• The Korean Journal of Physiology and Pharmacology
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• 제2권5호
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• pp.611-616
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• 1998

Although the $Ca^{2+}-activated\;K^+\;(I_{K,Ca})$ channel is known to play an important role in the maintenance of resting membrane potential, the regulation of the channel in physiological condition is not completely understood in vascular myocytes. In this study, we investigated the role of cytoplasmic $Mg^{2+}$ on the regulation of $I_{K,Ca}$ channel in pulmonary arterial myocytes of the rabbit using the inside-out patch clamp technique. $Mg^{2+}$ increased open probability (Po), but decreased the magnitude of single channel current. $Mg^{2+}-induced$ block of unitary current showed strong voltage dependence but increase of Po by $Mg^{2+}$ was not dependent on the membrane potential. The apparent effect of $Mg^{2+}$ might, thus, depend on the proportion between opposite effects on the Po and on the conductance of $I_{K,Ca}$ channel. In low concentration of cytoplasmic $Ca^{2+},\;Mg^{2+}$ increased $I_{K,Ca}$ by mainly enhancement of Po. However, at very high concentration of cytoplasmic $Ca^{2+},$ such as pCa 5.5, $Mg^{2+}$ decreased $I_{K,Ca}$ through the inhibition of unitary current. Moreover, $Mg^{2+}$ could activate the channel even in the absence of $Ca^{2+}.\;Mg^{2+}$ might, therefore, partly contribute to the opening of $I_{K,Ca}$ channel in resting membrane potential. This phenomenon might explain why $I_{K,Ca}$ contributes to the resting membrane potential where membrane potential and concentration of free $Ca^{2+}$ are very low.

• Genomics & Informatics
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• 제9권3호
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• pp.102-113
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• 2011

C-terminal SRC kinase (CSK) is a ubiquitously expressed, cytosolic enzyme that phosphorylates and inactivates several SRC family protein tyrosine kinases. Recent genomewide association studies have implicated CSK in the regulation of blood pressure. The current study aim is to determine the blood pressure association of the genes regulated by CSK down-regulation. The CSK mRNA expression was downregulated in vascular smooth muscle cells using small interfering RNA (siRNA). CSK mRNA levels fell by 90% in cells that were treated with CSK siRNA; the RNA from these cells was examined by microarray using the Illumina HumanRef-8 v3 platform, which comprises 24,526 reference mRNA probes. On treatment with CSK siRNA, 19 genes were downregulated by more than 2-fold and 13 genes were upregulated by more than 2-fold. Three (CANX, SLC30A7, and HMOX1) of them revealed more than 3 fold differential expression. Interestingly, the HMOX1 SNPs were associated with diastolic blood pressure in the 7551 Koreans using Korea Association REsource data, and the result was supported by the other reports that HMOX1 linked to blood vessel maintenance. Among the remaining 29 differentially expressed genes, seven (SSBP1, CDH2, YWHAE, ME2, PFTK1, G3BP2, and TUFT1) revealed association with both systolic and diastolic blood pressures. The CDH2 gene was linked to blood pressures. Conclusively, we identified 32 differentially expressed genes which were regulated by CSK reduction, and two (HOMX1 and CDH2) of them might influence the blood pressure regulation through CSK pathway.

• The Korean Journal of Physiology
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• 제29권1호
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• pp.91-102
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• 1995

