• Title/Summary/Keyword: Mass culture

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Medium compositions reveal potential organogenesis in the diploid and tetrploid Codonopsis lanceolata

  • Kwon, Soo Jeong;Hwang, Ha Nule;Moon, Young Ja;Cho, Gab Yeon;Boo, Hee Ock;Lee, Moon Soon;Woo, Sun Hee;Kim, Hag Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.169-169
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    • 2017
  • Medium composition plays a key role on influencing organogenesis in plant tissue culture. This study was carried out to examine the effects of medium composition on organogenesis in diploid and tetraploid Codonopsis lanceolata and obtain in-vitro mass propagation of superior species of C. lanceolata. Diploid C. lanceolata was found to be declined regarding MS medium composition for each concentration. However, shoot and adventitious root formation were suppressed with higher mineral salt concentration, and active growth of shoot and adventitious root was exhibited as 4.9 cm and 3.2 cm respectively in 1/2 MS medium. While in tetraploid C. lanceolata, it showed 2.9 cm and 3.2 cm respectively in 1/4 MS medium. In the case of sucrose concentration, no consistent decrease was observed for growth of shoot and the adventitious root of diploid both at high and low concentration. The growth of shoot (at 3% concentration) and adventitious root (at 7% concentration) was 2.3 cm and 2.0 cm respectively. Although there was no difference in shoot formation of tetraploid C. lanceolata in all concentrations with the range of 1.7~1.8, there was a slight decrease in shoot growth at high concentration. Results revealed that the adventitious root formation was suppressed at high concentration. The concentration of agar exhibited no significant difference in shoot formation of diploid C. lanceolata at all concentrations. The maximum result of adventitious growth (4.1 cm) was observed at 0.8% concentration. Slight inhibition of shoot formation and root formation of tetraploid C. lanceolata was observed at higher concentration. Shoot formation of diploid C. lanceolata also exhibited inhibition at higher concentration. Shoot formation of diploid C. lanceolata was increased at lower pH and shoot growth was the highest (2.3 cm) at pH 3.8. Adventitious root formation was higher at lower pH. However, both the adventitious root formation and growth exhibited comparatively higher result at pH 5.8. Taken together, the levels of pH had an effect on shoot and root formation in diploid and tetraploid of C. lanceolata

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Risk Factors for Complications after Reconstructive Surgery for Sternal Wound Infection

  • Hashimoto, Ichiro;Takaku, Mitsuru;Matsuo, Shinji;Abe, Yoshiro;Harada, Hiroshi;Nagae, Hiroaki;Fujioka, Yusuke;Anraku, Kuniaki;Inagawa, Kiichi;Nakanishi, Hideki
    • Archives of Plastic Surgery
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    • v.41 no.3
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    • pp.253-257
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    • 2014
  • Background Although the utility of flaps for the treatment of sternal wound infections following median sternotomy has been reported for 30 years, there have been few reports on the risk factors for complications after reconstruction. The objective of this investigation was to identify factors related to complications after the reconstruction of sternal wound infections. Methods A retrospective analysis of 74 patients with reconstructive surgery after sternal wound infection over a 5-year period was performed. Clinical data including age, sex, body mass index (BMI), comorbidities, bacterial culture, previous cardiac surgery, wound depth, mortality rate, type of reconstructive procedure, and complication rate were collected. Results The patients' BMI ranged from 15.2 to $33.6kg/m^2$ (mean, $23.1{\pm}3.74kg/m^2$). Wound closure complications after reconstructive surgery were observed in 36.5% of the cases. The mortality rate was 2.7%. Diabetes mellitus significantly affected the rate of wound closure complications (P=0.041). A significant difference in the number of complications was seen between Staphylococcus aureus (S. aureus) and coagulase-negative Staphylococci (P=0.011). There was a correlation between harvesting of the internal thoracic artery and postoperative complications (P=0.048). The complication rates of the pectoralis major flap, rectus abdominis flap, omentum flap, a combination of pectoralis major flap and rectus abdominis flap, and direct closure were 23.3%, 33.3%, 100%, 37.5%, and 35.7%, respectively. Conclusions Diabetes mellitus, S. aureus, harvesting of the internal thoracic artery, and omentum flap were significant factors for complications after reconstruction. The omentum flap volume may be related to the complications associated with the omentum flap transfer in the present study.

