• 국제학술발표논문집
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• The 5th International Conference on Construction Engineering and Project Management
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• pp.80-86
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• 2013

Knotworking represents a distributed collaborative expertise in pursuit of a task that is organized among designers from different design disciplines. Construction processes involve phases and tasks that cannot be solved in one organization only, as integration of expert knowledge from various sources is needed. Through knotworking, groups of people, tasks and tools are set to work intensively for a short period of time to solve a problem or accomplish a task. Knotworking requires intensive collaboration across organizational boundaries and hierarchies. The practice of knotworking has been developed and applied in the development of healthcare organizations, libraries and school-university relationships, but it has not previously been applied in the construction industry. In this paper, we describe the concept of knotworking and the findings of a case study that we completed in the Finnish construction industry. We will also compare the similarities and differences of the Big Room and knotworking in terms of participants, duration, target, space/infrastructure, benefits and challenges. Finally, we present some suggestions for further research and experimentation on knotworking in construction projects.

• Journal of Photoscience
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• 제9권2호
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• pp.308-310
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• 2002

Bacteriorhodopsin (bR) and halorhodopsin (hR), which exist in the membrane of Halobacterium salinarum, are light-driven ion pumps. In spite of high similarity of primary and tertiary structures between bR and hR, these membrane proteins transport different ions, proton and chloride, in the opposite direction. From alignment of the amino acid sequences, Thr-89 of bR is homologous to Ser-l15 of hR from Halobacterium salinarum (shR). X-ray structure of shR has revealed that OH group of this residue directly interacts with CI$\^$-/ Thus, Ser-lI5 of shR is expected to play an important role in CI$\^$-/ binding and transport. In this study, we expressed wild type hR from Natronobacterium pharaonis (PhR) and Sl30A, which corresponds to Ser-l15 of shR, in E. coli in order to clarify binding affinity of chloride ion and photocycle reactions. From the titration with CI$\^$-/, affinity of Sl30A became quite lower than that of WT (WT 6 mM, Sl30A 89 mM). Furthermore, from the flash photolysis with pulse laser of λ$\_$max/ at 532 nm, the reaction rate of SI30A from 0 intermediate to hR ground state was found to become apparently slower than that of WT. The singular value decomposition (SVD) and global fitting analyses of the photocycles were performed to identify all photointermediates and determine the reaction rates.

• Toxicological Research
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• 제14권2호
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• pp.123-127
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• 1998

BRCA1 is a tumor suppresser gene in familial cases of breast cancer. It has been controversial whether the subcellular localization of BRCA1 is located in nuclei or cytoplasm in normal human breast cells. We found that a p220 protein was expressed in Type II Normal human breast epithelial cells (NHBEC) but not in Type I NHBEC in Western blot analysis using the 17F8 (3A2) antibody. Immunostaining using the same antibody revealed positive staining in nuclei, cytoplasm and perinuclei of Type II cells and negative staining in Type I NHBEC. The p220 protein, however, was expressed in SV40 immortalized Type I NHBEC and tumorigenic cells derived from them after x-ray and neu oncogene treatment. The subcelluar localization was mostly cytoplasmic and punctate in the nuclei. The breast carcinoma cell lines, MCF-7 and T47D, also expressed the p220 protein. Using RT-PCR, we observed the expression of BRCA1 mRNA in both Type I and Type II NHBEC. This result indicated that there might be mechanisms involved in post-translational or translational regulation of BRCA1 gene. It is speculated that the absence of BRCA1 protein expression in Type I NHBEC might playa role in their susceptibility to neoplastic transformation.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 ITC-CSCC -1
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• pp.490-493
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• 2002

