• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.109-117
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• 1986

For the purpose of obtaining basic data which can be applied to processing of retort pouched shellfishes, retort pouched seasoned ark shell, Anadara broughtonii, was prepared. The frozen ark shell was thawed and seasoned with a mixed seasoning powder prepared with $10.0\%$ of sorbitol, $2.0\%$ of table salt and $0.5\%$ of monosodium glutamate at $5^{\circ}C$ for 10 hours, and then dried at $45^{\circ}C$ for 4 hours. The dried seasoned ark shell was coated with $1.0\%$ sodium alginate solution, dried with cola air blast for 2 hours and then vacuum-packed in the laminated plastic film bag (polyester/casted polypropylene= $12{\mu}m/70{\mu}m,\;15{\times}16cm$), and finally sterilized up to Fo=6.0 in hot water circulating retort at $121^{\circ}C$ for 10 minutes. The major fatty acids of raw ark shell and retort pouched seasoned ark shell products were 16:0, 20:5, 22:6, 18:0 and 18:3, and predominant free amino acids of those were lysine, arginine, glycine, alanine, glutamic acid and leucine. In nucleotides and its related compounds of raw ark shell and retort pouched seasoned ark shell products, the most abundant one was AMP, and total extract-N of those was chiefly consisted of free amino acids, betaine and nucleotide and its related compounds. During the processing procedure such as drying and sterilization, unsaturated fatty acids slightly decreased while saturated fatty acids increased, and total extract-N content decreased about a half. From the results of chemical and microbial experiments during storage, it was concluded that the products could be preserved in a good condition for 100 days at room temperature, and their duality could be improved by the coating treatment of sodium alginate solution.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.6
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• pp.550-556
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• 1985

In order to evaluate the marine mollusc muscle as foodstuff not only from the biochemical aspect but also from the view point of food science, we have analyzed the protein and amino acid compositions of the echiurid (Urechis unicinctus) and sea hare (Aplysia kurodai) muscle. The protein quality of the muscles was also investigated using in vitro methods based on in vitro digestibility, predicted digestibility, computed PER (C-PER) and discriminant computed PER(DC-PER). The remarkable feature of the protein compositions of the both muscles was that water soluble protein occupied a large amount of the muscle protein with fairly lower contents of the salt soluble protein. From the analysis of SDS-PAG electrophoresis, the sarcoplasmic proteins in the echiurid and the sea hare muscles were composed of 15 and 10 subunits, respectively. The free amino acid compositions of the total amino acids in the echiurid and sea hare muscle were characterized with $75\%$ of glycine and alanine, and with $78\%$ of taurine, respectively. The amino acid anaylsis of both muscle proteins showed that the echiurid muscle was rich in glycine, aspartic acid, glutamic acid, arginine and lysine, but was poor in cysteine, while the sea hare muscle was rich in glycine, glutamic acid, aspartic acid and arginine, but was negligible in cysteine and tryptophan. In the total amino acid profiles of the freeze dried muscles in echiurid and sea hare, there was not found a significant difference compared to the amino acid compositions of the muscle proteins. Predicting the protein quality of the echiurid and sea hare muscles using the in vitro method, it was apparently low compared to the muscle protein of fishes.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.45-54
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• 2012

We investigated environmental characteristics of the tidal flat oyster aquafarms to clarifyeffects of the tidal flat environmental factors on growth and mortality of the tidal flat oyster Crassostrea gigas by the Net Bag Rack Culture System in two districts in Western Korea. In this study, we have carried out the basic environmental investigation on growth of the single tidal flat, water quality and sediments, etc. in Taean and Seosan districts, Choongcheongnam-do, where the single tidal flat oyster aquaculture have been performed by the net bag rack culture system. In June 2011 when the final survey carried out at the two districts in western Korea, the mortality in Seosan district was higher than that in Tae-an district. Judging from the results of growth and mortality of the single tidal flat oysters investigated at two sites of Jinsan-ri in Taean and Chang-ri in Seosan districts, two results of Taean district showed higher growth and lower mortality than those of Seosan district. It is assumed that the proper acceptable density possible for growth of the tidal flat seeds and the secure of economics, in case of plastic cultivate net with the size of $50{\times}80cm$, are about 200 individuals. In this study, It was clarified that selection of the suitable sites, the input into the net bag of high quality oyster seeds and selective dispersion in the optimal density of the single tidal flat oyster have an effect on growth and mortality of the tidal flat oyster.