Establishment of tissue culture and acclimation of white balloon flower (Platycodon grandiflorum DC. cv. Jangback) for the raising of in vitro propagated seedlings (장백도라지의 대량 증식을 위한 조직배양 및 순화 조건 확립)

  • Han, Eun-Heui;Son, Yong-Wan;Kim, Man-Bae;Shin, Yong-Wook;Cho, Young-Son;Lee, Shin-Woo
    • Journal of Plant Biotechnology
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    • v.41 no.3
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    • pp.134-139
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    • 2014
  • The aim of this study was to establish the condition of regeneration for white balloon flower (Platycodon grandiflorum DC. cv. Jangback) and to manage for the raising of seedling with in vitro regenerated plants. It was examined that 0.5 mg/L of NAA and 1.0 mg/L of BA was the best composition for the callus and shoot induction (up to 600%). NAA was better than IBA for the induction of root and it took 16.9 days for the induction of rooting on the MS soild media containing 0.5 mg/L of NAA and the final rooting ratio was up to 75%. Out of 5 different bed soils purchased from local market, "Tosil" was identified to be the best for the acclimation and growth of in vitro regenerated balloon flower. In detail, on 8 weeks after planting of in vitro regenerated plants in pots containing "Tosil" bed soils, the plant hight was increased up to 2-fold (12.8 cm), 3.5-fold (27) for the number of leaf and 1.5-fold (4.5 cm) for the leaf length when compared to the other four bed soils, respectively. Our preliminary results indicate that it is possible to prevent the occurrence of blue balloon flower in the massive cultivated area of white balloon flower by providing the seedlings raised from in vitro regenerated plants.

A Study on the Growth Characteristics of Commercially Developed Nitrifying Bacteria and its Application to Activated Sludge Process (상업용 질산화 박테리아의 성장특성과 활성슬러지 공정에서의 적용 방법에 따른 연구)

  • Whang, Gyu-Dae;Lee, Bong-Hee
    • Journal of Korean Society of Water and Wastewater
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    • v.20 no.4
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    • pp.595-604
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    • 2006
  • The growth characteristics of Commercially Developed Nitrifying Bacteria (CDNB) were studied in laboratoryscale. CDNB, a pure, artificially isolated bacterium, was cultivated to produce Cultivated Nitrifying Bacterium Group (CNBG). The average ammonia removal rate of CDNB was 0.0234g $NH_4^+-N/g$ MLSS/hr. CNBG was produced in the batch reactor and Specific Nitrification Rate (SNR) was determined at 0.0107g $NH_4^+-N/g$ MLSS/hr. The SNR of CNBG was lower than the SNR of CDNB because the diverse and multi-cultured microbial growth took place during cultivation. The effect of the temperatures and the mixing ratios of sewage and culture solution on the SNR of CNBG was studied. The SNR of CNBG, 0.0107g $NH_4^+-N/g$ MLSS/hr at $27^{\circ}C$, decreased to 0.0048g $NH_4^+-N/g$ MLSS/hr at $15^{\circ}C$, and temperature coefficient (${\Theta}$) was calculated to be 1.07. With the varied sewage mixing ratios, the SNR of CNBG remained unchanged. Activated sludge reactors maintaining an MLSS of 2,000mg/L at HRT of 4 h were operated under conditions in which dosage of Concentrated CNBG Solution (CCNBGS, 10,000mg MLSS/L) and application method of CNBG were varied. The reactor with 20mL of CCNBGS took shorter time to oxidize $NH_4^+-N$ reaching 1mg/L than the reactor with 5mL of CCNBGS showing that higher dosages were associated with greater mass removal of $NH_4^+-N$. However, the total removal was not great. In terms of different methods of CNBG application, reactor seeded with 20mL of CCNBGS took 3days to reach 1mg/L of effluent ammonia concentration while reactor dosed with 20% (v/v) CNBG implanted media took 2days. Both the control reactor and the reactor dosed with 20% (v/v) media only did not reach 1mg $NH_4^+-N/L$ after operating 18days. The reactor with CNBG implanted media had the highest $NH_4^+-N$ removal rate because of maintaining high concentration of Nitrifying Oxidizing Bacteria (NOM), and is regarded as an appropriate method for the activated sludge process.

