• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1781-1789
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• 2016

Glargine insulin is a long-acting insulin analog that helps blood glucose maintenance in patients with diabetes. We constructed the pPT-GI vector to express prepeptide glargine insulin when transformed into Escherichia coli JM109. The transformed E. coli cells were cultured by fed-batch fermentation. The final dry cell mass was 18 g/l. The prepeptide glargine insulin was 38.52% of the total protein. It was expressed as an inclusion body and then refolded to recover the biological activity. To convert the prepeptide into glargine insulin, citraconylation and trypsin cleavage were performed. Using citraconylation, the yield of enzymatic conversion for glargine insulin increased by 3.2-fold compared with that without citraconylation. After the enzyme reaction, active glargine insulin was purified by two types of chromatography (ion-exchange chromatography and reverse-phase chromatography). We obtained recombinant human glargine insulin at 98.11% purity and verified that it is equal to the standard of human glargine insulin, based on High-performance liquid chromatography analysis and Matrix-assisted laser desorption/ionization Time-of-Flight Mass Spectrometry. We thus established a production process for high-purity recombinant human glargine insulin and a method to block Arg (B31)-insulin formation. This established process for recombinant human glargine insulin may be a model process for the production of other human insulin analogs.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.189-196
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• 2017

Kaposi's sarcoma-associated herpesvirus (KSHV) is associated with formation of Kaposi's sarcoma, multicentric Castleman's disease, and primary effusion lymphoma. Replication and transcription activator (RTA) genes are expressed upon reactivation of KSHV, which displays a biphasic life cycle consisting of latent and lytic replication phases. RTA protein expression results in KSHV genome amplification and successive viral lytic gene expression. Transcriptional activity of viral lytic genes is regulated through epigenetic modifications. In Raji cells latently infected with Epstein-Barr virus, various modifications, such as acetylation and methylation, have been identified at specific lysine residues in histone H4 during viral reactivation, supporting the theory that expression of specific lytic genes is controlled by histone modification processes. Data obtained from chromatin immunoprecipitation and quantitative real-time PCR analyses revealed alterations in the H4K8ac and H4K20me3 levels at lytic gene promoters during reactivation. Our results indicate that H4K20me3 is associated with the maintenance of latency, while H4K8ac contributes to KSHV reactivation in infected TREx BCBL-1 RTA cells.

• Magazine of the Korean Society of Agricultural Engineers
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• v.39 no.4
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• pp.55-63
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• 1997

Constructed wetland system which can be applied to the rural wastewater treatment system was examined by pilot plant in Kon-Kuk University. Hydraulic loading rate of wastewater was about 0.16m$^3$/m$^2$. day and theoretical detention time in the system was 1.38 days. The effluent of the septic tank for the school building was applied as inflow to the system. The influent concentration of DO was zero but effluent was up to 4.37mg/${\ell}$ which implies that oxygen was supplied enough from atmosphere by reaeration to support biological activity of the system. Average influent concentration of BOD was 104mg/${\ell}$ and effluent was 24mg/${\ell}$ with average removal rate of 76%. Average influent concentration of COD was 215mg/${\ell}$ and effluent was 63mg/${\ell}$ with average removal rate of 70 % . Average influent concentration of SS was 78mg/${\ell}$ and effluent was 10mg/${\ell}$ with average removal rate of 87%. Two components, BOD and SS, are regulated by law to keep maximum water quality standard of 80mg/${\ell}$ when daily outflow rate is less than 100$m^3$/day which is the case of most rural communities. Therefore, the results from the experiment showed that constructed wetland system can meet the water quality standard easily. Average influent concentration of total nitrogen was 165mg/lwhich is relatively higher than normal wastewater, and effluent was about 156mg/${\ell}$ with average removal rate of only 6%. Average influent concentration of total phosphorus was 41 mg/${\ell}$ and effluent was 6mg/${\ell}$ with average removal rate of 87%. Overall, constructed wetland system was thought to be effective to treat wastewater if nitrogen removal mechanism is improved. Considering low cost, less maintenance, and high treatability, this system can be a practical alternative for the wastewater treatment in rural area The experiment was performed during the summer and fall season, and treatment efficiency of the system is expected to decrease in low temperature. therefore, further study including temperature is required to evaluate feasibility of the system more in detail.