• Korean Journal of Environmental Biology
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• v.18 no.1
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• pp.77-93
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• 2000

Field survey on the spatio-temporal distribution of water quality and chlorophyll a concentration, and the environmental factors on the variation of phytoplankton biomass were carried out at the 23 stations for four seasons in the Shiahae, southwestern coast of Korean Peninsula from February to October in 1995. I made an analysis on biological factor as chlorophyll a concentration as well as environmental factors such as water temperature, salinity and nutrients; ammonia, nitrite, nitrate, dissolved inorganic nitrogen, phosphate, N/P ratio, silicate and Si/P ratio. The waters in the Shiahae were not stratified due to the tidal mixing and high velocity of tidal current. And the high productivity in photic layer were supported by high nutrients concentration from freshwater on lands and bottom waters The low depth of transparency in the Shiahae had a bad influence upon primary production and marine biology. In Shiahae had a sufficient nutrients for primary production during a year. Especially dissolved inorganic nitrogen and silicate were high, the other side, phosphate was low. The source of nutrients in summer and silicate supply depend on input of freshwater from lands, the other side, dissolved inorganic nitrogen and phosphate were depend on rather supplied from bottom layer by the mixing and input of seawater from outside than input of freshwater from lands. Phosphate seemed to become a limiting nutrient for the primary production at all area of Shiahae in winter and at the northern parts in other seasons. However, dissolved inorganic nitrogen seemed to do it at the southern parts in other seasons except winter. Silicate didn't become a limiting nutrient for diatoms in Shiahae. Phytoplankton biomass as measured by chlorophyll a concentration was very high all the year round, it was controlled by the combination of the several environmental factors, especially of nitrogen, phosphorus and the physical factors such as light intensity. [Spatio-temporal distribution, Seasonal fluctuation, Nnutrients, Chlorophyll a, Environmental factors, Nutrient source, Limiting Nutrient, Light, Shiahae] .

• Journal of Food Hygiene and Safety
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• v.14 no.4
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• pp.372-379
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• 1999

A bacteriological study of sea water and oyster in Tongyeong coastal area was conducted to evaluate sanitary conditions of the bay and compliance of waters with the recommended bacteriological criteria fur the designated area of shellfish cultivation. The Samples were collected at 5 zone, 34 sampling stations(Fig. 1) established once a month from September 1997 to August 1998. During the study period, temperature ranged from 6.9 to 23.6$^{\circ}C$, transparency ranged from 2.6 to 6.2 m, chemical oxygen demand ranged from 1.35 to 1.82 mg/ι, dissolved oxy-gen ranged from 5.0 to 9.9 mg/ι, dissolved nitrogen ranged from 1.60 to 8.17 $\mu\textrm{g}$-at/ι, phosphate ranged from 0.14 to 1.21 $\mu\textrm{g}$-at/ι, Chlorophyll-a ranged from 2.03 to 69.9 mg/㎥, respectively. The coliform group and fecal coliform MPN's of sea water were ranged from <3.0~1,600 and <3.0~540, respectively. The coliform group and fecal coliform MPN's of oysters were ranged from <18~16,000 and <18~2,200, respectively. The viable cell counts in oyster ranged from $1.5\times$10$^2$to 8.2$\times$10$^3$. The coliform stoup, fecal coliform, classification of coliform group with IMViC reactions and pathogenic vibrios were analyzed. 437 strains that were obtained from Tongyeoung coastal area seawater samples represented E. coli group 47.5%, C. freundii group 14.8%, K. aerogenes 10.9%, unknown 26.8%, respectively. During the study period, infectious bacteria such as Vibrio ohoEerae, Salmonella sp. and Shigella sp. were not detected from the samples, but detection ratios of Vibrio parahaemolyticus and Vibrio vulnificus were 12~21% in summer months.

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.524-540
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• 2013

After the construction of the Geum River estuary dam, we investigated the ecological property of phytoplankton community with physicochemical property in 9 stations of Geum River estuary 4 times seasonally in 2010 about the environmental changes through the change of phytoplankton. Physicochemical property seems to show eutrophication which developed from the tendency of accruing of the nutrients (those involved in intermittency of freshwater which flows from the dike and the surrounding cities), caused by the changing of tidal flow, the decreasing of the inflow of seawater after the construction of the Geum River estuary dam (dike). A total of 233 species of species composition of phytoplankton emerged, showing a highly increased result compared to that of the former research. The reason for the increase in number is considered as a phenomenon resulting from the mix of blackish and freshwater marine species in the flow of intermittency of freshwater discharge. Also, the standing crops of phytoplankton considerably increased compared to when the dike had not yet been built. Highly affected by the discharge of occasional freshwater, the diatoms of blackish water zone dominated in spring and winter, while freshwater species appeared to dominate in summer and autumn. The species diversity showed over 2.0 except in winter season. After a cluster analysis, the result showed that the Geum River estuary was influenced by runoff from cities like Gunsan, the sewage which flows from Gyeongpo River, and the intermittency of freshwater discharge from the Geum River estuary dam.

