• BMB Reports
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• v.48 no.2
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• pp.68-80
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• 2015

G protein-coupled receptors (GPCRs) are a large class of transmembrane receptors categorized into five distinct families: rhodopsin, secretin, adhesion, glutamate, and frizzled. They bind and regulate 80% of all hormones and account for 20-50% of the pharmaceuticals currently on the market. Hundreds of GPCRs integrate and coordinate the functions of individual cells, mediating signaling between various organs. GPCRs are crucial players in tumor progression, adipogenesis, and inflammation. Several studies have also confirmed their central roles in embryonic development and stem cell maintenance. Recently, GPCRs have emerged as key players in the regulation of cell survival, proliferation, migration, and self-renewal in pluripotent (PSCs) and cancer stem cells (CSCs). Our study and other reports have revealed that the expression of many GPCRs is modulated during the generation of induced PSCs (iPSCs) or CSCs as well as during CSC sphere formation. These GPCRs may have crucial roles in the regulation of self-renewal and other biological properties of iPSCs and CSCs. This review addresses the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PSCs or CSCs.

• Journal of Korean Society for Atmospheric Environment
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• v.28 no.4
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• pp.396-410
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• 2012

The purpose of this study is to quantify the contribution of high emitting vehicles to mobile emission inventories. Analyzed emission data include $NO_x$, HC, and CO results, which were measured through the vehicle Inspection and Maintenance (I/M) program in Seoul metropolitan area. The high emitting vehicles were identified as the top 5% worst polluting cars of the fleet. We estimated that 5% of the gasoline passenger car fleet, which is high emitters, generated 25.5% of $NO_x$, 34.5% of HC, and 66.1% of CO emissions of total inventories for gasoline passenger car fleet in year 2010. In the study, we identified that the older vehicles (older than ten years) and high mileage vehicles (more than 120,000 km driven) comprised high emitter fleet with 70.9% and 71.2%, respectively. The emission contribution of high emitters became larger in younger fleet than in the older fleet. This is due to the reduced emission rates in newly manufactured vehicles, which were developed under the more stringent emission regulation limits. This analysis implies that high emitters could be responsible for an even larger fraction of total vehicular emissions as more advanced technology vehicles are being incorporated into the current vehicle fleet. The findings suggested that the high emitting vehicles should be primarily considered for in-use vehicle emission management program, such as I/M, accelerated vehicle retirement, or catalytic converter replacement, in order to enhance the effectiveness of selected program.

• The Korean Journal of Physiology and Pharmacology
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• v.18 no.6
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• pp.517-524
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• 2014

Phasic and tonic ${\gamma}$-aminobutyric acidA ($GABA_A$) receptor-mediated inhibition critically regulate neuronal information processing. As these two inhibitory modalities have distinctive features in their receptor composition, subcellular localization of receptors, and the timing of receptor activation, it has been thought that they might exert distinct roles, if not completely separable, in the regulation of neuronal function. Inhibition should be maintained and regulated depending on changes in network activity, since maintenance of excitation-inhibition balance is essential for proper functioning of the nervous system. In the present study, we investigated how phasic and tonic inhibition are maintained and regulated by different signaling cascades. Inhibitory postsynaptic currents were measured as either electrically evoked events or spontaneous events to investigate regulation of phasic inhibition in layer 2/3 pyramidal neurons of the rat visual cortex. Tonic inhibition was assessed as changes in holding currents by the application of the $GABA_A$ receptor blocker bicuculline. Basal tone of phasic inhibition was maintained by intracellular $Ca^{2+}$ and $Ca^{2+}$/calmodulin-dependent protein kinase II (CaMKII). However, maintenance of tonic inhibition relied on protein kinase A activity. Depolarization of membrane potential (5 min of 0 mV holding) potentiated phasic inhibition via $Ca^{2+}$ and CaMKII but tonic inhibition was not affected. Thus, phasic and tonic inhibition seem to be independently maintained and regulated by different signaling cascades in the same cell. These results suggest that neuromodulatory signals might differentially regulate phasic and tonic inhibition in response to changes in brain states.