The role of neurohumoral mechanisms in the regulation of cardiovascular functions and the effects of ethanol (EOH) on these mechanisms were examined in hemorrhaged conscious Wistar rats. The rats were bled at a constant rate (2 ml/kg/min) through the femoral artery until mean arterial pressure (MAP) was reduced by 30 mmHg. We studied the responses to hemorrhage 1) under normal conditions (Normal), and after pretreatments with 2) neural blockade (NB), pentolinium, 3) arginine vasopressin V1-receptor antagonist (AVPX) + NB, 4) angiotensin II ATI-receptor antagonist (AngIIX) + NB, 5) combined humoral blockade (HB), and 6) neurohumoral blockade. Intravenous administration of 30% EOH (6.3 ml/kg) attenuated the baroreceptor reflex sensitivity, and enhanced the depressor action of AngIIX. During hemorrhage, NB produced a faster fall ill MAP than Normal both in the saline and EOH groups. However, HB accelerated the rate of fall in MAP only in the EOH group. The recovery from hemorrhagic hypotension was not different between NB and Normal rats, but was attenuated in HB rats in the saline group. Under NB, AngIIX, but not AVPX, retarded the recovery rate compared with NB alone. EOH attenuated the recovery of MAP after hemorrhage in Normal rats, but completely abolished the recovery in HB rats. We conclude that 1) the maintenance of MAP during hemorrhage is mediated almost entirely by the autonomic functions, 2) angiotensin II plays an important role in the recovery from hemorrhagic hypotension, but AVP assumes little importance, 3) AVP release largely depends on the changes in blood volume, whereas renin release depends on the changes in blood pressure rather than blood volume, and 4) EOH increases the dependence of cardiovascular regulation on angiotensin II and impairs the recovery from hemorrhagic hypotension through the attenuation of autonomic functions.

• BMB Reports
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• 제55권6호
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• pp.287-292
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• 2022

The acute response to hypoxia is mainly driven by hypoxia-inducible factors, but their effects gradually subside with time. Hypoxia-specific histone modifications may be important for the stable maintenance of long-term adaptation to hypoxia. However, little is known about the molecular mechanisms underlying the dynamic alterations of histones under hypoxic conditions. We found that the phosphorylation of histone H3 at Ser-10 (H3S10) was noticeably attenuated after hypoxic challenge, which was mediated by the inhibition of aurora kinase B (AURKB). To understand the role of AURKB in epigenetic regulation, DNA microarray and transcription factor binding site analyses combined with proteomics analysis were performed. Under normoxia, phosphorylated AURKB, in concert with the repressor element-1 silencing transcription factor (REST), phosphorylates H3S10, which allows the AURKB-REST complex to access the MDM2 proto-oncogene. REST then acts as a transcriptional repressor of MDM2 and downregulates its expression. Under hypoxia, AURKB is dephosphorylated and the AURKB-REST complex fails to access MDM2, leading to the upregulation of its expression. In this study, we present a case of hypoxia-specific epigenetic regulation of the oxygen-sensitive AURKB signaling pathway. To better understand the cellular adaptation to hypoxia, it is worthwhile to further investigate the epigenetic regulation of genes under hypoxic conditions.

• 시큐리티연구
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• 제61호
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• pp.39-57
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• 2019

행정안전부장관의 '정부청사 보안관리'라는 권한행사는 조직법(정부조직법)만으로는 불충분하며, 이를 규율하기 위해서는 구체적인 내용과 한계를 설정하는 작용법(개별법)을 제정할 필요가 있다. 정부청사의 방호·보안 관련규정을 포함하고 있는 현행 「정부청사관리규정」(대통령령)은 법률상의 근거가 없는 법규명령으로 현 헌법체계와 부합되지 아니하며 그 법규성에 의문이 제기된다. 동 규정은 청사의 수급 및 관리, 청사의 취득 및 처분, 청사의 시설관리 등에 한정하여 규율하는 경우에는 공물관리법의 성격을 가진다고 할 수 있다. 그러나 동 규정은 '청사의 출입제한 및 통제' 등 시설보안 및 질서유지와 같은 행정청의 고권적 행위를 포함하고 있어 공물경찰법의 성격도 아울러 가지고 있다. 이에 대한 입법개선 방안으로 청사의 수급·배정 등을 규율하고 있는 「정부청사관리규정」은 그대로 유지하되, 청사의 출입관리 등 행정주체의 고권적 행위를 규정하고 있는 방호·보안관리와 관련된 조문은 법률로 상향 조정할 필요가 있다. 또한 정부청사 방호직무 수행자인 방호관(일반직공무원)의 법률상 권한 부재로 현장 방호직무 수행 간 대응력 확보에 한계가 있어 근거법 명시를 통한 방호인력의 직무권한 확보가 요청된다. 정부청사 방호·보안 관련 법률의 주요내용은 출입자의 행위제한, 방호인력의 직무권한 및 의무, 무기의 사용, 방호인력에 대한 교육·훈련, 벌칙 등과 같이 국민의 권리제한과 의무부담과 관계되는 법률유보사항은 개별법에 반드시 포함될 필요가 있다.

