• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.5789-5795
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• 2013

Background: The high mobility group box 1 (HMGB1) protein is a widespread nuclear protein present in most cell types. It typically locates in the nucleus and functions as a nuclear cofactor in transcription regulation. However, HMGB1 can also localize in the cytoplasm and be released into extracellular matrix, where it plays critical roles in carcinogenesis and inflammation. However, it remains elusive whether HMGB1 is relocated to cytoplasm in clear cell renal cell carcinoma (ccRCC). Methods: Nuclear and cytoplasmic proteins were extracted by different protocols from 20 ccRCC samples and corresponding adjacent renal tissues. Western blotting and immunohistochemistry were used to identify the expression of HMGB1 in ccRCC. To elucidate the potential mechanism of HMGB1 cytoplasmic translocation, HMGB1 proteins were enriched by immunoprecipitation and analyzed by mass spectrometry (MS). Results: The HMGB1 protein was overexpressed and partially localized in cytoplasm in ccRCC samples (12/20, 60%, p<0.05). Immunohistochemistry results indicated that ccRCC of high nuclear grade possess more HMGB1 relocation than those with low grade (p<0.05). Methylation of HMGB1 at lysine 112 in ccRCC was detected by MS. Bioinformatics analysis showed that post-translational modification might affect the binding ability to DNA and mediate its translocation. Conclusion: Relocation of HMGB1 to cytoplasm was confirmed in ccRCC. Methylation of HMGB1 at lysine 112 might the redistribution of this cofactor protein.

• 화약ㆍ발파
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• 제27권1호
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• pp.21-31
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• 2009

터널의 굴착이나 응력 재분포에 의해 터널 주변에는 암반 손상대(EDZ) 구간이 발생하게 되며, 이는 암반의 역학적 수리적 물성변화를 초래하여 추가적인 균열발생 및 지하수의 주요 공급로 역할을 하게 된다. 본 연구에서는 방사성폐기물 처분연구시설(KURT)을 대상으로 현장실험을 통해 암반변형계수를 측정하였고, 이를 바탕으로 손상대 영역을 세분화하여 FLAC2D를 이용한 전산해석을 실시하였다. 실험결과 KURT 주변에는 측정지점에 따라 0.6~1.8m의 손상영역이 발생하였으며, 손상대 구간의 변형계수 값은 주변 암반 변형계수의 약 40%에 해당하였다. 암반손상대로 인해 KURT 내 터널변위는 약 65%의 추가변위가 발생하였고, 최대 주응력은 58% 감소되었음을 확인하였다. 또한 손상대 영역의 고려로 인해 터널의 좌 우측 하단부에 발생되었던 소성 영역이 터널의 천정 및 바닥부근으로 확대되는 경향을 보였다.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제19권2호
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• pp.197-215
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• 2015

Modeling water flow in variably saturated, porous media is important in many branches of science and engineering. Highly nonlinear relationships between water content and hydraulic conductivity and soil-water pressure result in very steep wetting fronts causing numerical problems. These include poor efficiency when modeling water infiltration into very dry porous media, and numerical oscillation near a steep wetting front. A one-dimensional finite element formulation is developed for the numerical simulation of variably saturated flow systems. First order backward Euler implicit and second order Crank-Nicolson time discretization schemes are adopted as a solution strategy in this formulation based on Picard and Newton iterative techniques. Five examples are used to investigate the numerical performance of two approaches and the different factors are highlighted that can affect their convergence and efficiency. The first test case deals with sharp moisture front that infiltrates into the soil column. It shows the capability of providing a mass-conservative behavior. Saturated conditions are not developed in the second test case. Involving of dry initial condition and steep wetting front are the main numerical complexity of the third test example. Fourth test case is a rapid infiltration of water from the surface, followed by a period of redistribution of the water due to the dynamic boundary condition. The last one-dimensional test case involves flow into a layered soil with variable initial conditions. The numerical results indicate that the Crank-Nicolson scheme is inefficient compared to fully implicit backward Euler scheme for the layered soil problem but offers same accuracy for the other homogeneous soil cases.

• 지질공학
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• 제8권3호
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• pp.225-234
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• 1998

지하공동의 거동은 굴착전에 암반에 존재하는 초기응력의 크기와 굴착에 따른 공동주변에서의 응력재분배에 많은 영향을 받는다. 암반과 같은 탄소성 재료인 경우 굴착으로 인한 공동주변의 거동은 응력경로의 영향을 받기 때문에 굴착방법과 순서 등에 따라 해석결과는 달라지게 된다. 따라서 지하 원자력발전소, 방사성폐기물 영구처분시설, 원유비축시설 등과 같은 대단면을 갖는 지하공동을 보다 합리적으로 설계하기 위해서는 해석과정에 굴착으로 인한 영향을 반영하는 것이 바람직하다. 본 연구에서는 굴착으로 인한 응력변화를 파악하기 위해서 유한요소법과 개별요소법에 의한 수치해석을 각각 수행하였다. 해석결과를 분석한후 합리적인 굴착단면형상, 굴착방법 및 순서, 지보효과, 다공동 굴착시 인접공동의 굴착으로 인한 영향 등을 검토하였다.

