• 한국수산과학회지
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• 제46권5호
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• pp.552-558
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• 2013

This study was performed to determine effects of the supplementation of Kimchi lactic acid bacterial culture in extruded pellets (EP) on the growth, body composition, blood chemistry and immune response of olive flounder Paralichthys olivaceus. Four hundred eighty individuals averaging 16.1 g were randomly distributed into 12, 180 L flow-through tanks (forty fish per tank). Four concentrations of Kimchi lactic acid bacterial culture (KL) were prepared: Control (0%), 0.1%, 0.2% and 0.5%. Three concentrations (0.1%, 0.2% and 0.5%) of Kimchi lactic acid bacterial culture were each diluted to 10% of EP weight and then fully absorbed by EP for 10 minutes. Each diet was fed to triplicate groups of fish. Fish were hand-fed to apparent satiation twice a day for 8 weeks. At the end of the 8-week feeding trial, the plasma lysozyme and bacterial activities of fish were determined. In addition, the cumulative mortality of fish was monitored for 8 days after their artificial infection with Edwardsiella tarda. The weight gain, specific growth rate, feed efficiency ratio, protein efficiency ratio, protein retention, hepatosomatic index and condition factor of fish were not affected by dietary supplementation with KL. None of the proximate composition, plasma parameters, lysozyme or bactericidal activities of fish was affected by dietary supplementation with KL. However, the cumulative mortalities of fish fed EP containing 0.1% and 0.5% Kimchi lactic acid bacterial culture were relatively low compared to that of fish fed the control diet. In conclusion, dietary supplementation with KL did not effectively improve growth, feed utilization, body composition, plasma chemistry, lysozyme, bactericidal activities or immune response of olive flounder after E. tarda infection under these experimental conditions.

• 한국양식학회지
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• 제1권2호
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• pp.135-143
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• 1988

해산어류 및 갑각류의 유생사육시 먹이로 사용되는 rotifer(Brachionus Plicatilis)의 먹이생물로 이용되는 Chlorella중 어떤종이 대량사육에 가장 적합한가를 파악하기 위하여 해수산 Chlorella 2종, 담수산 Chlorella 4종에 대한 성장 및 영양염분석의 결과를 요약하면 다음과 같다. 1. 해수산 Chlorella중에서는 C. ellipsoidea가 f/2배지에서 가장 높은 성장을 나타냈고 담수산 Chlorella 중에서는 C. vulgaris가 Complesal에서 가장 높은 성장을 보였다. 2. 성장의 측면에서 볼 때 담수산인 C. vulgaris는 해수산인 C. ellipsoidea보다 specific growth rate가 1.68배나 높게 나타났다. 3. 영양염의 측면에서 볼 때 지방은 해수산인 C. ellipsoidea가 조단백과 회분은 담수산인 C. vulgaris가 더 높았다. 아미노산측정에서 $NH_3$의 함량이 담수산인 C. vulgaris가 해수산인 C. ellipsoidea보다 8배 이상 높은 점이 특이하였다.

• International Journal of Advanced Culture Technology
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• 제8권3호
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• pp.269-274
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• 2020

It has used buoy for efficient domestic farm operations and fishermen fish. Buoy uses IoT-based communication to transmit water temperature, salinity, humidity, wind speed, etc. to fishers in real time. In this paper, we utilize LoRa, which enables communication in the marine environment, to construct a network and apply it to an actual buoy for monitoring. The implemented LoRa uses the 900MHz band to configure the network. The sensor consisted of a sensor that can monitor the atmospheric environment and a sensor that can monitor the marine environment. In addition, the information received in real time will be provided to the fishing village host. The fishermen were fully aware of this and took appropriate measures to conduct sea trials.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2002년도 춘계 한국양식학회 학술대회 발표요지
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• pp.240.2-241
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• 2002

• 한국양식학회지
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• 제5권1호
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• pp.81-91
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• 1992

해산동물의 종묘생산시 초기단계의 먹이로써 미세조류의 배양은 매우 중요하다. 그러나 자연으로부터 먹이생물로써 필요한 미세조류를 순수분리하여 순종을 보관하는 일은 일반 양식 업자나 이 분야에 종사하지 않는 연구자에게는 매우 어려운 일이다. 따라서 미세조류를 필요로 하는 사람들에게 순종의 미세조류 strain을 공급할 수 있는 은행의 설립이 필요하다. 본 연구에서는 capilla교 pipette법, 한천도말법, 희석법을 이용하여 한국연안에서 80종의 미세조류를 분리하였다. 현재 부산수산대학교 양식학과 실험실에서 확보 배양중인 117종의 미세조류의 배양현황을 보고하였다.

• ALGAE
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• 제25권1호
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• pp.37-44
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• 2010

A tide-simulating apparatus was developed for culturing marine macroalgae. The objective of this study was to introduce a novel tide-simulating apparatus that can simulate a diurnal or semi-diurnal tidal cycle in the laboratory. In this apparatus, the seaweeds are move up and down and the water level remains the same during the simulated tidal cycle. The apparatus consists of 18 cylindrical culture tanks (3 blocks $\times$ 6 culture tanks) with 12 cm diameter and 24.5 cm long containing up to 2.5 L of seawater. There is a horizontal plate which covered all 18 culture tanks, and it is raised and lowered by a programmable motor that can regulate exposure time. In one application, seaweeds are attached to braided twine hung on Plexiglas air-tubing. The air-tubing is attached to a lid that is set on a horizontal plate. This apparatus is made of colorless Plexiglas to maximize light transmittance. This apparatus is easily disassembled and transportable to any indoor laboratory, wet laboratory, greenhouse, etc. This apparatus also offers considerable flexibility in terms of design. The size of culture tank can be redesigned by either increasing the height of cylinder or/and using a different diameter of cylindrical Plexiglas, therefore, larger/taller thalli can be cultivated. Growth rates of three eulittoral Porphyra species from different tidal elevations have been compared using this device.

• ALGAE
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• 제30권2호
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• pp.181-182
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• 2015

• 한국마린엔지니어링학회:학술대회논문집
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• 한국마린엔지니어링학회 2011년도 전기공동학술대회 논문집
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• pp.301-301
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• 2011

• 한국양식학회:학술대회논문집
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• 한국양식학회 2002년도 한일 일한 증양식 심포지엄 요약집
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• pp.35-36
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• 2002

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.16.1-16.8
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• 2016

To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at $28^{\circ}C$. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture.