• Title/Summary/Keyword: Marine Fish

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Studies on the Availability of Marine Bacteria and the Environmental Factors for the Mass Culture of the High Quality of Rotifer and Artemia 1. Change of Fatty Acid and Amino Aicd Composition During Cultivation of Rotifer, Brachionus plicatilis by Marine Bacteria Erythrobacter sp. $S\;\pi-I$ (고품질의 Rotifer와 Artemia의 생산을 위한 해양세균 이용과 대량생산에 따른 환경인자에 관한 연구 1. Erythrobacter sp. $S\;\pi-I$에 의한 Rotifer, Brachionus plicatilis의 배양시 지방산과 아미노산 조성의 변화)

  • LEE Won-Jae;PARK You-Soo;PARK Young-Tae;KIM Sung-Jae;KIM Kwang-Yang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.319-328
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    • 1997
  • To develop a beneficial microbial feed for the cultivation of rotifer, Brachionus plicatilis, an aerobic photosynthetic bacterium, Erythrobacter sp. $S\;\pi-I$ was isolated from marine structure at Haeundae beach in Pusan, Korea. Feeding effects of Erythrobacter sp. $S\;\pi-I$ on the growth of rotifer were analyzed comparing to other feeds such as PSB (purple nonsulfur bacteria), Chlorella sp. and baker's yeast. Erythrobacter sp. $S\;\pi-I$ contained more linoleic acid $(C_{18:3\omega3})$ and oleic acid $(C_{18:1\omega9})$ and amino acids than PSB (purple nonsulfur bacteria), Chlorella sp. and baker's yeast. The rotifer fed on Erythrobacter sp. $S\;\pi-I$ showed better effects than those fed on other feeds in the individual growth, size and weight. Also, the rotifer especially contained more eicosapentaenoic acid $(C_{20:5\omega3})$ and docosahexaenoic acid $(C_{22:6\omega3})$ in case of Erythrobacter sp. $S\;\pi-I$ feeding than the other feeds. In case of the feed of PSB and baker's yeast docosahexaenoic acid $(C_{22:6\omega3})$ did not show. In amino acid analysis, the rotifer fed on Erthrobacter sp, $S\;\pi-I$ showed more amino acid content comparing to those fed on other diets. Especially, arginine, isoleucine, histidine, lysine, methionine, phenylalanine, threonine, which are essential amino acid for fish growth, showed high contents. These results suggested that the aerobic photosynthetic bacterium, Erythrobacter sp. $S\;\pi-I$ would be a beneficial microbial teed for the cultivation of rotifer.

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Sex Differentiation of the Black Sea Bream, Acanthopagrus schlegeli (Bleeker) (감성돔, Acanthopagrus schlegeli (Bleeker)의 성분화)

  • Lee, Young-Don;Kang, Beob-Se;Lee, Jung-Jae
    • Korean Journal of Ichthyology
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    • v.6 no.2
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    • pp.237-243
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    • 1994
  • This work was conducted to study sex differentiation in the black sea bream, Acanthopagrus schlegeli (Bleeker), using a histological method for the appearance of primordial germ cell, formation of primitive gonads, differentiation of female and male from newly hatched larva to the ovotestis stage of fish. The 3~4 primordial germ cells of $6.8{\sim}7.2\;{\mu}m$ in size, which were buried under fibrous mesenchymal tissue between gut duct and notochord of pre-larva with a total length (T.L.) of 2.4 mm at 3 days after hatching. The proto-gonial cells were located in the epithelium of the coelom attached with pigment cells of juvenile with 6.4 mm in T.L. at 21 days after hatching. In juvenile of 20.8 mm in T.L. at 59 days after hatching, the proto-gonial cells were migrated to the retro-peritoneum through the lineshaped primitive gonad composed of fibrous mesenchymal tissue. In juvenile of 7.8 em in T.L. at 186 days after hatching, the mitotic division of proto-gonial cell appeared in the lineshaped primitive gonad having many eosinophilic granule cells and abundant fibrous connective tissue. In juvenile of 9.5 em in T.L. at 254 days after hatching, the gonad was occupied by abundant fibrous connective tissue, bundles of spermatocyte and spermatid. In juvenile of 10.5 cm in T.L. at 13 months after hatching, the gonad was divided into cortical layer and medullary layer. The former was composed of bundles of a few spermatocytes and proto-gonial cells, the latter was filled with the fibrous mesenchymal tissue and a few proto-gonial cells. In juvenile of 14.7 em in T.L. at 16 months after hatching, the gonad was separated into ovarian part and testicular part by the fibrous connective tissue. The ovarian part is consisted of ovarian cavity and oocytes of perinucleolus stage. The testicular part was occupied by spermatogonia in the cyst.

