• Title/Summary/Keyword: Marker-set

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Screening of Rice Germplasm for the Distribution of Rice Blast Resistance Genes and Identification of Resistant Sources

  • Ali, Asjad;Hyun, Do-Yoon;Choi, Yu-Mi;Lee, Sukyeung;Oh, Sejong;Park, Hong-Jae;Lee, Myung-Chul
    • Korean Journal of Plant Resources
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    • v.29 no.6
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    • pp.658-669
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    • 2016
  • Rice blast, caused by a fungus Magnaporthe oryzae, is one of the most devastating diseases of rice worldwide. Analyzing the valuable genetic resources is important in making progress towards blast resistance. Molecular screening of major rice blast resistance (R) genes was determined in 2,509 accessions of rice germplasm from different geographic regions of Asia and Europe using PCR based markers which showed linkage to twelve major blast R genes, Pik-p, Pi39, Pit, Pik-m, Pi-d(t)2, Pii, Pib, Pik, Pita, Pita/Pita-2, Pi5, and Piz-t. Out of 2,509 accessions, only two accessions had maximum nine blast resistance genes followed by eighteen accessions each with eight R genes. The polygenic combination of three genes was possessed by maximum number of accessions (824), while among others 48 accessions possessed seven genes, 119 accessions had six genes, 267 accessions had five genes, 487 accessions had four genes, 646 accessions had two genes, and 98 accessions had single R gene. The Pik-p gene appeared to be omnipresent and was detected in all germplasm. Furthermore, principal component analysis (PCA) indicated that Pita, Pita/Pita-2, Pi-d(t)2, Pib and Pit were the major genes responsible for resistance in the germplasm. The present investigation revealed that a set of 68 elite germplasm accessions would have a competitive edge over the current resistance donors being utilized in the breeding programs. Overall, these results might be useful to identify and incorporate the resistance genes from germplasm into elite cultivars through marker assisted selection in rice breeding.

Development of Mandibular Movements Measuring System Using Double Stereo-Cameras

  • Park, Soon-Yong;Park, Sung-Kee;Cho, Chang-Hyun;Kim, Mun-Sang;Park, Mi-Gnon
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.1183-1188
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    • 2005
  • In this paper, we propose a 3D automated measuring system which measures the mandibular movements and the reference plane of the jaw movements. In diagnosis and treatment of the malocclusions, it is necessary to estimate the mandibular movements and the reference plane of the jaw movements. The proposed system is configured with double stereo-cameras, PC, two moving pattern plates(MPPs), two fixed pattern plates(FPPs) and one orbital marker. The virtual pattern plate is applied to calculate the homogeneous transformation matrices which describe the coordinates systems of the FPP and MPP with respect to the world coordinates system. To estimate the parameters of the hinge axis, the Euler's theorem is applied. The hinge axis points are intersections between the FPPs and the hinge axis. The coordinates of a hinge axis point with respect to the MPP coordinates system are set up to fixed value. And then, the paths of the jaw movement can be calculated by applying the homogeneous transformation matrix to fixed hinge axis point. To examine the accuracy of the measurements, experiments of measuring the hinge axis points and floating paths of them are performed using the jaw motion simulator. As results, the measurement errors of the hinge axis points are within reasonable boundary, and the floating paths are very similar to the simulator's moving path.

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Transcriptional Profiles of Peripheral Blood Leukocytes Identify Patients with Cholangiocarcinoma and Predict Outcome

  • Subimerb, Chutima;Wongkham, Chaisiri;Khuntikeo, Narong;Leelayuwat, Chanvit;McGrath, Michael S.;Wongkham, Sopit
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.10
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    • pp.4217-4224
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    • 2014
  • Cholangiocarcinoma (CCA), a slow growing but highly metastatic tumor, is highly prevalent in Northeast Thailand. Specific tests that predict prognosis of CCA remain elusive. The present study was designed to investigate whether peripheral blood leukocyte (PBL) transcriptional profiles might be of use as a prognostic test in CCA patients. Gene expression profiles of PBLs from 9 CCA and 8 healthy subjects were conducted using the Affymetrix HG_U133 Plus 2.0 GeneChip. We indentified informative PBLs gene expression profiles that could reliably distinguish CCA patients from healthy subjects. Of these CCA specific genes, 117 genes were up regulated and 60 were down regulated. The molecular and cellular functions predicted for these CCA specific genes according to the Gene Ontology database indicated differential PBL expression of host immune response and tumor progression genes (EREG, TGF ${\beta}1$, CXCL2, CXCL3, IL-8, and VEGFA). The expression levels of 9 differentially expressed genes were verified in 36 CCA vs 20 healthy subjects. A set of three tumor invasion related genes (PLAU, CTSL and SERPINB2) computed as "prognostic index" was found to be an independent and statistically significant predictor for CCA patient survival. The present study shows that CCA PBLs may serve as disease predictive clinically accessible surrogates for indentifying expressed genes reflective of CCA disease severity.

