• Korean Journal of Food Science and Technology
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• v.20 no.1
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• pp.52-58
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• 1988

This study has investigated the effect of Ganoderma lucidum water soluble extract on higher alcohol production of Saccharomyces cerevisiae. S. cerevisiae was inoculated in malt wort media which were added 0, 0.01, 0.1, 0.5 or 1.0% extracts of G. lucidum and fermented at $15^{\circ}C$ for 120 hours respectively. Some results about cell growth, changes of pH and sugarity and alcohol composition during fermentation are as follows; The cell growth of S. cerevisiae was remarkablely increased as G. lucidum extract was added into each step. It was increased to 1.2, 1.5, 1.9 times on added group of 0.1%, 0.5%, 1.0% G. lucidum extract than control group after the fermentation of 120 hours. Content of sugar was systematically low on step of addition of G. lucidum extract after the fermentation of 120 hours but pH was systematically high. The more the quantity of G. lucidum extract was added, the more total alcohol quantity were produced during fermentation. Especially higher alcohol content was more increased about 1.8 times on 1.0% group (219.9 ppm w/v) than on control group (117.26 ppm w/v) after the fermentation of 120 hours.

• Korean Chemical Engineering Research
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• v.53 no.4
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• pp.524-529
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• 2015

This study was conducted to optimize the medium composition for cold-adaptive protease production of Enterobacteriaceae sp. by response surface methodology (RSM). Yeast extract, and TritonX-100 were identified as the significant factors affecting protease from one-factor-at-a-time method. RSM studies for optimizing protease production of Enterobacteriaceae sp. have been carried out for three parameters including yeast extract concentration, TritonX-100 concentration, and culture pH. These significant factors were optimized as 6.690 g/L yeast extract, 0.018 g/L Triton$^{TM}$ X-10, and pH 6.677. The experimentally obtained protease activity was 8.03 U /L, and it became 1.5-fold increase before optimization.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.369-376
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• 1997

These researches were carried out for improvement of medium for mycelial growth of Hericium erinaceus isolate KU-1. It grew well at pH 4 and $25^{\circ}C$. Glucose and sucrose were favorable carbon sources for mycelial growth. As nitrogen sources, ammonium acetate and arginine enhanced mycelial growth. Optimum C/N ratio was 200. Based On the results, the following recipe is suggested for synthetic medium for the mycelial growth: glucose 18.02 g, arginine 2.613 g, ammonium acetate 2.313 g, $CaCl_2\;0.33\;g$, $KH_2PO_4\;8.5\;g$, $MgSO_4{\cdot}7H_2O\;2.0\;g$, $FeSO_4{\cdot}7H_2O\;0.02\;g$, $ZnSO_4{cdot}7H_2O\;0.02\;g$, $MnSO_4{\cdot}7H_2O\;0.02\;g$, water 1 liter. This medium was superior for the mycelial growth to other conventional media such as Yeast malt extract agar (YMA), Park medium, Potato dextrose agar (PDA), Malt extract agar, Czapek-dox agar, Macaya-lizano medium and Yeast extract agar. This new synthetic medium is designated as Ko medium.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1507-1514
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• 2008

Cholesterol oxidase catalyses the conversion of cholesterol to 4-cholesten-3-one. This enzyme has been used for clinical assay of human serum cholesterol and for reduction of cholesterol level in foods and feeds. In order to search the microorganism which has a high extracellular and stable activity of cholesterol oxidase, soil microorganisms were screened. As a result, the one with the highest extracellular cholesterol oxidase activity was obtained and named as the BEN 115. The BEN 115 strain was identified as one of the Nocardia species based on our taxonomic studies. The cholesterol oxidase from this strain was shown to have two bands of extracellular proteins on SDS-PAGE and Western blot. Their molecular masses were estimated to be about 55 and 57 kDa, respectively. In addition, this cholesterol oxidase was considerably stable at the broad range of pH $3.5{\sim}9.5$ and at the temperature of $25{\sim}55^{\circ}C$. The optimum pH and temperature of this cholesterol oxidase were pH 5.5 and $35^{\circ}C$, respectively. The activity of extracellular cholesterol oxidase could be enhanced 1.6 to 2.0 folds by the addition of nonionic detergent such as Triton X-114, Triton X-100, or Tween-80 into the culturing broth. The substrate specificities against campesterol, sitosterol and stigmasterol were measured to be 50%, 50%, and 27%, respectively, compared to the cholesterol. These results suggest that Nocardia sp. BEN 115 may be useful as a microbial source of cholesterol oxidase production.

