• 미생물학회지
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• 제40권2호
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• pp.160-166
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• 2004

The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$PO$_4$, 0.05% The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % $(KH_2PO_4$, 0.05% $MgSO_4{\cdot}7H_2O$, 0.2% L-asparagine, pH 4.5, and the optimal inoculum size and shaking speed were $1.5{\times}10^6$ spores/50 m1 medium and 150 rpm, respectively. On optimal conditions, 4.1 g/l of the cell mass was obtained at 28$^{\circ}C$ for 3 days. The mycelium were inoculated on 500 g of steamed rice using vinyl bag ($30.6{\times}44$ cm) and incubated at $30^{\circ}C$, 85% humidity for 21 days. Lactone form monacolin K was rapidly increased for 2 days and reached highest concentration of monacolin K (2,930 mg/kg) for 15 days, and monacolin K was decreased after 15 days.

• Ocean and Polar Research
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• 제45권4호
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• pp.201-209
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• 2023

Melosira nummuloides (KNU-HAPCC-101), which is a biological resource for fucoxanthin extraction, was separated from single cells, and optimal growth conditions were derived according to temperature, salinity, light intensity, light emitting diode, N:P ratio of culture medium, and dilution ratio of culture medium. These parameters were assessed to ascertain the best culture conditions for the most economical and efficient indoor mass production. The specific growth rate was the highest at 25℃ in temperature, but there was no significant difference between 15℃ and 20℃. M. nummuloides died at 0 psu in salinity, and the specific growth rate was the highest at 30 psu in respect to salinity. The light intensity was similar in all experimental groups except for 5 µmol photons m^-2 s^-2. The maximum biomass and specific growth rate in the light-emitting diode experiment were revealed at the red wavelength, but the concentration of chlorophyll-a was the lowest at the red wavelength whereas the white wavelength produced the highest chlorophyll-a concentration. In the experiment according to the ratio of nitrogen and phosphorus (N:P) based on the f/2 culture medium, the growth rate was significantly higher in the 15:1 to 50:1 ratio range. The growth rate according to the dilution concentration of the f/2 culture medium was the highest in the f/2 culture medium, but did not show a significant difference from the f/4 culture medium. M. nummuloides can be mass-cultured under conditions of a temperature of 15-25℃, a salinity of 15-35 psu, light intensity of 25-150 µmol photons m^-2 s^-2, and f/4 media concentration level with an N:P ratio 15:1 or more. Based on the data of this study, it is expected that fucoxanthin can be produced in a highly efficient manner through the development of a mass culture system.

• 한국환경과학회지
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• 제8권5호
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• pp.575-586
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• 1999

From the environmental aspects, primary productivity of phytoplankton plays the most improtant role in enhancement of marine culture oyster production. This study may be divided into two branches; one is estimation of maximum oyster meat production per unit facility(Carrying Capacity) under the present enviromental conditions in Kamak Bay, the other is improvement of carrying from increase of primary productivity by changing the environmental conditions that cause not ot form an unfavorable environment such as the formation of oxygen deficient water mass using the eco-hydrodynamic model. By simulation of three-dimensional hydrdynamic model and ecosystem model, the comparison between observed and computed data showed good agreement. The results of sensitivity analysis showed that phytoplankton maximum growth rate was the most important parameter for phytoplankton and dissolved oxygen. The estimation of mean primary productivity of Wonpo, Kamak, Pyongsa, and Kunnae culture grounds in Kamak Bay during culturing period were 3.73gC/$m^2$/d, 2.12gC/$m^2$/d, 1.98gC/$m^2$/d, and 1.26gC/$m^2$/d, respectively. Under condition not ot form the oxygen deficient water mass, four times increasing of pollutants loading as much as the present loading from river increased mean primary productivity of whole culture grounds to 4.02gC/$m^2$/d. Sediment N, P fluxes that allowed for 35% increasing from the present conditions increased mean primary productivity of whole culture grounds to 3.65gC/$m^2$/d. Finally, ten times increasing of boundary loadings from the present conditions increased mean primary productivity of whole culture grounds to 3.95gC/$m^2$/d. The maximum oyster meat production per year and that of unit facility in actual oyster culture grounds under the present conditions were 6,929ton and 0.93ton, respectively. This 0.93ton/unit facility is considered to be the carrying capacity in study area, and if the primary productivity is increased by changing the environmental conditions, oyster production can be increased.

