• Korean Journal of Applied Biomechanics
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• v.28 no.1
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• pp.29-35
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• 2018

Objective: The purpose of this study was to determine the asymmetry of vertical ground reaction force (GRF) components between dominant and non-dominant legs in rested and fatigued states in prolonged running. Method: Twenty healthy men, heel strikers, were included (age: $24.00{\pm}5.0years$; height: $176.1{\pm}6.0cm$; body mass: $69.0{\pm}6.0kg$) in this study. Subjects ran on an instrumented treadmill for 130 minutes. During treadmill running, GRF data (1,000 Hz) were collected for 20 strides at five minutes (rested) and 125 minutes (fatigued) running while they were unaware of collecting data. Asymmetry indexes (ASI) were calculated to quantify the asymmetry magnitude in rested and fatigued states. Paired t-test was used to verify the differences between dominant and non-dominant legs in rested and fatigued states. In addition, one-way repeated measure analysis of variance was applied for comparison of ASI of both states. The level of significance was set at p < .05. Results: Passive force peak magnitude, loading rate, and impulse affecting the development of running injury were found significantly greater in dominant leg than in non-dominant leg at rested state (p < .05). However, passive force peak time and active force peak magnitude were found significantly different between legs in fatigued state (p < .05). To determine changes in percentage of asymmetry between legs in both states, ASI was used. ASI for all variables increased in fatigued state; however, no significant differences were found between both states. Conclusion: This study found that fatigue did not affect differences in vertical GRF between dominant and non-dominant legs and asymmetry changes.

• Korean Journal of Applied Biomechanics
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• v.28 no.1
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• pp.37-43
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• 2018

Objective: The aim of this study was to analyze muscle activation of the lower extremities as a function of changes of the center of pressure (CoP) of the foot during squats in order to provide quantitative information to trainers who would like to teach correct movements for developing muscles. Method: Ten men with over three years of weight training experience participated in this study (age: $26.1{\pm}0.8yrs$, height: $171.2{\pm}3.9cm$, body mass: $71.1{\pm}5.7kg$, 60%RM: $84{\pm}9kg$, career: $4.0{\pm}0.7yrs$). The participants were instructed to perform a squat in each of 3 conditions, with different CoP's (the front, middle, and rear of the foot). Results: The position of the CoP showed significant differences according to instructions in both the eccentric and concentric contraction phases (p < .05). The range of movement of the hip and ankle joints showed significant differences corresponding to changes of the CoP position (p < .05). The rectus femoris and gluteus maximus muscle showed significant differences for different CoP positions only in the concentric contraction phase, while the gastrocnemius and anterior tibialis were significantly different in both the concentric and eccentric contraction phase (p < .05). Conclusion: When the target muscle of squat training is the gastrocnemius, the CoP should be located in the front of the foot for effective muscle training. When the target muscles of squat training are the gluteus maximus and quadriceps femoris, the CoP should be located on the rear of the foot.

• Korean Journal of Applied Biomechanics
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• v.23 no.3
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• pp.209-218
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• 2013

This study was to perform the kinetic analysis of forward $1\frac{1}{2}$ somersault on the platform diving. Six men's diving players of the Korea national reserve athletes participated in this study. The variables were analyzed response time, velocity, center of mass (COM), angle, center of pressure (COP) and ground reaction force (GRF) of motion. For measure and analysis of this study, used to synchronized to 4 camcorder and 1 force plate, used to the Kwon3D XP (Ver. 4.0, Visol, Korea) and Kwon GRF (Ver. 2.0, Visol, Korea) for analyzed of variables. The results were as follows; Time factor were observed in maximum knee flexion depending on the extent of use at phase 1 of take-off to execute the somersault. This enabled the subject to secure the highest possible body position in space at the moment of jumping to execute the somersault and prepare for the entry into the water with more ease. Regarding the displacement of COM, all subjects showed rightward movement in the lateral displacement during technical execution. Changes in forward and downward movements were observed in the horizontal and vertical displacements, respectively. In terms of angular shift, the shoulder joint angle tended to decrease on average, and the elbow joints showed gradually increasing angles. This finding can be explained by the shift of the coordinate points of body segments around the rotational axis in order to execute the half-bending movement that can be implemented by pulling the lower limb segments toward the trunk using the upper limb segments. The hip joint angles gradually decreased; this accelerated the rotational movement by narrowing the distance to the trunk. Movement-specific shifts in the COP occurred in the front of and vertical directions. Regarding the changes in GRF, which is influenced by the strong compressive load exerted by the supporting feet, efficient aerial movements were executed through a vertical jump, with no energy lost to the lateral GRF.

