• 제목/요약/키워드: Masson's trichrome stain

검색결과 46건 처리시간 0.028초

Tissue Conditioner가 구강조직에 미치는 영향에 관한 실험적 연구 (An Experimental Study on the Effect of Tissue Conditioner on the Oral Mucosa)

  • 김창희
    • 대한치과의사협회지
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    • 제17권9호통권124호
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    • pp.695-700
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    • 1979
  • An experimental study was performed to study the tissue reactions to tissue conditioners in rabbits. Thirty rabbits were divided into three groups, 10, Hydro-cast group, 10, Coe-comfort group, and 10, heat-cured resin group. Tissue conditioners and heat-cured resin were embedded in the oral mucosa of rabbits. The tissue specimens were removed on 3rd, 7th, 14h, 21st and 28th day after embedding and examined under microscope after staining them with H-E stain, Van Gieson's stain, Masson's trichrome stain and PAS reaction. The results were as follows : 1. Tissue reactions to tissue conditioners were somewhat different from each other in the early stage, but, with the increase of the embedding period, the fibrous capsule was thickened in both. These tissue reactions were similar to those to heat-cured resin. 2. Newly formd fibrous components were stained deep-red with Van Gieson's stain and dark-green with Masson's trichrome stain. But their stainability was decreased as collagenous fibers became matured. 3. Newly formed fibrous components showed intense PAS reactivity, but PAS reactivity was reduced as the connective tissue capsule became completed.

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미세전류자극과 고전압 맥동직류 통전이 토끼의 비골 골절치유에 미치는 효과 (Effects of Microcurrent and High Voltage Pulsed Galvanic Current Stimulation on Fibular Fracture Healing of the Rabbits)

  • 고승현;윤범철;김지성;민경옥
    • 한국콘텐츠학회논문지
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    • 제11권10호
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    • pp.286-292
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    • 2011
  • 본 연구는 토끼골절모델에 미세전류자극(직류, 음극), 고전압 맥동직류 음극과 양극을 이용해 골절치유정도를 살펴보았다. 방사선 검사에 의한 육안 계측은 미세전류자극군이 고전압 맥동직류의 음극과 양극 통전군보다 골절치유척도 점수에서 통계학적으로 유의한 차이를 보였으나(p<0.05), 고전압 맥동직류 음극군과 양극군 사이에는 유의한 차이가 없었다(p>0.05). Hematoxylin-Eosin 염색과 Masson's trichrome 염색을 통한 병리조직표본 차이는 미세전류자극군에서 무층골의 증식이 다른 두 실험군보다 더 활발하게 관찰되었으며 연골내골화 과정도 다른 두 실험군에 비해 더 빠른 것으로 관찰되었다. Osteocalcin 면역조직화학 염색은 미세전류자극군이 골모세포, 골세포, 파골세포 및 골기질 내에서 면역양성반응이 다른 두 실험군보다 더 명확히 관찰되었다.

흰쥐의 화상부위에 시술된 침과 소염약침이 조직회복에 미치는 영향 (Effects of the Acupuncture or Pharmacopunture Treatment for the Histologic and Morphologic Recovery on the Mouse with the Burn Skin)

  • 송범용;이창현
    • Journal of Acupuncture Research
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    • 제26권3호
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    • pp.81-92
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    • 2009
  • Objectives: Burn is a severe dermal injury caused by heat. We were to investigated the effects of acupuncture or pharmacopunture treatment for the histologic and morphologic recovery on the mouse with the 3rd grade burn skin. Methods : We divided into 3 groups. One was a control group(n=3) that was not treated any treatments. Another was a acupuncture group(n=3) that was treated only acupuncture. The other was a pharmacopuncture group(n=3) that was treated only pharmacopuncture. We made a 3rd grade burned skin with the stainless steel heating apparatus. We made a treatment for the mice for a week(2 times for a day, totally 14 times treatments). We observed a dermal morphologic recovery on the mice and a histopathological photographs of the burn skin and subcutaneous tissue with H&E stain, Masson's trichrome stain, and VEGF, FGF and c-kit immunohistochemical stain. Results : The pharmacopuncture group were a better morphologic recovery than control group and acupuncture group. And the pharmacopuncture group were a better histopathological recovery than control group and acupuncture group on the burn skin and subcutaneous tissue with H&E stain, Masson's trichrome stain. And the pharmacopuncture group were a better histopathological recovery than control group and acupuncture group on the burn skin and subcutaneous tissue with VEGF, FGF and c-kit immunohistochemical stain. Conclusions : We suggest that the pharmacopuncture treatment is a better histologic and morphologic recovery than the no treatment or the acupuncture group with the burned mouse skin.