• 한국수산과학회지
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• 제43권6호
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• pp.687-692
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• 2010

The mass cultivation of Ecklonia cava Kjellman was studied as a potential biomass source for the extract industry in Korea. Experiments were conducted to investigate the optimal conditions for artificial seed production and mass cultivation of this species. Maximum growth and young thalli development in the nursery culture area occurred at 2 m depth, whereas maximum growth of thalli in the main culture area occurred at 1 m depth. Production of E. cava was between 2.6 and 3.6 kg wet wt. $m^{-1}$ after depth control and removal of fouling organism, etc. The relationship between optimal water depth for culture and underwater irradiance during the E. cava cultivation was calculated as: y = -0.718x + 8.042 ($r^2$=0.976). The growth rates achieved in this trial indicate that E. cava cultures could produce and supply sufficient biomass.

• 한국컴퓨터정보학회논문지
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• 제10권5호
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• pp.301-312
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• 2005

본 논문에서는 문화산업이 국가경쟁력의 새로운 척도로 부각되는 가운데 국가단위에서 지역도시단위로 옮아가고 있고 지역 특성화 방안으로 문화산업 즉, 엔터테인먼트산업에 대한 지역 및 주민의 요구가 크고 다양하게 증대되고 있다. 문화산업의 도시별 차이점을 고찰함으로서 대 . 중소도시별 상관관계와 문화산업의 접근방법에 따른 이해와 이용의 차이를 분석하였고 대도시와 중소도시에 따른 변화나 문화정책도 연구목적으로 하였다. 연구결과에서 첫째, 문화가 변화함에 따라 문화산업이 변화하는 것으로 나타났다. 둘째, 사회적 여건에 따라 도시별 차이점을 발견할 수 있었는데 대도시에선 대중문화 부상이 영향을 미치는 요인으로 나타났으며 중소도시에서는 대중문화 부상이 영향을 미치고 있으나 문화생활화도 중요시됨을 도출 할 수 있었다. 셋째, 경제적인 여건에 따라 도시별 차이점을 발견할 수 있었는데 대도시에선 문화유통체계다양화와 문화상품화, 문화클러스터화가 고루 영향을 미치는 요인으로 나타났으며 중소도시에서는 문화유통체계다양화와 문화상품화가 영향을 미치고 있으나 문화 클러스터화에 대해 영향을 못 미치는 것으로 나타났다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.312-315
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• 2005

A process for efficient recycle fed-batch culture was carried out to increase cell mass and spore production by Lactic acid bacteria isolated from Kimchi. A large quantity of cell mass obtained by feeding concentration of sugar in recycle fed-batch culture. When the high density of salt was created that the cell mass was come-down. In this study, cultured in different feeding concentration of sugar conditions. Lactic acid bacteria by recycle fed-batch culture was investigated in 2L working volume of fermenter, obtained the maximum cell mass was 15.17g/L.

