• 제목/요약/키워드: Mass Science

검색결과 9,460건 처리시간 0.038초

Application of multimodal surfaces using amorphous silicon (a-Si) thin film for secondary ion mass spectrometry (SIMS) and laser desorption/ionization mass spectrometry (LDI-MS)

  • Kim, Shin Hye;Lee, Tae Geol;Yoon, Sohee
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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    • pp.384.1-384.1
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    • 2016
  • We reported that amorphous silicon (a-Si) thin film provide sample plate exhibiting a multimodality to measure biomolecules by secondary ion mass spectrometry (SIMS) and laser desorption/ionization mass spectrometry (LDI-MS). Kim et al.1 reported that a-Si thin film were suitable to detect small molecules such as drugs and peptides by SIMS and LDI-MS. Recently, bacterial identification has been required in many fields such as food analysis, veterinary science, ecology, agriculture, and so on.2 Mass spectrometry is emerging for identifying and profiling microbiology samples from its advantageous characters of label-free and shot-time analysis. Five species of bacteria - S. aureus, G. glutamicum, B. kurstaki, B. sphaericus, and B. licheniformis - were sampled for MS analysis without lipid extraction in sample preparation steps. The samples were loaded onto the a-Si thin film with a thickness of 100 nm which did not only considered laser-beam penetration but also surface homogeneity. Mass spectra were recorded in both positive and negative ionization modes for more analytical information. High reproducibility and sensitivity of mass spectra were demonstrated in a mass range up to mass-to-charge ratio(m/z) 1200 by applying the a-Si thin film in mentioned above MS. Principle component analysis (PCA) - a popular statistical analysis widely used in data processing was employed to differentiate between five bacterial species. The PCA results verified that each bacterial species were readily distinguished and differentiated effectively from our MS approach. It shows a new opportunity to rapid bacterial profiling and identification in clinical microbiology. More details will be discussed in the presentation.

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Effects of Body Build on Metabolic and Physiological Function in Men and Athletes - 1. Especially on the Metabolic Function -

  • Lee, Ok-Hee;Lim, Soon-Gill;Lee, Jung-Hee
    • Nutritional Sciences
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    • 제2권2호
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    • pp.93-101
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    • 1999
  • The aim of this study was to investigate influence of body build on body composition, energy metabolic state and insulin concentration of blood. 29 male athletes and 36 male non-athletic students were recruited for the study. Anthropometry including chest depth and breadth, fat mass, fat fee mass, tricep skinfold thickness were measured. fasting glucose, lactate, triglyceride, fee fatty acid, and insulin concentration in serum were measured . Body build was assessed using metric index, which calculated by regression equations of Mohr and Greil. The athletic and non-athletic students were allocated to 3 body build, that is leptomorph, mesomorph, and pyknomorph. Resting metabolic rate was calculated. Respiratory quotient was determined through ratio of measured VO$_2$, and V$CO_2$. Most non-athletes have a leptomorphic body build, in contrast to athletes mesomorphic type. The body build type influenced body composition differently between non-athletic group and athletic group. Weight, body mass index, body fat mass and fat mass proportion (%), and fat-free mass increased from leptomorph to pyknormorph in non-athletic group. Pyknormorphic athletes have a significant higher body mass index, fat mass, fat free mass than other body build type. Serum glucose, triglyceride, lactate, insulin showed significant differences only in non-athletic group between leptomorph and mesomorph. RMR increased significantly from leptomorph to mesomorph in non-athletes. There was no significant difference of RQ among 3 body build types in both athletes and non-athletes. This study gives a coherent data on body build and body composition for athletes and non-athletes students. The influence of body builds on energy metabolic status of serum was different between athletes and non-athletes.

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분동교환기를 이용한 고체밀도기준물의 질량측정 (Mass measuremeant of soilid density standard using weight exchanger)

  • 이용재;장경호;오재윤;정상덕
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2003년도 춘계학술대회 논문집
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    • pp.1659-1662
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    • 2003
  • The mass measurement of solid density standards using weight exchanger is described. KRISS(Korea Research Institute od Standards and Science) has several solid density standards. Their mass have been measured manually only using a mass comparator(Mettler, 1kg - 0.01mg). However, the uncertaity of the manual mass measurement is up to 300 microgarm much more than 32 microgram of advanced NMIS(National Metrology Institutes) for 1 kg silicon sphere which is primary density standards due to an eccentric error and buoyancy correction error. The new system with a weight exchanger is designed and built to improve the measurement accuracy. It comprises a weight exchager, a mass comparator, air density instruments, and application program for automatic measurement. It is evaluated by measuring several elements in an air tight chamber to verify the performance of it.

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Estimating dark matter mass for the most massive high-z galaxy cluster, SPT-CL J2106-5844 using weak-lensing analysis with HST observations

  • Kim, Jinhyub;Jee, Myungkook James;Ko, Jongwan
    • 천문학회보
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    • 제41권1호
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    • pp.67.2-67.2
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    • 2016
  • SPT-CL J2106-5844 is known to be one of the most massive galaxy clusters ($M_{200}{\sim}1.27{\times}10^{15}M_{sun}$) ever found at z > 1. Given its redshift (z ~ 1.132), the mass of this cluster estimated by Sunyaev-Zel'dovich effect and X-ray observation is too large compared with the current ${\Lambda}CDM$ cosmology prediction. Mass estimation from these methods can be biased because they require assumptions on hydrostatic equilibrium, which are not guaranteed to hold at such high redshift (about 40% of the current age of the Universe). Thus, we need to verify the mass of this interesting cluster using gravitational lensing, which does not require such assumptions. In this work, we present our preliminary result of dark matter mass and its spatial mass distribution of SPT-CL J2106-5844 using weak-lensing analysis based on HST optical/NIR deep imaging data. We compare mass estimates from different sources and discuss cosmological implications.