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실험적 치아 이동시 성견 치주조직의 변화에 대한 면역조직화학적 연구 (IMMUNOHISTOCHEMICAL STUDY ON THE PERIODONTAL TISSUE REACTION DURING EXPERIMENTAL TOOTH MOVEMENT IN THE ADULT DOG)

  • 김미정;양원식
    • 대한치과교정학회지
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    • 제23권1호
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    • pp.89-100
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    • 1993
  • The purpose of this study was to evaluate the effect of orthodontic force on periodontal cellular activity by immunoperoxidase stain of epidermal growth factor, one of the tissue hormone. And supplementarily, to investigate of the changes of periodontal structures, periodontium was stained by H-E, Masson's Trichrome, P. A. S. stain after orthodontic force application. The experimental animals were four young adult dogs of average 8 month old. The fixed orthodontic appliance was cemented on mandibular right 4th premolar and 1st molar of each animal as experimental site. Mandibular left 4th premolar area of the same animal was used as control. The appliance consist of two silver crown soldered with 0.030' tube, $0.018\times0.022'$ S.S. sectional arch wire, and 0.009' open coil spring for manifestating of orthodontic force for bodily tooth movement of mandibular 4th premolar toward mesial direction. Experimental group was sacrificed at 1, 2, 3, 5 weeks from beginning of the experiment, and was investigated immunohistochemically and bistochemically by several staining methods. Findings were as follows: 1. The degree of EGF staining in control group was highest in epithelium of periodontium, and osteoclasts, osteoblasts and fibroblasts around the capillary were stained at higher level in periodontium. Generally, control group shows positive distribution of EGF all around the periodontal area. 2. The degree of EGF staining in control and 5 week group were similar, and did not show the significant different level between tension and pressure side. 3. All of 1, 2, 3 week group showed the same staining degree and distribution of EGF, and the tension side was more positive reaction of EGF stain than the pressure side. 4. The features of collagen fiber and periodontal fiber arrangement observed by H-E, Masson's Trichrome and P. A. S. stain revealed that oblique periodontal fibers were strectched in tension side, compressed in pressure side of all experimental group. Some fiber group in pressure side of 5 week group recovered the regular arrangement along the capillaries.

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유성견 소구치 정출시 치아 및 치주 조직의 초기 조직학적 변화 및 재생에 관한 연구 (A HISTOLOGIC STUDY OF INITIAL CHANCE AND REPAIR OF TOOTH AND PERIODONTAL TISSUE IN EXTRUSION OF YOUNG ADULT DOGS)

  • 윤병선;차경석;이진우
    • 대한치과교정학회지
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    • 제28권3호
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    • pp.419-429
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    • 1998
  • 본 실험은 유성견에서 인위적인 정출 시 조직학적, 임상학적인 반응과 시간경과에 따른 치아 및 치아 주위조직의 회복을 연구하기 위해 생후 평균 10 개월 웅성 잡종 유성견 하악 소구치를 대상으로 Energy chain을 이용한 정출 실험으로, 실험 1 군은 힘을 작용 시킨 후 일 주일만에 희생시키고 실험 2 군은 정출력을 가한 후 일 주일 후, 정출력이 제거되고 3 주간의 회복 기간 경과 후에 희생시켰다. HE 및 Masson's Trichrome 염색법을 이용하여 치아와 주위 조직의 반응을 치경부, 치근 중앙부, 치근단부, 치수부로 분류하여 광학 현미경하에서 관찰하여 다음과 같은 결과를 얻었다. 1. 힘을 가한 후 일 주일 후, 모든 부위에서 치주인대의 주행방향이 치아를 향하여 사선 방향을 나타내 주었으며 치주인대의 간격이 전반적으로 증가되었다. 또 부분적인 치주 인대의 파열이 치근단부에서 뚜렷하게 관찰되었다. MT 소견에서는 대조군에 비해 치조능 부위에서 미성숙 골의 형성을 보여주었다. 주위 치조골에서는 편측으로 활발한 골조직의 형성이 관찰되었다. 치수에서는 치근단 부위에서 많은 혈관의 파열을 보여 주었고 공포의 형성과 충혈을 나타냈다. 2. 힘을 제거 된 후3주간의 보정을 한 군의 모든 치주인대의 주행 방향과 간격이 대조군과 같이 회복되었으며 밀도는 치밀하게 나타났다. MT소견에서 1군에서 형성된 치조능의 미성숙 골이 석회화가 이루어짐을 보여주었고 치수의 공포와 충혈의 감소를 보여주었다. 수복과정에서의 치수의 섬유화가 더욱 진행되었다. 이상의 결과를 종합해 볼 때, 3주간의 회복 기간 중에도 치주 인대 부위에서는 대조군과 유사한 조직 소견을 보여 상당한 회복능력을 보였지만 치수에서는 섬유화가 진행됨을 보여 더욱 긴 회복 기간이 필요함을 보여주었다.