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Early Growth of Cultured Larval Haddock, Melanogrammus aeglefinus (Haddock, Melanogrammus aeglefinus 자어의 초기성장)

  • Park, In-Seok;Johnson, Stewart C.;Hur, Jun-Wook
    • Development and Reproduction
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    • v.10 no.4
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    • pp.271-275
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    • 2006
  • The objective of this study was to examine the early growth of haddock, Melanogrammus aeglefinus larvae from a series of reared specimens for provide information to developmental biology and more information on the aspect of aquaculture in the larvae of this species. Larvae were reared in the laboratory and sampled periodically for developmental study until 67 days after hatching. An increase in total length of fish indicated continuous growth, described by the growth expression of the type $TL=3.5374e^{0.0536X}(r^2=0.8759$, where TL is total length and X is at days after hatching) and $BW=0.0002e^{0.1858X}(r^2=0.8671$, where BW is body weight and X is at days after hatching), respectively. Pattern of body depth and pectoral fin length are instantaneous growth which expression of the type $BD=0.3545e^{0.0778X},\;r^2=0.9563$(where BD is body depth and X is at days after hatching) for body depth growth and the type $PL=0.0111e^{0.1591X},\;r^2=0.9194$(where PL is pectoral fin length and X is at days after hatching) for pectoral fin length growth. The relationship of body depth and total length expressed as $BD=0.2397X-0.5735(r^2=0.9957$, where BD is body depth and X is total length), and pectoral fin length and total length is $PL=0.1929X-1.3767(r^2=0.9882$, where PL is pectoral fin length and X is total length) pectoral fin length against body depth simultaneously recorded for juvenile haddock(PL=0.8117BD-0.9718, $r^2=0.9814$, where PL is pectoral fin length and BD is body depth). Relationship of body depth and body weight was expressed the type of $BD=-9.4734X^2+19.046X+1.3672,\;r^2=0.941$(where BD is body depth and X is body weight), and pectoral fin length and body weight expressed the type of $PL=6.379X^2+14.023X+0.3774,\;r^2=0.9494$(where PL is pectoral fin length and X is body weight). From this point view, growth characteristics of juvenile haddock in this experiment may be useful to establish a successful culture technique for rearing larval haddock.

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Environmental Tolerance for Pollutants in Littorina brevicula (Philippi) 1. The Acute Toxicity of TBTCl and Heavy Metals on Littorina brevicula (총알고둥 (Littorina brevicula(Philippi))의 오염원에 대한 환경내성 1. 총알고둥에 미치는 유기주석 및 중금속의 급성독성)

  • CHIN Pyung;LEE Jung Ah;SHIN Yun Kyung;LEE Jung Sick
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.5
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    • pp.587-592
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    • 1999
  • Littorina brevicula, a common small herbivorous gastropod, inhabits in almost every rocky and/or boulder shores of Korea with high density. The survival rates and oxygen consumption rates of this species exposed to tributyltinchloride (TBTCl), mercury (Hg) and cadmium (Cd) were investigated according to temperatures (15, $23^{\circ}C$) and individual sizes (12$\pm$0.5, 5$\pm$1 mm). At temperature $15^{\circ}C$, acute inhibitory concentrations for large (12$\pm$0.5 mm) and small (5 $\pm$1 mm) size individual showed that 13 day-$LC_{50}$ for TBTC1 were 0.87 and 0.65 ppm, respectively, 11 day-$LC_{50}$ of Hg were 5.55 and 2.85 ppm, respectively and 9 day-$LC_{50}$ of Cd were 13.77 and 8.46 ppm, respectively. At $23^{\circ}C$, acute inhibitory concentrations of pollutants on the large and the small size individual showed that 8 day-$LC_{50}$ of TBTCl were 0.68 and 0.15 ppm, respectively, 5 day-$LC_{50}$ of Hg were 10.41 and 5.73 ppm, respectively, and 4 day-$LC_{50}$ of Cd were 13.31 and 4.47 ppm, respectively. The order of toxicity on the species was TBTCl > Cd > Hg. Oxygen consumption rates during exposure to TBTCl, Hg and Cd toxicity were decreased more in small size than in large size individuals at 15 and $23^{\circ}C$.

