• 한국미생물·생명공학회지
• /
• 제49권1호
• /
• pp.111-119
• /
• 2021

본 연구에서는 국내 자생 식물인 아욱으로부터 새로운 균주를 분리 및 동정하였고 해당 미생물이 생산하는 항미생물질의 활성과 관련 생합성 유전자들을 확인하고자 하였다. 16S rRNA 유전자 서열 정보를 토대로 비교한 결과, 아욱에서 분리된 균주는 Bacillus velezensis이었으며 strain은 MV2라고 명명되었다. 유전체 염기서열 분석을 통해 전체 유전정보를 확인할 수 있었으며, 45.57% GC 함량을 가지는 4,191,702 bp 크기의 1개 컨티그(contig)가 존재하는 것으로 확인되었다. B. velezensis MV2가 정지기에 생산하는 물질 중 항균 활성이 확인된 소수성 물질 분획을 이용하여 항균 활성 스펙트럼 테스트를 진행한 결과, 그람음성균보다 그람양성균에서 더 높은 억제능이 확인되었다. 6종의 곰팡이를 이용한 항진균 활성 테스트에서는 모든 진균에 대해 강한 저해 활성을 보였으며, 특히 F. fujikuroi와 F. graminearum에 대한 항진균 활성이 매우 강하게 나타났다. 세균에 대한 항균물질의 작용 기작 분석을 통해 해당 항균물질은 균을 용해시키는 살균(bactericidal) 특성을 가진 것으로 추측할 수 있었다. B. velezensis MV2의 유전체 염기서열 정보를 통해 이차대사산물 생합성 유전자 cluster를 탐색한 결과 총 47가지 이차대사산물 생산이 예측되었으며, 기존에 밝혀져 있는 물질들과 유사도 80% 이상인 물질은 14개로 확인되었다. 앞서 확인된 내용들을 바탕으로 B. velezensis MV2에서 생성되는 항균물질은 비리보솜 펩타이드성 물질로 예상되며, 향후 항균물질의 동정과 활용 가능성에 대한 추가적인 연구가 필요할 것으로 생각되었다. 기존에 상업적으로 이용되었던 곰팡이에 대한 활성이 낮은 항균물질 생물제제들과 함께 복합기능성 미생물제로 활용하여 식품산업 및 농업에서의 이용 가능성이 있음을 보여주었다.

• 청정기술
• /
• 제21권1호
• /
• pp.39-44
• /
• 2015

본 연구에서는 다섯 종의 씨앗(춘채, 아욱, 양배추, 배추, 당근)을 이용하여 금속산화물 나노입자(CuO, NiO, Fe₂O₃, Co₃O₄, TiO₂, ZnO)들이 씨앗발아와 발아지수에 미치는 영향을 조사하였다. CuO, ZnO, NiO 나노입자는 씨앗 활성에 뚜렷한 독성 영향을 나타내었으며, 나노입자종류에 따라 상이한 민감도를 나타내었다. 각 나노입자에 대한 독성순서(EC₅₀ 범위)는 다음과 같다: CuO 6~27 mg/L > ZnO 16~86 mg/L > NiO 48~112 mg/L. 나머지 조사 대상 나노입자인 Co₃O₄, TiO₂, Fe₂O₃은 최대 1,000 mg/L 높은 농도 노출에도 뚜렷한 영향을 나타내지 않았다. 씨앗별 상이한 민감도를 나타내었으며, 가장 민감한 종인 아욱의 씨앗발아 EC₅₀은 CuO 5.5 mg/L ZnO 16.4 mg/L, NiO 53.4 mg/L로 조사되었다. 씨앗별 나노입자에 대한 독성 영향은 CuO > ZnO > NiO > Fe₂O₃ ≈ Co₃O₄ ≈ TiO₂ 나타났으나, 당근씨앗은 NiO [EC₅₀ 80.4(71.41~90.54) mg/L]와 ZnO [EC₅₀ 85.8(69.31~106.29) mg/L]가 유사한 독성을 나타내었다.

• 한국식품영양학회지
• /
• 제3권1호
• /
• pp.69-79
• /
• 1990

Koryo(918-1352) made a certain progress in her culture and agriculture except the latter part of the era when the development in social economy were retarded due to military dictatorship and Mongol invasion. Despite of these external conditions, cultivations and kinds of horticulture foods were expanded With the help Of many king's agriculture first policy and the advancement in cultivating method . Among the horticulture foods, fruits such as peach, plum, Japanese apricot, apricot, cherry, pear, persimmon, pomegranate, crab apple, Jujube, grape, Chinese quince, walnut, orange, yuzu, chestnut, ginkgo nut(silvernut), pine nut, nutmeg nut, and fiat were crowed, and vegetables such as Chinese cabbage, turnip, radish, garlic, welsh onion, gynmigit, scallion, taro, malva, cucumber, white gourd, bottle gourd, water melon, eggplant, Japanese ginger, ginger and litchi were cultivated, while semi, water shieled and bamboo sprouts were taken in natural. Fruits were taken in natural or through dry, and particularly grapes were used to make wine, Flowers of Japanese apricots, some fruit trees, and chrysanthemum were also made into wine. Certain fruits were used as medicine owing to its medicinal nature. Vegetables were used to make Kimchi or to boil soup, sometimes they were dried to be kept in storage to be used in rare season and also used as medicine Increase in kinds of horticulture foods does not have any direct relation with the reform of social economy, but the fact that so many kinds of horticulture foods were cultivated and used in Koryo era shows that they elevated people's life and dietary culture.