• Korean Journal of Applied Biomechanics
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• v.26 no.4
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• pp.361-367
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• 2016

Objective: The aim of this study was to compare three-dimensional kinematic changes of the lower extremity between the two different braking distances during snowplow in alpine skiing. Method: Six alpine ski instructors (age: $25.3{\pm}1.5yr$, height: $169.3{\pm}2.9cm$, weight: $66.2{\pm}5.9kg$, career: $4.2{\pm}2.9yr$) participated in this study. Each skier was asked to perform snowplow on the two different braking distances (2 and 4 m). Results: Snowplow and edging angles (p = .006 and p = .005), ankle adduction and inversion (p = .033 and p = .002), knee extension (p = .003), and hip abduction and internal rotation (p = .043 and p = .006) were significantly greater in the 2 m than in the 4 m braking distance. Conclusion: Based on our results, we suggest that skiers should make greater snowplow and edging angles on the shorter braking distance. In this situation, ankle joint adduction/inversion angle and hip joint internal-rotation make greater snowplow angle, and hip joint abduction make greater edging angle. In addition, greater knee joint extension angle may lead to more posteriorly positioned center of mass.

• Toxicological Research
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• v.28 no.4
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• pp.269-277
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• 2012

The purpose of this study was to understand the mechanism of cardiovascular disease (CVD) caused by exposure to hazardous chemicals. We investigated changes in the symptoms of metabolic syndrome, which is strongly related to CVD, and in levels of other CVD risk factors, with a special emphasis on the roles of catecholamines and oxidative stress. The results revealed that neither body mass index (BMI) nor waist and hip circumferences were associated with exposure to hazardous chemicals. Among metabolic syndrome criteria, only HDL-cholesterol level increased on exposure to hazardous chemicals. Levels of epinephrine (EP) and norepinephrine (NEP) were not influenced by exposure to hazardous chemicals; however, the total antioxidative capacity (TAC) reduced because of increased oxidative stress. Both hazardous chemical exposure level and metabolite excretion were related to EP, NEP, and the oxidative stress index (OSI). Logistic regression analysis with these factors as independent variables and metabolic syndrome criteria as dependent variables revealed that EP was associated with blood pressure, and NEP with metabolic syndrome in the chemical-exposed group. In conclusion, the results suggest that reactive oxygen species generated and oxidative stress due to exposure to hazardous chemicals act as mediators and cause changes in the physiological levels of EP and NEP to increase blood pressure. This ultimately leads to the development of CVD through increase in cholesterol, triglyceride, and blood glucose levels by lipid peroxidation.

• Toxicological Research
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• v.28 no.4
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• pp.217-224
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• 2012

In recent decades, titanium dioxide ($TiO_2$) nanoparticles have been used in various applications, including paints, coatings, and food. However, data are lacking on the toxicological aspects associated with their use. The aim of this study was to assess the inhalation toxicity of $TiO_2$ nanoparticles in rats by using inhalation exposure. Male Wistar rats were exposed to $TiO_2$ nanoparticles for 2 weeks (6 hr/day, 5 days/week) at a mean mass concentration of $11.39{\pm}0.31mg/m^3$. We performed time-course necropsies at 1, 7, and 15 days after exposure. Lung inflammation and injury were assessed on the basis of the total and individual cell counts in bronchoalveolar lavage fluid (BALF), and by biochemical assays, including an assay for lactate dehydrogenase (LDH). Furthermore, histopathological examination was performed to investigate the lungs and nasal cavity of rats. There were no statistically significant changes in the number of BALF cells, results of biochemical assays of BALF and serum, and results of cytokine analysis. However, we did observe histopathological changes in the nasal cavity tissue. Lesions were observed at post-exposure days 1 and 7, which resolved at post-exposure day 15. We also calculated the actual amounts of $TiO_2$ nanoparticles inhaled by the rats. The results showed that the degree of toxicity induced by $TiO_2$ nanoparticles correlated with the delivered quantities. In particular, exposure to small particles with a size of approximately 20 nm resulted in toxicity, even if the total particle number was relatively low.

• Journal of Environmental Science International
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• v.28 no.11
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• pp.1005-1017
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• 2019

Environmental factors and changes in phytoplankton community structure before (August 5, 2017), during (August 18 and 25) and after (August 30 and September 15) freshwater input were analyzed to investigate the effects of freshwater input from Ganwol and Bunam lakes located in the upper part of Cheonsu Bay. Due to the large amount of freshwater input in the Cheonsu Bay, the surface salinity of the bay decreased by more than 8 psu, and the thermocline existing in the bay during August weakened. In addition, hypoxic phenomena occurred temporarily in the bay as the low oxygen water mass from the freshwater lakes flowed into the bay, and chemical oxygen demand, nutrients, and N/P increased with freshwater inflow. The density of phytoplankton during the freshwater inflow increased owing to their input from the freshwater lakes. Diatom species (Eucampia zodiacus) dominated the phytoplankton community in the bay before freshwater input; nanoflagellates, chlorophyta, cyanobacteria, and diatoms (Pseudonitzschia delicatissima, Chateocceros spp.) entered during freshwater input; and after freshwater inflow ended, diatoms (Chateocceros spp.) again became predominant indicating a return to previous conditions. The amount of phytoplankton standing crops increased sharply due to the inflow of freshwater species into the bay on the second day of discharge compared to before freshwater input; pre-discharge conditions were restored at most stations except at some sites close to the Bunam Lake three days after discharge. Therefore, the large amount of freshwater flowing into the bay affects not only the geochemical circulation in the bay but also the phytoplankton community structure. In particular, the high concentration of nutrients in the freshwater lake affect the marine ecosystem of the bay during August.

• Molecules and Cells
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• v.44 no.7
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• pp.500-516
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• 2021

Cardiac hypertrophic signaling cascades resulting in heart failure diseases are mediated by protein phosphorylation. Recent developments in mass spectrometry-based phosphoproteomics have led to the identification of thousands of differentially phosphorylated proteins and their phosphorylation sites. However, functional studies of these differentially phosphorylated proteins have not been conducted in a large-scale or high-throughput manner due to a lack of methods capable of revealing the functional relevance of each phosphorylation site. In this study, an integrated approach combining quantitative phosphoproteomics and cell-based functional screening using phosphorylation competition peptides was developed. A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. Cell-based functional screening against 18 selected phosphorylation sites identified three phosphorylation sites (Ser-98, Ser-179 of Ldb3, and Ser-1146 of palladin) displaying near-complete inhibition of cardiac hypertrophic growth of NRVMs. Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. Our integrated approach can be used to identify functionally important phosphorylation sites among differentially phosphorylated sites, and unlike conventional approaches, it is easily applicable for large-scale and/or high-throughput analyses.

• The Journal of Korean Obstetrics and Gynecology
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• v.32 no.3
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• pp.57-72
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• 2019

Objectives: This study aims to assess the maternal characteristics and changes of body among patients who received Korean medicine postpartum care compared to pre-study. Methods: From January 1, 2018 to February 28, 2019, we included 31 postpartum patients who had body composition analysis at admission and discharge among 34 patients who received postpartum care in ${\square}{\square}$ University Hospital. We used SPSS 21.0 for window to test for statistical significance. Results: The average age, weight, and caesarean section rate increased compared to previous study. The average period of postpartum care has decreased. After postpartum care, abdominal circumference, body weight and Body Mass Index (BMI) was significantly decreased, but not as much as pre-study. The coefficient of determination was derived to predict the effects of postpartum care and calculated as "$Abdominal\;circumference\;change=2.745+0.593{\times}admission\;period$", "$weight\;change=0.214+0.345{\times}admission\;period$" and "$BMI\;change=0.198+0.120{\times}admission\;period$". Conclusions: Compared to previous study, as the maternal age increased and admission period decreased, the weight loss after childbirth decreased. It is necessary to provide individual treatment through coefficient of determination.

• Physical Therapy Rehabilitation Science
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• v.8 no.3
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• pp.170-174
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• 2019

Objective: The objective of this study was to investigate the differences in the quality of life between two groups of patients who received or did not receive total knee replacement (TKR) surgery after being diagnosed with knee osteoarthritis (OA), and to investigate changes in the quality of life for persons who had TKR surgery. Design: Cross-sectional study. Methods: The subjects were randomized into a surgery group (n=70) and a non-surgery group (n=65). Subjects were selected from individuals diagnosed with knee OA from Himchan Hospital in Seoul, South Korea. Their sex, age, weight, height, body mass index, unilateral or bilateral, and quality of life were evaluated. Changes in the quality of life was measured using the Short Form-36 Health Survey (SF-36). Seventy out of 135 patients had TKR surgery, and their quality of life was evaluated at 6 months and 12 months after the surgery. Results: SF-36 scores were significantly improved at 6 months and 12 months after the surgery compared to the scores before the surgery (p<0.05). Also, the comparison between 6 and 12 months after surgery showed that the Vitality and Social Function scores in the SF-36 were significantly increased (p<0.05). Conclusions: The findings of this study showed that TKR surgery has a positive effect on the quality of life for persons with knee OA as a therapeutic intervention.