• 한국멀티미디어학회논문지
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• 제23권10호
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• pp.1250-1257
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• 2020

The early detection of diseases is important in agriculture because diseases are major threats of reducing crop yield for farmers. The shape and color of plant leaf are changed differently according to the disease. So we can detect and estimate the disease by inspecting the visual feature in leaf. This study presents a vision-based leaf classification method for detecting the diseases of tomato crop. ResNet-50 model was used to extract the visual feature in leaf and classify the disease of tomato crop, since the model showed the higher accuracy than the other ResNet models with different depths. We propose a new ensemble approach using several DCNN classifiers that have the same structure but have been trained at different ranges in the DCNN layers. Experimental result achieved accuracy of 97.19% for PlantVillage dataset. It validates that the proposed method effectively classify the disease of tomato crop.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.401-401
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• 2005

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.207-222
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• 2021

Coffee is the most frequently consumed functional beverage world wide. The average daily coffee consumption is increasing. This crop, which plays an important role in the global economy is under great threat from climate change. To with stand the current climate change, farmers have to learn crop cultivation techniques, strategies to protect crops from diseases, and understand which type of seed varieties to use to avoid crop loss. The present review briefly discusses the coffee cultivation techniques, impact of climate changes on coffee production, processing techniques of coffee, and the importance of coffee in our society, including its chemical composition and prevention against, major diseases. Furthermore, the importance and role of advanced nanotechnology along with molecular approaches for coffee crop improvement and facing challenges are explained.

• 한국약용작물학회지
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• 제1권1호
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• pp.74-80
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• 1993

주산지(主産地)의 약용작물(藥用作物) 재배(栽培) 및 생산(生産) 실태(實態)와 문제점(問題點)을 파악하기 위하여 전국(全國) 66개소에서 초본류(草本類) 18종(種), 목본류(木本類) 5종(種)의 약초(藥草)를 대상으로 조사한 결과는 다음과 같다. 1. 약용작물(藥用作物) 종자(種子)나 종묘(種苗)를 자가채종(自家採種) 또는 인근(隣近) 농가(農家)에서 구입(購入)하는 농가가 전체의 82%로 그 지방(地方)의 재내종(在來種)이 이용되고 있었다. 2. 1시비실태(施肥實態)는 화학비료(化學肥料)보다 유기질비료(有機質肥料) 시용(施用)이 많았으며 이중 퇴비사용농가 60.7%, 유기물(有機物)을 사용하지 않는 농가도 34.8%로 나타냈다. 한편 화학비료별로는 복합비료(復合肥料) 시용(施用)이 51.5%, 요소단용(尿素單用)이 40.9%였다. 3. 주산단지(主産團地)의 약용작물(藥用作物)중 병충해(炳蟲害)에 강한 작물은 미나리과(科)의 강활(羌活), 당귀(當歸), 방풍(防風), 시호(柴浩)와 독활(獨活), 황정(黃精), 두충(杜沖)이었고 주요해충(主要害蟲)은 응애, 진딧물, 조명나방, 굼댕이, 고자리 파리,거세미 등이고 주요병(主要病)은 흰가루병 갈반병, 잎마름병, 뿌썩음병 등이었다. 4. 작물별(作物別) 지역간(地域間) 재배(栽培), 생산(生産)과 소득(所得)은 파종기(播種期), 식재(植栽)거리, 수확년수(收穫年數), 판매방법(販賣方法)에 파라 차이(差異)가 크게 나타났으며 10a당(當) 조수익(祖收益)은 적은 농가 312천원에 비해 많은 농가는 11.3배(倍)의 소득(所得)을 보였다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 춘계학술발표회
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• pp.108-109
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• 2008

• 한국작물학회지
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• 제58권2호
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• pp.142-148
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• 2013

국내 콩에서 발생하는 세균병해인 불마름병, 들불병, 세균점무늬병, 세균갈색점무늬병의 다중 진단을 위한 PCR 방법을 요약하면 다음과 같다. 1. 콩에 발생하는 각각의 세균들은 서로 다른 박테리오신(bacteriocin) 이나 파이토톡신(phytotoxin)을 생산하는데 이와 관련한 유전자를 목적으로 하여 진단프라이머를 설계하였다. 2. 불마름병은 glycinecin A, 들불병은 tabtoxin, 세균점무늬병은 coronatine과 세균갈색점무늬병은 syringopeptin을 목적유전자로 하여 다중 진단프라이머 조합을 설계하였다. 3. 1차 선발로 각각의 균주에 대한 단일 진단 프라이머를 선발하였으며, 여기선 선발된 21개의 프라이머들을 조합하여 4종 다중진단프라이머 선발을 위한 2차 선발에 이용하였다. 최종적으로 280 bp의 불마름병, 355 bp의 세균갈색점무늬병, 563 bp의 들불병과 815 bp의 세균점무늬병으로 구성된 다중진단 프라이머 조합이 개발되었다. 4. 선발된 4종 다중 진단 프라이머 조합의 경우 다른 세균들과의 비특이적 반응이 있는지 확인하기 위한 3차 선발을 거쳐 그 특이성을 검증하였다.