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랫드에서의 생분해성 매선요법에 대한 조직학적 분석 (Histological Evaluation of Bioresorbable Threads in Rats)

  • 이창근;정재윤;황삼노;박찬오;황순재;조민정;신민희;김현호;이기종
    • 대한임상검사과학회지
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    • 제50권3호
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    • pp.217-224
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    • 2018
  • 매선요법은 다른 시술에 비해 비침습적이고, 인체 내에서 생분해가 일어나는 물질로 제작되기 때문에 성형수술에서 각광받고 있다. 상업적으로 이용가능한 매선의 종류로는 poly-L-lactic acid (PLLA), polycarprolactone (PCL), polydioxanone (PDO) 등이 있으나, 매선으로 사용되는 생분해성 물질들의 종류에 따른 차이점이나, 콜라겐을 형성하는데 있어서 매선의 어떤 부분이 결정적으로 작용하는지에 대한 연구는 잘 알려져 있지 않다. 따라서 이 연구에서는 현재 판매되고 있는 3가지 생분해성 물질(PLLA, PCL, PDO)로 만들어진 매선을 동물모델에서 그 효과를 비교하고, 매선의 콜라겐생성능에 미치는 영향 인자가 무엇인지를 분석하였다. 매선을 랫드의 등에 삽입하여 2주, 4주, 8주, 12주동안 경과를 관찰하고 특수염색을 통해 콜라겐의 생성 정도를 비교하였다. 매선에 의한 조직학적 변화를 관찰하기 위해서, 조직검체는 H&E, Masson's trichrome, Herovici's collagen stain이 진행되었다. 그 결과 3가지 모두 다 동일한 시기에 비슷한 양의 신생콜라겐을 형성하는 것을 관찰할 수 있었고, 3가지 매선 모두 type 3의 콜라겐을 형성하는 것을 확인할 수 있었다. 또한 생성되는 콜라겐의 양은 매선이 피부조직에 닿는 면적이 크면 클수록 증가하는 것을 관찰할 수 있어 한 번의 시술만으로도 많은 양의 매선이 들어갈 수 있는 PDO cavern타입의 매선이 가장 많은 콜라겐을 형성하는 것을 확인할 수 있었다.

Morphology and histology of the olfactory organ of two African lungfishes, Protopterus amphibius and P. dolloi (Lepidosirenidae, Dipnoi)

  • Hyun Tae Kim;Jong Young Park
    • Applied Microscopy
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    • 제51권
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    • pp.5.1-5.7
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    • 2021
  • The olfactory organs of two African lungfishes, Protopterus amphibius and P. dolloi, were investigated using a stereo microscope and a compound light microscope and were described anatomically, histologically, and histochemically. Like other lungfishes, these species present the following general features: i) elongated olfactory chamber (OC), ii) anterior nostril at the ventral tip of the upper lip, iii) posterior nostril on the palate of the oral cavity, iv) lamellae with multiple cell types such as olfactory receptor neurons, supporting cells, basal cells, lymphatic cells, and mucous cells (MC), and vi) vomero-like epithelial crypt (VEC) made of glandular epithelium (GE) and crypt sensory epithelium. Some of these features exhibit differences between species: MCs are abundant in both the lamellar and inner walls of the OC in P. amphibius but occur only in lamellae in P. dolloi. On the other hand, some between feature differences are consistent across species: the GE of both P. amphibius and P. dolloi is strongly positive for Alcian blue (pH 2.5)-periodic acid Schiff (deep violet coloration), and positive with hematoxylin and eosin and with Masson's trichrome (reddish-brown staining), unlike the MCs of the two species which stain dark red with both Alcian blue (pH 2.5)-periodic acid Schiff and Masson's trichrome but respond faintly to hematoxylin and eosin. The differing abundance of MCs in the two lungfishes might reflect different degrees in aerial exposure of the olfactory organ, while the neutral and acid mucopolysaccharide-containing VEC, as indicated by staining properties of the MCs, is evolutionary evidence that P. amphibius and P. dolloi are the closest living relatives to tetrapods, at least in the order Dipnoi.

경락의 관점에서 본 근막 분리조직의 조직학적 연구 (Histological Examination of Tissue Isolated from Fascia with a View of Meridian System)