The Growth and EPA Synthesis of Shewanella oneidensis MR-1 and Expectation of EPA Biosynthetic Pathway

  • Jeong, Young-Su;Song, Sang-Kyu;Lee, Su-Jin;Hur, Byung-Ki
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.2
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    • pp.127-133
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    • 2006
  • Shewanella oneidensis MR-1 has the ability to inhale certain metals and chemical compounds and exhale these materials in an altered state; as a result, this microorganism has been widely applied in bioremediation protocols. However, the relevant characteristics of cell growth and biosynthesis of PuFAs have yet to be thoroughly investigated. Therefore, in this study, we have attempted to characterize the growth and fatty acid profiles of S. oneidensis MR-1 under a variety of temperature conditions. The fastest growth of S. oneidensis MR-1 was observed at $30^{\circ}C$, with a specific growth rate and doubling time of $0.6885h^{-1}\;and\;1.007 h$. The maximum cell mass of this microorganism was elicited at a temperature of $4^{\circ}C$. The eicosapentaenoic acid (EPA) synthesis of S. oneidensis MR-1 was evaluated under these different culture temperatures. S. oneidensis MR-1 was found not to synthesize EPA at temperatures in excess of $30^{\circ}C$, but was shown to synthesize EPA at temperatures below $30^{\circ}C$. The EPA content was found to increase with decreases in temperature. We then evaluated the EPA biosynthetic pathway, using a phylogenetic tree predicted on 16s rRNA sequences, and the homology of ORFs between S. oneidensis MR-1 and Shewanella putrefaciens SCRC-2738, which is known to harbor a polyketide synthase (PKS)-like module. The phylogenetic tree revealed that MR-1 was very closely related to both Moritella sp., which is known to synthesize DHA via a PKS-like pathway, and S. putrefaciens, which has been reported to synthesize EPA via an identical pathway. The homology between the PKS-like module of S. putrefaciens SCRC-2738 and the entire genome of S. oneidensis MR-1 was also analyzed, in order to mine the genes associated with the PKS-like pathway in S. oneidensis MR-1. A putative PKS-like module for EPA biosynthesis was verified by this analysis, and was also corroborated by the experimental finding that S. oneidensis MR-1 was able to synthesize EPA without the expression of $dihomo-{\gamma}-linoleic$ acid (DGLA) and arachidonic acid (AA) formed during EPA synthesis via the FAS pathway.

Effects of Substance P on the Release of Cytokines from Immune Cell Lines (면역세포의 cytokine 유리에 미치는 substance P의 영향)