• Journal of Forest and Environmental Science
• /
• 제30권3호
• /
• pp.253-258
• /
• 2014

The study attempts to assess the status, distribution, conservation and use value of Medicinal and Aromatic Plant (MAPs) in the Sagarmatha National Park. Altogether 62 species of MAPs belonging to 47 genera and 33 families have been recorded in the study area. 10 species, belonging to 9 families are categorized as the potential species. Out of the these species, most potential in local but threatened species are Allium hypsistum Stearn, Cordyceps sinensis Sacc, Dactylorhiza hatagirea Soo, Nardostachys grandiflora DC, Aconitum orochryseum, Ephedra gerardiana Wall. Ex. Stapf, Swertia multicaulis D. Don, Picrorhiza scrophulariflora Penne, Rheum australe. D. Don, Malva verticillataL and Swertia pedicallata Benerji. By analysis of data using Simpson's diversity index (SI) and Shannon weaver function (H'), there was high diversity (more heterogeneous) MAPs species composition in the Manjo Gate to Large Dobhan. (0.98349). Less diversity (less heterogeneous) MAPs species composition was in Tyanboche to Pangoche (0.90419). Similarly, the Shannon weaver function shows that in plots laying out in Mongla to Phorche are evenly distributed than others However, higher MAPs species (i.e., 31) was found in the way of Manjo Gate to Larja Dobhan than others.

• 한국환경복원기술학회지
• /
• 제20권5호
• /
• pp.15-41
• /
• 2017

The purpose of this study is to present the basic data for conservation of forest ecosystem by surveying and analysing the flora distributed in Nakdong-Jeongmaek Mountains, Korea. The numbers of flora were 901 taxa including 116 families, 433 genera, 790 species, 4 subspecies, 90 varieties, 15 forms and 2 hybrids. The threatened species was Pedicularis ishidoyana. The rare plants were 33 taxa including Pseudostellaria sylvatica, Cimicifuga heracleifolia var. bifida, Isopyrum mandshuricum, Paeonia japonica, Syringa patula var. kamibayshii, Lilium distichum, Iris odaesanensis and so forth. The Korean endemic plants were 35 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Corydalis maculata, Lespedeza maritima, Cirsium setidens, Carex okamotoi and so forth. The specific plants by floristic region were 155 taxa including 55 taxa of grade I, 41 taxa of grade II, 36 taxa of grade III, 18 taxa of grade IV and 5 taxa of grade V. The naturalized plants were 62 taxa including Rumex obtusifolius, Amaranthus lividus, Medicago sativa, Geranium carolinianum, Malva sylvestris var. mauritiana, Foeniculum vulgare, Sonchus oleraceus, Tradescantia reflexa and so forth. The invasive alien plants were 5 taxa including Rumex acetocella, Solanum carolinense, Ambrosia artemisiifolia, Aster pilosus and Lactuca scariola.

• Plant Resources
• /
• 제6권2호
• /
• pp.114-119
• /
• 2003

In this study, an on-site survey was conducted around Gasado island at Jodo-myeon, Jindo-gun, Jeonnam during July 2 to 25, 2002. The naturalized plants investigated in the surveyed sites were revealed 15 families and 39 species, including Althaea rosea and Oxalis articulata, which are ornamental plants. Only 10 species appeared in Imjado island among the surveyed sites the surveyed sites and Imjado Island, including Elymus sibiricus, Lolium perenne, Chenopodium album, Oxalis articulata, Oxalis corymbosa, Oenothera odorata, Xanthium strumarium, Bidens frondosa, Ambrosia artemisiaefolia var. elatior, and Taraxacum officinale. 6 species were distributed only in Gasado island, Jindo-gun, which included include Festuca myuros, Chenopodium ambrosioides, Amorpha fruiticosa, Malva neglecta, Calendula arvensis, and Carthamus tinctorius. 18 species were showed in Soheuksan island.; Yeosu area, 29 species; Imja Island in Sinan-gun, 34 species, and Gasado Island in Jindo-gun revealed 39 species. It is believed that the species of naturalized plants have been expanded compared to the other areas because of close location to the inland and large population fastiy. The Urbanization Index(UI) of this area was found to be 19.5. The naturalized plant communites in these surveyed sites were classified into Rumex crispus, Elymus sibiricus, and Lolium perenne community according to the vegetation table. Furthermore, they formed a community around areas that received great artificial influence from human dwellings or roads due to the characteristics of species distribution