• 한국유기농업학회지
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• 제5권1호
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• pp.87-99
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• 1996

Cultural conditions for mass production of the antagonistic bacteria, Bacillus subtills CAP134 against pathogens causing major airborne diseases to apple tree, effect of temperature, pH, carbon and nitrogen source in the culture broth were investigated. The bacterial growth was most vigorous when the temperature and pH of the culture broth was 30~$35^{\circ}$C, and 7, respectively. As for carbon source, dextrose was best followed in order by dextrose(monosaccharides)>sucrose(disaccharides)$\geq$saccharose(di-saccharides)>starch (polysaccharides). Among different sugars, bacterial growth was favored by in the order of brown, black and white sugars, indicating that the bacterial growth might be promoted by the minor elements presented as impurities in the less purified sugars. As for nitrogen source, organic forms were better to bacterial growth than inorganic forms, that is polypeptone was best followed in order by soy sauce, soybeen milk and inoganic nitrogens. Differences in bacterial growth among different forms of inorganic nitrogen were negligible.

• 한국유기농업학회지
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• 제6권1호
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• pp.85-98
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• 1997

Cultural conditions for mass production of the antagonistic bacteria, Bacillus subtills CAP141 against pathogens causing major airborne diseases to apple trees, effects of temperature, pH, carbon and nitrogen source in the culture broth were investigated. The bacterial growth was most vigorous when the temperature and pH of the culture broth was 30~35$^{\circ}$C and 7, respectively. As for carbon source, dextrose was best followed in order by dextrose(monosaccharide) > sucrose(disaccharide) $\geq$ saccharose (market disaccharides) > starch (polysaccharieds). Among different sugars, bacterial growth was favored by in the order of brown, black and white supars, indicating that the bacterial growth might be promoted by the minor elements presented as impurities in the less purified sugars. As for nitrogen source, organic forms were better to bacterial growth than inorganic forms, that is polypeptone was best followed in order by soy sauce, soybeen milk and inorganic nitrogens. Differences in bacterial growth among different forms of inorganic nitrogen were negligible.

• 복식문화연구
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• 제11권5호
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• pp.647-656
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• 2003

Mass customization utilizes the new computer technologies, supply chain management, product information management, and some level of customization of the product for the individual customer. This study aimed at grasping the actual conditions of mass customization of domestic men's suit industry and the use of MTM CAD program. The MTM apparel production programs that are used by five leading men's suit manufacturers in Korea were compared. The results of the study showed that most of the manufacturers produced mass customized men's suits to supply extraordinarily large or small size suit based on the individual order. The information listed on an order sheet used at shops was not necessarily corresponding to the information in the MTM CAD program that is use for production of suit ordered from the shop. The pattern data base on the MTM CAD program are constructed based on the sizing system of individual manufacturer. The most manufacturers translated the customer's body dimensions into difference between the standard pattern and the customer's needed adjustment.

• Journal of Microbiology and Biotechnology
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• 제17권12호
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• pp.1996-2004
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• 2007

In this study, the optimization of culture medium using a Sterigmatomyces elviae mutant was investigated using statistical analysis to increase the cell mass and lactosucrose ($^4G-{\beta}$-D-galactosylsucrose) production. In basal medium, the cell mass and lactosucrose production were 4.12 g/l and 140.91 g/l, respectively. However, because of the low cell mass and lactosucrose production, optimization of culture medium was carried out to increase the cell mass and lactosucrose production. Culture media were optimized by the S. elviae mutant using analysis of variance (ANOVA) and response surface methodology (RSM). Central composite designs using RSM were utilized in this investigation. Quadratic models were obtained for cell mass and lactosucrose production. In the case of cell mass, optimal components of the medium were as follows: sucrose 1.13%, yeast extract 0.99%, bactopeptone 2.96%, and ammonium sulfate 0.40%. The predicted maximum value of cell mass was about 5.20 g/l and its experimental value was 5.08 g/l. In the case of lactosucrose production, optimal components of the medium were as follows: sucrose 0.96%, yeast extract 1.2%, bactopeptone 3.0%, and ammonium sulfate 0.48%. Then, the predicted maximum value of lactosucrose production was about 194.12 g/l and the corresponding experimental value was about 183.78 g/l. Therefore, by culturing using predicted conditions, the real cell mass and lactosucrose production increased to 23.3% and 30.42%, respectively.