  • Lee, Jin-Yong;Kim, Soo-Ah;Seo, Seok-Ran;Kim, Hyong-Seop
    • Journal of Periodontal and Implant Science
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    • v.27 no.2
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    • pp.425-441
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    • 1997
  • The neuropeptide substance P(SP) has been implicated in the mediation of inflammation and immune-mediated disease such as arthritis. Recently, it was reported that SP was markedly increased around the blood vessels in inflamed gingiva as well as in close association with the inflammatory cell infiltrate. These results support that SP may contribute to the pathophysiology of neuronal inflammation in human periodontal tissues. SP may regulate inflammatory/immune responses by stimulating the proliferation of human T cells, differentiation and antibody-secreting potential of B cells, macrophage respiratory burst, connective tissue proliferation, and the secretion of cytokines from monocytes and T cells. Here, I studied potential role of SP as a costimulatory chemical signal in inflammatory/immune responses, by determining the released proinflammatory cytokines such as $MIP-1{\alpha}$, $IL-1{\beta}$, and IL-6 from culture supernatants of homogeneous immune cell lines. Serum free cell supernatants were concentrated with TCA precipitation, fractionated with SDS-PAGE, and subjected into western blot analysis. Among 15 cell lines tested, macrophage/monocyte cell line RAW264.7 and WRl9m.1 showed the highest level of induction of $MIP-1{\alpha}$ when stimulated with LPS. Discrete IL-6 bands with multiple forms of molecular mass were detected from supernatants of B cell lines A20(32kDa), Daudi(32, 35kDa), and SKW6.4(29kDa), which were expressed constitutively. $IL-1{\beta}$ could not be detected by the method of western blot analysis from supernatants of all cell lines tested except RAW264.7, WRl9m.1, and erythroid cell line K562 which showed the least amount of $IL-{\beta}$ secretion. SP $10^{-9}M$ with suboptimal dose of LPS treatment showed synergistic induction of $MIP-1{\alpha}$ release from RAW264.7 or WR19m.1, and also IL-6 release from A20, but this synergism is not the case in costimulation of RAW264.7 or WRl9m.1 with SP $10^{-9}M$ and TPA. Although treatment of T cell line CTLL-R8 with SP $10^{-7}M$ or PHA+TPA induced modest level of $MIP-1{\alpha}$ secretion, synergism was not observed when they are applied together. These findings all together suggest the possibility of a regulatory role of SP in inflammatory/immune reaction through differential modulation of bioactivities of other chemical cosignals.

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Effect of Drinking and Smoking on Skin Health and Make-up (음주.흡연습관이 피부건강 화장습관에 미치는 영향)

  • Lee, Sun-Hee;Choi, Ga;Na, Young-Soon
    • Korean Journal of Human Ecology
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    • v.13 no.2
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    • pp.263-269
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    • 2004
  • This study was conducted with the aim of providing the basic data for an informative and educational program designed to give a guideline how to keep and care the skin healthy and proper. Female freshmen, enrolled in colleges in Jeonbuk provincial area, were surveyed about their status of health and way of skin care and make-up. The results of research were summarized as follows: First, 268 students(65.4%) were 19 years old or younger. Among the respondents, 219 persons(54.4%) lived in small or medium cities and 271(66.1%) showed less than 20 of body mass index($kg/m^{2}$). Second, there were statistically significant correlations between dwelling place, drinking habit(p<0.05), and subjective health status(p<0.05) with health, skin care and make-up habit. Third, drinking, subjective health status appeared to be significant variables affecting facial skin(p<0.05) and health status(p<0.01), respectively. Forth, smoking and regular meal seemed to affect the length of make-up(p<.05) and smoking esd strongly connected with the extent of make-up. The way of thinking about cosmetics were affected by the habits of drinking(p<0.05) and smoking(p<0.01), respectively. Drinking habit was the significant variable of health affecting on the belief in the effect of cosmetics. Fifth, skin health was negatively correlated with drinking and smoking habits and positively with subjective health status, BMI($kg/m^{2}$) and frequency of make-up. Sixth, healthier state of facial skin appeared to be happened with statistical significance(p<0.01) in rural areas than in urban areas and with a person of higher than 20 of BMI($kg/m^{2}$) than of less than 20 from the regression analysis. It has been estimated that there were meaningful correlations between the status of health and make-up among female freshmen. Accordingly, the concept of health reinforcement can be adopted to skin care and skin maintenance, so various and comprehensive approaching methods were required to improve and reinforce the skin management and cosmetic culture.

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An Exploratory Study on the Buying Decision-making Process of Automobile Books (자동차전문서적 구매의사 결정과정에 관한 탐색적 연구)

  • Kim, Kil-Hyun;Ha, Kyu-Soo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.6 no.3
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    • pp.1-18
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    • 2011
  • Publication Industry of scientific technology is showing tendency of decreasing in sales, which clearly draws down curve since 2000. The printing culture is declining whether it is because of trend avoiding pure science or technological science and engineering, advancement of mass media or increasing frequency of using internet. Nevertheless, researcher considered car industry in publication industry as a life-long purpose for study. For this reason, the researcher tried to find the variables of the marketing which give impact on the customers such as student group and consumer who buys cars, when buying professional books. The found variables are expected to have a huge impact on the publication industry of professional books. As a result of research, in the area of the vision and motive, most said that they have "chosen a major in car because they liked car in usual base." In the stage of recognizing the problem, they buy the books when it is inevitably necessary. In the stage of searching for the information, they get information from advertisements, friends, professors, internet or sales clerk in book stores. In the stage of evaluation, they look for the title and the publication date of the books. In addition, in the stage of deciding purchase, "buy immediately" was the most frequent answer while impulsive purchase is the least frequent answer. In the stage of evaluating after purchase, many of them mostly are satisfied with their purchase.

