• Journal of Marine Bioscience and Biotechnology
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• v.15 no.2
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• pp.49-58
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• 2023

Early-stage lung cancer is the deadliest form of the disease. In this study, we investigated the anticancer activity of 5-bromoprotocatechualdehyde (BPCA) extracted from the seaweed Polysiphonia morrowii Harvey (P. morrowii) in lung cancer H460 cells. We extracted P. morrowii powder thrice with 80% aqueous methanol and separated the extract using high-performance liquid chromatography. We then tested BPCA's effects on cell viability, apoptosis, reactive oxygen species (ROS) generation, and protein expression Our results showed that BPCA inhibited tumor cell growth and ROS production and induced apoptosis through mitogen-activated protein kinase (MAPK) and AKT signaling pathways in lung cancer cells. When BPCA was combined with hydrogen peroxide, ROS production and apoptosis increased even further due to the regulation of AKT signaling and JNK-MAPKs pathways. These findings suggest that BPCA induces lung-cancer-cell death through ROS-mediated phosphorylation in AKT/MAPK signaling. This could lead to the development of new and effective treatments for early-stage lung cancer.

• ALGAE
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• v.31 no.3
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• pp.277-287
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• 2016

Five fractions separated from Nannochloropsis oculata using solvent-solvent partition chromatography of 80% methanolic extract of N. oculata (NOM) followed by the open silica column chromatography of its hexane fraction (NOMH) for the anti-inflammatory on RAW 264.7 cells and anti-cancer activities on HL-60, A-549, HEP-3B, HCT-116, and SW-480 cancer cells. All the five fractions showed potential anti-inflammatory activities against lipopolysaccharide-stimulated RAW 264.7 macrophages cells with IC₅₀ values less than 6.25 μg mL⁻¹. Moreover, 90% n-hexane column elution of NOMH (NOMH90) down-regulated lipopolysaccharide-stimulated protein levels of inducible nitric oxide synthase and cyclooxygenase-2. Furthermore, NOMH90 showed marked cytotoxic effect on the HL-60 cells with IC₅₀ value of 23.58 ± 0.09 μg mL⁻¹. In addition, Hoechst 33342 cell permeable dye used to visualize the apoptosis nucleus and cell cycle analysis measured Sub-G1 DNA contents to confirm reduction of the cell viability in NOMH90 treated cells due to induction of apoptosis in HL60. These results are quite related to the phytosterol contents of the NOMH fractions and the results suggest N. oculata extracts might be useful as potential sources of natural anti-inflammatory and anti-cancer compounds. In conclusion, the sterol content in N. oculata might provide a promising role in future medicines in anti-inflammatory and anti-cancer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.4
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• pp.365-369
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• 2001

Streptococcus spp. of bacterial pathogen of fish were isolated from the cultured flounder (Paralichthys olivaceus) in fish farm of Jeju Island. Clinical signs of the infected flounder which are the most commons symptoms are as follows: erratic swimming, darkening of the body colour, unilateral or bilateral exophthalmia, corneal opacity, hemorrhages in the opercular and the bases of the fins, and the ulceration of the body surface. Biochemical characteristics of pathogenic fish Streptococcus spp, were gram positive, spherical form, catalase negative, oxidase negative and $\beta$-haemolytic, respectively, The viable cells counted from the tissue of the diseased flounder were the largest in the order of the ulcer, the kidney, the blood and the brain, The drugs used were ampicillin, ciprofloxacin, doxycycline, gentamycin, tetracycline, erythromycin, streptomycin and oxytetracycline, Streptococcus spp. were found to be sensitive to ampicillin, ciprofloxacin, doxycycline and gentamycin, but were resistant to tetracycline, erythromycin, streptomycin and oxytetracycline. The pathogenicity of Streptococcus spp. on the cultured flounder with an abdominal cavity injection was high. The haemolytic activity of the toxin against the sheep red blood cells reached the maximum after 30 min incubation at $37^{\circ}C$ or $50^{\circ}C$. The toxin showed highest activity at pH $5.5\sim6.5$.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.4
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• pp.913-924
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• 2016

Re-consultation on Marine Environment Impact Assessment (MEIA) says that business operators are then licensed back from the disposition authorities undergoing a consultation process to change business plans. Marine Environment Management Act has provisions(Article 94) with respect to a case where the scale of business, the period of business, reflects the basic plan, including a plan to change a variety of reasons. But increasingly diverse types of businesses and projects which are the subject of MEIA, As it appears that the vulnerability issues and the problem of the re-consultation. In this study conducted an analysis of the literature review and comment on the re-consultation report four years. In addition, it presents a check to the problem through concrete examples related to the re-consultation and proposes improvement measures.