• Journal of Embryo Transfer
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• v.29 no.4
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• pp.375-383
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• 2014

Klotho (KL) is a single transmembrane protein composed of KL1 and KL2 repeats possessing ${\beta}$-glucuronidase activity and maintains calcium homeostasis in physiological state. It has been implicated in pigs that calcium is important for the establishment and maintenance of pregnancy, and our previous study has shown that transient receptor potential vanilloid type 6 (TRPV6), a calcium ion transporter, is predominantly expressed in the uterine endometrium during pregnancy in pigs. However, expression and function of KL in the uterine endometrium has not been determined in pigs. Thus, the present study determined expression and regulation of KL in the uterine endometrium during the estrous cycle and pregnancy in pigs. Real-time RT-PCR analysis showed that levels of KL mRNA decreased between Days 12 to 15 of the estrous cycle, and its expression showed a biphasic manner during pregnancy. KL mRNA was expressed in conceptuses and in chorioallantoic tissues during pregnancy. Explant culture study showed that expression levels of KL were not affected by treatment of steroid hormones or interleukin-1beta during the implantation period. Furthermore, levels of KL mRNA in the uterine endometrium from gilts carrying somatic cell nuclear transfer (SCNT)-derived embryos were significantly lower than those from gilts carrying natural mating-derived embryos on Day 12 of pregnancy. These results exhibited that KL was expressed at the maternal-conceptus interface in a pregnancy status- and stage-specific manner, and its expression was affected by SCNT procedure, suggesting that KL may play an important role in the establishment and maintenance of pregnancy in pigs.

• Journal of Korean Academy of Nursing
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• v.26 no.2
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• pp.413-427
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• 1996

This study was conducted to test the effect of a self regulation education program as a nursing intervention with chronically ill patients. A quasi experimental research(non equivalent control group pretest-posttest design) was used in this study. The subjects were 30 non insulin dependent diabetic patients(experimental group : 14 patients, control group : 16 patients). The study was carried out from May, 1995 to February, 1996. Data were collected before the education program, immediately after ＆ 2 menths later and were analyzed with repeated measure ANCOVA, paired t-test and t-test. The results are as follows : 1. There was a significant difference in self efficacy between the two groups（F＝27.61, P＝0.000）. There was a significant difference according to experimental stages（F＝33.09, P＝0.000） and interaction between education and experimental stages（F＝30.21, P＝0.000）. 2. There was a significant difference in self care behavior between the two groups（F＝27.05, P＝0. 000). There was a significant difference according to experimental stages（F＝31.14, P＝0.000） and interaction between education and experimental stages（F＝28.88, P＝0.000）. 3. There was a significant difference in glycemic control between before the education program and 2 months iater in the experimental group （t＝2.88, P＝0.013）. But there was no significant difference between before the education program and 2 months later in the control group. These results suggest that a self regulation education program is effective in promoting ＆ maintaining self care behavior and in improving glycemic control.. Thus this program can be recommended as an effective nursing intervention for chronically ill patients including diabetic patients.

• Journal of Electrical Engineering and Technology
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• v.12 no.5
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• pp.2031-2042
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• 2017

In this paper, a novel traffic flow control method based-on ramp metering and speed regulation using an adaptive sliding mode control (ASMC) method along with a deadzoned parameter adaptation law is proposed at a stochastic macroscopic level traffic environment, where the influence of the density and speed disturbances is accounted for in the traffic dynamic equations. The goal of this paper is to design a local traffic flow controller using both ramp metering and speed regulation based on ASMC, in order to achieve the desired density and speed for the maintenance of the maximum mainline throughput against disturbances in practice. The proposed method is advantageous in that it can improve the traffic flow performance compared to the traditional methods using only ramp metering, even in the presence of ramp storage limitation and disturbances. Moreover, a prior knowledge of disturbance magnitude is not required in the process of designing the controller unlike the conventional sliding mode controller. A stability analysis is presented to show that the traffic system under the proposed traffic flow control method is guaranteed to be uniformly bounded and its ultimate bound can be adjusted to be sufficiently small in terms of deadzone. The validity of the proposed method is demonstrated under different traffic situations (i.e., different initial traffic status), in the sense that the proposed control method is capable of stabilizing traffic flow better than the previously well-known Asservissement Lineaire d'Entree Autoroutiere (ALINEA) strategy and also feedback linearization control (FLC) method.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.21 no.3
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• pp.225-232
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• 2008