• Journal of Microbiology and Biotechnology
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• v.19 no.12
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• pp.1557-1564
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• 2009

Human CDX2 is known as a caudal-related homeodomain transcription factor that is expressed in the intestinal epithelium and is important in differentiation and maintenance of the intestinal epithelial cells. The caudal-related homeobox proteins bind DNA according to a helix-turn-helix structure, thereby increasing the structural stability of DNA. A cancer-tumor suppressor role for Cdx2 has been shown by a decrease in the level of the expression of Cdx2 in colorectal cancer, but the mechanism of transcriptional regulation has not been examined at the molecular level. We developed a large-scale system for expression of the recombinant, novel CDX2, in Escherichia coli. A highly purified and soluble CDX2 protein was obtained in E. coli strain BL21(DE3)RIL and a hexahistidine fusion system using Ni-NTA affinity column, anion exchange, and gel filtration chromatographies. The identity and secondary structure of the purified CDX2 protein were confirmed by MALDI-TOF MS, Western blot, and a circular dichroism analyses. In addition, we studied the DNA-binding activity of recombinant CDX2 by ELISA experiment and isolated human CDX2-binding proteins derived from rat cells by an immobilized GST-fusion method. Three CDX2-binding proteins were found in the gastric tissue, and those proteins were identified to the homeobox protein Hox-D8, LIM homeobox protein 6, and SMC1L1 protein.

• Journal of the Korea Computer Industry Society
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• v.4 no.4
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• pp.633-646
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• 2003

Using the self-contained characteristics of XML, in various areas the XML is widely used to a meta language which can describe information and is recognized to a standard language to present information on the web. In this paper, we point out many difficult problems when we are going to a serial activity of software engineering like that we analyze, reuse, and maintenance source programs. Then we describe whether the existed studies about documentation provide the solutions for the problems above mentioned or not. Finally, we propose the JML(Java Markup Language) that can effectively support solutions about these difficulties. Then we analyze the sample program and present the generated JML docuement using the results. explain the result that The JML is an XML DTD to describe software information written by Java language. Also, we describe the meaning of elements that are parts of whole JML, and explain how to represent the information of Java source codes using each element.

• Journal of Korean Physical Therapy Science
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• v.16 no.2
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• pp.1-9
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• 2009

Background: The ability for backward walking is considered to be necessary for the neuromuscular control and maintenance of balance in daily ambulatory activity. This study aimed to determine the effect of backward walking training on the walking speed and balance control in patients with hemiplegia. Methods: Fourteen patients with hemiplegia were randomly allocated to an experimental and control groups of seven patients each. For the experimental group, we performed both conventional training and backward walking training, and conventional training only for the control group. The conventional training programs for the 2 groups were conducted for 30 min, twice a day, 5 times a week for 4 weeks, and backward walking training for the experimental group was conducted for 30 min, 3 times a week. The outcomes were assessed using the functional reach test (FRT), timed up-and-go (TUG) Test, and the 10 meter walk time test (10mWT). Result: A comparison of the FRT, TUG test, and 10mWT scores obtained before and after the 4-week treatment revealed statistically significant differences (p<.05) for the experimental group; however, there was no such difference in the case of the control group (p>.05). On assessment after the 4-week treatment, statistically significant differences were noted in the TUG test and 10mWT scores of the experimental group (p<.05). Conclusion: Our findings suggest that backward walking training is an effective clinical strategy for improving the walking speed and functional mobility of patients with hemiplegia.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.403-409
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• 2010

