• 설비공학논문집
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• 제17권2호
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• pp.125-130
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• 2005

This study was peformed to investigate the phase change temperature, the supercooling, the maintenance time of liquid phase and the change rate of volume of TMA 30 $wt\% clathrate compound with additives. TMA 30 $wt\% clathrate compound with additive was cooled at heat source temperature of $-6^{circ}C$. The additives are ethylene glycol and chloroform. Their concentration are 0.1$wt\% respectively. The experimental results showed that the phase change temperature was not affected by additives and this was average $5.3^{circ}C$. Also the supercooling and the maintenance time of liquid phase were decreased by additives. Especially, the average value of supercooling showed by $8.8^{circ}C$ and the maintenance time of liquid phase was by 19 minutes in the case of chloroform 0.1$wt\%. Additionally, the average change rate of volume showed by $1.26{\~}1.31\%$ according to additives and the volume was decreased by the phase change from liquid to solid.

• 한국구조물진단유지관리공학회 논문집
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• 제8권4호
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• pp.87-96
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• 2004

소일시멘트는 자연 상태의 흙을 주원료로 시멘트, 혼합수를 배합하여 일반포장용 콘크리트에 준하는 강도를 발휘하는 특수콘크리트로서 최근 도로포장뿐만 아니라 연악지반 개량 동 여러 분야에 활용되고 있다. 본 연구에서는 화강토 및 붕적토에 고화제를 첨가하여 일축압축강도의 특성 및 시간의존 거동을 규명하고자 하였다 연구 결과 일축압축강도는 시멘트 함량의 증가와 양생기간의 증가에 따라 뚜렷한 강도 증가를 타나내었으며 미 세립분이 많을 수 록 강도가 감소하였으며 X-선 회절 분석 결과 소일시멘트에서 반응생성물인 Vermiculite가 발견되었다 동적반복재하크리프 시험 결과 히스테리시스 곡선으로부터 재료의 동적특성인 복합전단컴플라이언스, 복합전단계수, 위상각을 구하였으며 재료의 동적특성으로부터 크리프 거동의 예측이 가능 하였다

• FOCUS: LIFE SCIENCE
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• 제1호
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• pp.1.1-1.4
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• 2024

This article provides comprehensive guidance on the maintenance, cleaning, regeneration, and storage of silica-based HPLC (High-Performance Liquid Chromatography) columns. The general considerations emphasize the importance of using in-line filters and guard cartridges to protect columns from blockage and irreversible sample adsorption. While these measures help, contamination by strongly adsorbed sample components can still occur over time, leading to an increase in back pressure, loss of efficiency, and other issues. To maximize column lifetime, especially with UHPLC (Ultra-High Performance Liquid Chromatography) columns, it is advisable to use ultra-pure solvents, freshly prepared aqueous mobile phases, and to filter all samples, standards, and mobile phases. Additionally, an in-line filter system and sample clean-up on dirty samples are recommended. However, in cases of irreversible compound adsorption or column voiding, regeneration may not be possible. The document also provides specific recommendations for column cleaning procedures, including the flushing procedures for various types of columns such as reversed phase, unbonded silica, bonded normal phase, anion exchange, cation exchange, and size exclusion columns for proteins. The flushing procedures involve using specific solvents in a series to clean and regenerate the columns. It is emphasized that the flow rate during flushing should not exceed the specified limit for the particular column, and the last solvent used should be compatible with the mobile phase. Furthermore, the article outlines the storage conditions for silica based HPLC columns, highlighting the impact of storage conditions on the column's lifetime. It is recommended to flush all buffers, salts, and ion-pairing reagents from the column before storage. The storage solvent should ideally match the one used in the initial column test chromatogram provided by the manufacturer, and column end plugs should be fitted to prevent solvent evaporation and drying out of the packing bed.

• 한국수소및신에너지학회논문집
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• 제35권3호
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• pp.280-289
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• 2024

In this study, a cryogenic vessel was constructed to maintain and control the safe pressure of liquid hydrogen boil-off gas (BOG), and the numerical analysis was conducted on the development of computational fluid dynamics model inside the high-pressure vessel. An evaluation system was constructed using cryogenic inner and outer containers, pre-cooler, upper flange, and internal high-pressure container. We attempted to analyze the performance of the safety valve by injecting relatively high temperature hydrogen gas to generate BOG gas and quickly control the pressure of the high-pressure vessel up to 10 bar. As a results, the liquid volume fraction decreased with a rapid evaporation, and the pressure distribution increased monotonically inside a high pressure vessel. Additionally, it was found that the time to reach 10 bar was greatly affected by the filling rate of liquid hydrogen.

• 센서학회지
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• 제32권3호
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• pp.140-146
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• 2023

Water quality is crucial for human health and the environment. Accurate measurement of the quantity of organic carbon in water is essential for water quality evaluation, identification of water pollution sources, and appropriate implementation of water treatment measures. Total organic carbon (TOC) analysis is an important tool for this purpose. Although other methods, such as chemical oxygen demand (COD) and biochemical oxygen demand (BOD) are also used to measure organic carbon in water, they have limitations that make TOC analysis a more favorable option in certain situations. For example, COD requires the use of toxic chemicals, and BOD is time-consuming and can produce inconsistent and unreliable results. In contrast, TOC analysis is rapid and reliable, providing accurate measurements of organic carbon content in water. However, common methods for TOC analysis can be complex and energy-intensive because of the use of high-temperature heaters for liquid-to-gas phase transitions and the use of acid, which present safety risks. This study focuses on a TOC analysis method using TiO₂ photocatalysis, which has several advantages over conventional TOC analysis methods, including its low cost and easy maintenance. For TiO₂, rutile and anatase powders are mixed with an inorganic binder and spray-coated onto a glass fiber substrate. The TiO₂ powder and inorganic binder solutions are adjusted to optimize the photocatalytic reaction performance. The TiO₂ photocatalysis method is a simple and low-power approach to TOC analysis, making it a promising alternative to commonly used TOC analysis methods. This study aims to contribute to the development of more efficient and cost-effective approaches for water quality analysis and management by exploring the effectiveness and reliability of the developed equipment.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1781-1789
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• 2016

Glargine insulin is a long-acting insulin analog that helps blood glucose maintenance in patients with diabetes. We constructed the pPT-GI vector to express prepeptide glargine insulin when transformed into Escherichia coli JM109. The transformed E. coli cells were cultured by fed-batch fermentation. The final dry cell mass was 18 g/l. The prepeptide glargine insulin was 38.52% of the total protein. It was expressed as an inclusion body and then refolded to recover the biological activity. To convert the prepeptide into glargine insulin, citraconylation and trypsin cleavage were performed. Using citraconylation, the yield of enzymatic conversion for glargine insulin increased by 3.2-fold compared with that without citraconylation. After the enzyme reaction, active glargine insulin was purified by two types of chromatography (ion-exchange chromatography and reverse-phase chromatography). We obtained recombinant human glargine insulin at 98.11% purity and verified that it is equal to the standard of human glargine insulin, based on High-performance liquid chromatography analysis and Matrix-assisted laser desorption/ionization Time-of-Flight Mass Spectrometry. We thus established a production process for high-purity recombinant human glargine insulin and a method to block Arg (B31)-insulin formation. This established process for recombinant human glargine insulin may be a model process for the production of other human insulin analogs.