• 한국환경과학회지
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• 제17권8호
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• pp.841-856
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• 2008

This research outlines a new method for evaluation of shellfish production in Gamak Bay based on the concept of EMERGY. Better understanding of those environmental factors influencing oyster production and the management of oyster stocks requires the ability to assess the real value of environmental sources such as solar energy, river, tide, wave, wind, and other physical mechanisms. In this research, EMERGY flows from environment sources were 76% for shellfish aquaculture in Gamak Bay. EMERGY yield ratio, Environmental Loading Ratio, and Sustainability Index were 4.26, 0.31 and 13.89, respectively. Using the Emergy evaluation data, the predicted maximum shellfish aquaculture production in Gamak Bay and the FDA (Food and Drug Administration, U.S.) designated area in Gamak Bay were 10,845 ton/y and 7,548 ton/yr, respectively. Since the predicted shellfish production was approximately 1.3 times more than produced shellfish production in 2005, the carrying capacity of Gamak Bay is estimated to be 1.3 times more than the present oyster production.

• Journal of Microbiology and Biotechnology
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• 제22권10호
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• pp.1412-1422
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• 2012

The aim of this work was to establish the optimal conditions for production of carboxymethylcellulase (CMCase) by a newly isolated marine bacterium using response surface methodology (RSM). A microorganism producing CMCase, isolated from seawater, was identified as Cellulophaga lytica based 16S rDNA sequencing and the neighborjoining method. The optimal conditions of rice bran, ammonium chloride, and initial pH of the medium for cell growth were 100.0 g/l, 5.00 g/l, and 7.0, respectively, whereas those for production of CMCase were 79.9 g/l, 8.52 g/l, and 6.1. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}7H_2O$, and $(NH_4)_2SO_4$ for cell growth were 6.25, 0.62, 0.28, and 0.42 g/l, respectively, whereas those for production of CMCase were 3.72, 0.54, 0.70, and 0.34 g/l. The optimal temperature for cell growth and the CMCase production by C. lytica LBH-14 were $35^{\circ}C$ and $25^{\circ}C$, respectively. The maximal production of CMCase under optimized condition for 3 days was 110.8 U/ml, which was 5.3 times higher than that before optimization. In this study, rice bran and ammonium chloride were developed as carbon and nitrogen sources for the production of CMCase by C. lytica LBH-14. The time for production of CMCase by a newly isolated marine bacterium with submerged fermentations reduced to 3 days, which resulted in enhanced productivity of CMCase and a decrease in its production cost.

• 한국수산과학회지
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• 제37권5호
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• pp.433-439
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• 2004

The movement range and diurnal behavior of red seabream (Chrysophrys major) tagged by the ultrasonic pinger externally were measured by acoustic telemetry techniques in the marine ranch of Tongyeong on 20 March to 30 October, 2003. The movement of fishes was monitored with the radio-acoustic linked positioning (RAP) buoy system and VR2 receivers. The test fishes showed behavior. pattern going to deep waters and returning to shallow waters. They moved gradually to deep waters as time goes by. They had a tendency to move wider range in night-time than day-time. It was found that the test fishes rarely showed a significant response to the artificial reefs.

• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1285-1289
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• 2016

A split-column photobioreactor (SC-PBR), consisting of two bubble columns with different sizes, was developed to enhance the photon utilization efficiency in an astaxanthin production process from Haematococcus lacustris. Among the two columns, only the smaller column of SC-PBR was illuminated. Astaxanthin productivities and photon efficiencies of the SC-PBRs were compared with a standard bubble-column PBR (BC-PBR). Astaxanthin productivity of SC-PBR was improved by 28%, and the photon utilization efficiencies were 28-366% higher than the original BC-PBR. The results clearly show that the effective light regime of SC-PBR could enhance the production of astaxanthin.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.364-367
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• 2005

This study investigated the production of antioxidant from Actinomyces culture supernatant. For the research of the natural marine antioxidant, several bacteria were isolated from the coast of Je-ju in Korea. An actinomycetes strains, S-1 was identified to a genus level 16S ribosomal DNA sequence and fatty acid analysis. From these results and other characteristics described in the Bergey's Manual, this strain was identificated as a Nocardiopsis dassonvillei. Strain S-1 showed high activity of 1,1-diphenyl-2-prcrylhydrazyl radical scavenging. The hydroxyl radical scavenging ability of Nocardiopsis sp. S-1 supernatant was 53%. Nutritional and cultural conditions for the production of antioxidant by this organism under shake-flask conditions have optimized. Similary initial medium pH 7.6, incubation temperature of $25^{\cicr}C$, sodium chloride concentration 2.5 and incubation time of 8 day were found to be optimal.