• The Korean Journal of Mycology
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• v.25 no.3 s.82
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• pp.226-232
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• 1997

The mycelium was isolated from the fruiting body of Tricholoma matsutake collected from Mt. Namsan, Kyongju and it was named as Tricholoma matsutake DGUM 26001. For the mycelial growth of T. matsutake DGUM 26001, the complex media, yeast-malt extract medium and Czapek-Dox medium supplemented with yeast extract, were excellent. The media such as nutrient glucose medium, mushroom complex medium, and Tricholoma matsutake medium (TMM), were effective. However, There was no a mycelial growth in the media used for bacterial cultivation such as colombia medium, brain heart infusion medium, Luria-Bertani medium supplemented with glucose, and brucella medium. When carbohydrate as a carbon and energy source was supplemented in the TMM medium for the mycelial growth, starch as a polysaccharide was best. As a disaccharide, trehalose and maltose were excellent. Sorbitol, xylitol and glucose were excellent carbon sources of monosaccharose. When the mycelia were cultivated for 30 days at $24^{\circ}C$ in the TMM supplemented with 2.0% starch, the dry weight of the mycelia harvested was 8.85 g/L. When organic acid was given as a carbon source, only succinic acid was utilized. As a vitamin source, coconut water and pyridoxine were excellent. After 30 day-cultivation in the TMM medium, the dry weights with coconut water and pyridoxine were 8.65 and 8.32 g/L, respectively.

• Journal of Microbiology and Biotechnology
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• v.31 no.5
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• pp.717-725
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• 2021

This study aimed to optimize medium composition and culture conditions for enhancing the biomass of Lactobacillus plantarum 200655 using statistical methods. The one-factor-at-a-time (OFAT) method was used to screen the six carbon sources (glucose, sucrose, maltose, fructose, lactose, and galactose) and six nitrogen sources (peptone, tryptone, soytone, yeast extract, beef extract, and malt extract). Based on the OFAT results, six factors were selected for the Plackett-Burman design (PBD) to evaluate whether the variables had significant effects on the biomass. Maltose, yeast extract, and soytone were assessed as critical factors and therefore applied to response surface methodology (RSM). The optimal medium composition by RSM was composed of 31.29 g/l maltose, 30.27 g/l yeast extract, 39.43 g/l soytone, 5 g/l sodium acetate, 2 g/l K₂HPO₄, 1 g/l Tween 80, 0.1 g/l MgSO₄·7H₂O, and 0.05 g/l MnSO₄·H₂O, and the maximum biomass was predicted to be 3.951 g/l. Under the optimized medium, the biomass of L. plantarum 200655 was 3.845 g/l, which was similar to the predicted value and 1.58-fold higher than that of the unoptimized medium (2.429 g/l). Furthermore, the biomass increased to 4.505 g/l under optimized cultivation conditions. For lab-scale bioreactor validation, batch fermentation was conducted with a 5-L bioreactor containing 3.5 L of optimized medium. As a result, the highest yield of biomass (5.866 g/l) was obtained after 18 h of incubation at 30℃, pH 6.5, and 200 rpm. In conclusion, mass production by L. plantarum 200655 could be enhanced to obtain higher yields than that in MRS medium

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.174-174
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• 1994