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An Investigation of Microbial Contamination of Ready-to-Eat Products in Seoul, Korea (서울지역 유통판매 중인 즉석섭취.편의식품의 위해 미생물 오염도 조사)

  • Kim, Hee-Yun;Oh, Seon-Woo;Chung, So-Young;Choi, Seon-Hee;Lee, Ji-Won;Yang, Ji-Yeon;Seo, Eun-Chae;Kim, Yong-Hoon;Park, Hee-Ok;Yang, Cheul-Young;Ha, Sang-Chul;Shin, Il-Shik
    • Korean Journal of Food Science and Technology
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    • v.43 no.1
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    • pp.39-44
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    • 2011
  • This study was carried out to examine microbiological contamination of ready-to-eat products and to propose a draft-standard and specifications according to food types. RTE foods were classified into 6 groups including fish products, meat products, breads, rices, salads, and fresh cut foods. The prevalence rates of pathogens detected from all samples were compared among food categories. The pH ranges for all RTE samples were between 3.8 and 7.3. Total aerobic cell counts ranged from 2 to 6 log CFU/g. Bread, rice and fresh-cut foods showed significantly higher counts, which ranged above 4.0 log CFU/g among the samples. Two kinds of rice were above the level of the KFDA Food Code standard for Eschrichia coli. The prevalence rate of E. coli in the rice was 6.7%. For Staphylococcus aureus, one fish product and one bread-product had levels above 2 log CFU/g. Bacillus cereus counts for all samples were below the level of 3 log CFU/g. Listeria monocytogenes was not detected in the samples. Therefore, these data suggest that the primary microbial hazard factors for ready-to-eat foods and risk assessments should focus on E. coli, S. aureus, and B. cereus.

THE TASTE COMPOUNDS FERMENTED ACETES CHINENSIS (새우젓의 정미성분에 관한 연구)

  • CHUNG Seung-Yong;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.2
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    • pp.79-110
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    • 1976
  • In Korea fermented fish and shellfish have traditionally been favored and consumed as seasonings or further processed for fish sauce. Three major items in production quantity among more than thirty kinds which are presently available in the market are fermented anchovy, oyster and small shrimp. They are usually used as a seasoning mixture of Kimchi in order to provide a distinctive flavor. Fermented small shrimp, Acetes chinensis is most widely and largely used ana occupies an important position in food industry of this country. But no study on its taste compounds has been reported. This study was attempted to establish the basic data for evaluating taste compounds of fermented small shrimp. The changes of such compounds during fermentation as free amino acids, nucleotides and their related compounds, TMAO, TMA, and betaine were analysed. In addition, change in microflora during the fermentation under the halophilic circumstance was also investigated. The samples were prepared with three different salt contents of 20, 30 and $40\%$ to obtain the proper degree of fermentation at a controlled tempeature of $20{\pm}2^{\circ}C$. The results are summarized as follows: Volatile basic nitrogen increased rapidly until 108 days of fermentation and afterwards it tended to increase slowly. Amino nitrogen also increased rapidly until 43 days of fermentation and then increased slowly. Extract nitrogen increased and marked the maximum value at 72 day fermentation and then decreased slowly. ADP, AMP and IMP tended to degrade rapidly while hypoxanthine increased remarkably at 27 day fermentation but slightly decreased at 72 day fermentation. It is presumed that the characteristic flavor of fermented small shrimp might be attributed to the relatively higher content of hypoxanthine. In the free amino acid composition of fresh small shrimp abundant amino acids were proline, arginine, alanine, glycine, lysine, glutamic acid, leucine, valine and threonine in order. Such amino acids like serine, methionine, isoleucine, phenylalanine, aspartic acid, tyrosine and histidine were poor. In small shrimp extract, proline, arginine, alanine, glycine, lysine and glutamic acid were dominant holding $18.5\%,\;14.6\%,\;10.8\%,\;8.7\%,\;8.1\%\;and\;7.7\%$ of total free amino acids respectively. The total free amino acid nitrogen in fresh small shrimp was $63.9\%$ of its extract nitrogen. The change of free amino acid composition in the extract of small shrimp during fermentation was not observed. Lysine, alanine glutamic acid, proline, glycine and leucine were abundant in both fresh sample and fermented products. The increase of total free amino acids during 72 day fermentation reached approximately more than 2 times as compared with that of fresh sample and then decreased slowly. Fermented small shrimp with $40\%$ of salt was too salty to be commercial quality as the results of organoleptic test showed. It is found that 72 day fermentation with $20\%\;and\;30\%$ of salt gave the most favorable flavor. It is convinced that the characteristic flavor of fermented small shrimp was also attributed to such amino acids as lysine, proline, alanine, glycine and serine known as sweet compounds, as glutamic acid with meaty taste, and as leucine known as bitter taste. The amount of betaine increased during fermentation and reached the maximum at 72 day fermentation and then decreased slowly TMA increased while TMAO decreased during fermentation. The amount of TMAO nitrogen in fermented small shrimp was $200mg\%$ on moisture and salt free base. Betaine and TMAO known as sweet compounds were abundant in fermented small shrimp. It is supposed that these compounds could also play a role as important taste compounds of fermented small shrimp. At the initial stage of fermentation, Achromobacter, Pseudomonas, Micrococcus denitrificans which belong to marine bacteria were isolated. After 40 day fermentation, they disappeared rapidly while Halabacterium, Pediococcus, Sarcian, Micrococcus morrhuae and the yeasts such as Saccharomyces sp. and Torulopsis sp. dominated. It is concluded that the most important taste compounds of fermented small shrimp were amino acids such as lysine, proline, alanine, glycine, serine, glutamic acid, and leucine, betaine, TMAO and hypoxanthine.