Mapping QTL for ratooning ability in advanced backcross lines from an Oryza sativa × O. rufipogon cross

  • Ji, Shi-Dong;Luo, Xiao;Ahn, Sang-Nag
    • Korean Journal of Agricultural Science
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    • v.41 no.1
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    • pp.1-7
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    • 2014
  • Ratooning ability is one of the major different traits from perennial to cultivated rice and annual type. We developed a set of 126 introgression lines derived from a cross between Hwayeong and W1944 (O. rufipogon) to gain an insight into the genetic factors underlying differences between common wild rice and cultivated rice. One IL, CR6 among the 126 ILs of $BC_3F_4$ showed a significant difference in rationing ability compared with Hwayeong. To further characterize the rationing ability, CR6 was selected and crossed to Hwayeong to produce three secondary populations, $BC_4F_2$, $BC_4F_3$ and $BC_5F_2$. In the Hwayeong background, the W1944 allele was associated with an increase in rationing ability. QTL analysis showed that the qRAT5 for rationing ability was linked to RM194 ($R^2$=6.6%, 19.6%, and 44.5% in the $BC_4F_2$, $BC_5F_2$, and $BC_5F_3$, respectively). The putative qRAT5 was also tightly linked to QTLs for spikelets per panicle and grain weight indicating that this region harbors a QTL cluster related to domestication. To our knowledge, this is the first report to map the major QTL for ratooning ability in rice. The SSR markers linked to qRAT5 would be useful in marker-assisted selection for breeding lines with enhanced ratooning ability.

A Multibit Tree Bitmap based Packet Classification (멀티 비트 트리 비트맵 기반 패킷 분류)

  • 최병철;이정태
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.3B
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    • pp.339-348
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    • 2004
  • Packet classification is an important factor to support various services such as QoS guarantee and VPN for users in Internet. Packet classification is a searching process for best matching rule on rule tables by employing multi-field such as source address, protocol, and port number as well as destination address in If header. In this paper, we propose hardware based packet classification algorithm by employing tree bitmap of multi-bit trio. We divided prefixes of searching fields and rule into multi-bit stride, and perform a rule searching with multi-bit of fixed size. The proposed scheme can reduce the access times taking for rule search by employing indexing key in a fixed size of upper bits of rule prefixes. We also employ a marker prefixes in order to remove backtracking during searching a rule. In this paper, we generate two dimensional random rule set of source address and destination address using routing tables provided by IPMA Project, and compare its memory usages and performance.

Development of Low Altitude Terrain Following System based on TERain PROfile Matching (TERPROM 기반의 저고도 지형추적시스템 개발)

  • Kim, Chong-sup;Cho, In-je;Lee, Dong-Kyu;Kang, Im-Ju
    • Journal of Institute of Control, Robotics and Systems
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    • v.21 no.9
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    • pp.888-897
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    • 2015
  • A flight capability to take a terrain following flight near the ground is required to reduce the probability that a fighter aircraft can be detected by foe's radar fence in the battlefield. The success rate for mission flight has increased by adopting TFS (Terrain Following System) to enable the modern advanced fighter to fly safely near the ground at the low altitude. This system has applied to the state-of-the-art fighter and bomber, such as B-1, F-111, F-16 E/F and F-15, since the research begins from 1960's. In this paper, the terrain following system and GCAS (Ground Collision Avoidance System) was developed, based on a digital database with UTAS's TERPRROM (TERrain PROfile Matching) equipment. This system calculates the relative location of the aircraft in the terrain database by using the aircraft status information provided by the radar altimeter and the INS (Inertial Navigation System), based on the digital terrain database loaded previously in the DTC (Data Transfer Cartridge), and figures out terrain features around. And, the system is a manual terrain following system which makes a steering command cue refer to flight path marker, on the HUD (Head Up Display), for vertical acceleration essential for terrain following flight and enables a pilot to follow it. The cue is based on the recognized terrain features and TCH (Target Clearance Height) set by a pilot in advance. The developed terrain following system was verified in the real-time pilot evaluation in FA-50 HQS (Handling Quality Simulator) environment.