항종양 물질의 탐색 및 연구 작업이 미생물을 대상으로 행해져 온것은 이미 오래전부터 이며, 기존의 연구 발표된 것들을 볼때 미생물 스크리닝의 증요성은 점차 증대되어가고 있다. 이에 본 연구에서는 국내 토양에서 방선균 약 1000여주를 분리하여 항종양 물질을 생산하는 방선군의 분리, 동전하고, 그 생산물질의 특성을 밝히고자 하였다. 방선균의 분리는 전국 각지에선 채집한 토양시료 각 1 g 씩을 80 $^{\circ}C$에서 90분간 건조처리한후 멸균된 증류수를 이용하여 각 단계별로 희석하여 Starch nitrate casein agar와 Glycerol asparagine agar를 사용하여 방선균을 분리하였다. 분리된 방선균을 Yeast extract-malt extract 액체 배지에서 7일간(160 rpm/28 $^{\circ}C$) 배양하였다. 여기에서 얻어진 배양액을 이용하여 항종양능을 확인한기 위해서 암세포주인 L1210주와 P388 D$_1$주, 정상 세포주로는 Vero 세포주를 이용한 MTT colorimetric assay를 이용하였다. 이들 실현에 대한 결과로써 S-104, 117, 409의 세 균주의 항종양물질 생산 균주를 분리하였다. 이들 균주가 생산하는 물질들은 기존의 시판 항암제인 Adriamycin에 비해 높게 나타났으며, 이들 균주의 동정은 ISP의 기준에 의하여 행하였다.

• Journal of Environmental Health Sciences
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• v.31 no.1
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• pp.31-38
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• 2005

An anti-fungal material produced by actinomycetes was isolated from domestic soil. This actinomycetes was identified as Streptomyces albogriseus by 16S rDNA sequence. YEME (yeast extract 4 g, malt extract 10 g, glucose 4 g, D.W 1l, pH 7.00.2) medium was used for production of anti-fungal materials. S. albogriseus was cultured in a shaking incubator for 2 weeks at 150 rpm and $25^{\circ}C$. An anti-fungal material produced by S. albogriseus was identified at 340 nm by uv/vis- spectrometer and it showed powerful anti-fungal activity. This is the first report that secondary metabolite produced by S. albogriseus showed an activity against phytopathogenic fungi such as Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.87-89
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• 2008

A blue mold on tomato fruit caused by Penicillium oxalicum occurred sporadically in a greenhouse at Gyeongsangnam-do Agricultural Research and Extension Services. Infection usually occurred through wounds or cracks on the fruits. Symptoms began with water soaking lesions, then became watery and softened eventually. Colony of the causal fungus was white at the early growing stage, turned green on Czapek yeast extract agar and malt extract agar. Conidia were ellipsoidal in shape and $2-6{\times}2-4{\mu}m$ in size. Stipes were septate, smooth, thin walled, and $90-280{\times}3-4{\mu}m$ in size. Penicilli were mostly biverticillate. Ramuli were 1-3 groups, smooth, and $10-16{\times}2-3{\mu}m$ in size. Rami were 1-2 groups and $6-30{\times}2-4{\mu}m$ in size. Metulae were 2-3(5) verticils, smooth, and $12-20{\times}3-4{\mu}m$ in size. Phialides were 5-7 verticilate, ampulliform to cylindroidal, smooth, and $8-12{\times}2-3{\mu}m$ in size. Optimum temperature for growth was about $25^{\circ}C$. Pathogenicity of the fungus was proved on tomato fruit according to Koch's postulation. On the basis of mycological and pathological characteristics, the fungus was identified as P. oxalicum Currie & Thom. This is the first report of the blue mold on tomato fruit caused by P. oxalicum in Korea.

• Journal of Ginseng Research
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• v.6 no.1
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• pp.17-24
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• 1982

This studies were conducted to investigate the changes of saponin pattern, pH and organic acid contents of malt wort added ginseng components during alcoholic fermentation by Sacch. uvarum. The results are as follows. Saponin patterns of fermented wort were same as that of the non- fermented wort, but the weight of former was decreased comparing to that of the latter. pH value of fermented wort contained 0.1∼0.5% of ginseng extract were almost same as that of control(PH 4.23). Lactate, pyruvate, succinate and fumarate, pyroglutarate and citrate contents of the fermented wort were increased by the addition of ginseng extract and pyruvate content, particularly, was increased from 28.4 to 214mg/100 ml while that of control was 33.2mg/100m1. Citrate content of fermented wort contained ginseng saponin was almost same as control (37. 5mg/100m1) . But pyruvate content was tower 4-8.6mg/100m1 than that of control(33.2mg/100m1) .