• The Bulletin of The Korean Astronomical Society
• /
• v.41 no.1
• /
• pp.76.2-76.2
• /
• 2016

We present near-infrared (0.8 - 1.8 microns) spectra of 63 bright (J_mag < 10) stars observed with Low Resolution Spectrometer (LRS) onboard the rocket-borne Cosmic Infrared Background Experiment (CIBER). Two Micron All Sky Survey (2MASS) photometry information is used to find cross-matched stars after reduction and extraction of the spectra. We identify the spectral types of observed stars by comparing with spectral templates from the Infrared Telescope Facility (IRTF) library. All the observed spectra are consistent with late F to M stellar spectral types, and we identify various infrared absorption lines. As our observations are performed above the Earth's atmosphere, our spectra are free from telluric contamination. Including HST/NICMOS and Cassini/VIMS, the spectral coverage has rarely been achieved in space, and the methods developed here can inform statistical studies with future low-resolution spectral measurements such as GAIA photometric and radial velocity spectrometer.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2016.02a
• /
• pp.384.1-384.1
• /
• 2016

We reported that amorphous silicon (a-Si) thin film provide sample plate exhibiting a multimodality to measure biomolecules by secondary ion mass spectrometry (SIMS) and laser desorption/ionization mass spectrometry (LDI-MS). Kim et al.1 reported that a-Si thin film were suitable to detect small molecules such as drugs and peptides by SIMS and LDI-MS. Recently, bacterial identification has been required in many fields such as food analysis, veterinary science, ecology, agriculture, and so on.2 Mass spectrometry is emerging for identifying and profiling microbiology samples from its advantageous characters of label-free and shot-time analysis. Five species of bacteria - S. aureus, G. glutamicum, B. kurstaki, B. sphaericus, and B. licheniformis - were sampled for MS analysis without lipid extraction in sample preparation steps. The samples were loaded onto the a-Si thin film with a thickness of 100 nm which did not only considered laser-beam penetration but also surface homogeneity. Mass spectra were recorded in both positive and negative ionization modes for more analytical information. High reproducibility and sensitivity of mass spectra were demonstrated in a mass range up to mass-to-charge ratio(m/z) 1200 by applying the a-Si thin film in mentioned above MS. Principle component analysis (PCA) - a popular statistical analysis widely used in data processing was employed to differentiate between five bacterial species. The PCA results verified that each bacterial species were readily distinguished and differentiated effectively from our MS approach. It shows a new opportunity to rapid bacterial profiling and identification in clinical microbiology. More details will be discussed in the presentation.

• Plant Biotechnology Reports
• /
• v.4 no.3
• /
• pp.193-199
• /
• 2010

An olive-green mutant was generated in Synechocystis sp. strain PCC 6803 by inactivation of the sll0396 gene. Whole-cell absorption spectra of the mutant revealed the missing of phycocyanin peak. An investigation of the low-temperature fluorescence emission spectra revealed that the $sll0396{\Omega}$ mutant has a reduced amount of phycocyanin. Western blot analysis showed that the mutant contained less phycocyanin ${\beta}$- and ${\alpha}$-subunits and lacked the 30- and 32-kDa linker polypeptides, and northern blot analysis revealed that the transcription of the 1.4-kb cpcBA gene encoding the phycocyanin ${\beta}$- and ${\alpha}$-subunits was lower in the mutant. The Sll0396 protein has a DNA-binding motif and shares homology with known response regulators. Our results indicate that Sll0396 plays a regulatory role in the transcription of the phycocyanin genes during phycobilisome synthesis.

• Bulletin of the Korean Chemical Society
• /
• v.32 no.3
• /
• pp.915-920
• /
• 2011

Four esters of succinic and maleic acid were synthesized, separated by thin-layer chromatography (TLC), and identified using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). A comparison of matrix materials showed that 2,6-dihydroxybenzoic acid (2,6-DHB) yielded a greater ionization efficiency than 2,5-DHB prior to TLC separation. The location of each ester sample on the TLC plate was estimated by comparing the developed plate with a duplicate plate that had been visualized by immersion in a $KMnO_4$ solution. Generally, mass spectra obtained from the $KMnO_4$-visualized plate were relatively poor. Reproducible mass spectra with high peak abundance were difficult to obtain using the 2,6-DHB matrix from crude synthetic esters extracted from the TLC plates. Significant improvements in both reproducibility and sensitivity were realized by using pencil lead as the MALDI matrix. The current methodology will be beneficial to organic chemists since it can provide a guideline for simple and rapid characterization of small organic compounds.

• Preventive Nutrition and Food Science
• /
• v.3 no.2
• /
• pp.111-118
• /
• 1998

To develop a simple, rapid and simultaneous analytical method of phenolic compounds using gas chromatography (GC) and gas chromatography/mass spectrophometer (GC/Ms), this experiment was carried out to search the retention times of capillary columns and the characteristics of fragment ions in electron impact mass spectra. Most of trimethylsilyl derivatives and underivatized phenolic compounds were separated very well on three kinds of capillary columns(HP-1), Ultra-2 and HP-35). Quantitiative determination of phenolic compounds was achieved by internal standards (p-hydroxybenzoic acid iopropyl ester, p-hydroxybenzoic acid ethyl ester). Calibration plts were linear in the investigated range, and the limits of detection were about 5 ng at split mode method. When analyzed by three columns, theseparation times were fairly constant on two nonpolar columns, but a few compounds showed slightly different separation order by the itnermediate polar HP-35 column. The important characteristic patterns of TMS derivatives of phenolic compounds on the EI/MS spectrra appeared at the base peak of [M-15]+ ion and presented at high abundance in most TMS derivatives of phenoloc compounds. [M]+, [M-CH3-COO]+, [M-Si(CH3)4]+ and [M-Si(CH3)4 -CH3]+ also observed in mass spectra of these compounds . Although several compounds have the same retention times on GC column, it might be possible to identify these compounds by the different patternsof mass frgement ions. The TMS derivatives, thus , provide additional information for identification of phenolic compounds in biological systems.