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Microbiological Study and Isolation of the Vibrio vulnificus in the Sea Water, Sediment, Fish and Shellfish, Kithen Environment of Chunnam Coastal Area (전남 해안지역의 해수, 개펄, 수족관내 어패류 및 주방환경에서 장염 패혈증균(Vibrio vulnificus)의 분리와 미생물학적 연구)

  • Park, Kyung-Soo;Han, Chul;Suk, Keun-Young;Jung, Hae-Chang;Kim, Young-Hui;Kim, Chun-Kyu
    • Korean Journal of Food Science and Technology
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    • v.25 no.5
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    • pp.449-455
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    • 1993
  • Vibrio vulnificus is a recentry recognized halophilic organism that may cause human infections. Patients infected with Vibrio vulnificus often have a history of exposure to the sea, suggesting that the organism may be common inhabitant of marine environment. In this studies 314 samples were collected from 9 sites of Chonnam coastal area, from April to Jun, 1993. Isolation rates of V. vulnificus were 7.1% in total samples, 33.3% in sea water, 55.5% in sediment, 1.7% in fish and shellfish, 6.3% in kitchen environment. In each areas of Chunnam V. vunificus were isolated from 3.6% to 11.4%. The isolation rates of V. vulnificus was correlated positively with organic matter and COD in sea warer and sediment. In sea water, Microorganism population were $4.5{\times}10^3{\sim}3.5{\times}10^5\;CFU/ml$, Vibrio strains were $1.8{\times}10^l4.6{\times}10^3\;CFU/ml$, Coliform bacteria were $1.9{\times}10^1{\times}3.7{\times}10^2\;CFU/100ml$. In sediment, Microorganism population were $4.8{\times}10^3{\sim}5.2{\times}10^5\;CFU/ml$. Vibrio strains were $1.9{\times}10^2{\sim}8.4{\times}10^4\;CFU/ml$, Coliform bacteria were $7.2{\times}10^1{\sim}9.9{\times}10^2\;CFU/100ml$.

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Reproductive Cycle of a Rockfish, Sebastes schlegeli (조피볼락의 생식주기)

  • BAEK Jae-Min;HAN Chang-Hee;KIM Dae-Jung;PARK Chul-Won;Aida Katsumi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.5
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    • pp.431-438
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    • 2000
  • To clarify the annual reproductive cycle in a rockfish, Sebastes schlegeli, monthly changes in gonadosomatic index (GSI), hepatosomatic index (HSI) and histological feature of gonads and plasma levels of sex steroid hormones ($estradiol-l7{\beta},\;17{\alpha},\;20{\beta}-dihydroxy-4-pregnen-3-one,\;testosterone\;and\;11-ketotestosterone$) were investigated. The annual reproductive cycle in females could be divided into 5 periods as follows: 1) recovery period (June to September): serum level of $estradiol-l7{\beta}$ increased gradually; 2) vitellogenesis period (Septemer to february) : vitellogenic oocytes were obsewed, GSI sustained high value, and serum level of $estradiol-l7{\beta}$ increased; 3) gestation period (February-April): developing larva showed in the ovary, and serum levels of $17{\alpha},\;20{\beta}-dihydroxy-4-pregnen-3-one$ and testosterone increased; 4) partrition period (April to May) : larva were delivered, and value of GSI and serum levels of hormones decreased rapidly; 5) resting period (May to June) : value of GSI and serum levels of $estradiol-l7{\beta}$ and testosterone remained low. The annual reproductive cycle in males could be divided into 6 periods; 1) early maturation period (April to June): value of GSI and serum levels of hormones incresed gradually, cyst of spermatogonia incresed in number, and a small number of cyst of spermatocyte was observed; 2) mid-maturation perid (June to September); value of GSI and serum levels of hormones increased, and germ cells in many cysts were undergoing active sperrnatogenesis; 3) late maturation period (September to November) : value of GSI and serum levels of hormones remained high and spermatozoa were released into the lumina of the seminal lobules; 3) spermatozoa dischaging period (Nobember to December) : the lumina of the seminal lobules were enlarged and filled with mature spermatozoa; 4) degeneration period (December to Februauy)i value of GSI decresed and cyst of spermatocyte were decresed in number; 5) resting period (December to April) : no histological changes of testes were observed, and value of GSI and serum levels of hormones remained low. In November, the lumina of the seminal lobules were filled with mature spermatozoa and sperm masses were present in the ovarian cavity. Thus, copulation in this species occurred in November and December.