• Food Engineering Progress
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• v.14 no.4
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• pp.299-306
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• 2010

This study was performed to develop new functional red ginseng drinks with Astragali Radix and Opuntia humifusa. Optimum extraction conditions such as solvent property and temperature for Astragali Radix were determined by distilled water vs. ethanol (95%) ratio (0:100, 25:75, 50:50, 75:25) and 60 vs. $80^{\circ}C$. Water-soluble extracts at $80^{\circ}C$ showed higher antioxidant activities than fat-soluble extracts at $60^{\circ}C$. Viscosities of 1-2% (w/v) of Opuntia humifusa solution were similar to that of the 0.1% guar gum solution. Addtion of Astragali Radix (3% and 5%, w/v) and Opuntia humifusa (1.2%, w/v), especially, had effect on the changes of pH of the red ginseng solution(5%, w/v) during storage for 7 days. A significant difference during the storage was shown in total plate counts by addition of Opuntia humifusa (1.2%, w/v) and microorganisms were reduced by six log cycles. Significant antiproliferation effects of red ginseng (5%, w/v) solution with Astragali Radix (3% & 5%, w/v) and Opuntia humifusa (1.2%, w/v) on Colon26m-3.1 carcinoma (colorectal carcinoma) cell and U87-MG neuronale glioblastoma (brain carcinoma) cell were not observed.

• Journal of Life Science
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• v.17 no.5 s.85
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• pp.728-734
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• 2007

In this study, we prepared surimi from pork leg and chicken breast by pH adjustments of 3.0 and 11.0. The content of crude protein, yield, water-holding capacity, redness, yellowness, myoglobin(Mb) and metmyoglobin(metMb) were significantly higher in the surimi manufactured from pork leg at adjustment pH 3.0 compared to the other surimi samples; whereas whiteness, myofibrillar protein, breaking force, deformation and gel strength were lower than other samples(P<0.05). The textural attributes were significantly higher in the surimi manufactured from pork leg at adjustment pH 11.0 compared to the other surimi samples; whereas Mb, metMb, cooking loss, breaking force, deformation and gel strength were lower than other samples(P<0.05). Again, the content of crude protein, yield, pH, breaking force, deformation, gel strength and lightness were significantly higher in the surimi manufactured from chicken breast at adjustment pH 3.0 compared to the other surimi samples; whereas myofibrillar protein, redness and metMb were higher than other samples(P<0.05). The content of myofibrillar protein, deformation, lightness and cohesiveness were significantly higher in the suriml manufactured from chicken breast at adjustment pH 11.0 compared to the other surimi samples; whereas Mb, cooking loss, yield and breaking force were higher than other samples(P<0.05). The chicken breast surimi had superior color and gel characteristics than manufactured from pork leg, and adjustment pH 11.0 had superior whiteness and cohesiveness than the pH 3.0 adjusted sample, however, there were no significant differences in sensory attributes among the surimi samples.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.3
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• pp.191-199
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• 2007

Seasonal variation in species composition of fish in the Saemangeum Reservoir was determined using seasonal samples collected by an otter trawl from April 2006 to February 2007 after the dike construction, and compared with the data obtained during the dike construction in 2001-2002. A total of 35 species, 8,960 individuals and 53,084.4 g of fish were collected during the study. Of the fish collected, brackish and coastal fishes such as Konosirus punctatus, Synechogobius hasta and Repomucenus lunatus, and migrant fishes such as Engraulis japonicus and Scomberomorus niphonius predominated in abundance accounting for 95% in the total number of individuals. Fish species composition and abundance showed a similar seasonal trend to those in the other western coastal waters of Korea. The resident species were mainly collected in spring and in autumn. The number of species and biomass were high in summer by the large amount of catch of migrating species. Catch was low in winter and only 2 species were collected. A brackish and coastal fish, K. punctatus and two migrant fishes, E. japonicus and S. niphonius were abundantly collected after the dike construction. However, the dominant fishes during the dike construction such as Leiognathus nuchalis, Neosalanx jordani and Chaeturichthys stigmatias were rarely collected. Fish density was $1,149\;inds./10,000m^2\;and\;12,644g/10,000m^2$ during the dike construction, and increased 6 times in the number of individuals ($7,467\;inds./10,000m^2$) and 3 times in biomass ($44,237g/10,000m^2$) after the dike construction. Annual species richness (R) and species diversity (H') decreased from R=0.0160 and H'=2.47 during the dike construction in 2001-2002 to R=0.0038 and H'=1.11 after dike construction in 2006-2006, respectively. These changes seemed to be related to the reduction of the saline area and degradation of water quality in Semangeum Reservoir after the dike construction.