• Molecules and Cells
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• 제44권3호
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• pp.168-178
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• 2021

The retinal pigment epithelium (RPE) forms a monolayer sheet separating the retina and choroid in vertebrate eyes. The polarized nature of RPE is maintained by distributing membrane proteins differentially along apico-basal axis. We found the distributions of these proteins differ in embryonic, post-natal, and mature mouse RPE, suggesting developmental regulation of protein trafficking. Thus, we deleted tumor susceptibility gene 101 (Tsg101), a key component of endosomal sorting complexes required for transport (ESCRT), in embryonic and mature RPE to determine whether ESCRT-mediated endocytic protein trafficking correlated with the establishment and maintenance of RPE polarity. Loss of Tsg101 severely disturbed the polarity of RPE, which forms irregular aggregates exhibiting non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling. These findings suggest that ESCRT-mediated protein trafficking is essential for the development and maintenance of RPE cell polarity.

• Molecules and Cells
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• 제46권7호
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• pp.441-450
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• 2023

β-Catenin (Ctnnb1) has been shown to play critical roles in the development and maintenance of epithelial cells, including the retinal pigment epithelium (RPE). Ctnnb1 is not only a component of intercellular junctions in the epithelium, it also functions as a transcriptional regulator in the Wnt signaling pathway. To identify which of its functional modalities is critically involved in mouse RPE development and maintenance, we varied Ctnnb1 gene content and activity in mouse RPE lineage cells and tested their impacts on mouse eye development. We found that a Ctnnb1 double mutant (Ctnnb1^dm), which exhibits impaired transcriptional activity, could not replace Ctnnb1 in the RPE, whereas Ctnnb1^Y654E, which has reduced affinity for the junctions, could do so. Expression of the constitutively active Ctnnb1^∆ex3 mutant also suppressed the development of RPE, instead facilitating a ciliary cell fate. However, the post-mitotic or mature RPE was insensitive to the loss, inactivation, or constitutive activation of Ctnnb1. Collectively, our results suggest that Ctnnb1 should be maintained within an optimal range to specify RPE through transcriptional regulation of Wnt target genes in the optic neuroepithelium.

• 지적과 국토정보
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• 제52권1호
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• pp.17-36
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• 2022

본 연구의 목적은 현행 개발제한구역 단속 및 관리체계의 문제점을 진단하고 데이터 기반 모니터링 체계로 전환할 수 있도록 구축방안을 마련하는 것이다. 개발제한구역은 타 용도지역지구와 달리 최소한의 유지 외 모든 개발행위를 금지하고 지자체에서 엄격히 단속 및 관리해야 하는 구역이다. 그러나 현재의 개발제한구역 관리체계는 지자체별 여건에 따라 분산 운영되고, 설문 결과와 같이 전체 개발제한구역의 변화를 모니터링하기보다 민원(신고)에 의존하거나 단속업무 수행에 어려움을 겪는 등 데이터 기반의 모니터링체계를 필요로 하고 있다. 특히 본 연구에서는 AI 기반 모니터링체계를 도입하여 국토교통부에서 매년 정기적 시점(월별·분기별)을 두고 동일한 단속 기준으로 전국 권역별 변화를 탐지한 결과를 지자체에 송부하고 지자체에서는 단속 결과를 입력하면서 신뢰할 수 있는 개발제한구역 관리현황 통계정보가 생산될 수 있는 체계로의 전환 방법을 구체적으로 제안하였다. 이러한 방법론 제안을 위해 첫째, 지자체와 관련 전문가를 대상으로 설문조사 및 인터뷰를 수행하였다. 설문 결과 현장 단속과 인허가 관련 행정업무에 어려움을 겪고 있으며, AI 기반 모니터링체계 도입에 대해서는 긍정적 답변이 우세하였다. 둘째, AI 영상분석을 통한 객체 검출 방법론을 실증 지자체에 적용한 사례를 분석하여 모니터링체계 도입에 따른 단속업무 효율화 방안을 제안하였다. 셋째, (현(現))개발제한구역관리정보시스템의 고도화를 기반으로 한 개발제한구역 모니터링체계 구축방안을 마련하였다. 지자체업무 수요에 기반해 드론 촬영 및 분석, 모바일 단속지원체계 등 필요한 서비스들이 지원될 수 있도록 프레임워크를 구성하였다. 이러한 모니터링체계는 향후 주기적 단속과 관리가 필요한 토지를 대상으로 확대 적용이 가능하며 본 연구에서는 이를 실현하기 위한 방법론과 정책을 제안하고자 하였다.