Overload vehicles operate damage to road, bridge, and then increasing in maintenance and repair cost because structures are reduced durability. The existing regulation systems have many problems and need coping measure. Therefore, this paper organized Ubiquitous sensor network system for development of intelligent auto overload vehicle regulation system about high speed vehicles, also axial load WIM sensor was selected by indoor experiment through wireless protocol. And we examined possibility U-load auto overload vehicle regulation system through experiment of the transmission and reception distance. If this system will apply to road and bridge, might be effective for economy and convenience through establishment of U-IT system. And high speed vehicle that was amalgamate IT technology and existing overload regulation problems, also tested wireless sensor for USN organization. This experiment aim to organize system interface for user through perfection man-less, wireless system of Internal/External Network from high speed WIN sensor with USN organization. Accordingly, it is necessary experimentation through Test Bed for constitution External network and application of actually regulations using WCDMA/HSDPA.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.6
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• pp.3587-3592
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• 2013

Aims: To study the CIK cell treatment effects on regulation of cellular immune function disorders in patients with lung cancer, and to analyze the time characteristics. Methods: Cellular immune function was assessed by FCM, and patients with functional disorders were randomly divided into two groups, one given CIK cell therapy within 18 months (5 courses) and the other the controls, which were followed up for 1 year with cellular immune functions tested once a month. Results: There were 5 types of cellular immunity, 4 of which are disorders; after CIK treatment, the improvement rate of the 4 groups were 79.1%, 70.8%, 76.0% and 70.0%, intergroup differences not being statistically significant (P=0.675), all significantly higher than in the control group (P=0.000). The median maintenance times for the 4 groups were 10.4 months (9.76-11.04), 8.4 months (7.86-8.94), 9.8 months (9.20-10.4) and 7.9 months (6.25-9.55), respectively. Conclusions: CIK cells were able to improve the immune functions of patients with lung cancer, the rate of improvement and maintenance time being related to the immune function before the treatment and CIK-cell-therapy courses.

• Journal of the Korean Society for Railway
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• v.15 no.2
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• pp.172-178
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• 2012

Superimposition of vertical and transition curves is not allowed for all types of tracks in Korean regulation of railway construction. However, this criterion has been established on the basis of difficulty of maintenance of the ballasted track. Comparing with the ballasted track, the concrete slab track has the advantage of maintenance and structural, which allows the superimposition of vertical and transition curves when it satisfies the criteria of running safety and riding comfort. In this study, running safety, ride comforts and track acting forces were analyzed and compared by numerical analysis using VAMPIRE program when the vertical and transition curves are superimposed for the ballasted track as well as the concrete slab track. From the analysis results, it was found that running safety, riding comforts and track acting forces in case of superimposition of vertical and transition curves for the both tracks meet all of the criteria. Therefore, although superimposition of vertical and transition curves for the ballasted track is not allowed because of a lot of maintenance works, it can be applied to the concrete slab track considering the advantage of maintenance and structural safety.

• BMB Reports
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• v.49 no.6
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• pp.311-318
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• 2016

Innate immune responses are primary, relatively limited, and specific responses to numerous pathogens and toxic molecules. Protein expression involved in these innate responses must be tightly regulated at both transcriptional level and post-transcriptional level to avoid the development of excessive inflammation that can be potentially harmful to the host. MicroRNAs are small noncoding RNAs (∼22 nucleotides [nts]) that participate in the regulation of numerous physiological responses by targeting specific messenger RNAs to suppress their translation. Recent work has shown that several negative regulators of transcription including microRNAs play important roles in inhibiting the exacerbation of inflammatory responses and in the maintenance of immunological homeostasis. This emerging research area will provide new insights on how microRNAs regulate innate immune signaling. It might show that dysregulation of microRNA synthesis is associated with the pathogenesis of inflammatory and infectious diseases. In this review, we focused on miR-146 and miR-125 and described the roles these miRNAs in modulating innate immune signaling. These microRNAs can control inflammatory responses and the outcomes of pathogenic infections.