• 한국수산과학회지
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• 제45권4호
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• pp.367-371
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• 2012

To investigate the relationship between metabolic changes in $^1H$-nuclear magnetic resonance (NMR) spectra and fish vaccination, serum was collected from olive flounders treated with a formalin-killed Edwardsiella tarda vaccine and used for $^1H$-NMR metabolite profiling. Principal component analysis and partial least squares were applied to the $^1H$-NMR profile to reduce its complexity and establish class-related clusters. Relative lipid regions were distinguished in vaccinated and non-vaccinated serum. Then, the lipids were extracted from the serum and analyzed. Triolein was identified.

• 디지털산업정보학회논문지
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• 제19권3호
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• pp.165-175
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• 2023

Boryeong Mud Festival has become a representative local festival that both domestic and foreign tourists can enjoy together. In addition, it is one of the usual hands-on marine festivals in Korea that can be enjoyed with one mind at the Boryeong Mud Festival, regardless of race, age, and language. This study explored the overall perception and trends of the Boryeong Mud Festival using big data extracted online from the Boryeong Mud Festival. First, keywords such as Chungnam, hosting, summer, reporter, experience, opening ceremony, performance, operation, news, tourist, opening, event, and festival were frequently exposed online. Second, due to centrality analysis, the centrality of festival experience programs and performances, opening ceremonies, and Boryeong mayor was high. Third, due to the CONCOR analysis, five clusters of meaningful keywords related to the Boryeong Mud Festival were formed.

• 한국환경과학회지
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• 제17권9호
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• pp.949-956
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• 2008

The data of temperature, chlorophyll a, phytoplankton and NOAA/AVHRR satellite data were analyzed about 7 stations around Wando Island area on August 30, 2005. The sea water temperature range was from $15.19^{\circ}C$ to $24.97^{\circ}C$, and there was a cold water mass from the station 1 to 5 around the outside of Chungsando Island, the water temperature was lower at the bottom than surface. The salinity was $32.41{\sim}34.03$, DO was $7.40{\sim}9.14mg/L$, but the concentration of chlorophyll a was 1ug/L higher at the bottom than surface. Total phytoplankton appeared from the whole stations were 47 genus, 80 species and diatoms were dominant. A lot of dinoflagellates Ceratium forca and diatoms Thalassithrix spp. mostly appeared in the cold water mass were turned up from the station 1 to 5. 4 groups of phytoplankton clusters were shown in the surface, and 3 groups of phytoplankton clusters were shown at the bottom according to the water temperature. On the ground of the result analyzed with NOAA/AVHRR satellite data, the SST around Wando Island was $22{\sim}25^{\circ}C$, it was formed by thermohaline front latitudinally

• 한국해양바이오학회지
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• 제2권3호
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• pp.168-173
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• 2007

of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics. To analyze the transcriptome of olive flounder, Paralichthys olivaceus, we have conducted EST analysis using cDNA libraries made from muscle of P. olivaceus. Redundant ESTs were assembled into overlapping contigs by using the assembly program ICAtools software. We found that the 221 ESTs were composed of 21 clusters and 35 singletons, suggesting that the overall redundancy of the library was 74.7%. Of the 221 clones, 218 clones (98.6%) were identified as known genes by BLAST searches and 3 clones (1.4%) did not match to any previously described genes. Based on major functions of their encoded proteins, the identified clones were classified into 13 broad categories. Sequence analysis of the ESTs revealed the presence of microsatellite-containing genes which may be valuable for further gene mapping studies. This study contributes to the identification of many EST clones that could be useful for genetics and developmental biology of olive flounder.

• 한국패류학회지
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• 제25권1호
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• pp.5-13
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• 2009

Genomic DNA isolated from two geographical ark shell (Scapharca subcrenata) populations was amplified several times by PCR reactions. The ark shell population from Daecheon (ASPD) and from Wonsan (ASPW) in the West Sea and the East Sea of Korean Peninsula, respectively, obtained. The seven arbitrarily selected primers OPA-05, OPA-11, OPB-09, OPB-11, OPB-14, OPC-18 and OPD-07 were shown to generate the loci observed per primer, shared loci by each population, specific loci, unique shared loci to each population and shared loci by the two populations which could be clearly scored. Here, 862 loci were identified in the ASPD population, and 1,191 in the ASPW population: 137 specific loci (15.9%) in the Daecheon population and 84 (7.1%) in the Wonsan population. 407 shared loci by each population, with an average of 58.1 per primer, were observed in the ASPD population. 473 shared loci by each population, with an average of 67.6 per primer, were identified in the ASPW population. The numbers of specific loci in the ASPD and ASPW population were 137 and 84, respectively. Consequently, the average bandsharing value of individuals within the ASPW population was much higher than in the ASPD population. The bandsharing value between individuals' no. 08 and no. 13 was 0.628, which was the highest measured between the two geographical populations. The dendrogram obtained by the seven primers indicated three genetic clusters: cluster 1 (DAECHEON 01-DAECHEON 11), cluster 2 (WONSAN 12 and 14) and cluster 3 (WON SAN 13, 15, 16, 17, 18, 19, 20, 21 and 22). The genetic distance between the two geographical populations ranged from 0.043 to 0.499. Especially, individual no. 10 of Daecheon population was most distantly related to no. 14 of Wonsan population (genetic distance = 0.499).