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Mineralogical Characteristics and Provenance of Cu-bearing Green Minerals Used as Traditional Pigments (전통 안료로 사용된 구리함유 녹색광물의 광물학적 특성과 산지추정)

  • Do, Jin-Young;Jung, Jongmee
    • Journal of the Mineralogical Society of Korea
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    • v.31 no.2
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    • pp.123-135
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    • 2018
  • This study tried to find the clue to Seokrok province by comparing Seokrok used in painting culture properties with Seokrok ore from domestic occurrence and imported Seokrok ore. To this end, chemical and mineralogical characteristics of painting cultural properties were identified with portable X-ray Florescence (p-XRF), micro X-ray diffraction (micro XRD) and SEM/EDS Analysis. To obtain Pb isotopic ratio, the Pb contained in Seokrok has been analyzed with Thermal Ionization Mass Spectrometer. Atacamite (or botallackite) and small quantity of brochantite were identified from Seokrok in Dancheong, and malachite was also identified from Buddhist painting besides those two ingredients. Without distinction of type, most Seokrok used in painting cultural properties is atacamite composed of Cu and Cl. From Pb isotope analysis, it was found that Seokrok in painting cultural properties was closer to that of north Korea, north China and Japan than south Korea as in regional division for East North Asia suggested by Mabuchi. The Pb isotopic ratio of domestic green mineral belongs to the distribution of Seokrok inside the painting cultural properties but imported malachite showed considerably difference. Considering the fact that atacamite, the main mineral of Seokrok in painting cultural properties is rarely produced from southern mine of the Korean Peninsula and the result of Pb isotope analysis.

화학독립영양미생물 Aeromonas sp. strain JS-1의 RubisCO 정제 및 특성

  • Na, Suk-Hyeon;Bae, Sang-Ok;Kim, Min-Jeong;Kim, Seong-Jun;Jeong, Seon-Yong
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.461-464
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    • 2002
  • A Chemoautotroph identified as an Aeromonas sp. strain JS-1 was isolated from fresh water. Aeromonas sp. strain JS-1 used the $H_2$ and $CO_2$ as energy and carbon sources, respectively. Growth characteristics for improving the $CO_2$ fixation rate were examined in batch cultivation. Its results shown that the optimal growth appeared at culture conditions of $35^{\circ}C$, pH 7 and NaCl 0.1%(w/v). Some hydrogen-oxidizing bacteria were reported that the enzyme activity of ribulose 1,5-bisphosphate carboxylase- oxygenase (RubisCO-EC 4.1.1.39), in the key enzyme of the Calvin-Benson cycle. A RubisCO was purified from a chemoautotrophic bacterium, Aeromonas sp. strain JS-1. the enzyme was purified by ammonium sulfate precipitation, DEAE-sepharose CL-6B and gel filtration chromatography. The RubisCO showed that molecular mass was about 560KDa from gel filtration chromatography and nondenaturing PAGE, and the RubisCO was confirmed to consist of $L_8S_8$ enzyme structure by sodium dodecyl sulfate polyacrylamide gel electrophoresis. A large subunit was about 56KDa and small one was about 15kDa. The Km values of the enzyme for ribulose 1,5-bisphosphate(RUBP), $NaH^{14}CO_3$, and $Mg^{++}$ were estimated to be 0.25mM, 5.2mM, and 0.91mM, respectively. The optimum temperature for RubisCO enzymatic activity were $50^{\circ}C$, and the enzymatic activity was stable up to $45^{\circ}C$.

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