• Archives of Pharmacal Research
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• 제29권11호
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• pp.942-945
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• 2006

A novel quinone derivative, Griseusin C (1), along with a known quinone, Naphthoquinone C (2), was isolated from the lyophilized culture broth of the marine-derived fungus Penicillium sp. The structures were elucidated on the basis of extensive 1D-and 2D-NMR, as well as HRESIMS, spectroscopic analysis. The relative stereochemistries of the compounds were assessed by NOESY analysis.

• 한국발생생물학회지:발생과생식
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• 제18권3호
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• pp.139-143
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• 2014

Different with other fishes, the guppies (Poecilia reticulata) is ovoviviparity, which retain their fertilized eggs within the follicle throughout gestation. The synchronously growing diplotene oocytes store nutrients in droplets and yolk, before their maturation and fertilization. The lecithotrophic strategy of development entails the provisioning of embryos with resources from the maternal yolk deposit rather than from a placenta, it allows the extracorporeal culture of guppy embryo. Studies on their early development of live bearers like the guppy including lineage tracing and genetic manipulations, have been limited. Therefore, to optimize conditions of embryo in vitro culture, explanted embryos from pregnant females were incubated in embryo medium (L-15 medium, supplemented with 5, 10, 15, 20% fetal bovine serum, respectively). We investigated whether the contents of FBS in vitro culture medium impact the development of embryos, and whether they would hatch in vitro. Our study found that in 5% of FBS of the medium, although embryos developed significantly slower in vitro than in the ovary, it was impossible to exactly quantify the developmental delay in culture, due to the obvious spread in developmental stage within each batch of eggs, and embryos can only be maintained until the early-eyed. And although in culture with 20% FBS the embryos can sustain rapid development of early stage, but cannot be cultured for the entire period of their embryonic development and ultimately died. In the medium with 10% and 15% FBS, the embryos seems well developed, even some can continue to grow after follicle ruptures until it can be fed. We also observed that embryonic in these two culture conditions were significantly different in development speed, in 15% it is faster than 10%. But 10% FBS appears to be more optimizing condition than 15% one on development process of embryos and survival rate to larvae stage.

• 한국해양바이오학회지
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• 제16권1호
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• pp.36-44
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• 2024

This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO₂. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO₂ fixation by increasing the availability of CO₂(HCO₃^-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m²/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO₂ availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO₂ fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1403-1406
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• 2007

An antiproliferative agent, streptochlorin, was isolated from the fermentation broth of a marine actinomycete isolated from marine sediment. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the strain belongs to the genus Streptomyces. Bioactivity guided fractionation of the culture extract by solvent partitioning, ODS open flash chromatography, and reversed-phase HPLC gave a pure compound, streptochlorin. Its structure was elucidated by extensive 2D NMR and mass spectral analyses. Streptochlorin exhibited significant antiproliferative activity against human cultured cell lines.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.663-667
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• 2001

A new secondary metabolite was isolated from the culture broth and mycelium of Streptomyces sp. collected from marine sediment. The structure of this compound was determined to be N-formylantimycic acid methyl ester, an acyclic derivative of antimycin, on the basis of combined chemical and spectral methods. The structure-activity relationship of antimycins is discussed.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권2호
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• pp.103-108
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• 2005

For more accurately describing the durations of the light and the dark phases of micro-algal cells over the whole light-dark cycle, and probing into the relationship between the liquid circulation time or velocity, the aeration rate and cell density, a series of experiments was carried out in 10 cm light-path flat plate photobioreactors. The results indicated that the liquid flow in the flat plate photobioreactor could be described by liquid dynamic equations, and a high biomass output, higher content and productivity of arachidonic acid, $70.10\;gm^{-2}d^{-1},\;9.62\%$ and 510.3 mg/L, respectively, were obtained under the optimal culture conditions.

• 수산해양교육연구
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• 제24권3호
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• pp.378-386
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• 2012

The objective of this study was to search the attributes and calculate the weights among the attributes considered to choose the best thing of various tourism resources focused on marine ranch of Chagwido in Jeju region. Through this analysis, this research provides the information for establishing development plan of Chagwido. To achieve this goal, this study analyzed the priority of experience tourism through the experts' survey with AHP methodology. The result showed that ecological experience (30.6%), fishing activities (23.1%), release of fish seed (14.9%) and culture experience (14.9%) among various tourism resources in marin ranch of Chagwido were in order of importance. Thus ecological experiences, activities related to fishing facilities and programs should be introduced in order to develop the marine ranch of Chagwido.

