• Journal of the Korea Society of Computer and Information
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• v.21 no.11
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• pp.9-16
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• 2016

The central goal of the current paper is to investigate lexical transfer between Korean and English and to identify rule-governed behavior and to provide implications for development of computer-assisted translation(CAT) software for the two languages. It will be shown that Sankoff and Poplack's Free Morpheme Constraint can not account for all the range of data. A constraint is proposed that a set of case-assigners such as verbs, INFL, prepositions, and the possessive marker may not undergo lexical transfer. The translation software is also expected to be equipped with the proposed claim that English verbs are actually borrowed as nouns or as defective verbs to escape from the direct attachment of inflectional morphemes.

• ETRI Journal
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• v.33 no.2
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• pp.259-266
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• 2011

We present a new method for an automated markerless system to describe, analyze, and classify human gait motion. The automated system consists of three stages: I) detection and extraction of the moving human body and its contour from image sequences, ii) extraction of gait figures by the joint angles and body points, and iii) analysis of motion parameters and feature extraction for classifying human gait. A sequential set of 2D stick figures is used to represent the human gait motion, and the features based on motion parameters are determined from the sequence of extracted gait figures. Then, a k-nearest neighbor classifier is used to classify the gait patterns. In experiments, this provides an alternative estimate of biomechanical parameters on a large population of subjects, suggesting that the estimate of variance by marker-based techniques appeared generous. This is a very effective and well-defined representation method for analyzing the gait motion. As such, the markerless approach confirms uniqueness of the gait as earlier studies and encourages further development along these lines.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.467-477
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• 2018

Colletotrichum circinans, an anthracnose pathogen, causes serious damage to onions worldwide. In this study, specific molecular markers were developed to detect C. circinans accurately and quickly with both conventional and real-time PCR methods. The cirTef-F/cirTef-R and cirTu-F/cirTu-R primer sets, which are specific for C. circinans, were constructed by analyzing $tef-1{\alpha}$ and ${\beta}-tubulin$ genes in the fungus. Using the conventional PCR method, 100 pg and 1 ng of fungal DNA could be detected using the cirTef-F/cirTef-R and cirTu-F/cirTu-R sets, respectively. Using the real-time PCR method, 10 pg and 100 pg of fungal DNA could be detected more sensitively with the cirTef-F/cirTef-R and cirTu-F/cirTu-R sets, respectively. Detection of C. circinans from the artificially infected onion seeds was possible by using both conventional and real-time PCR methods and the developed cirTef-F/cirTef-R primer set. The PCR markers specific for C. circinans developed in this study may enhance the efficiency of fungal pathogen detection in imported vegetables and seeds.

• Genomics & Informatics
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• v.14 no.4
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• pp.196-204
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• 2016

Many researchers have found that one of the most important characteristics of the structure of linkage disequilibrium is that the human genome can be divided into non-overlapping block partitions in which only a small number of haplotypes are observed. The location and distribution of haplotype blocks can be seen as a population property influenced by population genetic events such as selection, mutation, recombination and population structure. In this study, we investigate the effects of the density of markers relative to the full set of all polymorphisms in the region on the results of haplotype partitioning for five popular haplotype block partition methods: three methods in Haploview (confidence interval, four gamete test, and solid spine), MIG++ implemented in PLINK 1.9 and S-MIG++. We used several experimental datasets obtained by sampling subsets of single nucleotide polymorphism (SNP) markers of chromosome 22 region in the 1000 Genomes Project data and also the HapMap phase 3 data to compare the results of haplotype block partitions by five methods. With decreasing sampling ratio down to 20% of the original SNP markers, the total number of haplotype blocks decreases and the length of haplotype blocks increases for all algorithms. When we examined the marker-independence of the haplotype block locations constructed from the datasets of different density, the results using below 50% of the entire SNP markers were very different from the results using the entire SNP markers. We conclude that the haplotype block construction results should be used and interpreted carefully depending on the selection of markers and the purpose of the study.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.4
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• pp.227-231
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• 2000

Small seed size is one of the major traits of soybean cultivars for sprouts with regard to high sprout yield. This study was conducted to identify quantitative trait loci (QTL) for seed size and weight in a set of F 6 seeds of 89 lines derived from a cross between 'Pureunkong', a soybean cultivar developed for sprouts and 'Jinpumkong 2', a soybean cultivar with no beany taste in seed due to the lack of lipoxygenases. The genetic map of 25 linkage groups with a total of 98 markers including RFLP, RAPD, SSR and classical markers was constructed from this F/sbu 5/-derived population and was used for QTL analysis. 'Pureunkong' was significantly smaller (P＜0.01) than 'Jinpumkong 2' in seed size and seed weight. Genetic variation was detected and transgressive segregation was common in the population for these traits. Seven DNA markers including opT14-1600 in LG A2, opF02-400 in LG B2, Satt100, opC09-700, opG04-730 and opQll-650 in LG C2, and opY07-1100 ＆ 1000 in LG(unknown) were significantly associated and accounted for 4.7 to 10.9% and 5.1 to 10.1 % of the phenotypic variation in seed size and seed weight, respectively. 'Pureunkong' alleles increased seed size and seed weight at the all four significant marker loci on the LG C2. These marker loci in LG C2 were closely linked and were presumed to be a single QTL. Overall, at least three independent QTLs from 3 linkage groups (A2, B2, and C2) were putatively involved in the control of seed size and seed weight.