• Proceedings of the Korean Society of Life Science Conference
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• 2003.10a
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• pp.122-122
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• 2003

• International Journal of Naval Architecture and Ocean Engineering
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• v.11 no.2
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• pp.875-882
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• 2019

The effects of Triangle Groove Strips (TGS) on Vortex-induced Vibration (VIV) suppression of marine riser are numerically investigated using Computational Fluid Dynamics (CFD) method. The range of Reynolds number in simulations is 4.0 × 10⁴ < Re < 1.2 × 10⁵. The two-dimensional unsteady Reynolds-Averaged Navier-Stokes (RANS) equations and Shear Stress Transport (SST) k-ω turbulence model are used to calculate the flow around marine riser. The Newmark-β method is employed for evaluating the structure dynamics of marine riser. The effect of the height ratio (ε) of TGS on VIV suppression is evaluated. The amplitude responses, frequency responses, vortex patterns and the flow around the structures are discussed in detail. With the increase of the height ratio of TGS, the suppression effect of TGS on VIV suppression is improved firstly and then weakened. When ε=0.04, the suppression effect of TGS is the best. Compared with the VIV responses of smooth marine riser, the amplitude ratio is reduced by 38.9%, the peak of the lift coefficient is reduced by 69% and the peak of the drag coefficient is reduced by 40% when Re=6.0 × 10⁴. With the increase of Reynolds number, the suppression effect of TGS on VIV suppression is improved firstly and then weakened. When the Reynolds number is 7.0 × 10⁴, the amplitude ratio can be reduced by 40.1%. As to the large-amplitude vibration cases, the TGS show nice suppression effect on VIV.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.94-101
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• 2022

In this study, the marine medaka Oryzias javanicus was exposed to two concentrations of non-toxin-producing red tide dinoflagellate C. polykrikoides (1,000 and 2,000 cells ml^-1) for 96 h, and the time-course biochemical responses of antioxidant and immunity parameters were analyzed in the liver tissue. Significant ichthyotoxicity with increasing cell concentrations of C. polykrikoides and exposure period was observed for 96 h. Opercular respiratory rate was lowered in marine medaka exposed to 2,000 cells ml^-1 of C. polykrikoides. Intracellular malondialdehyde (MDA) content significantly elevated in response to both cell concentrations. In the case of glutathione (GSH) content, the levels were significantly elevated by 1,000 cells ml^-1 of C. polykrikoides, but the contents significantly depleted upon exposure to 2,000 cells ml^-1 of C. polykrikoides. Similarly, enzymatic activities of catalase (CAT) and superoxide dismutase (SOD) were increased by 1,000 cells ml^-1 of C. polykrikoides, whereas their activities were lowered by 2,000 cells ml^-1 of C. polykrikoides. Analysis of the two immunity parameters, alternative complement pathway and lysozyme, showed significantly lowered activities in 2,000 cells ml^-1 of C. polykrikoides-exposed liver tissue. These biochemical effects of C. polykrikoides on marine medaka would be helpful for understanding its acute effects in marine fish.

• Food Science and Biotechnology
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• v.17 no.5
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• pp.976-982
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• 2008

The 10 species of marine green algae was collected from Jejudo(Island) in Korea. Methanolic and aqueous extracts were prepared and screened for inhibition activities against tumor cell growth. Of the tested samples, the sea staghorn (Codium fragile) aqueous extract (CFAE) showed the highest activity on CT-26 cell growth. Therefore, CFAE was selected for further experiments and the possibility to induce apoptosis by the CFAE was investigated. Flow cytometric analysis revealed that it dose-dependently increased apoptotic cells with hypodiploid DNA contents in CT-26 cell line. These results indicated that CFAE can suppress the growth of CT-26 cells through apoptosis. The CFAE decreased the protein expression of anti-apoptotic Bcl-xL and led to the activation of caspase-3 and -7. A crude polysaccharide was separated from CFAE and it mainly constituted with 61.2% galactose and 30.5% arabinose as analyzed by high performance liquid chromatography (HPLC).

• Ocean Science Journal
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• v.43 no.2
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• pp.115-126
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• 2008

Direct measurements of four radiative components at air-sea boundary layer were conducted in the southern Yellow Sea during three cruises (seasons) in 2007. Simultaneous observations of meteorological (cloud cover, air temperature and humidity) and oceanographic (sea surface temperature) parameters were carried out. Observational results of radiative fluxes and meteorological and oceanographic parameters are presented. Mean diurnal cycles of four radiative components, net radiation, and sea surface albedo are calculated to achieve averages in different seasons. Net radiative fluxes in three seasons (winter, spring, autumn) are 8, 146, $60\;W/m^2$, respectively. Comparisons between the observed radiative fluxes and those estimated with formulas are taken.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.3
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• pp.456-463
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• 2020

The diet of the walleye pollock Gadus chalcogrammus, was studied by examining the stomach contents of 864 individuals. Using gill nets, samples were collected monthly from January to December 2017 in Jugwang, Sokcho, Yangyang, Uljin, Ganggu, and Gampo, Korea. The specimens ranged in total length (TL) from 16.6 to 75.2 cm. G. chalcogrammus fed mainly on Euphausia pacifica (Euphausiacea) and less on Cephalopoda, Pisces, Mysidacea, and Macrura. The results indicated that G. chalcogrammus is a specialist feeder, feeding mainly Euphausiacea. With growth, however, its diet shifted from Euphausiacea to Cephalopoda, Macrura and Pisces. Smaller individuals (<40.0 cm TL) consumed mainly Euphausiacea. The proportion of Euphausiacea decreased with increasing body size, whereas the consumption of Cephalopoda and Macrura increased gradually.