{\acute{e}}criture$ $repr{\acute{e}}sentative$ et est encore sans grande valeur. Pour $r{\acute{e}}fl{\acute{e}}chir$ ${\grave{a}}$ ce $probl{\grave{e}}me$, nous $consid{\acute{e}}rerons$ les $pi{\grave{e}}ces$ de Michel Vinaver dont la dramaturgie oppose $l^{\prime}{\acute{e}}criture$ $d{\acute{e}}constructiviste$ au principe $repr{\acute{e}}sentatif$ et ${\grave{a}}$ $l^{\prime}{\acute{e}}criture$ traditionnelle en ${\acute{e}}chappant$ au principe de $causalit{\acute{e}}$. Avec sa $strat{\acute{e}}gie$ de montage $sp{\acute{e}}cifique$, il $proc{\grave{e}}de$ avec une ${\acute{e}}criture$ $fragment{\acute{e}}e$ tout en $ins{\acute{e}}rant$ des dialogues $h{\acute{e}}t{\acute{e}}rog{\grave{e}}nes$. Il trouve ainsi le silence des mots, un $pr{\acute{e}}sent$ sensible, la $mat{\acute{e}}rialit{\acute{e}}$ des mots et des images ostensives ${\grave{a}}$ travers des dialogues $juxtapos{\acute{e}}s$, une $simultan{\acute{e}}it{\acute{e}}$ et une $discontinuit{\acute{e}}$ du dialogue. En examinant ses $pi{\grave{e}}ces$ de $th{\acute{e}}{\hat{a}}tre$, nous explorons cette nouvelle ${\acute{e}}criture$ qui $r{\acute{e}}siste$ au $r{\acute{e}}gime$ dramatique de la $repr{\acute{e}}sentation$. Toutefois, nous n'encenserons pas seulement cette ${\acute{e}}criture$ postmoderne. Plus $pr{\acute{e}}cis{\acute{e}}ment$, nous $rel{\grave{e}}verons$ ses limites, dans les circonstances $pr{\acute{e}}sentes$, en $r{\acute{e}}fl{\acute{e}}chissant$ aux limites du $th{\acute{e}}{\hat{a}}tre$ postmoderne depuis le $d{\acute{e}}but$ des $ann{\acute{e}}es$ 2000. Ce faisant, nous souhaitons $d{\acute{e}}finir$ de nouvelles $possibilit{\acute{e}}s$ $esth{\acute{e}}tiques$.