• 한국양식학회지
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• 제13권1호
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• pp.63-67
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• 2000

연안역에서 쉽게 관찰되는 해산 코페포다 Tigriopus Japonicus는 해산어의 종묘 생산 과정에서 동물 먹이 생물로서 이용되는 플랑크톤의 한 종이다. 그러나, 아직 T. japonicus의 대량 배양 및 안정 배양에 관한 기술은 미확립된 상태이며, 로티퍼와 함께 혼합 배양하는 방법이 비교적 다량의 T. japonicus의 수확이 가능한 것으로 알려져 있을 뿐이다. 이 연구에서는 로티퍼와이 혼합 배양조내에서 T. japonicus가 어떤 먹이원을 이용하여 성공적으로 증식 가능하였는지를 검토하였다. T. japonicus의 먹이로 급이한 Nannochloropsis oculata는 이용하지 않고 로티퍼 배양조의 부산물을 적극적으로 이용하지 않고. 로티퍼 배양조의 부산물을 적극적으로 이용하였다. 로티퍼 배양조의 부산물 먹이로 급이한 조건하에서 로티퍼는 그 증식이 크게 억제된 반면, 부산물만을 먹이로 급이한 T. japonicus의 어린 노플리우스 I 단계의 개체는 물론 코페포디드 I 단계의 개체도 정상적인 증식이 가능하였다. 난낭으로부터 갓 부화된 20개체의 어린노플리우스는 38일간의 로티퍼 배양조 부산물만의 단독 급이 조건하에서 배양 개시 후 30일째에 226.3${\pm}$24.1개체/40ml의 초고 밀도가 관찰된 것을 전후로 높은 밀도의 개체 유지가 가능하였다. 특히, 각 발생 단계별로 계수한 결과에서는 코페포디드 I단계에 로티퍼 배양조의 부산물만을 먹이로서 첨가한 결과에서도 유사한 결과가 관찰되었다. 결국 T. japonicus의 배양 밀도를 높이기 위해서는 노플리우스의 정상적인 성장을 도모하여 주어야 하며, 특히 노플리우스가 먹을 수 있는 상태의 먹이인 로티퍼 배양조의 부산물과 같은 먹이원이 코페포다의 배양 과정에 첨가되어야 할 것이다.

• 미생물학회지
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• 제41권1호
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• pp.74-80
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• 2005

제주도 연안의 해수로부터 항산화물질을 생산하는 세균을 분리하였으며 항산화물질의 특성을 조사하였다. 분리된 항산화 생성 균주는 그람양성의 통성혐기성균으로 운동성을 가진 단간균이었으며 생육시 NaCl를 필요로 하였다. 분리균주는 형태학적, 배양적, 생화학적 특성 및 165 rDNA 염기서열 분식결과 Exiguobacterium 속으로 동정되어 Ekiguobaoteriurn sp. SC2-1로 명명하였다. 항산화물질 생산균주 배양액의 radical 소거활성은 DPPH (1,1-diphenyl-2-picrylhydrazyl) 법을 사용하였으며, 항산화물질 생성 최적조건은 $25^{\circ}C,\;NaCl\;4\%,\;pH\;6\~8$ 이었으며, 최적 탄소원은 $1\%$ maltose였다. 배양액의 hydroxyl radical 소거활성은 $73\%$였으며 superoxide radical 소거활성은 $35\%$를 나타내었다.

• 한국식생활문화학회지
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• 제30권2호
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• pp.158-169
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• 2015

Tean Mado Shipwreck No. 3 is presumed to have been shipwrecked between 1260 and 1268. It departed from a Southern costal area of Yeosu in Jeonnam Province to Ganghwa Island, its final destination at which the temporal regime of Koryo Dynasty was located. In the shipwreck, a total of 35 wooden tablets were found, and forwarding places, senders, receivers, descriptions, and quantities of freight were written on the wooden tablets. The names of receivers included Kim Jun, who was influential in the late Musin Era of the Koryo Dynasty, and key institutions such as Junmin and Sambyulcho of the Musin force. Twenty wooden tables had lists of food items such as barley, abalone, salted-fermented abalone, mussel, dried mussel, salted fermented mussel, dried shark meat, fish oil, pheasant, and dried dog meat. The food items in the late 13th century were systematically examined using scientifically determined food organic remains and records of wooden tablets among the marine relics of Mado Shipwreck No. 3.