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Embedded System을 위한 iRTOS$^{TM}$기반의 USB Mass Storage 설계 및 구현 (Design and Implementation of USB Mass Stowage Based on iRTOS$^{TM}$ for Embedded System)

  • 안희중;한성용;박희상;이철훈
    • 한국정보과학회:학술대회논문집
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    • 한국정보과학회 2002년도 봄 학술발표논문집 Vol.29 No.1 (A)
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    • pp.97-99
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    • 2002
  • 최근 PC 시장은 USB라는 새로운 인터페이스의 보급으로 이용한 장치들이 급속도로 증가하고 있다. 또한 USB를 이용하여 저장 장치를 접근할 수 USB Mass Storage는 디지털 카메라나 MP3 Player 등에서 빠른 전송속도와 편리함 때문에 각광 받고 있다. 본 논문에서는 실시간 운영 체제인 iRTOS$^{TM}$에서 USB 인터페이스를 이용한 USB Mass Storage가 가능하도록 설계하고 이를 구현한 내용을 기술한다.

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Characterization of Modified Peptides by Tandem Mass Spectrometry

  • Chang, Yoon-Seok;Jang, Jung-Suk
    • 분석과학
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    • 제8권4호
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    • pp.849-854
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    • 1995
  • A series of biologically active phosphopeptides were synthesized and their behavior in tandem mass spectrometry have been investigated. The structure identifications of other unusual peptides such as sulphated, glycosylated, lipoidal, and backbone modified peptides have been carried out. For all tested peptides, the structural modification could be determined directly by measurement of the absolute molecular weight in combination with collision-induced-dissociation in tandem mass spectrometry.

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Spatio-Temporal Variation of Cold Water Masses along the Eastern Coast of Korea in 2013 and 2014

  • Han, In-Seong;Park, Myung-Hee;Min, Seung-Hwan;Kim, Ju-Yeon
    • 해양환경안전학회지
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    • 제22권3호
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    • pp.286-295
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    • 2016
  • With the results of observations in 2013 and 2014 including ocean buoys, in-situ investigations and wind data, we examined the spatio-temporal variation of cold water masses along the eastern coast of Korea. Usually, a cold water mass first appears along the northern part of the eastern coast from May to July, and then along the southern part of the eastern coast from late June to mid-August. Cold water masses appear 3~5 times a year and remain for 5~20 days in the southwestern part of the East Sea. A distinctive cold water mass appeared usually in mid-July in this area, the surface temperature of which was below $10^{\circ}C$ in some cases. During the appearance of a cold water mass in the southwestern part of the East Sea, the horizontal temperature gradient was large at the surface and a significant low water temperature below $8^{\circ}C$ appeared at the bottom level. This appearance of cold water masses clearly corresponded to southwesterly winds, which generated coastal upwelling.

청년층 성인남성에서 흡연, 음주, 비만지표가 심뇌혈관계 위험인자에 미치는 영향 (Influencing Factors on Cardio-cerebrovascular Disease Risk Factors in Young Men: Focusing on Obesity Indices)

  • 김철규;이시향;차선경
    • Journal of Korean Biological Nursing Science
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    • 제19권1호
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    • pp.1-10
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    • 2017
  • Purpose: The purpose of this study was to investigate influences of drinking, smoking, and obesity indices on cardio-cerebrovascular disease risk factors in Korean young men. Methods: The subjects were 234 young men, aged 20 to 39 years. Body mass index (BMI), percent body fat (PBF), body fat mass (kg), and waist hip ratio (WHR) were measured as obesity indices. Systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood sugar (FBS), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured as cardio-cerebrovascular disease risk factors. The data were analyzed by t-test, ANOVA, Pearson's correlation coefficient, and multiple regression analysis using SAS 9.4. Results: Statistically significant factors were BMI for SEP; age, degree of drinking, body fat mass for DBP; WHR for TC; WHR and age for TG; age, degree of smoking and PBF for HDL-C; WHR, age and degree of smoking for LDL-C. Conclusion: Factors identified in this study need to be considered in cardio-cerebrovascular disease prevention programs for young men.

Constructing Proteome Reference Map of the Porcine Jejunal Cell Line (IPEC-J2) by Label-Free Mass Spectrometry

  • Kim, Sang Hoon;Pajarillo, Edward Alain B.;Balolong, Marilen P.;Lee, Ji Yoon;Kang, Dae-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제26권6호
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    • pp.1124-1131
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    • 2016
  • In this study, the global proteome of the IPEC-J2 cell line was evaluated using ultra-high performance liquid chromatography coupled to a quadrupole Q Exactive Orbitrap mass spectrometer. Proteins were isolated from highly confluent IPEC-J2 cells in biological replicates and analyzed by label-free mass spectrometry prior to matching against a porcine genomic dataset. The results identified 1,517 proteins, accounting for 7.35% of all genes in the porcine genome. The highly abundant proteins detected, such as actin, annexin A2, and AHNAK nucleoprotein, are involved in structural integrity, signaling mechanisms, and cellular homeostasis. The high abundance of heat shock proteins indicated their significance in cellular defenses, barrier function, and gut homeostasis. Pathway analysis and annotation using the Kyoto Encyclopedia of Genes and Genomes database resulted in a putative protein network map of the regulation of immunological responses and structural integrity in the cell line. The comprehensive proteome analysis of IPEC-J2 cells provides fundamental insights into overall protein expression and pathway dynamics that might be useful in cell adhesion studies and immunological applications.