• Title, Summary, Keyword: Mass culture

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Development of Solid Culture Media for the Mass Production of Beauveria bassiana Conidia (흰굳음병균(Beauveria bassiana) 포자의 대량배양을 위한 고체배지의 개발)

  • 서종복;진병래
    • Journal of Sericultural and Entomological Science
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    • v.38 no.1
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    • pp.31-35
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    • 1996
  • To develope a solid culture media for the mass culture of Beauveria bassiana conida, wheat bran was selected as C source and N source. The pellet media were prepared without(P-I) and with(P-II) solidified ingredient. The moisture for the growth of B. bassiana was required over 4 : 2 of Media : D.W (W/V). The growth of Beauveria bassiana on the media type was more effective in media of pellet type than that of powder type. In addition, production of Beauveria bassiana conidia on the media size was more effective in media of S type($\Phi$3mm) than that of L type($\phi$7mm). And the yield of Beauveria bassiana conidia at 2$0^{\circ}C$ cultivation on 3 weeks post inoculation was similar to that of $25^{\circ}C$. Above-mentioned results showed that pellet medium (P-I) was effective to growth of Beauveria, suggesting that the pellet media may be useful in the mass production of Beauveria bassiana conidia.

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A study on the effects of vertical mass irregularity on seismic performance of tunnel-form structural system

  • Mohsenian, Vahid;Nikkhoo, Ali
    • Advances in concrete construction
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    • v.7 no.3
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    • pp.131-141
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    • 2019
  • Irregular distribution of mass in elevation is regarded as a structural irregularity by which the modes with high energy levels are excited and in addition, it can lead the structure to withstanding concentration of nonlinear deformations and consequently, suffer from unpredictable local or global damages. Accordingly, with respect to the lack of knowledge and insight towards the performance of concrete buildings making use of tunnel-form structural system in seismic events, it is of utmost significance to assess seismic vulnerability of such structures involved in vertical mass irregularity. To resolve such a crucial drawback, this papers aims to seismically assess vulnerability of RC tunnel-form buildings considering effects of irregular mass distribution. The results indicate that modal responses are not affected by building's height and patterns of mass distribution in elevation. Moreover, there was no considerable effect observed on the performance levels under DBE and MCE hazard scenarios within different patterns of irregular mass distribution. In conclusion, it appears that necessarily of vertical regularity for tunnel-form buildings, is somehow drastic and conservative at least for the buildings and irregularity patterns studied herein.

Development of Liquid Culture Media for the Mass Production of Beauveria bassiana Blastospore (흰굳음병균(Beauveria basiana) 단균사의 대량배양생산을 위한 액체배지 개발)

  • 서종복;진병래
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.172-175
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    • 1995
  • To develope a liquid culture media for the mass culture of Beauveria bassiana blastospore, wheat bran and soybean cake were selected as a carbon and nitrogen source, respectively. The composition and final concentration of wheat bran and soybean cake in liquid culture media were determined by the growth of B. bassiana blastospore. The results showed that the growth of B. bassiana blastospore was effective in the rate of 3 wheat bran: 2 soybean cake and in 5% of final concentration of the liquid culture media, suggesting that the liquid culture media using wheat bran and soybean cake are available.

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Candida magnoliae SR101에 의한 Erythritol의 생산에서 산업용 질소원의 선정 및 최척화

  • Park, Seon-Yeong;Seo, Jin-Ho;Yu, Yeon-U
    • 한국생물공학회:학술대회논문집
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    • pp.351-354
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    • 2001
  • In this experiment, we tested various nitrogen sources and then culture condition was optimized for industrial applications. The batch culture of Candida magnoliae SR101 grown in a defined medium supplemented with light steep water (LSW) as a sole nitrogen source showed a relatively high yield of erythritol production (53%), which was slightly higher than that using yeast extract as a nitrogen source, while the productivity and cell mass were maintained at similar levels. For the optimization of culture condition, the batch culture was performed. When the concentration of LSW was 65 mL/L in the defined medium containing 250 g/L of glucose, the concentration, yield and productivity of erythritol were 110 g/L, 44%, and 0.66 g/L-hr, respectively. The high yield and comparable productivity obtained with a cheap nitrogen source could be expected as a basis for the mass production of erythritol in the industrial scale.

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Growth of Axenic Rotifer Brachionus rotundiformis (무균 로티퍼 Brachionus rotundiformis의 증식)

  • Jung, Min-Min;Rho, Sum;Kim, Pil-Yun
    • Journal of Aquaculture
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    • v.11 no.1
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    • pp.91-97
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    • 1998
  • This paper introduces to a simple culture method and growth of axenic (bacteria-free) rotifer Brachionus rotundiformis for seed stock of rotifer mass culture. This rotifer axenic culture method is based on the washing and transferring with sterilized sea water and modified antibiotic mixture AM9 solution. Population growth (final density on day 16) of axenic cultured rotifer were maintained with a high density and stable growth compared with the control of non-axenic culture (general culture style) through the 3 times-rerunning experiments (trial 1, 2 and 3). But the egg carrying rates of amictic females were not different between the axenic-and non-axenic culture condition. Although, rotifer density was higher in axenic culture, the food (Nannochloropsis oculata) was still remained unutilized than that of the non-axenic culture in third trial culture. These results suggest the possible existence of harmful bacteria for the rotifer population growth in the trial 1 and 3 of non-axenic culture compared to the trial 2. This axenic rotifer culture method is valuable for seed stock of the stable rotifer mass cultures.

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MEDIA DEVELOPMENT FOR MASS PRODUCTION OF ENTOMOPATHOGENIC NEMTOIDE HETERORHABDITIS BACTERIOPHORA AS AN INSECTICIDE

  • Yoo, Sun-Kyun;Cho, Sung-Young;Kim, Seung-Jai;Randy Gaugler
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • pp.107-110
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    • 2001
  • The biological control potential of entomopathogenic nematodes (EPN) can be enhanced by improved culture efficiency. Optimization of media is a key factor for improving in vitro mass production of entomopathogenic nematodes. EPN yield was dependant of complex medium concentration, of which mixture is carbohydrates, lipids, proteins, salts, and growth factors, on the growth of Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescensLipids.

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