• Natural Product Sciences
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• v.27 no.4
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• pp.245-250
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• 2021

The methylglyoxal (MGO) trapping constituents from Malus baccata L. were investigated using incubation of MGO and crude extract under physiological conditions followed by HPLC analysis. The peak areas of MGO trapping compounds decreased, and their chemical structures were identified by HPLC-ESI/MS. Sieboldin was identified as a major active molecule representing MGO-trapping activity of the crude extract. After reaction of sieboldin and MGO, remaining MGO was calculated by microplate assay method using imine (Schiff base) formation of 2,4-dinitrophenylhydrazine (DNPH) and aldehyde group. After 4 h incubation, sieboldin trapped over 43.8% MGO at a concentration of 0.33 mM and showed MGO scavenging activity with an RC₅₀ value of 0.88 mM for the incubation of 30 min under physiological conditions. It was also confirmed that sieboldin inhibited the production of advanced glycation end products (AGE) produced by bovine serum albumins (BSA)/MGO. Additionally, MGO trapping mechanism of sieboldin was more specifically identified by ¹H-, ¹³C-, 2D NMR and, confirm to be attached to the position of C-3' (or 5').

• Journal of Korean Society of Forest Science
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• v.90 no.4
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• pp.548-557
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• 2001

This study was carried out to classify forest vegetation of Mt. Myeonsan with phytosociological analysis of ZM School and to explain community types with CCA ordination. The research sites were located in the northern part of Kyungsangpook-Do area and in the southern part of Kangwon-Do. The 73 plots were investigated in the study areas which were including 291 plant species. The forest vegetation was classified into Quercus mongolica community group, Juglans mandshurica community group, and Larix leptolepis plantation. Q. mongolica community group was divided into 9 communities such as Rhododendron schlippenbachii community, Symplocos paniculata community, Pinus densiflora community, Betula schmidtii community, Fraxinus rhynchophylla community, Populus davidiana community, Tilia amurensis community, Betula costata community and Betula davurica community. J. mandshurica community group was divided into 5 communities and 4 groups such as Acer mono community (subdivided into Quercus serrata group and typical group), Fraxinus mandshurica community, Ulmus laciniata community (subdivided into typical group and Ribes mandshuricum group), Malus baccata community and Carpinus laxiflora community. And it was entirely classified into 17 vegetation units. According to CCA, Q. mongolica community group showed high positive correlation to topography, altitude and slope degree, and J. mandshurica community group showed high positive correlation to bare rock. However, L. leptolepis community group was not significantly ordinated with the gradient of environmental factors. And in correlation of axes, important environmental variables were topography and altitude.