• Molecules and Cells
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• 제45권5호
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• pp.284-290
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• 2022

Exosome, a type of nanoparticles also known as small extracellular vesicles are gaining attention as novel therapeutics for various diseases because of their ability to deliver genetic or bioactive molecules to recipient cells. Although many pharmaceutical companies are gradually developing exosome therapeutics, numerous hurdles remain regarding manufacture of clinical-grade exosomes for therapeutic use. In this mini-review, we will discuss the manufacturing challenges of therapeutic exosomes, including cell line development, upstream cell culture, and downstream purification process. In addition, developing proper formulations for exosome storage and, establishing good manufacturing practice facility for producing therapeutic exosomes remains as challenges for developing clinical-grade exosomes. However, owing to the lack of consensus regarding the guidelines for manufacturing therapeutic exosomes, close communication between regulators and companies is required for the successful development of exosome therapeutics. This review shares the challenges and perspectives regarding the manufacture and quality control of clinical grade exosomes.

• 한국조리학회지
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• 제23권6호
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• pp.20-26
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• 2017

To guarantee the safety of rice cake production, the Hazard Analysis Critical Control Points system was applied to the production process. The purpose of this study was to analyze the microbiological hazards of the manufacture of rice cakes, and establish critical limits in the process of the manufacturing rice cakes. To control the microbiological hazards, the sterilization process was set to a critical limit. The process of manufacturing rice cakes can reduce these microbiological hazards during the steaming process. A microorganism test for each specimen was conducted three times and compared with before and after steaming processes. The finished product was conducted by microbiology experiment and the validity of the steaming process was verified. The results were determined to be capable of reducing the biological element of Critical Control Point via the steaming process. Microbiology such as aerobic plate count, coliform, Escherichia coli(E. coli), Listeria monocytogenes, Enterohemorrhagic E. coli, Salmonella spp., Bacillus cereus, Staphylococcus aureus and Clostridium perfringens was evaluated by the experimental method of Korean Food Standards Codex. Aerobic plate count was reduced by steaming process, and no microorganism were detected. All rice cakes in the finished product were judged to be safe for both the Escherichia coli and general bacteria. In conclusion, it suggested that a HACCP plan was necessary for management standard and systematic approach in the establishment of critical limit, problem resolution, verification method, education, and records management. Based on this study, it is intended to provide a baseline for improving quality control standards and improving hygiene levels for small manufacturers.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2002년도 춘계학술대회 논문집
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• pp.536-542
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• 2002

There are two important variables in machining process control, which are feed and cutting speed. In this work, a two degree-of-freedom controller is designed and implemented to achieve on-line cutting force control and position control based on the modelling of cutting process dynamics which are established through step response test. Two schemes are proposed and implemented. The first is feed control under the constant spindle speed and spindle speed control under the constant feed speed. The second is a simultaneous control of feed and spindle speed. The last performs a position control under the constant cutting force. Those are confirmed to work properly. Especially the latter shows a faster response.

• 한국기계가공학회지
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• 제7권2호
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• pp.23-30
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• 2008

As a basic machining process, turning is a widely used machining process in which a single-point cutting tool removes material from the surface of a rotating material. A common method of evaluating machining performance is to measure the surface roughness. In a turning operation, it is important to select cutting conditions for achieving high cutting performance. As a rule, cutting conditions can be classified into feed rate, depth of cut and insert radius. While cutting process even though cutting conditions are optimized, the average roughness can be deterioration due to wear of the cutting tool edge. In this study, the aim is to maintain the average roughness even though the cutting condition is irregularly changing within the predictable range due to the working environment. First, the surface roughness model influenced by cutting conditions is constructed based on the experimental results in a turning operation, Second, applying the sliding mode control theory to the turning operation model which is composed of the surface roughness model and the motor transfer function, the surface roughness is closed to the desired value. Finally, the effectiveness of this approach is demonstrated through the computer simulation.