• Journal of the Korean Data and Information Science Society
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• 제20권3호
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• pp.551-562
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• 2009

수협의 어선조업정보는 국가 공식 통계가 가지고 있지 못한 위치별 조업정보를 가지고 있다는 장점이 있다. 위치별 조업정보는 해당 지역의 어업피해보상, 자원가치 산출 등을 추정할 수 있어 국가통계자료로의 가치가 매우 높으나 어업인들의 자기 정보의 노출에 대한 기피로 인해 신뢰성이 떨어지는 단점을 지니고 있다. 본 연구는 유용성분석을 통해 이러한 수협의 어선조업정보의 활용을 위한 방안을 제시하고 위치별 조업패턴의 특성을 분류하여 해구별 유사성의 정보를 산출을 목적으로 하였다. 분석결과 수협의 어선조업정보는 정부 생산통계대비 어획량의 약 33% 수준이나 유용성 분석에서 그 패턴과 상관관계가 밀접해 위치별 패턴파악에 유용한 것으로 나타났다. 이를 바탕으로 대해구별 2단계 군집분석을 통해 어획량, 조업일수, 조업척수에 대해 각각 최적의 군집을 구분하고 이를 종합하여 8개의 군집으로 패턴을 구분하였다.

• 미생물학회지
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• 제53권2호
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• pp.137-140
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• 2017

Caballeronia sordidicola 균주 PAMC 26510은 남극 킹조지섬의 바톤반도에서 채집된 지의류인 Psoroma sp.에서 분리되었다. 균주 PAMC 26510의 초안 유전체 서열은 224개의 콘티그로 이루어졌으며, 총 7,872,143 염기쌍은 59.7% G+C 함량을 나타냈다. 유전체는 7,580개의 단백질 유전자, 6개의 rRNA 유전자와 46개의 tRNA 유전자를 포함하였다. 균주 PAMC 26510는 이전에 연구한 Caballeronia sordidicola에 속하는 북극 균주와 마찬가지로 대사능력에 있어서 광범위한 능력을 가지고 있는 것으로 여겨진다. PAMC 26510의 초안 유전체는 6개의 CRISPR arrays를 각각의 콘티그 상에 가지고 있으며, 이중 두 개의 콘티그 상에 CRISPR-관련 유전자가 연결되어 있었다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2015년도 춘계학술대회
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• pp.272-274
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• 2015

Success of communication at sea is directly linked with clear and complete delivery and receipt of the target message between interlocutors. It can be said that speakers' effective delivery of their intended message and listeners' precise decoding and accurate understanding are the keys to successful maritime communication. From this perspective, the scope of maritime English education and training needs to be reconceptualized and expanded into the area of communication itself, beyond the simple acquisition of, and familiarization with, IMO Standard Maritime Communication Phrases (SMCP). Therefore, in order to make learners' acquisition of marine communication knowledge more feasible, and the knowledge learned more practically applicable, training on effective and clear oral delivery should be also considered within the frame of maritime English education. Thus, critical training elements to realize this goal need to be suggested as guidelines. In this presentation, the theoretical background on this will be introduced in terms of English as a Lingua Franca (ELF) and Lingua Franca Core (LFC), which are the current mainstream forms of English communication in the international business context. Based on this, six key training elements will be discussed; that is, speech rate, word groups, pauses, nuclear stresses, consonants (including consonant clusters), and vowels (specifically long and short vowels). Finally, the practical pedagogical methods of each element, and its actual application into a real ESP classroom, will be suggested.

• 한국발생생물학회지:발생과생식
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• 제22권4호
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• pp.393-402
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• 2018

Genomic DNA was isolated from the Gracilaria vermiculophylla (GRV) and G. chorda (GRC) from Jangheung located in the southern sea of the Korean Peninsula, respectively and we performed clustering analyses, DNA polymorphisms and the genetic differences. The seven selected primers OPC-01, OPA-04, OPA-05, OPD-07, OPD-08, OPB-10, and OPD-16 generated average bandsharing (BS) value, the genetic distance and dendrogram. The size of DNA bands varies from 90 bp to 2,400 bp. The average BS value was $0.859{\pm}0.004$ within GRV and $0.916{\pm}0.006$ within GRC. The average BS value between two Gracilaria species was $0.340{\pm}0.003$, ranged from 0.250 to 0.415. The dendrogram obtained by the seven primers, indicates two genetic clusters. The genetic distance between two Gracilaria species ranged from 0.059 to 0.513. The individual VERMICULOPHYLLA no. 07 of GRV was genetically closely related to VERMICULOPHYLLA no. 06 of GRV (genetic distance=0.059). Especially, two entities between the individual VERMICULOPHYLLA no. 10 of GRV and CHORDA no. 22 of GRC showed the longest genetic distance (0.513) in comparison with other individuals used. Accordingly, as mentioned above, PCR analysis showed that the GRV was a little more genetically diverse than the GRC species. We convinced that this DNA analysis revealed a significant genetic distance between two Gracilaria species pairs (p<0.01).

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제2권3호
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• pp.166-169
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• 2021

The purpose of this study was to identify the natural habitat of Bulbophyllum inconspicuum Maxim. in Hongdo, Jeonnam Province, South Korea. A field investi gation was conducted between May 2019 and May 2021. The growth of B. inconspicuum species was confirmed in three known regions based on National Survey on the Distribution of Endangered Species. Additionally, in the area investigated, 37 new regions harboring B. inconspicuum were detected. The newly observed B. inconspicuum grew naturally on Quercus mongolica, Rhaphiolepis indica var. umbellata, Carpinus turczaninowii, and rocks. Most of them were found on southeast and southwest slopes with altitude above 250 m. As few as 10 up to hundreds of B. inconspicuum were found in clusters. Close to their habitat, Calanthe sieboldii Decne. ex Regel, C. aristulifera Rchb. f. and C. × kibanakirishima Maek. were also found. As Hongdo is an island, multiple endangered species were found together. Thus, once the habitat of a certain plant is discovered, it is very likely that illegal collection of several other species will also occur. Proactive internal and external measures are needed to preserve these species.