• Title/Summary/Keyword: Mastoparan B

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Interaction of Hornet Venom and its Derivatives in the Phospholipid Membrane Environment (말벌 독성 물질과 그 유도체의 인지질 막 환경에서의 상호작용)

  • 이봉헌;박홍재
    • Journal of Environmental Science International
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    • v.7 no.1
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    • pp.62-66
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    • 1998

  • Toxic Mastoparan B(MP-B) which is purified from the venom of the hornet Vespa basalis is a cationic amphlphilic tetradecapeptide. MP-B and Its Ala-substituted analogues were synthesized by solld phase method and the toxic peptide-membrane interactions were examined by circular dichroism(CD) spectra, fluorescence spectra, and leakage abilities in phospholipid membranes. In the presence of phospholipid vesicles, synthetic MP-B and its analogues formed amphiphilic -helical structures, but in the buffer soletion, those exhibited random coil conformation as measured by CD. Fluorescence spectra of MP-B and its analogues which indicated the binding affinity of peptide on phospholipid vesicles showed that the replacement of Lys at position 2 and 11 with Ala caused a remarkable effect in the blue shalt and that at position 2, in the leakage ability of the peptide.

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