• Wind and Structures
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• 제28권1호
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• pp.31-47
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• 2019

The accurate prediction of snow distributions under the wind action on roofs plays an important role in designing structures in civil engineering in regions with heavy snowfall. Affected by some factors such as building shapes, sizes and layouts, the snow drifting on roofs shows more three-dimensional characteristics. Thus, the research on three-dimensional snow distribution is needed. Firstly, four groups of stepped flat roofs are designed, of which the width-height ratio is 3, 4, 5 and 6. Silica sand with average radius of 0.1 mm is used to model the snow particles and then the wind tunnel test of snow drifting on stepped flat roofs is carried out. 3D scanning is used to obtain the snow distribution after the test is finished and the mean mass transport rate is calculated. Next, the wind velocity and duration is determined for numerical simulations based on similarity criteria. The adaptive-mesh method based on radial basis function (RBF) interpolation is used to simulate the dynamic change of snow phase boundary on lower roofs and then a time-marching analysis of steady snow drifting is conducted. The overall trend of numerical results are generally consistent with the wind tunnel tests and field measurements, which validate the accuracy of the numerical simulation. The combination between the wind tunnel test and CFD simulation for three-dimensional typical roofs can provide certain reference to the prediction of the distribution of snow loads on typical roofs.

• 운동영양학회지
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• 제16권2호
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• pp.65-71
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• 2012

Oxygen is the final acceptor of electron transport from fat and carbohydrate oxidation, which is the rate-limiting factor for cellular ATP production. Under altitude hypoxia condition, energy reliance on anaerobic glycolysis increases to compensate for the shortfall caused by reduced fatty acid oxidation [1]. Therefore, training at altitude is expected to strongly influence the human metabolic system, and has the potential to be designed as a non-pharmacological or recreational intervention regimen for correcting diabetes or related metabolic problems. However, most people cannot accommodate high altitude exposure above 4500 M due to acute mountain sickness (AMS) and insulin resistance corresponding to a increased levels of the stress hormones cortisol and catecholamine [2]. Thus, less stringent conditions were evaluated to determine whether glucose tolerance and insulin sensitivity could be improved by moderate altitude exposure (below 4000 M). In 2003, we and another group in Austria reported that short-term moderate altitude exposure plus endurance-related physical activity significantly improves glucose tolerance (not fasting glucose) in humans [3,4], which is associated with the improvement in the whole-body insulin sensitivity [5]. With daily hiking at an altitude of approximately 4000 M, glucose tolerance can still be improved but fasting glucose was slightly elevated. Individuals vary widely in their response to altitude challenge. In particular, the improvement in glucose tolerance and insulin sensitivity by prolonged altitude hiking activity is not apparent in those individuals with low baseline DHEA-S concentration [6]. In addition, hematopoietic adaptation against altitude hypoxia can also be impaired in individuals with low DHEA-S. In short-lived mammals like rodents, the DHEA-S level is barely detectable since their adrenal cortex does not appear to produce this steroid [7]. In this model, exercise training recovery under prolonged hypoxia exposure (14-15% oxygen, 8 h per day for 6 weeks) can still improve insulin sensitivity, secondary to an effective suppression of adiposity [8]. Genetically obese rats exhibit hyperinsulinemia (sign of insulin resistance) with up-regulated baseline levels of AMP-activated protein kinase and AS160 phosphorylation in skeletal muscle compared to lean rats. After prolonged hypoxia training, this abnormality can be reversed concomitant with an approximately 50% increase in GLUT4 protein expression. Additionally, prolonged moderate hypoxia training results in decreased diffusion distance of muscle fiber (reduced cross-sectional area) without affecting muscle weight. In humans, moderate hypoxia increases postprandial blood distribution towards skeletal muscle during a training recovery. This physiological response plays a role in the redistribution of fuel storage among important energy storage sites and may explain its potent effect on changing body composition. Conclusion: Prolonged moderate altitude hypoxia (rangingfrom 1700 to 2400 M), but not acute high attitude hypoxia (above 4000 M), can effectively improve insulin sensitivity and glucose tolerance for humans and antagonizes the obese phenotype in animals with a genetic defect. In humans, the magnitude of the improvementvaries widely and correlates with baseline plasma DHEA-S levels. Compared to training at sea-level, training at altitude effectively decreases fat mass in parallel with increased muscle mass. This change may be associated with increased perfusion of insulin and fuel towards skeletal muscle that favors muscle competing postprandial fuel in circulation against adipose tissues.