• 한국가스학회지
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• 제20권5호
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• pp.57-63
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• 2016

밸브는 설비 및 시스템의 제어를 위한 필수 요소로써 필수부품이지만 사용 중 Stem에서부터 볼까지의 연결부 유격으로 인한 개폐 불량이 발생할 수 있으며 이물질 유입, 마모, 크랙, 스크래치, 고착, 작동불량 등으로 인하여 사고로까지 이어질 수 있다. 사례 조사 결과 밸브의 고장 원인은 64%가 기밀누설이나 작동불량이었으며, 대부분의 밸브 누출 폭발사고는 설치현장에서 밸브 고착 등 작동불량을 해소하는 과정에서 발생했다. 이는 안전관리와 유지관리에 대한 시스템이 미비하고 안전밸브를 제외한 산업용 밸브의 유지관리 의무화가 되는 법규가 없어 권장사항과 유지관리 업체의 재량에 맡기고 있는 실정이기 때문이다. 따라서 철저한 유지관리로 위험요인을 제거하고 누출 사고 발생 시 피해의 최소화를 위해 초동대처방안이 마련되어야 한다. 본 연구에서는 산업현장에서 밸브의 사용 현황과 유지관리현황을 조사하고 밸브 모니터링을 통한 안전성 향상 방안을 제시하였다. 밸브상태진단 및 운영조회, 가스누출상시감시, 밸브 유지관리 비용절감, 전주기 수명관리 등의 효과를 갖는 모니터링 시스템 구축으로 가스안전관리 고도화에 기여할 수 있을 것이다.

• 환경위생공학
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• 제24권1호
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• pp.1-12
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• 2009

The various environmental problems that we face on today are basically about environmental quality. Since early 1960s affluent material was produced because of remarkable economic growth by many cooperations. However, for the lack of environmental policy, environmental pollutions has been serious. The central government should transfer the producing and consuming structure to environmental affinity through the regulations for developing Korea as a model of environmental nation which takes an active part in global environmental programme and in which the environment and economy are well harmonized. Moreover, the central government should take the lead in prevention of environmental pollution through the direct policies such as strengthening the discharge limit or setting up environmental basic institutions by securing budget for conserving environment. This thesis emphasize on the public institution among many environmental basic institutions for environmental anti-pollution project. It will find the problems with running those institutions, and will suggest the preview of improvement. Also, it is necessary to investigate of variation trend for inflow and pollutant loading to environmental infrastructure as increased of the diffusion rate as established and maintenance of sewer system. The purpose of this study is to investigate for inflow and pollutant loading to environmental infrastructure, and also to provide the method of efficiently maintenance and management. The results obtained were summarized as follows; 1. Survey of actual condition on operation and management of environmental infrastructure was evaluated the propriety of treatment process and problem of plant management. 2. Analysis of pollutant loading contribution for river system of environmental infrastructure with data analysis of water quality measuring network. 3. To investigate on case study for efficiently maintenance and management of environmental infrastructure. The result on this study was provide the method of efficiently maintenance and management with survey for establish and repair of sewer system and survey of actual condition on operation and management of environmental infrastructure in the water area of discharge to Geum River System. Application as guideline for establish and management of environmental infrastructure, and management of Geum River System. Also, application for preliminary data for fulfill-assess of total effluent regulation of water pollution.