• Title/Summary/Keyword: Mass cultivation

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Isolation of a Fermenting Microorganism Involved in Formation of ortho-Dihydroxyisoflavones in Doenjang (Korean Fermented Soybean Paste)

  • Seo, Hyo-Seel;Lee, Jae-Hwan;Kwon, Dae-Yong;Park, Jin-Byung
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.1030-1034
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    • 2009
  • A fermenting microorganism involved in formation of ortho-dihydroxyisoflavones (ODIs) during aging of doenjang (Korean fermented soybean paste) has been investigated. Microorganisms in ODI-containing doenjang were isolated by cultivating on yeast mold (YM) agar medium containing 0-7% NaCl. ODI formation of the isolated strains was examined by gas chromatography/mass spectrometry (GC/MS) analysis after cultivation in modified YM broth or soybean extract medium. An ODI-producing microbe was identified as Bacillus subtilis HS-1 based on 16S rRNA gene sequence analysis. The strain has produced 8-hydroxydaidzein as a major product during growth in the modified YM broth or soybean extract medium. Therefore, it was concluded that one of the microorganisms involved in the formation of ODIs in doenjang was B. subtilis HS-1.

A Study on the Water Quality Management in Fallow Paddy Fields (휴경논에서의 수질관리 연구)

  • Kim Hyung-Jong;Kim Sun-Joo;Kim Phil-Shik;An Yeul;Yang Yong-Suck
    • Journal of The Korean Society of Agricultural Engineers
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    • v.48 no.2
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    • pp.69-76
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    • 2006
  • Fallow paddy areas have been increased due to the import of cheap agricultural product, and the unbalance between farming cost and rice price since 1990. The increasing fallow paddy area needs to be protected from the devastation by weed breeding for the re-cultivation. In this study, two fallow paddy fields managed with different water depth were selected for monitoring and analysing of water quality, water balance and plant body change. The managed fallow paddy fields were more effective in water quality purification and plants growth control than non-managed fallow paddy fields. And the fallow paddy field managed with some degree of water depth was the most effective field in terms of weed control.

Isolation and Characterization of a Novel Agarase-Producing Pseudoalteromonas spp. Bacterium from the Guts of Spiny Turban Shells

  • Oh, Young-Hoon;Jung, Chang-Kyou;Lee, Jin-Won
    • Journal of Microbiology and Biotechnology
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    • v.21 no.8
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    • pp.818-821
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    • 2011
  • An agar-degrading bacterium was isolated from the guts of spiny turban shells. It was identified as a Pseudoalteromonas species and named Pseudoalteromonas sp. JYBCL 1. The viscosity of the inoculated agar medium decreased by more than 60% after 20 h cultivation. The agarase produced by the isolate had optimal activities at $35^{\circ}C$ and pH 7. The enzyme had extremely strong resistance to ionic stress compared with other known agarases. Its molecular mass was estimated at about 60 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The agarase could saccharify Gelidium amansii directly, with an efficiency about half that compared with agar saccharification.

Enhanced Cell Growth of Chlorella sp. KR-l by the Addition of Iron and EDTA

  • Sung, Ki-Don;Lee, Jin-Suk;Shin, Chul-Seung;Park, Soon-Chul
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.409-411
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    • 1998
  • The effects of iron and EDTA on the growth of Chlorella sp. KR-1, a highly$CO_2$tolerant fresh water micro alga, have been determined. The algal growth was significantly affected not only by iron concentrations in the medium but by the ratio of iron to EDTA. The linear growth rate and the final cell concentration are increased with the supplementation of EDTA. Enhanced growth of Chlorella sp. KR-1 by the supplementation of EDTA was mainly due to the fact that the supply of iron to the algal culture had been possible for a longer time. When Chlorella sp. KR-1 is cultured in the medium of iron-15H-EDTA, the linear growth rate and the final cell concentration are at their maximum, 0.88 g/l${\cdot}$day and 9.1 g/l, respectively. The results show that Chlorella sp. KR-1 may be used for mass cultivation to fix$CO_2$from stack gases.

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A Survey on Perceived Food Safety in Urban Residents (일부 수도권 거주 주민의 식품 안전성에 대한 인식 조사)

  • Nam Hee-Jung;Kim Young-Soon
    • The Korean Journal of Food And Nutrition
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    • v.19 no.2
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    • pp.126-142
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    • 2006
  • A survey was conducted to investigate the relationship between knowledge of perceived food safety and actual food purchase practices among parents of children under the age of seven (N=309) in 2005. The seventeen following perceived food safety items were included in the survey (bovine spongiform encephalopathy, foot and mouth disease, pathogenic avian influenza, swine fever, allergens, GMO, irradiated food, food poisoning bacteria, endocrine disrupter, antibiotics, food additives, carcinogen, natural toxin, agrichemical residues, place of origin, cultivation methods, and heavy metal) Most parents were highly concerned about investigating all food safety topics. Up to 90.0% of the participants recognized the importance of food safety. The public concern has caused a considerable decline in the demand for food afterreceiving news about food safety through mass media, Also, the majority of subjects required correct food safety information.