• Korean Journal of Pharmacognosy
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• v.40 no.4
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• pp.298-302
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• 2009

Samultang is one of traditional medicine composed of Paeonia lactiflora, Angelica gigas, Rehmannia glutinosa and Cnidium officinale. To develop simultaneous determination of paeoniflorin, decursin and 5-HMF in Samultang, a high performance liquid chromatography with diode array detector was used. To separate three marker components, Dionex $C_{18}$ column (5 ${\mu}m$, 120 ${\AA}$, 4.6 mm${\times}$150 mm) was used with a gradient elution system of water and methanol. UV wavelength of detector set at 230 nm and 280 nm. This method was validated by linearity, precision test and recovery test. Calibration curves of three standard components were showed good linear regression ($R^2$>0.9973). LOD and LOQ ranged from 0.08 ${\mu}g$/ml to 0.38 ${\mu}g$/ml and 0.25 ${\mu}g$/ml to 1.16 ${\mu}g$/ml, respectively. The relative standard deviations (RSDs) of data of the inter-day and intra-day experiments were less than 0.54% and 0.89%, respectively. The measured results of recovery test were varied from 93.36 to 107.79 with RSD values 0.01~1.45%. The established method was applied for separation of bio-conversion Samultang sample and compared with control sample.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.7
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• pp.936-942
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• 2015

The purpose of this study was to characterize genetic architecture of behavior patterns in Sapsaree dogs. The breed population (n=8,256) has been constructed since 1990 over 12 generations and managed at the Sapsaree Breeding Research Institute, Gyeongsan, Korea. Seven behavioral traits were investigated for 882 individuals. The traits were classified as a quantitative or a categorical group, and heritabilities ($h^2$) and variance components were estimated under the Animal model using ASREML 2.0 software program. In general, the $h^2$ estimates of the traits ranged between 0.00 and 0.16. Strong genetic ($r_G$) and phenotypic ($r_P$) correlations were observed between nerve stability, affability and adaptability, i.e. 0.9 to 0.94 and 0.46 to 0.68, respectively. To detect significant single nucleotide polymorphism (SNP) for the behavioral traits, a total of 134 and 60 samples were genotyped using the Illumina 22K CanineSNP20 and 170K CanineHD bead chips, respectively. Two datasets comprising 60 (Sap60) and 183 (Sap183) samples were analyzed, respectively, of which the latter was based on the SNPs that were embedded on both the 22K and 170K chips. To perform genome-wide association analysis, each SNP was considered with the residuals of each phenotype that were adjusted for sex and year of birth as fixed effects. A least squares based single marker regression analysis was followed by a stepwise regression procedure for the significant SNPs (p<0.01), to determine a best set of SNPs for each trait. A total of 41 SNPs were detected with the Sap183 samples for the behavior traits. The significant SNPs need to be verified using other samples, so as to be utilized to improve behavior traits via marker-assisted selection in the Sapsaree population.

• Journal of the Society of Naval Architects of Korea
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• v.51 no.5
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• pp.419-428
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• 2014

This paper presents a numerical simulation method for the flow around advancing ships in regular waves by using a rectilinear grid system. Because the grid lines do not consist with body surface in the rectilinear grid system, the body geometries are defined by the interaction points of those grid lines and the body surface. For the satisfaction of body boundary conditions, no-slip and divergence free conditions are imposed on the body surface and body boundary cells, respectively. Meanwhile, free surface is defined with the modified marker density method. The pressure on the free surface is determined to make the pressure gradient terms of the governing equations continuous, and the velocity around the free surface is calculated with the pressure on the free surface. To validate the present numerical method, a vortex induced vibration (VIV) phenomenon and flows around an advancing Wigley III ship model in various regular waves are simulated, and the results are compared with existing and corresponding research data. Also, to check the applicability to practical ship model, flows around KRISO Container Ship (KCS) model advancing in calm water are numerically simulated. On the simulations, the trim and the sinkage are set free to compare the running attitude with some other experimental data. Moreover, flows around the KCS model in regular waves are also simulated.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.3
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• pp.747-752
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• 2011

Microarray classification typically possesses two striking attributes: (1) classifier design and error estimation are based on remarkably small samples and (2) cross-validation error estimation is employed in the majority of the papers. A Microarray data of ovarian cancer consists of the expressions of thens of thousands of genes, and there is no systematic procedure to analyze this information instantaneously. In this paper, gene markers are selected by ranking genes according to statistics, popular classification rules - linear discriminant analysis, k-nearest-neighbor and decision trees - has been performed comparing classification accuracy of data selecting gene markers and not selecting gene markers. The Result that apply linear classification analysis at Microarray data set including marker gene that are selected using ANOVA method represent the highest classification accuracy of 97.78% and the lowest prediction error estimate.

• Journal of Microbiology and Biotechnology
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• v.17 no.5
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• pp.806-811
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• 2007

Bacillus anthracis is a soil pathogen capable of causing anthrax that is closely related to several environmental species, including B. cereus, B. mycoides, and B. thuringiensis. DNA homology studies showed that B. anthracis, B. cereus, B. mycoides, and B. thuringiensis are closely related, with a high sequence homology. To establish a method to specifically detect B. anthracis in situations such as environmental contamination, we initially performed RAPD-PCR with a 10-mer random primer and confirmed the presence of specific PCR bands only in B. anthracis species. One region specific for B. anthracis was cloned and sequenced, and an internal primer set was designed to amplify a 241-bp DNA fragment within the sequenced region. The PCR system involving these specific primer sets has practical applications. Using lyses methods to prepare the samples for PCR, it was possible to quickly amplify the 241-bp DNA segment from samples containing only a few bacteria. Thus, the PCR detection method developed in this study is expected to facilitate the monitoring of environmental B. anthracis contamination.