• Title/Summary/Keyword: Mali

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Genetic polymorphism of merozoite surface protein 1 and antifolate-resistant genes in Plasmodium falciparum from Mali and Niger

  • Mahaman Moustapha Lamine;Rabia Maman;Abdoul Aziz Maiga;Ibrahim Maman Laminou
    • Parasites, Hosts and Diseases
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    • v.61 no.4
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    • pp.455-462
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    • 2023
  • Since 2015, countries in the Sahel region have implemented large-scale seasonal malaria chemoprevention (SMC). However, the mass use of sulfadoxine-pyrimethamine (SP) plus amodiaquine impacts the genetic diversity of malaria parasites and their sensitivity to antimalarials. This study aimed to describe and compare the genetic diversity and SP resistance of Plasmodium falciparum strains in Mali and Niger. We collected 400 blood samples in Mali and Niger from children aged 3-59 months suspected of malaria. Of them, 201 tested positive (Niger, 111, 55.2%; Mali, 90, 44.8%). Polymorphism of merozoite surface protein 1 (msp1) genetic marker showed 201 allotypes. The frequency of the RO33 allotype was significantly higher in Niger (63.6%) than in Mali (39.3%). There was no significant difference in the frequency of the K1 and MAD20 allotypes between the 2 countries. The multiplicity of infection was 2 allotypes per patient in Mali and one allotype per patient in Niger. The prevalence of strains with the triple mutants Pfdhfr51I/Pfdhfr59R/Pfdhps436A/F/H and Pfdhfr51I/Pfdhfr59R/Pfdhps437G was 18.1% and 30.2%, respectively, and 7.7% carried the quadruple mutant Pfdhfr51I/Pfdhfr59R/Pfdhps436A/F/H/Pfdhps437G. Despite the significant genetic diversity of parasite populations, the level of SP resistance was comparable between Mali and Niger. The frequency of mutations conferring resistance to SP still allows its effective use in intermittent preventive treatment in pregnant women and in SMC.

Studies on the Canker of Apple Trees (Causal Organisms and their Chemical Control) (사과나무 부란성병해(부란병, 동고병, 동부병)에 관한 연구 (제2보)병원균의 분포 및 몇 가지 살균제의 방제효과)

  • 원창남;김승철;한정길
    • Korean journal of applied entomology
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    • v.11 no.1
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    • pp.19-23
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    • 1972
  • Apple cankers caused by Valsa mali Miyabe, Botryosphaeria ribis Cross, Phomopsis truncicola Miura, are distributed in the main apple growing areas, Choong Chung Puk Do, Choong Chung Nam Do, and Kyung Sang Puk Do. According to the survey in 1970 that Valid mali is the dominant causal organism among the three in the most severely damaged area. Valsa mali and Botryosphaeria ribis were found in ail 3 provinces, however, Phomopsis truncicola was not found in Choong Chung Nam Do. Especially, Botryosphaeria ribis was most abundant in Chunwon of Choong Chung Nam Do. Effects of chemical control of apple canker caused by Valsa mali were tested both on potato dextrose agar with inhibition zones produced by the fungicides and relative growth of assay fungus of peridermis was treated with tile fungicides. Ifost effective was mercuric and followed by Lime sulfur, Hydroquinone sulfate, in general.

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Description of Fungus Gnat, Lycoriella mali Fitch (Diptera: Sciaridae) from Korea (버섯해충 Lycoriella Mali (긴수염버섯파리: 신칭)에 관한 보고)

  • Heung-Su Lee;Kyu-Chin Kim;Chung-Gyoo Park;Won-Kyo Shin
    • Korean journal of applied entomology
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    • v.38 no.3
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    • pp.209-212
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    • 1999
  • A species of fungus gnat collected from mushroom house was identified as Lycoriella mali Fitch. Morphological characters of this species ar described and briefly compared with other associated species infesting mushrooms.