On-line Analysis of Phellinus linteus WI-001 Fermentation Parameters. (Phellinus linteus WI-001 발효에 배양공정 parameter의 온라인 분석)

  • 김종래;권호균;전계택;이계관
    • Microbiology and Biotechnology Letters
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    • v.28 no.5
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    • pp.298-302
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    • 2000
  • Fermentation parameters were estimated by use of a vent gas analyzer coupled to a computer data acquisition system in cultivation of Phellinus linteus WI-001, pro-ducer of polysaccharides known to have potent anticancer activities. Oxygen uptake rate(OUR), a critical indicator of the cells activities, was calculated by applying oxygen mass balance. In addition, by dividing the oxygen uptake rate hy the total oxygen consumed, on-line estimation of the cells specific growth rate was successfully done. It was also possible to estimate cell concentration directly bt use of oxygen-cell yield($Y_{x/o}$ ) which was obtained based on a correlation between cell growth and total oxygen consumed.

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Soil compaction and tractive force of big tractors and caterpillars (challenger 65 &45)

  • Weibbach, Michael
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.404-412
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    • 1996
  • For the cultivation of big agricultral areas high performance machinery is needed. The need to increase working productivity also leads to powerful machinery. The total weight of new tractor generations increases and with it their axle and wheel weight reaches a soil damaging extend. Besides carrying the mass of the tractors and harvesting-machines the chassis must transmit driving force onto the ground(Tab. 1). To decrease soil damaging compaction a number of technical solutions have been developed. Broad tyres are being used to minimize the contact-area pressure by using low tyre air pressure. For enlarging the contact area there are two-possibilities : to build a brought or a long contact area. done for instance in caterpillar tracks.

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Analysis of Index Components in Medicinal Crops of Andong Area Cultivated at Different Altitude (안동지역 약용작물의 표고별 지표성분 분석)

  • An, Hui Jeong;Chung, Koo Min
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.2
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    • pp.91-97
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    • 2014
  • The contents of index components in 5 medicinal crops cultivated at different heights in Andong area. The contents of allantoin in Dioscorea batatas Decne and catalpol in Rehmannia glutinosa Liboschitz were not different by cultivation heights (100 ~ 200m). But the contents of index components in Astragalus membranaceus and Angelica acutiloba Kitagawa were higher in samples cultivated at elevated heights. At 700 m of heights, the contents of formononetin and astragaloside I in Astragalus membranaceus were 1.20 and $201.2{\mu}g/g$, respectively. The contents of decursin and nodakenin in Angelica acutiloba Kitagawa were 68.3 and 4.20 mg/g, respectively. Thus Astragalus membranaceus and Angelica acutiloba Kitagawa are recommended to be cultivated in higher altitude.

Microbial Symbiosis in Marine Sponges

  • Lee, Yoo-Kyung;Lee, Jung-Hyum;Lee, Hong-Kum
    • Journal of Microbiology
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    • v.39 no.4
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    • pp.254-264
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    • 2001
  • Sponges are host organisms for various symbiotic microorganisms such as archaea, bacteria, cyano-bacteria and microalgae. Sponges are also sources of a wide variety of useful natural products like cyto-toxins. antifouling agents, antibiotics, and anti-inflammatory and antiviral compounds, Symbiotic microorganisms is sponges can be sources of various natural products, because metabolites previously ascribed to sponges have recently been demonstrated to be biosynthesized by symbionts. If a symbiotic microorganisms from which some natural products are derived can be cultured, the microorganism could be used in a mass production of the bioactive comopounds. We summarize recent research on iso-lation and cultivation of sponge-symbiotic microorganisms and the symbiotic relationship.

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Carbon dioxide fixation by microalgae photosynthesis (미세조류에 의한 $CO_2$ 고정화 연구)

  • 성기돈;이진석;이준표;김미선;박순철
    • Proceedings of the Korea Society for Energy Engineering kosee Conference
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    • 1995.05a
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    • pp.82-87
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    • 1995
  • Carbon dioxide fixation by microalgae has several advantages such as no requirements for the pretreatment over phycal and/or chemical $CO_2$ fixation processes. Chlorella sp. HA-1, fresh algae. and Chlorococcum littorale. marine algae, were used to do $CO_2$ fixation work. The experimental parameters were light intensity and $CO_2$ concentration. Chlorella sp. HA-1 has the maximum growth rate at 8 Klux and 10% $CO_2$ concentration. Chlorococcum littorale showed the maximum growth at similar conditions. The results indicated the feasibility of the Chlorella HA-1 and Chlorococcum littorale for mass cultivation using flue gas.

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