  • 김동희;등영건;장병수;정한석;김단;권기록;이규재
    • Applied Microscopy
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    • 제36권3호
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    • pp.183-194
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    • 2006
  • 동양의학 이론의 주요 관심사인 경락의 연구에 있어 생체 내 신호전달 체계와 해부학적 실체에 대한 단서를 확인하기 위하여 본 실험을 수행하였다. 1960년대 이루어졌던 봉한학설의 이론과 문헌을 근거로 하여 토끼의 복막에 존재하는 봉한관으로 생각되는 반투명의 회백색의 탄성이 있는 섬유상 구조물을 분리하여 조직학적으로 관찰하였다. 각 조직 샘플은 봉한관 확인을 위하여 hematoxylin eosin과 Masson's trichrome 염색, 교원섬유는 Van Gieson's 염색, 신경은 Kluver Barrera's luxol fast blul 염색을 하여 비교함으로써 구성성분에 대한 분석을 실시하였다. 광학현미경상에서 관찰된 조직들은 교원섬유와 신경으로 구성된 집합체였으며 다발의 형태를 이루고 있었고, 여러 개의 myoid spindle형태의 세포들이 종으로 배열되어 있었다. 이러한 세포들은 신경의 슈반세포이며 봉한관의 myoid형태의 세포들과 같은 소견이었다. 같은 부위를 전자현미경으로 관찰한 결과 잘 배열된 교원섬유와 신경을 관찰할 수 있었으며 신경다발 사이로 교원섬유가 둘러싸고 있었다. 이상의 결과를 종합해 보면 복막에 존재하는 봉한관은 교원섬유와 어우러진 신경임을 확인할 수 있었고 신경과 어우러져 존재하는 교원섬유의 절연성과, 침의 치료에 있어 교원섬유가 주위의 신경과의 신호전달 체계에 영향을 미치는 점을 고려하여 볼 때 교원섬유와 신경 그리고 혈관의 유기적인 관계 속에서 교원섬유의 새로운 역할 규명에 대한 연구가 필요할 것으로 사료된다.

실험적으로 유도된 당뇨백서의 심근병증에 관한 조직병리학적 연구 (HISTOPATHOLOGICAL STUDY ON CARDIOMYOPATHY IN EXPERIMENTALLY INDUCED DIABETIC RATS)

  • 안진수;이재훈
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제18권3호
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    • pp.488-499
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    • 1996
  • Diabetes mellitus revealed a chronic disorder of lipid, carbohydrate and protein metabolism characterized by insulin deficiency, and a striking tendency toward development of atherosclerosis, microangiopathy, nephropathy, neuropathy and recently cardiomyopathy etc. The mechanism of heart failure in patients with diabetic cardiomyopathy is not clear but diabetic cardiomyopathy usually occurs in persons with long standing diabetes. After diabetes induced in made Sprague- Dawley strain rats by injection of streptozotocin(60mg/kg), cardiac tissue with hematoxylin-eosin and Masson's trichrome stain was examined at 3 days, 1, 2, 4, 6 weeks later under light microscope. The results were obtained as follows : 1. In H&E stain of control group, myocardiac cells were shorter than skeletal muscle cell, which was branched out and connected each other at terminal with striation, intercalated disk and nucleus at center of cell. 2. In MT stain of control group, a few of collagen fibrile were seen at periva scular interstium, but wasn't seen between skeletal muscle fiber, and cardiac muscle was seen in various size. 3. In MT stain of experimental group, increased collagen fiber deposition at perivascular interstiums were seen periodically. 4. In MT stain of experimental group, increased collagen fiber deposition at interstitial matrix between perimyocardiac cells were seen at 3 day, 4 weeks and 6 weeks after DM induction. 5. In H&E stain of experimental group, partial degeneration of myocardiac cells was seen after 4 weeks of DM induction. From above results, streptozotocin induced diabetes mellitus increased collagen around perivascular and between intercellular matrix in heart.

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마늘 추출물이 간 손상 랫드의 조직학적 변화에 미치는 효과 연구 (A Study of the Histological Effects of Garlic Extract in a Liver-damage Rat Model)

  • 이태종;민경진
    • 한국환경보건학회지
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    • 제36권4호
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    • pp.279-287
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    • 2010
  • In this study we examined the effects of water extract of garlic on carbon tetrachloride-induced liver injury, and demonstrated increased beneficial enzyme and anti-oxidant activity as well as histopathological changes (by Hematoxylin-Eosin (H&E) staining, Trichrome staining, and TEM examination), and showed that the treatment was dose-dependent and safe. A total of 42 male Sprague-Dawley rats were divided equally (n=7) into six groups. To induce hepatotoxicity in these subjects, carbon tetrachloride diluted in an equal volume of olive oil was intraperitoneally administrated at 0.5 ml/kg (0.20 g/kg/day) once a day for five days. Water extract of Korean-grown garlic was administered via a stomach sonde once a day, 5 days a week, for a total of 4 weeks. Groups received 0.35 g/kg (E1), 0.70 g/kg (E2), or 1.40 g/kg (E3), with the dose adjusted for body weight. Administration of garlic extract resulted in positive physiological effects in terms of reduced oxidative stress and toxicity, and induced functional changes in the liver. Comparing the subject groups (E1, E2, E3) administered different doses of garlic extract, the importance of morphological analysis in further studies is emphasized, because morphological changes indicating hepatotoxicity could occur, even though beneficial enzyme activities were found to be elevated.