• 한국미생물·생명공학회지
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• 제9권4호
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• pp.225-230
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• 1981

The kinetic study of rifamycin B production in batch culture of Nocardia mediterranei was undertaken in part of our endeavor to optimize the fermentation condition. The growth parameters such as $\mu$$_{m}$ and Ks values for nitrogen source were evaluated by employing Monod equation. From the experiments, $\mu$$_{m}$ and Ks were 0.15hr$^{-1}$ and 8.35g/1, respectively. The growth kinetics in batch culture was found successfully interpreted by logistic law, i.e., the initial specific growth rate and the maximum cell mass concentration were determined as function of pH and both found to have maxima. For the production of rifamycin B, a non-growth associated production kinetics was employed and the specific productivity as a function of pH was found to have two maximum points. The yield coefficient and the specific productivity were calculated as mean values in production phase. Utilizing these experimental data as a function of pH, the optimal condition for the rifamycin B production was discussed with regad to the pH effect on the cell growth and production of the antibiotic. As a result, growth phase at pH 6.5 and production phase at pH 7.0 were found to be recommended.ded.

• 품질경영학회지
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• 제42권2호
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• pp.179-187
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• 2014

Purpose: Since several problems were found when present non-conducting metal coating process was applied to mass production, we study and develop to improve those problems. Methods: In this paper, a couple of analysis methods such as surface hardness, XPS spectrum analysis, morphology, and reflection ratio were used. Results: This paper suggest a new possibility of Non-conducting thin metal coating method that has quality of mass production phase without UV coating process. Conclusion: By the result of analysis, we can set optimized process conditions of the electro deposition coating using electron beam.

• 융복합기술연구소 논문집
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• 제5권1호
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• pp.1-5
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• 2015

SI thermochemical hydrogen production process achieves water splitting into hydrogen and oxygen through three chemical reactions. The process is comprised of three sections and one of them is HI decomposition into $H_2$ and $I_2$ called as Section III. The production of $H_2$ included processes involving EED for concentrating a product stream from Section I. Additionally an $I_2$ crystallization would be considered to reduce burden on EED by removing certain amount of $I_2$ out of a process stream prior to EED. In this study, the current thermodynamic model of SI process was briefly described and the calculation results of the applied Electrolytes NRTL model for phase equilibrium calculations was illustrated for ternary systems of Section III. We calculated temperature and heat duty of an $I_2$ crystallizer and heat duty of heaters using UVa model and heat balance equation of simulation tool. The results were expected to be used as operation information in optimizing HI decomposition process and setting up material balance throughout SI process.

• International Journal of Precision Engineering and Manufacturing
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• 제7권2호
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• pp.36-40
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• 2006

Diagnosis and repair tasks of an unbalanced rigid rotor reduce the chances of unexpected failure and the consequent losses in production, time, and money. This paper presents investigation of a balancing system for equilibration of rigid rotor unbalance. A practical vibration signal based method is developed for unbalance diagnosis using wavelet technology and a Lissajous diagram. This paper shows that a mass unbalance can be efficiently estimated through an appropriate narrow-band filter used to extract the required spectra component. The wavelet technology is used to design specified narrow filter bank. A modified Lissajous diagram is also introduced with statistical analysis to compute the phase position. Several experimental tests demonstrate the effectiveness in balancing the mass unbalance of a rigid rotor.

• Animal cells and systems
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• 제3권1호
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• pp.29-39
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• 1999

Current data on reproductive characters of endemic and native species are essential to provide a strategy for the conservation of these species. Red pine (Pinus densiflora Sieb. & Zucc.) is one of the dominant, native tree species in Korea, but its reproductive ecology is not well-known. In 1997, the pattern of variation in cone and seed yields contributing to the conservation of declining populations of red pines was examined. Plant height and dbh were measured, and several new cones were collected from each tagged tree after counting the number of cones on each tree. For a subset of cones sampled, the number of fertile scales, the number of seeds at three development stages (early/late aborted, and filled seed), seed wing size, wing color, and individual filled seed mass were measured. The three sites which differed significantly in mean plant size also differed in mean cone and seed production per plant. However further analyses showed that most variation in characters examined occurred among plants within sites, but not among sites. An average of 90% of the potential seeds on the cones aborted at an early developmental stage, demonstrating that early abortion is a major factor affecting the number of filled seeds per cone. Individual seed mass was the only character which exhibited significant variations among sites as well as among trees within sites. Individual seed mass was overall negatively correlated with both the percentage of late abortion and the number of old cones per plant, suggesting that both the past and current years' reproductive activities have caused variations in seed mass. The potential dispersal distance of red pine seeds is quite large. However, wing loading was correlated with seed mass and number in a complex pattern across the sites. Distribution of seeds with varied colored wings differed among sites and among trees within sites. These results suggest that red pines at different sites might possess different strategies to cope with selection pressures acting during the final phase of reproduction, from seed dispersal to establishment. Then the ‘fitted’ red pine trees at each site should be identified and managed to conserve or restore populations.