Design and Implementation of Frontal-View Algorithm for Smartphone Gyroscopes (스마트폰 자이로센서를 이용한 Frontal-View 변환 알고리즘 설계 및 구현)

  • Cho, Dae-Kyun;Park, Seok-Cheon
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.6
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    • pp.199-206
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    • 2012
  • Attempt to use as a marker of natural objects directly in the real world, but there is a way to use the accelerometer of the smartphone, to convert the Frontal-View virtual, because it asks only the pitch of the camera, from the side there is a drawback that can not be converted to images. The proposed algorithm, to obtain the rotation matrix of axis 3 pitch, roll, yaw, we set the reference point of the yaw of the target image. Then, to compensate for the rotation matrix to determine Myon'inji any floor, wall, the ceiling of the target image. Finally, to obtain the homography matrix for obtaining the Frontal-View to account for the difference between the gyro sensor coordinate system and image coordinate system, so we can get the Frontal-View from the captured images through the projection transformation was designed. Was tested to convert Frontal-View the picture was taken in an environment smartphone environment surrounding floor, walls and ceiling in order to evaluate the conversion program Frontal-View has been implemented, in this paper, design and The conversion algorithm implementation, it was confirmed that to convert a regular basis Frontal-View footage taken from multiple angles.

Development of AFLP and STS Markers Related to Stay Green Trait in Multi-Tillered Maize

  • Jang Cheol Seong;Lee Hee Bong;Seo Yong Weon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.4
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    • pp.358-362
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    • 2004
  • In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a $91\%$ homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a $96\%$ homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.

Phenotypic and genotypic screening of rice accessions for salt tolerance

  • Reddy, Inja Naga Bheema Lingeswar;Kim, Sung-Mi;Yoon, In Sun;Kim, Beom-Gi;Kwon, Taek-Ryoun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.188-188
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    • 2017
  • Rice (Oryza sativa L.) is one of the major crops that is seriously impacted by global soil salinization. Rice is among those crops where most of the high-yielding cultivars are highly sensitive to salinity. The key to a plant survival under NaCl salt stress is by maintaining a high $K^+/Na^+$ ratio in its cells. Selection for salinity tolerance genotypes of rice based on phenotypic performance alone is less reliable and will delay in progress in breeding. Recent advent of molecular markers, microsatellites or simple sequence repeats (SSRs) were used to find out salt tolerant rice genotypes. In the current experiment phenotyping and genotyping studies were correlated to differentiate different rice accessions for salinity tolerance. Eight rice accessions along with check plant Dongjin were screened by physiological studies using Yoshida solution with 50mM NaCl stress condition. The physiology studies identified four tolerant and four susceptible accessions based on their potassium concentration, sodium concentration, $K^+/Na^+$ ratio and biomass. 17 SSR markers were used to evaluate these rice accessions for salt tolerance out of which five molecular markers were able to discriminate tolerant accessions from the susceptible accessions. Banding pattern of the accessions was scored comparing to the banding pattern of Dongjin. The study identifies accessions based on their association of $K^+/Na^+$ ratio with molecular markers which is very reliable. These markers identified can play a significant role in screening large set of rice accessions for salt tolerance; these markers can be utilized to improve salt tolerance of commercial rice varieties with marker-assisted selection (MAS) approach.

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Contrasting rice sub-populations to tocols ratio associated with seed longevity

  • Lee, Jae-Sung;Kwak, Jieun;Yoon, Mi-Ra;Lee, Jeom-Sig;Hay, Fiona R.
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.31-31
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    • 2017
  • Understanding the mechanism(s) to overcome or prevent seed ageing deterioration during storage is of fundamental interest to seed physiologists. Vitamin E (tocols) is known as a key metabolite to efficiently scavenge lipid peroxy radicals which cause membrane breakdown resulting in seed ageing. However, in rice research this hypothesis has been tested for very few lines only without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing aus, indica, temperate japonica and tropical japonica subpopulations, were used for tocols analysis (quantification of ${\alpha}$-, ${\beta}$-, ${\gamma}$-, ${\delta}$-tocopherol/tocotrienol by ultra performance liquid chromatography) and storage experiments at $45^{\circ}C$ and 10.9% seed moisture content (sample taken for germination testing every 3 days up to 60 days). To examine interactions between DNA sequences and phenotype, the 700k high-density single-nucleotide polymorphism marker data-set was utilized. Both seed longevity (time for viability to fall to 50%; $p_{50}$) and tocols content varied across subpopulations due to heterogeneity in the genetic architecture. Among eight types of tocol homologues, ${\alpha}$-tocopherol and ${\gamma}$-tocotrienol were significantly correlated with $p_{50}$ (negatively and positively, respectively). While temperate japonica varieties were most abundant in ${\alpha}$-tocopherol, indica varieties recorded 1.3 to 1.7-fold higher ${\gamma}$-tocotrienol than those of other subpopulations. It was highlighted that specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

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