• Plant Breeding and Biotechnology
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• 제7권3호
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• pp.272-286
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• 2019

Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F₁ hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F₁ hybrid combinations and two control vatieties. Results showed that nine F₁ hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F₁ hybrid rice varieties with resistance to disease/insect were selected from eleven F₁ hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F₁ hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.112-112
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• 2017

Rice blast caused by Magnaporthe oryzae is one of the most serious diseases that affect the quantity and quality of rice production. The use of resistant rice varieties would be the most effective way to control the rice blast. However R gene incorporation into the rice variety takes time and pathogen could overcome the R gene effects after for a while. For monitoring the rice blast resistance gene distribution in Korean varieties, the four major blast resistance genes against M. oryzae were screened in a number of Korean rice varieties using molecular markers. Of the 120 rice varieties tested, 40 were found to contain the Pi-5 gene, 25 for the Pi-9 gene, 79 for Pi-b and 40 for the Pi-ta gene. None of these rice varieties includes tested 4 R genes. 3 R genes combination, Pi-5/Pi-9/Pi-b, Pi-5, Pi-9.Pi-ta, or Pi-9/Pi-b/Pi-ta were found in 12 varieties, the rice blast disease severity were showed as resistant in the rice verities containing Pi-9/Pi-b/Pi-ta R genes combination, respectively. Also pathogenic diversity of M. oryzae isolates collected in the rice field from 2004 to 2015 in rice field in Korea were analyzed using rice blast monogenic lines, each harboring a single blast resistance gene. Compatibility of blast isolates against rice blast monogenic lines carrying the resistance genes Pi5, Pi9, Pib, and Piz showed dynamic changes by year. It indicates that pathogen has high evolutionary potential adapted host resistances to increase fitness and would lead to rice blast resistance bred into the cultivar becoming ineffective eventually.

• 한국약용작물학회지
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• 제14권4호
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• pp.202-205
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• 2006

인삼의 안정적인 생산 및 합리적인 병해방제에 기여하기 위하여 연구를 실시한 결과 갈잎 퇴비에서 분리된 Streptomyces lauretii B8180과 Bacillus subtilis B8856, Burkholderia cepacia B7944 세 균주의 인삼병원균 5종에 대한 항균력이 인정되었으며, 특히 실내실험을 통하여 높은 역병 방제효과가 인정되었다. 그러나 이 실험에서와 같이 식물의 지상부에 길항균을 살포하는 방법을 통하여 역병 방제효과를 높일 수 있을 것으로 생각된다. 이 길항균들은 갈잎 퇴비에서 분리되었기 때문에, 갈잎 퇴비에서 증식시켜 인삼재배 예정지에 투입하면, 억제형 토양 (suppressive soil) 조성을 통한 주요병해 방제가능성도 클 것으로 생각된다.

• 농업과학연구
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• 제43권2호
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• pp.153-162
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• 2016

Chilli is a widely consumed crop throughout the world. However, chilli anthracnose is a major constraint in chilli production leading to huge economic losses worldwide. Colletotrichum is a large genus of Ascomycete fungi, containing species that cause anthracnose diseases on a wide range of crops of economic value. This review is aimed at critically and accurately examining the taxonomic identification of Colletotrichum species by morphological and molecular approaches as well as assessing their management options. The use of appropriate integrated management practices, such as cultural, mechanical, chemical, and biological control, are important in chilli anthracnose disease prevention and control. Emphasis is laid on the use of biological control because it is cost effective and eco-friendly, and is an appropriate approach for disease management. The use of resistant cultivars is the cheapest, easiest, safest, and most effective means of controlling crop diseases. But, since no resistant cultivars of chilli have been developed and commercialized, it is very important to develop biological management strategies. Further studies leading to integrated disease management strategies need to be carried out.