• 한국유기농업학회지
• /
• 제19권spc호
• /
• pp.230-233
• /
• 2011

We collected and examined germplasm resources of lettuces, edible chrysanthemums and mallows for organic farmers. We found that 3 genotypes (OL-51, OL-52, and OL-63) are suitable for organic cultivation. The main use of treatment for grey mold among eco-friendly pest control methods is helpful to increase efficiency of seed-gathering after collecting and evaluating a total of 64 kinds of lettuces-collected 43 South Korea local varieties, 8 kinds cultivated by the Highland Agriculture Research Center, 4 kinds of cultivated lettuces, and 9 kinds for sales- to choose appropriate kinds of lettuces for organic cultivation in South Korea. We chose those with more than 300 leaves (OC-18, OC-44, and OC-51) after evaluation of yield characteristics of a total of 62 edible chrysanthemums. We covered each genotype of seed of edible chrysanthemums when gathering the seeds by ourselves so as not to decrease the purity of each genotype. We chosed OM-38, OM-39, and OM-40 for their yield performance and germination rates among the total 41 genotypes of mallows. We selected 9 genotypes of lettuces, editable chrysanthemums and mallows with possibility of organic cultivation and found that they can be used permanently through self seed-gathering on organic farms.

• 한국식품조리과학회지
• /
• 제21권2호
• /
• pp.190-194
• /
• 2005

This study was conducted to investigate the changes in contents of total flavonoid and total polyphenol of three leafy vegetables, spinach(Spinacia oleracea L.), chard(Beta vulgaris L.), whorled mallow(Malva verticillata L.) by various blanching times. Total flavonoid and total polyphenol contents of spinach were 21.01 and 25.58 mg/g, those of chard were 26.02 and 35.14 mg/g, and those of whorled mallow were 16.80 and 19.94 mg/g, respectively. Total flavonoid and total polyphenol contents of chard were the highest among the leafy vegetables. Total flavonoid and total polyphenol contents of leafy vegetables were decreased according to blanching time. Especially, total flavonoid content of chard showed significant loss of $15-30\%$ by blanching time. Total polyphenol content of whorled mallow showed loss of $37-39\%$, but the decrease was not significant.

• 생약학회지
• /
• 제39권3호
• /
• pp.213-217
• /
• 2008

Oxidative stress is considered to play an important role in a variety of neurodegenerative disorders of central nervous system. The immortalized mouse hippocampal cell line, HT22, phenotypically resembles neuronal precursor cells but lacks functional ionotropic glutamate receptors, thus excluding excitotoxicity as a cause for glutamate triggered cell death. Therefore, HT22 cells are a useful model for studying oxidative glutamate toxicity. In this study, we examined whether the methanol extracts of some native plants at Mt. Baekdu could protect HT22-immortalized hippocampal cells against glutamate-induced oxidative stress. Seventy-eight plants sources were collected at Mt. Baekdu, and extracted with methanol. These extracts had been screened the protective effects against glutamate-induced oxidative damage in HT22 cells at the 100 and 300 ${\mu}g/ml$. Of these, thirteen methanolic extracts, Acer mono (leaf), Artemisia stolonifera (aerial part), Carduus crispus (aerial part), Carex mongolica (whole plant), Clematis hexapetala (whole plant), Galeopsis bifida (aerial part), Galium verum (whole plant), Ganoderma lucidum (whole plant), Ixeris chinensis (whole plant), Malva verticillata (aerial part), Polygonum senticosum (whole plant), Rebes mandshricum (branch), and Taraxacum mongolicum (aerial part), showed significant protective effects against glutamate-induced oxidative damage in HT22 cells.

• 한국응용곤충학회지
• /
• 제45권3호
• /
• pp.339-346
• /
• 2006

비름과 쇠무릎등 총 36과 81속 90종의 약용식물을 대상으로 당근뿌리혹선충의 감염 여부를 조사한 결과, 쇠무릎 등 70종의 식물에서 당근뿌리혹선충 감염을 확인하지 못했고, 이들 70종 중 42종의 식물을 당근뿌리혹선충에 의한 더덕의 피해가 많았던 포장에 심고, 감염여부를 조사한 결과, 쇠무릎 등 26종은 당근뿌리혹선충에 거의 감염되지 않았고, 지치 등 16종은 감염되었음을 확인하였다. 또한 42종의 식물중 30종의 식물을 Pot 재배를 통하여 당근뿌리혹선충 접종밀도별 뿌리혹수와 토양 내 유충밀도를 조사한 결과 패랭이, 원추천인국, 기린초, 아주까리, 지모, 해변아욱, 애기똥풀, 흑참깨, 짚신나물, 큰뱀무, 오이풀, 현삼 등 12종은 당근뿌리혹선충에 거의 감염되지 않았음을 확인하였다. 반면에 더덕은 당근뿌리혹선충 접종밀도가 높을수록 뿌리혹수가 많아지고, 토양 내 유충밀도 역시 높아졌으며, 이에 따라 더덕의 생육은 접종밀도가 높을수록 나빠지는 경향이었다.