• 한국공작기계학회논문집
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• 제17권6호
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• pp.49-55
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• 2008

Arc welding process is one of the most important technologies to join metal plates. Robotic welding offers the reduced manufacturing cost sought, but its widespread use demands a means of sensing and correcting for inaccuracies in the part, the fixturing and the robot. A number of problems that need to be addressed in robotic arc welding processes include sensing, joint tracking, and lack of adequate models for process parameter prediction and quality control. Problems with parameter settings and quality control occur frequently in the GMA(Gas Metal Arc) welding process due to the large number of interactive process parameters that must be set and accurately controlled. The objectives of this paper are to realize the mapping characteristics of bead width using a sensitivity analysis and develop the neural network and multiple regression method, and finally select the most accurate model in order to control the weld quality(bead width) for fillet welding. The experimental results show that the proposed neural network estimator can predict bead width with reasonable accuracy, and guarantee the uniform weld quality.

• 품질경영학회지
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• 제41권3호
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• pp.487-494
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• 2013

Purpose: In this study, we propose the precision guided munitions verification methodology using the statistical analysis method has been proposed. and it can be applied to the precision guided munitions quality assurance work. Methods: This modeling is based on Failure Mode and Effects Analysis, Statistical Process Control, Defense Quality Managerment System, Production Readiness Review, Manufacturing Readiness Assesment and so on. Results: The Process Control Modeling that has the following procedures ; searching the critical to quality, statistical analysis by process, verify process. Moreover, the effectiveness of the methodology is verified by applying to the precision guided munitions. Conclusion: To achieve a analysis methods of statistical process control and verify process for precision guided munitions.

• 한국기계가공학회지
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• 제16권1호
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• pp.112-117
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• 2017

The arc, generated by Tungsten Inert Gas(TIG) welding, is stable and provides excellent quality of the weld. Since automation is difficult, a lot of work is performed by hand. In addition, to obtain the uniform weld quality is difficult when using a base metal having a nonuniform welding line, or when welding inside a pipe. Generally, TIG welding power has the characteristic of constant-current. The welding voltage is changed in proportion to the arc length. Hence, the automatic voltage control equipment should be applied at the TIG welding system. The automatic voltage control equipment has been designed using LabVIEW software. It consists of a manufactured voltage divider circuit, and jig for moving the torch. The voltage measurements and driving of the motor were performed through the algorithm implementation in LabVIEW. Welding was conducted while increasing the arc length. In this process, it was confirmed that the automatic voltage control equipment kept the arc length constant.

• 한국기계가공학회지
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• 제10권4호
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• pp.121-126
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• 2011

An accumulator placed on the refrigerant cycle pipe lines is a part to relax fluctuations of pressure within the pipe lines and stabilize refrigerants flowed into pipe. The accumulator has been mainly manufactured by the process of tube spinning using CNC(Computer Numerical Control) lathe. However, this process has the defects which are low productivity per hour and high cost. For that reason, tube end-forming using roll die is actively being developed, recently. The purpose of this study is to develope the tube end-forming process using roll die in order to manufacture the accumulator for the refrigeration pipe lines. First, the process design of tube end-forming was performed based on specification of product, and then was verified with FE analysis. Also, the effects of friction coefficient and revolution speed of roll die on forming load were investigated. The analytical results were applied in the final process design of tube end-forming. Finally, tube end-forming test was carried out to verify the validity of the FE analysis and the process design.

• 산업경영시스템학회지
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• 제17권32호
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• pp.137-144
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• 1994

According to the cementing process, adhesive for dress shoe applicable to the style of ladies' has steadily been developed. In the study on the evaluation, mathematical model is presented. In the study on the quality control, a list of test results from field application and a model of manufacturing process including adhesion process are introduced.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 1999년도 추계학술대회 논문집 - 한국공작기계학회
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• pp.27-33
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• 1999

In order to regulate cutting force at a desired level during NC lathe process, a feedrate override Adaptive Control Constraint system was developed. Nonlinear model of the cutting process was linearized as an adaptive model with a time varing process parameter. Performance of the ACC system was confirmed on the NC lathe equipped with the developed NC system through a large amount of experiment.