In this paper, the old Fourier-Motzkin method (abbreviated as the old FH method from now on) is first modified to the form which can derive all minimal vectors as well as all minimal support vectors of nonnegative integer homogeneous solutions (i.e., T-invariants) for a matrix equation $Ax=b=0^{m{\times}1}$, $A\epsilonZ^{m{\times}n}$ and $b\epsilonZ^{m{\times}1}$, of a given Petri net, where the old FM method is a well-known and direct method that can obtain at least all minimal support solutions for $Ax=0^{m{\times}1}$ from the incidence matrix . $A\epsilonZ^{m{\times}n}$, Secondly, for $Ax=b\ne0^{m{\times}n}$ a new extended FM method is given; i.e., all nonnegative integer minimal vectors which contain all minimal support vectors of not only homogeneous but also inhomogeneous solutions are systematically obtained by applying the above modified FH method to the augmented incidence matrix $\tilde{A}$ =〔A,-b〕$\epsilon$ $Z^{m{\times}(n＋1)}$ s.t. $\tilde{A}\tilde{x}$ = 0^{m{\times}1}$ However, note that for this extended FM method we need some criteria to obtain a minimal vector as well as a minimal support vector from both of nonnegative integer homogeneous and inhomogeneous solutions for Ax=b. Then those criteria are also discussed and given in this paper.

• 대기
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• 제17권4호
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• pp.407-420
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• 2007

Optical characteristics of aerosols in Seoul are investigated from the measurements of sky radiance by Skyradiometer at Yonsei University from December 2005 to November 2006. Aerosol optical depth (AOD) shows a maximum in June due to weak ventilation and particle growth by aging process and hygroscopic effect. Single scattering albedo (SSA) and Angstrom Exponent (AE) show the lowest value in spring due to the Asian dust. It is clear that coarse mode is dominant in spring and fine mode is dominant in summer from the volume size distribution measured in this study. The explanations on the changes of aerosol loadings are provided through the correlation between AOD and AE, while the pattern of wavelength dependency related to particle size is shown through the correlation between SSA and AE. Backward trajectory analysis by HYSPLIT provides information about origin of aerosol, which allows us to classify the case according to the source region and the path distance. Although the direction of backward trajectory traces back mostly to west, coarse mode particle is dominant in the case of long pathway and fine mode particle is dominant in the case of short pathway. This discrepancy is caused by the regional difference of emitted particles.

• Asian Journal of Atmospheric Environment
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• 제5권3호
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• pp.164-171
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• 2011

This work focuses on the analysis of bioaerosols in the atmosphere at higher altitudes over Noto Peninsula, Japan. We carried out direct sampling via aircraft, separated cultures, and identified present isolates. Atmospheric bioaerosols at higher altitudes were collected using a Cessna 404 aircraft for an hour at an altitude of 3,500 m over the Noto Peninsula. The aircraft-based direct sampling system was devised to improve upon the system of balloon-based sampling. In order to examine pre-existing microorganism contamination on the surface of the aircraft body, bioaerosol sampling was carried out just before takeoff using the same method as atmospheric sampling. Identification was carried out by a homology search for 16S or 18S rDNA isolate sequences in DNA databases (GenBank). Isolate sampling just before takeoff revealed Stretpomyces sp., Micrococcus sp., and Cladosporium sp. One additional strain, Bacillus sp., was isolated from the sample after bioaerosol collection at high altitude. As the microorganism contamination on the aircraft body before takeoff differed from that while in the air, the presence of additional, higher atmosphere-based microorganisms was confirmed. It was found that Bacillus sp. was floating at an altitude of 3,500 m over Noto Peninsula.

• Asian Journal of Atmospheric Environment
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• 제5권3호
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• pp.157-163
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• 2011