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Biological Control of Lycariella magi(Diptera: Sciaridae), a Pest of Oyster Mushroom, Pleurotus ostreatus Using Entomopathogenic Nematodes (곤충병원성 선충을 이용한 느타리버섯해충, 긴수염버섯파리 (Lycoriella mali)의 생물적방제)

  • 김형환;추호렬;이흥수;박정규;이동운;진병래;추영무
    • Korean journal of applied entomology
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    • v.40 no.1
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    • pp.59-67
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    • 2001
  • The potential of two entomopathogenic nematodes, Sreinernema carpocapsae Pocheon strain and Heterorhabditis bacteriophora Hamyang strain as biological control agents was evaluated against mushroom ny, Lycoriella mali in laboratory and field. Mortality of L. mali was significantly different according to nematode species, concentration, temperature, and developmental stage of fly S. carpocapsae was more effective than H. bacteriophora. Mortality of L. mali was higher at $25^{\circ}C$ than at $20^{\circ}C$. In addition, the 3rd instal and the 4th instar of L. mali were more susceptible than the 2nd instar. The lowest $LC^{50}$ value was represented by S. carpocapsae, 20.0 infective juveniles (Ijs) in the 3rd instar, 27.5 Ijs in the 4th instar at $25^{\circ}C$. S. carpocapsae infected all the developmental stages of L. mali except egg stage and the 1st instar of larva. The highest mortality was shown in adult female representing 74.0% at$20^{\circ}C$ and 80.0% at $25^{\circ}C$.L. mali female adult was influenced by S. carpocapsae in oviposition. The number of eggs by L. mali female infected by nematodes was much lower than uninfected females. S. carpocapsae was dispersed by infected L. mali adult with higher numbers by females than males. When S. carpocapsae was applied at the rate of $2.25{\times}10^{5}\;and\;4.5{\times}10^{5}\;Ijs/1.5\;\textrm{m}^2$ in the mushroom house, mortalities were 42.2% and 81.6%, respectively. The infective juveniles of nematodes survived for 14 days in the mushroom medium. However, nematodes did not affect mushroom growth.

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Furfural from Pine Needle Extract Inhibits the Growth of a Plant Pathogenic Fungus, Alternaria mali

  • Jung, Kyung-Hwan;Yoo, Sun-Kyun;Moon, Sung-Kwon;Lee, Ung-Soo
    • Mycobiology
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    • v.35 no.1
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    • pp.39-43
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    • 2007
  • The antifungal effect of pine needle extract prepared by a distinguishable extraction method and the dry distillation method, was examined. The effect of this extract itself was insignificant. The chemical components of pine needle extract were then investigated by gas chromatographic analysis, and four, chemical components, acetol, furfural, 5-methyl furfural, and terpine4-ol, were identified. The antifungal effects of those four chemical components against Alternaria mali (A. mali), an agent of Alternaria blotch of apple, were then examined. It was observed that the minimum inhibitory concentrations (MICs) were 6.25, 0.78, 0.78, and 12.5 (mg/ml) of acetol, furfural, 5-methyl furfural, and terpine-4-ol, respectively. MICs of furfural and 5-methyl furfural had the same order of magnitude as that of an antifungal agrochemical, chlorothalonil. Although furfural itself can not be completely substituted for an antifungal agrochemical, a partial mixture of furfural and antifungal agrochemical may be used as a substitute. The use of agrochemicals for the prevention of plant disease caused by pathogenic fungus such as A. mali could be partially reduced by the application of this mixture.

The Aspects and Prospects of Terrorist Group Activities in the Sahel Area through the Changes in the Situation in Mali (말리 내 정세분석을 통해 보는 사헬지대 테러집단 활동 양상과 시사점)

  • SangJun Park
    • Journal of the Society of Disaster Information
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    • v.19 no.2
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    • pp.326-333
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    • 2023
  • Purpose: By analyzing how the Mali conflict has progressed over the past decade, the activities of terrorist groups in the Sahel are predicted, and their movements will have implications. Method: In addition to existing study, progress is identified through the media. Result: The Mali conflict has been sustained by the spread of ethnic conflict, Islamic extremism, and military coups. Conclusion: The influence of the international community is being weakened with the withdrawal of France and the intervention of Russia. This indicates that the Mali conflict could be prolonged.

Mycological Characteristics and Pathogenicity of Phomopsis mali Causing Fruit Decays of Japanese Apricot, Apple and Kiwifruit (매실, 사과 및 참대래의 과실썩음병을 일으키는 Phomopsis mali의 균학적 특징과 병원성)