Saccharomyces cerevisiae Hsp30 is a plasma membrane heat shock protein that is induced by various environmental stress conditions. However, the functional role of Hsp30 during diverse environmental stressors is not presently known. To gain insight into its function during thermal stress, we have constructed and characterized a ${\Delta}hsp30$ strain during heat stress. $BY4741{\Delta}hsp30$ cells were found to be more sensitive compared with BY4741 cells, when exposed to a lethal heat stress at $50^{\circ}C$. When budding yeast is exposed to either heat shock or weak organic acid, it inhibits Pma1p activity. In this study, we measured the levels of Pma1p in mutant and Wt cells both during optimal temperature and heat shock temperature. We observed that $BY4741{\Delta}hsp30$ cells showed constitutive reduction of Pma1p. To gain further insights into the role of Hsp30 during heat stress, we compared the total protein profile by 2D gel electrophoresis followed by identification of differentially expressed spots by LC-MS. We observed that contrary to that expected from thermal-stress-induced changes in gene expression, the ${\Delta}hsp30$ mutant maintained elevated levels of Pdc1p, Trx1p, and Nbp35p and reduced levels of Atp2p and Sod1p during heat shock. In conclusion, Hsp30 is necessary during lethal heat stress, for the maintenance of Pma1p and a set of thermal stress response functions.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.501-507
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• 2017

The heavy metal such as Nikel (Ni) in industrial wastewater is one of the major reasons of decreasing nitrification efficiency in municipal wastewater treatment plants (MWTPs). In this study, laboratory scale reactors were operated in order to analyse of nitrification efficiency and improvement measures. As a result, nitrification efficiency during high Ni concentration (0.295 mg/L) was about 20%. However nitrification efficiency during low Ni concentration (0.114 mg/L) was over 70%. The changes of the micro-organism activity according to Ni concentration was investigated as being the major reason behind the gap of nitrification efficiency through analysing AUR and SNR. Increasing the HRT in high Ni concentration also increased the nitrification efficiency. Thus, maintenance of microorganisms and increasing the HRT in nitrification reactors suggests that measures taken to treat wastewater is positively correlated with high concentration of heavy metal.

• Physical Therapy Korea
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• v.25 no.1
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• pp.62-70
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• 2018

Background: Sitting posture influences movements of scapulothoracic and glenohumeral joints and changes the shoulder muscle activities. The development and maintenance of correct sitting posture is important for the fundamental treatment of shoulder pain during rehabilitation. Objects: The purpose of this study was to investigate the effects of the sitting postures and the shoulder movements on shoulder muscle activities for both male and female. Methods: Twenty-eight subjects without shoulder-related diseases participated in this experiment. The subjects had randomly adopted three different sitting postures (upright posture, preferred posture, maximum slouched posture) and shoulder flexion angles in scapular plane ($30^{\circ}$, $90^{\circ}$, $120^{\circ}$). Surface electrodes were collected from upper trapezius (UT), anterior deltoid (AD), and posterior deltoid (PD) and the active shoulder range of motion was measured in each sitting posture and shoulder flexion angle. Results: The active range of motions of the shoulder external rotation and the flexion in the scapular plane decreased from the upright posture to the maximum slouched posture (p<.05, mixed-effect linear regression with random intercept, Tukey post-hoc analysis). All muscles showed the highest EMG activities at $120^{\circ}$ shoulder flexion with the maximum slouched posture and did not show the gender differences. Conclusion: Increased shoulder muscle activities may become the potential risk factor for the shoulder impairment and pain if people continuously maintain the maximum slouched posture. Therefore, an upright position is necessary during shoulder exercises, as well as in activities of daily living, including motions involving lifting the arms.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.6
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• pp.1351-1359
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• 2003

Extreme programming is the most representative methodology among agile software development methodologies which is agile in business environment which change fast. As software industry is matured, usability of software quality characteristics is emphasized gradually, but effort to obtain usability in extreme programming is insufficient. To improve usability of extreme programming, in this thesis, a software life cycle is defined by six phase as exploration, planning, iterations to release, productionizing, maintenance, and death phase. In this thesis, an extreme programming process which can improve usability is proposed by definition of 11 activities including integration testing and acceptance testing for usability testing, The proposed process is validated with four aspects. The proposed process is enhanced to support acceptance testing activity. The proposed extreme programming process is able to support CMM level 4 and quality assurance practice of CMM.