Atmospheric aerosol deposition caused by Asian dust (KOSA) events provide nutrients, trace metals, and organic compounds over the Pacific Ocean that enhance ocean productivity and carbon sequestration and, thus, influence the atmospheric carbon dioxide concentrations and climate. Using dust particles obtained from the snow layers on Mt. Tateyama and the surface sand of Loess Plateau in incubation experiments with natural seawater samples on a shipboard, we demonstrate that dust-particle additions enhanced the bacterial growth on the first day of incubation. Gram-positive bacterial group and alpha-proteobacteria were specifically detected form seawater samples including the mineral particles. Although the remarkable dynamics of trace elements and nutrients depend on dust-particle additions, it is possible that organic compounds present in the mineral particles or transported microbial cells could also contribute to an increase in the quantities of bacteria. The chlorophyll concentrations at fractions of every size indicated a similar pattern of change between the seawater samples with and without the dust-particle additions. In contrast, the chlorophyll measurement using submersible fluorometer revealed that the dynamics of phytoplankton composition were influenced by the dust-particles treatments. We conclude that the phytoplankton that uses the bacterial products would increase their biomass. We show that KOSA deposition can potentially alter the structures of bacterial communities and indirectly influence the patterns of marine primary production in the Pacific Ocean.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.891-896
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• 2007

We screened the Arabidopsis cDNA library to identify functional suppressors of AtBI-1, a gene that suppresses cell death induced by Bax gene expression in yeast. Cdf 3 encodes a 118-amino-acid protein with a molecular mass of 25 kDa. This protein has two uncharacterized domains at amino acids residues 5-64 and 74-117. In the present study, CDF3 was found to induce growth defects in yeast and arrested yeast growth, although the cell-growth defect was somewhat less than that of Bax. Its localization in the inner mitochondria was essential for suppression of yeast-cell proliferation. The morphological abnormality of the intracellular network, which is a hallmark of AtBI-1, was attenuated by Cdf3 expression.

• 천문학회지
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• 제29권spc1호
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• pp.333-335
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• 1996

Here we report the results from spectroscopic observations of soloar active regions in the HeI 10830 ${\AA}$ line at the German Vacuum Tower Telescope(VTT) in Tenerife during the August 199:3 International EFR(Emerging Flux Region) Campaign. Four active regions in various stages of their evolution, i.e., NOAA7558, 7560, 7561, and 7562, were ovserved on 10 August 1993. From the observed HeI 10830 ${\AA}$ spectra in these active regions, spectroscopic quantities such as equivalent width(EW), doppler shift, doppler width, etc., were derived(see Figure l(a)) and the correlation between them were studied(see Figure l(b)). Our main results are as follows: (I)In NOAA7562, which is a young and evolving EFR, the EW is large, while it is small around a simple and roundish spot of NOAA7558. (2)In these active regions, redshift in the 10830 line is dominant when the EW is larger. (3)As the doppler width increases, the line tends to shift redward. (4)When the EW is smaller, it seems to exist another component which have dynamic characteristics different from the redshifting component. In NOAA7560 and NOAA7561, regions which have several small spots, the values of the EW are intermediate. Results (2) and (3) may suggest the possible existence of downflow above active regions, if the HeI 10830 ${\AA}$line is formed in the upper chromopshere, and it is consistent with the earlyer result from the SMM extreme-ultraviolet observation by Klimchuk(1987, Astrophys. J., 323, 368) (to be submitted. to Astronomy and Astrophysics; an extended abstract)

• BMB Reports
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• 제47권5호
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• pp.286-291
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• 2014

Inoculation of mice with the murine NFSA cell line caused the formation of large tumors with necrotic tumor cores. FACS analysis revealed accumulations of $CD11b^+$ cells in the tumors. Microarray analysis indicated that the NFSA cells expressed a high level of the pro-inflammatory factor interleukin-18 (il-18), which is known to play a critical role in macrophages. However, little is known about the physiological function of IL-18-stimulated macrophages. Here, we provide direct evidence that IL-18 enhances the phagocytosis of RAW264 cells and peritoneal macrophages, accompanied by the increased expression of tumor necrosis factor (tnf-${\alpha}$), interleukin-6 (il-6) and inducible nitric oxide synthase (Nos2). IL-18-stimulated RAW264 cells showed an enhanced cytotoxicity to endothelial F-2 cells via direct cell-to-cell interaction and the secretion of soluble mediators. Taken together, our results demonstrate that tumor-derived IL-18 plays an important role in the phagocytosis of macrophages and that IL-18-stimulated macrophages may damage tumor endothelial cells.