  • 이정혜;이두형
    • Korean Journal Plant Pathology
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    • v.14 no.2
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    • pp.109-114
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    • 1998
  • To investigate Phomopsis species causing fruit decays of Japanese apricot, apple and kiwifruit, we collected diseased fruits from the fruit markets in 1995 and 1996 respectively. Phomopsis mali Roberts was identified based on cultural characteristics, morphological aspects and pathogenicity. There were no remarkable differences with respect to $\alpha$ and $\beta$ conidia, growth rates and colony characters among the isolates from Japanese apricot, apple and kiwifruit. The pathogens grew more than 70 mm on potato dextrose agar in 5 days at $25^{\circ}C$. The agar was slightly discolored by the production of a reddish purple pigment under the light at $25^{\circ}C$ and 3$0^{\circ}C$ respectively. Only $\alpha$ spores of the different isolates of P. mali were formed at 15$^{\circ}C$ and $\beta$ spores were mainly produced at 3$0^{\circ}C$, but and $\alpha$ and $\beta$ spores were produced in approximately equal numbers at 2$0^{\circ}C$ and $25^{\circ}C$. Pycnidia were a few under the dark condition but were abundant at wide range of 15~3$0^{\circ}C$ under near ultra violet illumination. Conidia were two types : $\alpha$ spores were unicellar, fusoid, hyaline and biguttulate, whereas $\beta$ sores were unicellar, acicular to filiform, straight or hooked and hyaline. An ascigerous stage was not formed in cultures or in nature. Isolates of Phomopsis mali from japanese apricot, apple and kiwifruit could infect fruits of apple, pear, apricot, Japanese apricot and kiwifruit. There were some differences in pathogenicity depending on stocks of fruit crops tested.

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Effect of Apple Leaf Hair Density on the Occurrence and Infection of Alternaria Leaf Spot Caused by Alternaria mali Roberts (모용(毛茸)이 사과나무 반점낙엽병(斑點落葉病) 발생(發生)과 병원균(病原菌)의 감염(感染)에 미치는 영향(影響))

  • Yoon, J.T.;Lee, J.T.
    • Korean journal of applied entomology
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    • v.26 no.4 s.73
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    • pp.245-249
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    • 1987
  • In the 82 apple varieties tested, the distribution of varieties grouped on the damage degree to Alternaria leaf spot were 15 varieties(l8.3%), which damaged above 50%, and 51 varieties(62.2%) damaged below 5%. Susceptible varieties to Alternaria leaf spot were Raritan, Indo, Winesap and Starkrimson, moderately resistant varieties Sekaiichi and Starking, and resistant Jonathan, Tsugaru, Molliies, Jonagold, Gala etc. The leaf hair density on the backside of leaf were rarer inresistant varieties than susceptible one. In inoculation test, penetration and infection of Alternaria mali were easier on the backside than surface of apple leaf at the same varity, and infection rate with Alternaria mali were higher at removal treatment than non-removal one of leaf hairs, so we can guess that leaf hair are related to resistance of variety to Alternaria mali.

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Selection of insecticides for controlling Lycoriella mali in Letinula edodes sawdust cultivation (표고 톱밥재배에서 긴수염버섯파리 (Lycoriella mali)의 방제약제 선발)

  • Kim, Gil-Hah;Yoo, Jeong-Su;Koo, Chang-Duck;Lee, Sang-Gil;Park, Ji-Doo
    • The Korean Journal of Pesticide Science
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    • v.5 no.2
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    • pp.62-66
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    • 2001
  • The thirteen commercial insecticides to Lycoriella mali were investigated on their insecticidal activities and mycelial growth of two Letinula edodes strains. For the adults, insecticides showing over 95% insecticidal activity were chlorpyrifos-methyl, fenthion, fenitrothion, benfuracarb, furathiocarb and deltamethrin. For tile larvae, diflubenzuron and cyromazine showed over 90% insecticidal activity. Fenthion, benfuracarb, furathiocarb, deltamethrin, diflubenzuron and cyromazine did not affect the mycelial growth of L. edodes strain, in Imhyup 1 variety. And deltamethrin, diflubenzuron and cyromazine did not affect that in Sanlim-5. Insecticides showing over 80% residual effect for 14 days were benfuracarb to the adults and diflubenzuron and cyromazine to the larvae. Control effect of furathiocarb, fenthion, benfuracarb, deltamethrin, diflubenzuron and cyromazine against larvae of Lycoriella mali showed 90% ten days after application under sawdust cultivation. Among them, benfuracarb, fenthion and furathiocarb revealed excellent control effect against adults. These results indicate that benfuracarb, fenthion, and furathiocarb can be used for the control of Lycoriella mali in the field. However, further studies are needed on the effect of insecticides treatment oil fruit-body yield and chemical residue in the mushroom tissues.

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