• Ocean and Polar Research
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• 제41권1호
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• pp.11-18
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• 2019

This acoustic experiment noted that fish species in Chilam-Gijang marine ranching area were more densely distributed in the pelagic zone during nighttime than daytime. In each season, the gill nets caught 15 different fish species and the estimated average target strengths were -44.0 dB and -44.4 dB for autumn and winter surveys, respectively. The estimated autumn fish biomass were 7.7 tons and 26.0 tons during daytime and nighttime, respectively. Winter biomass was 2.27 tons and 30.97 tons during daytime and nighttime, respectively. Different fish species form schools that exhibit different movements and behaviors, and thereby occupy varying water layers. These results explained the estimated fish biomass, and variation with seasons and time of the surveys around artificial reefs in Chilam Bay, Korea.

• 한국해양바이오학회지
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• 제6권1호
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• pp.31-40
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• 2014

The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design $2^6$ in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be $1040{\mu}E/(m^2{\cdot}s)$ light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.

• 한국미생물·생명공학회지
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• 제39권3호
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• pp.218-223
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• 2011

Eicosapentaenoic acid 생산 세균을 얻기 위해 1999~2000년 하계연구 기간 중에 남극 생물 및 침적토를 사용하여 600주의 균주를 분리하였고 TLC와 GC를 사용하여 오메가-3 고도불포화 지방산 EPA를 생산하는 미생물 7 주를 성공적으로 분리하였으며, 이중 EPA 생산이 가장 높은 L93 균주를 선발하였다. 16S rDNA의 염기서열 분석을 통하여 Shewanella 속으로 조사되었으며, 이에 분리된 균주를 Shewanella sp. L93라 명명하였다. EPA를 생산 최적 배양온도 $4^{\circ}C$이며, 초기 pH 7에서 최적 EPA 함량을 보였다. 아울러 염 농도는 50 %(w/v)에서 생산이 최대였다. EPA 최적 생산 조건을 이용하여 리터당 320 mg 생산할 수 있는 생산 시스템을 확립하였다. Urea 침전법과 HPLC을 이용하여 수율 72% 이상의 97% 순도를 가진 EPA를 정제할 수 있는 분리 정제 시스템 또한 본 연구를 통하여 확립하였다.

• 해양환경안전학회지
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• 제19권3호
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• pp.277-284
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• 2013

To ensure that the production system of a factory is efficient, the factory layout design should consider the location and material flow plans of facilities, workshops, and storage areas. Highly productive factories need to have an optimized layout planning process, and a customized design methodology of the production system is a necessity for feasible layout planning. This paper presents a method for designing a layout module's size and shape and provides a heuristic location-allocation algorithm for the modules. The method is implemented and validated using a rich internet application-based platform. The layout design method is based on the leisure ship production process; this method can be used for designing the layout of a new factory or remodeling an existing factory and its production system. In contrast to existing layout methods, the inputs required for the proposed method, such as target products, production processes, and human-resource plans, are simple. This layout design method provides a useful solution for the initial stage of factory design.

• 수산해양기술연구
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• 제46권1호
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• pp.32-49
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• 2010

Ecosystem-based fisheries management requires a holistic assessment of the status of fisheries by integrating fishery ecosystem indicators for management objectives. In this study four objectives were identified such as the maintenance of the sustainability, biodiversity and habitat quality and socio-economic benefits. The ecosystem-based fisheries assessment (EBFA) model to assess fisheries and their resources at the ecosystem level developed for Korean fisheries (Zhang et al., 2009) has a number of indicators for three management objectives. However, it was found that there were some overlapping components among indicators and that there were difficulties in assessing some indicators in the EBFA model. This study identified problems of the approach and suggested more pragmatic and simpler indicators. It also presented alternative reference points to assess indicators and discussed issues associated with the application of the EBFA model to a marine ranching ecosystem. In this study a total of 24 indicators were used for the assessment which included 4 socio-economic indicators. New indicators and reference points were demonstrated by applying it to the Uljin marine ranch.