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Basic Studies on Developing Equipment for Waterless Transportation of Live Fish (활어의 무수 수송 장치 개발을 위한 기초적 연구)

  • CHO Young-Je;KIM Yuck-Yong;LEE Nam-Geoul;CHOI Yeung-Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.5
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    • pp.501-508
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    • 1994
  • This study was undertaken to get basic data for the cold-waterless transportation of live fish. The optimal cold temperature of plaice, Paralichthy olivaceus was determined by checking the changes of dissolved oxygen and ammonia in the sea water and the survival times during storage at various temperatures. After determination of optimal temperature for transportation, the changes of serum components and muscle components of live plaice were also carried out during storage at cold($5^{\circ}C$)-waterless conditions and the recovery conditions($10\%$ density at $15^{\circ}C$). At higher storage temperature, decreases in dissolved oxygen and the increases in ammonia in seawater were observed. In addition, the survival time was short at low temperature($0^{\circ}C\;and\;3^{\circ}C$). Almost all of the serum components(hemoglobin, glucose, LDH, GOT and GPT) of live plaice gradually increased during storage in cold-waterless conditions, and then those values decreased to the initial levels after $3{\sim}10hrs$ storage in conditions of recovery. The concentration of ATP in the muscle steadily decreased during storage in cold-waterless conditions. The contents of ADP and IMP seemed to be directly related to the extent of ATP breakdown. ADP and IMP thus showed a gradual increase during storage. The level of lactate in the muscles gradually increased during these storage times, also. On the other hand, the levels of those components in the muscle entirely recovered to their original levels within $3{\sim}6hrs$ storage after they were returned to conditions of recovery. The ratio of ATP to the ATP and its related compounds${ATP/(ATP+ADP+AMP+IMP){\times}100}$ in the muscle showed $45\%$ after 18hrs storage in cold-waterless conditions. Otherwise, ratios returned to their original levels within $3{\sim}6hrs$ of storage in recovery conditions.

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Egg Development and Morphology of Larva and Juvenile of Liparis tanakae in the Coastal Waters off Yeosu (여수 연안산 꼼치(Liparis tanakae)의 난발생 및 자치어 형태발달)

  • Kyung-Ae Jung;Na-Young Jeon;Sang-Hun Cha;Sung-Hoon Lee;Tae-Sik Yu;Keong-Ho Han
    • Korean Journal of Ichthyology
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    • v.35 no.4
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    • pp.263-269
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    • 2023
  • This study aims to contribute to the research on resource recovery for the rapidly declining population of Liparis tanakae by observing the larval development process and the morphology of juveniles based on their growth. Natural spawning eggs collected in Yeosu were used for observing the process of egg development and larval morphology. The water temperature during the rearing process was maintained at 12.3~13.5℃ (average 12.7℃). The fertilized eggs had an egg diameter ranging from 1.57 to 1.79 mm (average 1.71 mm) and were spherical and adhesive. Within 4 hours 35 minutes after fertilization, they reached the two-cell stage, and after 74 hours 10 minutes, the formation of the yolk sac began. At 106 hours post-fertilization, a caudal fin appeared at the tail tip. Hatching began at 526 hours, and the larvae developed with the yolk sac positioned just behind the eyes. The newly hatched larvae had both the mouth and anus open. Melanophores appeared inside the lower jaw and around the tail on the third day after hatching. By the 16th day after hatching, most of the yolk was absorbed, and melanophores were visible in the head region. Finally, on the 63rd day after hatching, the head region significantly developed, and the body shape and mouth were similar to those of an adult fish, signifying the transition to the juvenile stage. This study will serve as valuable data for aquaculture techniques related to the conservation and restoration of fish species based on the hatching and juvenile morphology of Liparis tanakae.