• Animal Bioscience
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• 제37권4호
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• pp.689-696
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• 2024

Objective: Our study examined the impact of propriety blends of Bacillus strain probiotics on the performance, egg quality, and faecal microflora of laying hens. Methods: A total of 183 Institut de selection Animale (ISA) brown laying hens aged 23 weeks with an average body weight of 1,894±72 g were randomly allocated into 3 groups as control (corn-soybean meal based diet, CON), 0.5 g/kg Enterosure probiotics (ET1, 3×10⁸ colony-forming unit [CFU]/kg feed), and 5 g/kg Enterosure probiotics (ET2, 3×10⁹ CFU/kg feed) administered in mashed form. At the completion of each phase hen day egg production (HDEP), average egg weight (AEW), feed intake, and faecal microbiota were evaluated. Results: HDEP and AEW were higher (p<0.05) in the ET2-supplemented diet in phase 3 (week 9 to 12) compared with CON. Egg mass (EM) was higher (p<0.05) in phase 2 at ET2, and also higher (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets compared with CON. Feed conversion ratio was lower (p<0.05) in phase 3 at the ET1 and ET2-supplemented diets, with ET2 being the lowest compared with ET1 and CON. Yolk colour was higher (p<0.05) in the ET-supplemented diets at phase 3 compared with CON. Bifidobacterium spp. was higher (p<0.05) in the ET2- supplemented diet compared with CON in phase 2, while in In phase 3, Lactobacillus spp. and Bifidobacterium spp. were higher (p<0.05) in the ET-supplemented diets compared with CON. Coliforms were lower (p<0.05) in the ET-supplemented diets compared with CON in phase 3. Conclusion: The propriety blends of Bacillus strain probiotics supplements at 0.5 g/kg and 5 g/kg could improve the production and quality of eggs with more significance at 5 g/kg for HDEP, AEW and EM, which was achieved via the increase of beneficial microbiomes such as Lactobacillus spp., Bifidobacterium spp., and the decrease of pathogenic microbiomes like Escherichia coli and Coliforms which was speculated to improve gut barrier function and the reproductive hormone.

• Fisheries and Aquatic Sciences
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• 제11권4호
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• pp.224-228
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• 2008

With the aim of increasing the $CoQ_{10}$ production in mass culture, the effect of aeration-agitation on the $CoQ_{10}$ production using Rhodobactor sphaeroides was investigated in a l-L bioreactor. The maximum $CoQ_{10}$ production was 1.69 mg/g of dry cell weight under conditions of 50 Lux, $30^{\circ}C$, 300 rpm, and 5-vvm aeration. The $CoQ_{10}$ production was improved to produce 2.91 mg/g of dry cell weight under reduced conditions of agitation speed (200 rpm) and aeration rate (0.2 vvm). When R. sphaeroides was cultivated under more reduced DO levels during the exponential phase of the cell, the $CoQ_{10}$ production yield of 3.88-mg/g dry cell weight was the maximum obtained. Therefore, poorer conditions of aeration-agitation resulted in higher production of $CoQ_{10}$, and thus DO content was a crucial factor in the production of $CoQ_{10}$. Accordingly, it was necessary to control the DO concentration in order to enhance the $CoQ_{10}$ biosynthesis within a large-scale production.

• 한국산학기술학회논문지
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• 제12권6호
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• pp.2457-2462
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• 2011

생산방식의 변화에 따라 3차원 형상을 신속하고 정확하게 측정하는 것이 중요해졌다. 가장 훌륭한 비접촉 3차원 측정방법인 모아레 방법 중 잡음신호를 효과적으로 제거할 수 있는 위상천이 방법을 구현함에 있어 DMD를 이용하여 격자를 생성하고 이송하는 효과를 발생하도록 하였다. 컴퓨터에서 격자를 이송하고 위상천이를 발생하므로 기계적 이송으로 인한 잡음신호의 발생 가능성을 배제할 수 있으며 광학렌즈와의 적절한 조합을 통해 쉽게 분해능을 변화하며 3차원 정보 획득이 가능하다. 근본적인 $2\pi$ 모호성문제를 극복하기 위하여 2차원 위상정력을 실시하였다. 이 방법을 적용하여 3차원 형상의 측정을 수행하였다.

• Current Optics and Photonics
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• 제3권5호
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• pp.401-407
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• 2019

Polymeric optical waveguide devices with Bragg gratings have been investigated, for implementing tunable lasers and wavelength filters used in wavelength-division-multiplexed optical communication systems. Owing to the excellent thermo-optic effect of these polymers, wavelength tuning is possible over a wide range, which is difficult to achieve using other optical materials. In this study the phase-mask technology, which has advantages over the conventional interferometeric method, was introduced to facilitate the fabrication of Bragg gratings in polymeric optical waveguide devices. An optical setup capable of fabricating multiple Bragg gratings simultaneously on a 4-inch silicon wafer was constructed, using a 442-nm laser and phase mask. During fabrication, some of the diffracted light in the phase mask was totally reflected inside the mask, which affected the quality of the Bragg grating adversely, so experiments were conducted to solve this issue. To verify grating uniformity, two types of wavelength-filtering devices were fabricated using the phase-mask lithography, and their reflection and transmission spectra were measured. From the results, we confirmed that the phase-mask method provides good uniformity, and may be applied for mass production of polymer Bragg-grating waveguide devices.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.554-557
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• 2000

Strain JV3 was isolated from commercial Swiss cheese products and identified as a bacteriocin producer, which has bactericidal activity against Leuconostoc mesenteroides KCCM 11324. Strain JW3 was identified tentatively as Lactococcus lactis by the API test. The activity of lacticin JW3, named tentatively as the bacteriocin produced by Lactococcus lactis JW3, was detected during the mid-log growth phase, and reached a maximum during the early stationary phase, and decreased after the late stationary phase. Its antimicrobial activity on sensitive indicator cells was completely disappeared by protease IV. The inhibitory activities of lacticin JW3 were detected during treatments of up to $121\'^{circ}C$ for 15 min. Lacticin JW3 was very stable over a pH range of 2.0 to 9.0 The apparent molecular mass of lacticin JW3 was estimated to be in the region of 3-3.5kDa, which was determined by the direct detection of bactericidal activity after SDS-PAGE.