• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.4
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• pp.694-699
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• 2017

In accordance with the development of information and communications technology, a network user has to be able to use quality of service (QoS)-based multimedia services easily. Thus, information and communications operators began to focus on a technique for providing multimedia services. The IP Multimedia Subsystem (IMS) is a platform based on Internet Protocol (IP) as a technology for providing multimedia services and application services. The emerging 5G networks are described as having massive capacity and connectivity, adaptability, seamless heterogeneity, and great flexibility. The explosive growth in network services and devices for 5G will cause excessive traffic loads. In this paper, software-defined networking (SDN) is applied as a kind of virtualization technology for the network in order to minimize the traffic load, and Simple Network Management Protocol (SNMP) is used to provide more efficient network management. To accomplish these purposes, we suggest the design of a dynamic routing algorithm to be utilized in the IMS network using SDN and an SNMP private management information base (MIB). The proposal in this paper gives information and communications operators the ability to supply more efficient network resources.

• Applied Microscopy
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• v.35 no.1
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• pp.31-39
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• 2005

Synapses are contact points where one neuron communicates with another. The morphological change of synapses under various physiological or pathological conditions has long been hypothesized to modify their functional properties. 3-dimensional (3-D) reconstruction of synapses with serial ultrathin sections has contributed to the understanding of ultrastructural dimensions and compositions of synapses. The 3-D reconstruction procedures, however, require a great amount of expertise as well as include prohibitively timeconsuming processes. Here, we introduce efficient 3-D reconstruction technique using high-voltage electron microscopy (HVEM). Primarily, we established an optimal section thickness and staining condition to observe synaptic structures in detail under HVEM. The result showed that synaptic profiles were preserved at the section thickness of 250 nm without the overlapping of synaptic ultrastructures. An increase in the reaction time of en bloc staining was most efficient to enhance contrast than the extension of postembedding staining or the addition of uranyl acetate during dehydration. Then, 3-D reconstruction of parallel fiber-Purkinje cell synapses in the rat cerebellum was carried out with serial HVEM images and reconstruction software. The images were aligned and the contours of synapses were outlined on each section. 3-D synapses were finally extracted from the section files by grouping all the synaptic contours. The reconstructed synapse model clearly demonstrated the configuration of pre and postsynaptic components. These results suggest that 3-D reconstruction of synapses using HVEM is much efficient and suitable for massive quantitative studies on synaptic connectivity than conventional TEM approach using numerous ultrathin sections.