• Mass Spectrometry Letters
• /
• v.11 no.4
• /
• pp.77-81
• /
• 2020

Characterization of the various chemical aspects of composite polymers is important for quality control of manufactured polymers. In this study, we compared three suitable matrices (α cyano-4-hydroxycinnamic acid [CHCA], 2,5 dihydroxy benzoic acid [2,5-DHB], and dithranol), to characterize various synthetic polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Although the spectra obtained with the CHCA and 2,5-DHB matrices were generally good, in certain samples ghost peaks disappeared only when dithranol was used as the matrix. Furthermore, we examined the use of sodium trifluoroacetate (NaTFA) as an additive to reduce interference by metals and copolymers in the spectra. In conclusion, appropriate selection of a matrix, according to the characteristics of the polymer, and the use of additives to improve sensitivity are important considerations for polymer analysis and development.

• The Bulletin of The Korean Astronomical Society
• /
• v.36 no.1
• /
• pp.52.2-52.2
• /
• 2011

The correlation between black hole mass and galaxy stellar velocity dispersion gives an important clue on the black hole growth and galaxy evolution. In the case of AGN, however, it is extremely difficult to measure stellar velocity dispersions in the optical spectra since AGN continuum dilutes stellar absorption features. In contrast, stellar velocity dispersions of active galaxies can be measured in the near-IR, where AGN-to-star flux ratio is much smaller, particularly with the laser-guide-star adaptive optics. However, it is crucial to test whether the stellar velocity dispersion measured from the near-IR spectra is consistent with that measured from the optical spectra. Using the TripleSpec at the Palomar 5-m Telescope, we obtained high quality spectra ranging from 1 to 2.4 micron for a sample of 35 nearby galaxies, for which dynamical black hole masses and optical stellar velocity dispersion measurements are available, in order to calibrate the stellar velocity dispersion in the near-IR. In this poster, we present the initial results based on 10 galaxies, with the stellar velocity dispersion measured in the H-band.

• The Bulletin of The Korean Astronomical Society
• /
• v.36 no.1
• /
• pp.49.1-49.1
• /
• 2011

Utilizing single-epoch spectra and the empirical relation between the size of the broad-line region and AGN continuum luminosity, the so-called single-epoch method has been widely used for estimating AGN black hole masses. However, the systematic uncertainties and the potential biases of this method are not well examined. Taking the full advantage of the high-quality homogeneous spectra from the Lick AGN Monitoring Project (LAMP), we investigate in detail the uncertainties of single-epoch mass estimates by comparing with the reverberation-mapping results. We find that the uncertainty due to AGN variability is less than 0.1 dex, while there is a systematic offset between single-epoch masses and reverberation masses. Particularly, narrow-line Seyfert 1 galaxies show that the Hbeta line widths measured from single-epoch (or mean) spectra are systematically larger than those from rms spectra, indicating a potential bias of single-epoch masses. We will present the detailed measurement method, the test of virial assumption, and the systematic uncertainties.

• The Bulletin of The Korean Astronomical Society
• /
• v.36 no.1
• /
• pp.48.2-48.2
• /
• 2011

Using the newly commissioned Fiber-Multi-Object-Spectrograph at the Subaru telescope, we obtained near-IR spectra of a sample of 19 AGNs at 0.6 < z < 2.6, selected from the NOAO Deep Wide-Field Survey (NDWFS) Bootes field, in order to calibrate high-z black hole mass (MBH) estimators. MBHs are generally determined through the kinematics of ionized gas clouds around the black hole assuming virial equilibrium. The velocity profiles of $H{\beta}/H{\alpha}$, MgII and CIV are used to infer the gas kinematics of low-z, mid-z, and high-z quasars, respectively. However, the MBH based on MgII and CIV is not very well calibrated. We compare the $H{\alpha}$ - based MBH estimates from the new FMOS near-IR spectra, with the MgII-based MBH estimates from our existing optical spectra, and investigate the systematic differences.

• Earthquakes and Structures
• /
• v.26 no.3
• /
• pp.219-228
• /
• 2024

This study examines damping modification factors (DMFs) of elastic input energy spectra corresponding to a set of 116 earthquake ground motions. Mean input energy per mass spectra and mean DMFs are presented for both considered ground motion components. Damping ratios of 3%, 5%, 10%, 20%, and 30% are used and the 5% damping ratio is considered the benchmark for DMF computations. The geometric mean DMFs of the two horizontal components of each ground motion are computed and coefficients of variation are presented graphically. The results show that the input energy spectra-based DMFs exhibit a dependence on the damping ratio at very short periods and they tend to be nearly constant for larger periods. In addition, mean DMF variation is obtained graphically for also the damping ratio, and mathematical functions are fitted as a result of statistical analyses. A strong correlation between the computed DMFs and the ones from predicted equations is observed.